Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5090 and GFF5091 overlap by 4 nucleotides GFF5091 and GFF5092 overlap by 4 nucleotides GFF5092 and GFF5093 overlap by 4 nucleotides
GFF5090 - Homoprotocatechuate degradative operon repressor, at 193,738 to 194,175
GFF5090
GFF5091 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 194,172 to 194,804
GFF5091
GFF5092 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 194,801 to 195,583
GFF5092
GFF5093 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 195,580 to 197,043
GFF5093
Position (kb) 194
195
196 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 194.081 kb on - strand, within GFF5090 at 194.081 kb on - strand, within GFF5090 at 194.129 kb on - strand, within GFF5090 at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.208 kb on + strand at 194.208 kb on + strand at 194.208 kb on + strand at 194.209 kb on - strand at 194.209 kb on - strand at 194.209 kb on - strand at 194.274 kb on + strand, within GFF5091 at 194.275 kb on - strand, within GFF5091 at 194.822 kb on + strand at 194.822 kb on + strand at 194.822 kb on + strand at 194.823 kb on - strand at 194.823 kb on - strand at 194.823 kb on - strand at 195.116 kb on + strand, within GFF5092 at 195.117 kb on - strand, within GFF5092 at 195.117 kb on - strand, within GFF5092 at 195.117 kb on - strand, within GFF5092 at 195.179 kb on + strand, within GFF5092 at 195.198 kb on - strand, within GFF5092 at 195.215 kb on + strand, within GFF5092 at 195.215 kb on + strand, within GFF5092 at 195.215 kb on + strand, within GFF5092 at 195.216 kb on - strand, within GFF5092 at 195.277 kb on + strand, within GFF5092 at 195.277 kb on + strand, within GFF5092 at 195.425 kb on + strand, within GFF5092 at 195.628 kb on + strand at 195.629 kb on - strand at 195.629 kb on - strand at 195.629 kb on - strand at 195.854 kb on + strand, within GFF5093 at 195.854 kb on + strand, within GFF5093 at 195.901 kb on + strand, within GFF5093 at 195.902 kb on - strand, within GFF5093 at 195.902 kb on - strand, within GFF5093 at 195.905 kb on - strand, within GFF5093 at 195.943 kb on + strand, within GFF5093 at 195.944 kb on - strand, within GFF5093 at 195.944 kb on - strand, within GFF5093 at 195.967 kb on + strand, within GFF5093 at 195.968 kb on - strand, within GFF5093 at 195.968 kb on - strand, within GFF5093 at 196.567 kb on + strand, within GFF5093 at 196.568 kb on - strand, within GFF5093
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 194,081 - GFF5090 0.78 -1.2 194,081 - GFF5090 0.78 -1.9 194,129 - GFF5090 0.89 +0.7 194,177 + -0.2 194,177 + +0.0 194,177 + +0.4 194,177 + -0.8 194,177 + +0.4 194,177 + +1.2 194,177 + +0.1 194,177 + -0.7 194,178 - -0.7 194,178 - +0.0 194,178 - -1.9 194,178 - -0.9 194,178 - +0.3 194,208 + -1.1 194,208 + +0.9 194,208 + -0.6 194,209 - -0.2 194,209 - -0.0 194,209 - +0.1 194,274 + GFF5091 0.16 +0.8 194,275 - GFF5091 0.16 -0.5 194,822 + -1.4 194,822 + +0.8 194,822 + -0.7 194,823 - -0.0 194,823 - +1.9 194,823 - +0.2 195,116 + GFF5092 0.40 +0.6 195,117 - GFF5092 0.40 -0.3 195,117 - GFF5092 0.40 +2.3 195,117 - GFF5092 0.40 +0.4 195,179 + GFF5092 0.48 +0.4 195,198 - GFF5092 0.51 +1.9 195,215 + GFF5092 0.53 +0.3 195,215 + GFF5092 0.53 -0.4 195,215 + GFF5092 0.53 -0.3 195,216 - GFF5092 0.53 +0.5 195,277 + GFF5092 0.61 -0.6 195,277 + GFF5092 0.61 +0.5 195,425 + GFF5092 0.80 +0.2 195,628 + +0.4 195,629 - -0.7 195,629 - -0.4 195,629 - +0.2 195,854 + GFF5093 0.19 -1.3 195,854 + GFF5093 0.19 -1.1 195,901 + GFF5093 0.22 -0.2 195,902 - GFF5093 0.22 -0.4 195,902 - GFF5093 0.22 +1.3 195,905 - GFF5093 0.22 +0.5 195,943 + GFF5093 0.25 +2.9 195,944 - GFF5093 0.25 +0.1 195,944 - GFF5093 0.25 +0.7 195,967 + GFF5093 0.26 +1.2 195,968 - GFF5093 0.27 -0.3 195,968 - GFF5093 0.27 -0.7 196,567 + GFF5093 0.67 +1.1 196,568 - GFF5093 0.67 +1.3
Or see this region's nucleotide sequence