Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF508 and GFF509 are separated by 16 nucleotides GFF509 and GFF510 overlap by 4 nucleotides GFF510 and GFF511 overlap by 4 nucleotides GFF511 and GFF512 overlap by 4 nucleotides
GFF508 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 549,582 to 550,649
GFF508
GFF509 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 550,666 to 551,646
GFF509
GFF510 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 551,643 to 552,371
GFF510
GFF511 - no description, at 552,368 to 552,514
GFF511
GFF512 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 552,511 to 553,278
GFF512
Position (kb) 550
551
552 Strain fitness (log2 ratio) -2
-1
0
1
2 at 549.754 kb on + strand, within GFF508 at 549.754 kb on + strand, within GFF508 at 549.755 kb on - strand, within GFF508 at 549.799 kb on + strand, within GFF508 at 549.799 kb on + strand, within GFF508 at 549.800 kb on - strand, within GFF508 at 549.883 kb on + strand, within GFF508 at 549.884 kb on - strand, within GFF508 at 549.958 kb on + strand, within GFF508 at 549.958 kb on + strand, within GFF508 at 549.959 kb on - strand, within GFF508 at 549.959 kb on - strand, within GFF508 at 550.093 kb on + strand, within GFF508 at 550.093 kb on + strand, within GFF508 at 550.093 kb on + strand, within GFF508 at 550.093 kb on + strand, within GFF508 at 550.093 kb on + strand, within GFF508 at 550.093 kb on + strand, within GFF508 at 550.094 kb on - strand, within GFF508 at 550.094 kb on - strand, within GFF508 at 550.094 kb on - strand, within GFF508 at 550.096 kb on + strand, within GFF508 at 550.096 kb on + strand, within GFF508 at 550.096 kb on + strand, within GFF508 at 550.096 kb on + strand, within GFF508 at 550.096 kb on + strand, within GFF508 at 550.097 kb on - strand, within GFF508 at 550.097 kb on - strand, within GFF508 at 550.411 kb on + strand, within GFF508 at 550.412 kb on - strand, within GFF508 at 550.463 kb on - strand, within GFF508 at 550.685 kb on + strand at 550.686 kb on - strand at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.772 kb on + strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 550.773 kb on - strand, within GFF509 at 551.647 kb on - strand at 551.647 kb on - strand at 551.647 kb on - strand at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.316 kb on + strand at 552.317 kb on - strand at 552.375 kb on + strand at 552.375 kb on + strand at 552.376 kb on - strand at 552.542 kb on + strand at 552.542 kb on + strand at 552.542 kb on + strand at 552.543 kb on - strand at 552.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 549,754 + GFF508 0.16 -1.0 549,754 + GFF508 0.16 -0.3 549,755 - GFF508 0.16 -0.8 549,799 + GFF508 0.20 -0.8 549,799 + GFF508 0.20 -1.2 549,800 - GFF508 0.20 +0.5 549,883 + GFF508 0.28 +0.5 549,884 - GFF508 0.28 +0.8 549,958 + GFF508 0.35 +0.2 549,958 + GFF508 0.35 -0.6 549,959 - GFF508 0.35 -0.2 549,959 - GFF508 0.35 +0.5 550,093 + GFF508 0.48 +0.3 550,093 + GFF508 0.48 -2.4 550,093 + GFF508 0.48 +0.8 550,093 + GFF508 0.48 +1.0 550,093 + GFF508 0.48 +0.9 550,093 + GFF508 0.48 +0.3 550,094 - GFF508 0.48 +0.3 550,094 - GFF508 0.48 +0.7 550,094 - GFF508 0.48 -2.4 550,096 + GFF508 0.48 -2.4 550,096 + GFF508 0.48 -1.9 550,096 + GFF508 0.48 -0.2 550,096 + GFF508 0.48 -0.6 550,096 + GFF508 0.48 -1.1 550,097 - GFF508 0.48 -0.7 550,097 - GFF508 0.48 -0.5 550,411 + GFF508 0.78 +0.0 550,412 - GFF508 0.78 +1.1 550,463 - GFF508 0.82 +2.3 550,685 + +1.3 550,686 - -1.0 550,772 + GFF509 0.11 -1.0 550,772 + GFF509 0.11 +0.5 550,772 + GFF509 0.11 +0.3 550,772 + GFF509 0.11 +0.8 550,772 + GFF509 0.11 -1.5 550,772 + GFF509 0.11 -0.0 550,773 - GFF509 0.11 +0.1 550,773 - GFF509 0.11 +1.3 550,773 - GFF509 0.11 -1.7 550,773 - GFF509 0.11 +0.0 550,773 - GFF509 0.11 -0.0 550,773 - GFF509 0.11 +0.4 550,773 - GFF509 0.11 -1.0 550,773 - GFF509 0.11 -0.8 551,647 - -0.2 551,647 - +0.2 551,647 - -0.5 552,118 + GFF510 0.65 +0.3 552,118 + GFF510 0.65 -1.3 552,118 + GFF510 0.65 -2.0 552,118 + GFF510 0.65 +1.3 552,119 - GFF510 0.65 -0.5 552,119 - GFF510 0.65 -1.9 552,119 - GFF510 0.65 -0.3 552,119 - GFF510 0.65 +0.3 552,316 + -0.6 552,317 - -0.2 552,375 + +1.2 552,375 + +1.0 552,376 - -0.9 552,542 + -0.4 552,542 + +0.3 552,542 + -0.0 552,543 - -0.3 552,543 - -0.6
Or see this region's nucleotide sequence