Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5002 and GFF5003 overlap by 11 nucleotides GFF5003 and GFF5004 are separated by 7 nucleotides
GFF5002 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 101,611 to 102,741
GFF5002
GFF5003 - FIG00348156: hypothetical protein, at 102,731 to 103,825
GFF5003
GFF5004 - N-acyl-L-amino acid amidohydrolase (EC 3.5.1.14), at 103,833 to 105,035
GFF5004
Position (kb)
102
103
104 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.794 kb on + strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.795 kb on - strand, within GFF5002 at 101.848 kb on + strand, within GFF5002 at 101.849 kb on - strand, within GFF5002 at 101.849 kb on - strand, within GFF5002 at 101.917 kb on + strand, within GFF5002 at 101.917 kb on + strand, within GFF5002 at 101.917 kb on + strand, within GFF5002 at 101.918 kb on - strand, within GFF5002 at 101.918 kb on - strand, within GFF5002 at 101.918 kb on - strand, within GFF5002 at 101.918 kb on - strand, within GFF5002 at 101.918 kb on - strand, within GFF5002 at 102.052 kb on + strand, within GFF5002 at 102.052 kb on + strand, within GFF5002 at 102.052 kb on + strand, within GFF5002 at 102.052 kb on + strand, within GFF5002 at 102.053 kb on - strand, within GFF5002 at 102.053 kb on - strand, within GFF5002 at 102.136 kb on + strand, within GFF5002 at 102.136 kb on + strand, within GFF5002 at 102.137 kb on - strand, within GFF5002 at 102.295 kb on + strand, within GFF5002 at 102.295 kb on + strand, within GFF5002 at 102.295 kb on + strand, within GFF5002 at 102.295 kb on + strand, within GFF5002 at 102.295 kb on + strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.296 kb on - strand, within GFF5002 at 102.341 kb on - strand, within GFF5002 at 102.448 kb on + strand, within GFF5002 at 102.448 kb on + strand, within GFF5002 at 102.448 kb on + strand, within GFF5002 at 102.448 kb on + strand, within GFF5002 at 102.448 kb on + strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.449 kb on - strand, within GFF5002 at 102.541 kb on + strand, within GFF5002 at 102.541 kb on + strand, within GFF5002 at 102.541 kb on + strand, within GFF5002 at 102.541 kb on + strand, within GFF5002 at 102.541 kb on + strand, within GFF5002 at 102.542 kb on - strand, within GFF5002 at 102.542 kb on - strand, within GFF5002 at 102.542 kb on - strand, within GFF5002 at 102.542 kb on - strand, within GFF5002 at 102.542 kb on - strand, within GFF5002 at 102.814 kb on + strand at 102.815 kb on - strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.816 kb on + strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.817 kb on - strand at 102.939 kb on + strand, within GFF5003 at 102.939 kb on + strand, within GFF5003 at 102.940 kb on - strand, within GFF5003 at 102.940 kb on - strand, within GFF5003 at 102.960 kb on + strand, within GFF5003 at 102.960 kb on + strand, within GFF5003 at 102.961 kb on - strand, within GFF5003 at 102.961 kb on - strand, within GFF5003 at 102.961 kb on - strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.984 kb on + strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 102.985 kb on - strand, within GFF5003 at 103.038 kb on + strand, within GFF5003 at 103.038 kb on + strand, within GFF5003 at 103.038 kb on + strand, within GFF5003 at 103.039 kb on - strand, within GFF5003 at 103.043 kb on - strand, within GFF5003 at 103.092 kb on + strand, within GFF5003 at 103.093 kb on - strand, within GFF5003 at 103.093 kb on - strand, within GFF5003 at 103.278 kb on + strand, within GFF5003 at 103.278 kb on + strand, within GFF5003 at 103.279 kb on - strand, within GFF5003 at 103.279 kb on - strand, within GFF5003 at 103.305 kb on + strand, within GFF5003 at 103.305 kb on + strand, within GFF5003 at 103.407 kb on + strand, within GFF5003 at 103.408 kb on - strand, within GFF5003 at 103.491 kb on + strand, within GFF5003 at 103.492 kb on - strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.524 kb on + strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.525 kb on - strand, within GFF5003 at 103.791 kb on + strand at 103.792 kb on - strand at 103.792 kb on - strand at 103.792 kb on - strand at 103.924 kb on + strand at 103.924 kb on + strand at 103.924 kb on + strand at 103.925 kb on - strand at 103.925 kb on - strand at 103.925 kb on - strand at 103.925 kb on - strand at 103.925 kb on - strand at 103.925 kb on - strand at 104.005 kb on + strand, within GFF5004 at 104.005 kb on + strand, within GFF5004 at 104.005 kb on + strand, within GFF5004 at 104.006 kb on - strand, within GFF5004 at 104.036 kb on + strand, within GFF5004 at 104.152 kb on + strand, within GFF5004 at 104.152 kb on + strand, within GFF5004 at 104.153 kb on - strand, within GFF5004 at 104.153 kb on - strand, within GFF5004 at 104.153 kb on - strand, within GFF5004 at 104.153 kb on - strand, within GFF5004 at 104.155 kb on + strand, within GFF5004 at 104.155 kb on + strand, within GFF5004 at 104.155 kb on + strand, within GFF5004 at 104.156 kb on - strand, within GFF5004 at 104.156 kb on - strand, within GFF5004 at 104.156 kb on - strand, within GFF5004 at 104.653 kb on + strand, within GFF5004 at 104.653 kb on + strand, within GFF5004 at 104.653 kb on + strand, within GFF5004 at 104.653 kb on + strand, within GFF5004 at 104.653 kb on + strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004 at 104.654 kb on - strand, within GFF5004
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 101,794 + GFF5002 0.16 +0.2 101,794 + GFF5002 0.16 -1.1 101,794 + GFF5002 0.16 -0.2 101,794 + GFF5002 0.16 -0.9 101,794 + GFF5002 0.16 -1.6 101,794 + GFF5002 0.16 -0.7 101,794 + GFF5002 0.16 -1.4 101,794 + GFF5002 0.16 -0.0 101,794 + GFF5002 0.16 +1.3 101,794 + GFF5002 0.16 -0.8 101,795 - GFF5002 0.16 -1.6 101,795 - GFF5002 0.16 -0.4 101,795 - GFF5002 0.16 +0.1 101,795 - GFF5002 0.16 -0.7 101,795 - GFF5002 0.16 -0.3 101,795 - GFF5002 0.16 -0.4 101,795 - GFF5002 0.16 -0.5 101,795 - GFF5002 0.16 -0.7 101,795 - GFF5002 0.16 -0.1 101,795 - GFF5002 0.16 +0.2 101,795 - GFF5002 0.16 +0.2 101,795 - GFF5002 0.16 +1.0 101,795 - GFF5002 0.16 -0.5 101,795 - GFF5002 0.16 -0.6 101,795 - GFF5002 0.16 +0.9 101,848 + GFF5002 0.21 -0.7 101,849 - GFF5002 0.21 -0.1 101,849 - GFF5002 0.21 -0.9 101,917 + GFF5002 0.27 -1.0 101,917 + GFF5002 0.27 +0.1 101,917 + GFF5002 0.27 +1.1 101,918 - GFF5002 0.27 -0.1 101,918 - GFF5002 0.27 -1.3 101,918 - GFF5002 0.27 -0.3 101,918 - GFF5002 0.27 +1.4 101,918 - GFF5002 0.27 +0.4 102,052 + GFF5002 0.39 -0.1 102,052 + GFF5002 0.39 -0.6 102,052 + GFF5002 0.39 -1.4 102,052 + GFF5002 0.39 +0.0 102,053 - GFF5002 0.39 +0.1 102,053 - GFF5002 0.39 -0.4 102,136 + GFF5002 0.46 +0.5 102,136 + GFF5002 0.46 +0.9 102,137 - GFF5002 0.47 +0.7 102,295 + GFF5002 0.60 -0.2 102,295 + GFF5002 0.60 -0.2 102,295 + GFF5002 0.60 -1.1 102,295 + GFF5002 0.60 -0.2 102,295 + GFF5002 0.60 +0.9 102,296 - GFF5002 0.61 -1.0 102,296 - GFF5002 0.61 -0.8 102,296 - GFF5002 0.61 -0.1 102,296 - GFF5002 0.61 -0.4 102,296 - GFF5002 0.61 +0.4 102,296 - GFF5002 0.61 -0.8 102,296 - GFF5002 0.61 +0.1 102,296 - GFF5002 0.61 +0.4 102,296 - GFF5002 0.61 -0.4 102,296 - GFF5002 0.61 -1.3 102,341 - GFF5002 0.65 -0.5 102,448 + GFF5002 0.74 -1.3 102,448 + GFF5002 0.74 +0.6 102,448 + GFF5002 0.74 +0.3 102,448 + GFF5002 0.74 -1.1 102,448 + GFF5002 0.74 +0.1 102,449 - GFF5002 0.74 -1.6 102,449 - GFF5002 0.74 -1.7 102,449 - GFF5002 0.74 +0.8 102,449 - GFF5002 0.74 -0.4 102,449 - GFF5002 0.74 -0.2 102,449 - GFF5002 0.74 +1.4 102,449 - GFF5002 0.74 -1.1 102,449 - GFF5002 0.74 +1.3 102,449 - GFF5002 0.74 -0.3 102,541 + GFF5002 0.82 -0.0 102,541 + GFF5002 0.82 +0.2 102,541 + GFF5002 0.82 +0.7 102,541 + GFF5002 0.82 -0.5 102,541 + GFF5002 0.82 +0.5 102,542 - GFF5002 0.82 -0.2 102,542 - GFF5002 0.82 -0.9 102,542 - GFF5002 0.82 -0.7 102,542 - GFF5002 0.82 +0.2 102,542 - GFF5002 0.82 +0.8 102,814 + +0.7 102,815 - -0.4 102,816 + -0.1 102,816 + -0.2 102,816 + -0.6 102,816 + -0.6 102,816 + +0.2 102,816 + +1.1 102,816 + -1.9 102,816 + +0.0 102,816 + -0.4 102,816 + -0.4 102,816 + -0.4 102,816 + +0.5 102,816 + -0.6 102,816 + +0.8 102,817 - -0.3 102,817 - -0.2 102,817 - +0.7 102,817 - +0.9 102,817 - +0.1 102,817 - +2.6 102,817 - +0.1 102,817 - -0.3 102,817 - -0.8 102,817 - -0.2 102,817 - +0.2 102,817 - -0.0 102,817 - +0.3 102,939 + GFF5003 0.19 -0.4 102,939 + GFF5003 0.19 -0.6 102,940 - GFF5003 0.19 -1.7 102,940 - GFF5003 0.19 +0.1 102,960 + GFF5003 0.21 -0.8 102,960 + GFF5003 0.21 -1.0 102,961 - GFF5003 0.21 -0.2 102,961 - GFF5003 0.21 -0.8 102,961 - GFF5003 0.21 +0.2 102,984 + GFF5003 0.23 -0.2 102,984 + GFF5003 0.23 -1.8 102,984 + GFF5003 0.23 -0.1 102,984 + GFF5003 0.23 -0.7 102,984 + GFF5003 0.23 -0.0 102,984 + GFF5003 0.23 -0.2 102,984 + GFF5003 0.23 -0.6 102,984 + GFF5003 0.23 +0.4 102,984 + GFF5003 0.23 +0.1 102,984 + GFF5003 0.23 -0.1 102,984 + GFF5003 0.23 -0.6 102,984 + GFF5003 0.23 +0.1 102,985 - GFF5003 0.23 +0.6 102,985 - GFF5003 0.23 +0.2 102,985 - GFF5003 0.23 -0.5 102,985 - GFF5003 0.23 -2.0 102,985 - GFF5003 0.23 -0.6 102,985 - GFF5003 0.23 +1.3 102,985 - GFF5003 0.23 -0.2 102,985 - GFF5003 0.23 -1.6 102,985 - GFF5003 0.23 -0.2 103,038 + GFF5003 0.28 -0.4 103,038 + GFF5003 0.28 +0.6 103,038 + GFF5003 0.28 -0.4 103,039 - GFF5003 0.28 -0.9 103,043 - GFF5003 0.28 -0.2 103,092 + GFF5003 0.33 +0.9 103,093 - GFF5003 0.33 -1.0 103,093 - GFF5003 0.33 -0.3 103,278 + GFF5003 0.50 +0.4 103,278 + GFF5003 0.50 +0.9 103,279 - GFF5003 0.50 +0.5 103,279 - GFF5003 0.50 +0.6 103,305 + GFF5003 0.52 -0.2 103,305 + GFF5003 0.52 -3.0 103,407 + GFF5003 0.62 -0.9 103,408 - GFF5003 0.62 +0.7 103,491 + GFF5003 0.69 -0.3 103,492 - GFF5003 0.69 -1.9 103,524 + GFF5003 0.72 -2.5 103,524 + GFF5003 0.72 -0.7 103,524 + GFF5003 0.72 -1.8 103,524 + GFF5003 0.72 -0.3 103,524 + GFF5003 0.72 -2.3 103,524 + GFF5003 0.72 -0.7 103,524 + GFF5003 0.72 -0.3 103,524 + GFF5003 0.72 -1.2 103,524 + GFF5003 0.72 -0.2 103,524 + GFF5003 0.72 -0.7 103,524 + GFF5003 0.72 -1.1 103,524 + GFF5003 0.72 +0.0 103,524 + GFF5003 0.72 +0.5 103,524 + GFF5003 0.72 -0.2 103,524 + GFF5003 0.72 +0.6 103,524 + GFF5003 0.72 -1.6 103,525 - GFF5003 0.73 +0.9 103,525 - GFF5003 0.73 -1.2 103,525 - GFF5003 0.73 +0.0 103,525 - GFF5003 0.73 -1.1 103,525 - GFF5003 0.73 -0.2 103,525 - GFF5003 0.73 -1.3 103,525 - GFF5003 0.73 -0.9 103,525 - GFF5003 0.73 -0.7 103,525 - GFF5003 0.73 -0.9 103,525 - GFF5003 0.73 -0.5 103,525 - GFF5003 0.73 +1.0 103,791 + -0.9 103,792 - +0.7 103,792 - +0.6 103,792 - +0.6 103,924 + -0.8 103,924 + +0.9 103,924 + +0.4 103,925 - +1.6 103,925 - -0.1 103,925 - -0.5 103,925 - +0.6 103,925 - -0.2 103,925 - +0.3 104,005 + GFF5004 0.14 -2.1 104,005 + GFF5004 0.14 -0.8 104,005 + GFF5004 0.14 +0.7 104,006 - GFF5004 0.14 -0.2 104,036 + GFF5004 0.17 -0.1 104,152 + GFF5004 0.27 -1.4 104,152 + GFF5004 0.27 +1.3 104,153 - GFF5004 0.27 +1.3 104,153 - GFF5004 0.27 -1.6 104,153 - GFF5004 0.27 -1.3 104,153 - GFF5004 0.27 -0.0 104,155 + GFF5004 0.27 -0.5 104,155 + GFF5004 0.27 +0.7 104,155 + GFF5004 0.27 -0.1 104,156 - GFF5004 0.27 -0.8 104,156 - GFF5004 0.27 -0.9 104,156 - GFF5004 0.27 -1.1 104,653 + GFF5004 0.68 -0.7 104,653 + GFF5004 0.68 -0.6 104,653 + GFF5004 0.68 -0.1 104,653 + GFF5004 0.68 +0.2 104,653 + GFF5004 0.68 -0.2 104,654 - GFF5004 0.68 -0.4 104,654 - GFF5004 0.68 +0.6 104,654 - GFF5004 0.68 -0.3 104,654 - GFF5004 0.68 +0.3 104,654 - GFF5004 0.68 -0.5 104,654 - GFF5004 0.68 -0.6 104,654 - GFF5004 0.68 +0.3 104,654 - GFF5004 0.68 -0.5 104,654 - GFF5004 0.68 -0.3 104,654 - GFF5004 0.68 +1.1 104,654 - GFF5004 0.68 -0.0 104,654 - GFF5004 0.68 +0.6 104,654 - GFF5004 0.68 -0.4
Or see this region's nucleotide sequence