Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4803 and GFF4804 overlap by 4 nucleotides GFF4804 and GFF4805 are separated by 45 nucleotides GFF4805 and GFF4806 overlap by 4 nucleotides GFF4806 and GFF4807 are separated by 27 nucleotides
GFF4803 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 173,339 to 174,829
GFF4803
GFF4804 - tRNA threonylcarbamoyladenosine biosynthesis protein TsaE, at 174,826 to 175,383
GFF4804
GFF4805 - Epoxyqueuosine reductase (EC 1.17.99.6) QueG, at 175,429 to 176,391
GFF4805
GFF4806 - Arsenate reductase (EC 1.20.4.1) glutaredoxin-coupled, glutaredoxin-like family, at 176,388 to 176,789
GFF4806
GFF4807 - Uncharacterized GST-like protein yncG, at 176,817 to 177,503
GFF4807
Position (kb)
175
176
177 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 174.441 kb on + strand, within GFF4803 at 174.441 kb on + strand, within GFF4803 at 174.442 kb on - strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.552 kb on + strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.553 kb on - strand, within GFF4803 at 174.554 kb on - strand, within GFF4803 at 174.702 kb on + strand at 174.702 kb on + strand at 174.702 kb on + strand at 174.702 kb on + strand at 174.703 kb on - strand at 174.703 kb on - strand at 174.703 kb on - strand at 174.703 kb on - strand at 175.306 kb on + strand, within GFF4804 at 175.306 kb on + strand, within GFF4804 at 175.306 kb on + strand, within GFF4804 at 175.348 kb on - strand at 175.348 kb on - strand at 175.407 kb on - strand at 175.407 kb on - strand at 175.407 kb on - strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.468 kb on + strand at 175.469 kb on - strand at 175.469 kb on - strand at 175.469 kb on - strand at 175.552 kb on + strand, within GFF4805 at 175.552 kb on + strand, within GFF4805 at 175.552 kb on + strand, within GFF4805 at 175.552 kb on + strand, within GFF4805 at 175.552 kb on + strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.553 kb on - strand, within GFF4805 at 175.636 kb on + strand, within GFF4805 at 175.636 kb on + strand, within GFF4805 at 175.636 kb on + strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.637 kb on - strand, within GFF4805 at 175.654 kb on + strand, within GFF4805 at 175.654 kb on + strand, within GFF4805 at 175.654 kb on + strand, within GFF4805 at 175.654 kb on + strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.655 kb on - strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.858 kb on + strand, within GFF4805 at 175.859 kb on - strand, within GFF4805 at 175.859 kb on - strand, within GFF4805 at 175.988 kb on - strand, within GFF4805 at 175.990 kb on + strand, within GFF4805 at 175.991 kb on - strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.056 kb on + strand, within GFF4805 at 176.057 kb on - strand, within GFF4805 at 176.057 kb on - strand, within GFF4805 at 176.057 kb on - strand, within GFF4805 at 176.058 kb on - strand, within GFF4805 at 176.389 kb on + strand at 176.389 kb on + strand at 176.389 kb on + strand at 176.390 kb on - strand at 176.390 kb on - strand at 176.390 kb on - strand at 176.390 kb on - strand at 176.390 kb on - strand at 176.569 kb on + strand, within GFF4806 at 176.569 kb on + strand, within GFF4806 at 176.570 kb on - strand, within GFF4806 at 176.570 kb on - strand, within GFF4806 at 176.665 kb on + strand, within GFF4806 at 176.746 kb on + strand, within GFF4806 at 176.746 kb on + strand, within GFF4806 at 176.747 kb on - strand, within GFF4806 at 176.747 kb on - strand, within GFF4806 at 176.747 kb on - strand, within GFF4806 at 176.800 kb on - strand at 176.818 kb on + strand at 176.819 kb on - strand at 176.819 kb on - strand at 176.819 kb on - strand at 176.819 kb on - strand at 176.935 kb on + strand, within GFF4807 at 176.980 kb on + strand, within GFF4807 at 176.980 kb on + strand, within GFF4807 at 176.980 kb on + strand, within GFF4807 at 176.981 kb on - strand, within GFF4807 at 176.981 kb on - strand, within GFF4807 at 176.981 kb on - strand, within GFF4807 at 176.981 kb on - strand, within GFF4807 at 177.016 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.157 kb on + strand, within GFF4807 at 177.158 kb on - strand, within GFF4807 at 177.158 kb on - strand, within GFF4807 at 177.175 kb on + strand, within GFF4807 at 177.175 kb on + strand, within GFF4807 at 177.194 kb on - strand, within GFF4807 at 177.309 kb on + strand, within GFF4807 at 177.309 kb on + strand, within GFF4807 at 177.310 kb on - strand, within GFF4807
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 174,441 + GFF4803 0.74 +0.2 174,441 + GFF4803 0.74 -0.2 174,442 - GFF4803 0.74 +0.4 174,552 + GFF4803 0.81 -0.2 174,552 + GFF4803 0.81 -0.0 174,552 + GFF4803 0.81 +0.4 174,552 + GFF4803 0.81 +0.8 174,552 + GFF4803 0.81 +0.4 174,552 + GFF4803 0.81 -0.5 174,553 - GFF4803 0.81 +1.6 174,553 - GFF4803 0.81 -0.5 174,553 - GFF4803 0.81 +0.9 174,553 - GFF4803 0.81 +0.4 174,553 - GFF4803 0.81 +1.2 174,553 - GFF4803 0.81 +0.0 174,553 - GFF4803 0.81 -0.6 174,554 - GFF4803 0.81 -0.7 174,702 + -1.2 174,702 + -0.4 174,702 + -1.1 174,702 + +0.7 174,703 - +0.1 174,703 - +0.5 174,703 - -1.9 174,703 - +0.2 175,306 + GFF4804 0.86 -0.3 175,306 + GFF4804 0.86 +1.0 175,306 + GFF4804 0.86 +0.0 175,348 - +0.7 175,348 - +0.7 175,407 - -0.2 175,407 - -2.2 175,407 - +1.3 175,468 + +0.2 175,468 + -0.6 175,468 + -0.4 175,468 + +0.3 175,468 + -0.1 175,468 + +0.2 175,468 + -0.4 175,468 + -1.2 175,468 + -0.3 175,468 + +0.1 175,468 + -0.4 175,468 + -0.4 175,468 + +0.2 175,468 + -2.0 175,468 + -0.1 175,469 - -0.1 175,469 - +0.2 175,469 - -0.0 175,552 + GFF4805 0.13 -0.9 175,552 + GFF4805 0.13 +0.2 175,552 + GFF4805 0.13 -0.4 175,552 + GFF4805 0.13 -0.7 175,552 + GFF4805 0.13 +0.4 175,553 - GFF4805 0.13 -0.8 175,553 - GFF4805 0.13 -0.6 175,553 - GFF4805 0.13 +0.5 175,553 - GFF4805 0.13 +0.7 175,553 - GFF4805 0.13 -0.9 175,553 - GFF4805 0.13 -0.3 175,636 + GFF4805 0.21 +0.6 175,636 + GFF4805 0.21 +1.1 175,636 + GFF4805 0.21 -0.9 175,637 - GFF4805 0.22 -0.6 175,637 - GFF4805 0.22 +0.1 175,637 - GFF4805 0.22 +0.5 175,637 - GFF4805 0.22 -0.9 175,637 - GFF4805 0.22 -1.5 175,637 - GFF4805 0.22 -0.0 175,637 - GFF4805 0.22 +1.1 175,637 - GFF4805 0.22 -0.7 175,637 - GFF4805 0.22 -0.5 175,637 - GFF4805 0.22 -0.4 175,637 - GFF4805 0.22 -1.1 175,637 - GFF4805 0.22 +2.0 175,637 - GFF4805 0.22 -1.3 175,654 + GFF4805 0.23 +0.2 175,654 + GFF4805 0.23 +0.2 175,654 + GFF4805 0.23 -0.2 175,654 + GFF4805 0.23 -0.2 175,655 - GFF4805 0.23 -0.1 175,655 - GFF4805 0.23 -0.4 175,655 - GFF4805 0.23 -0.9 175,655 - GFF4805 0.23 +0.8 175,655 - GFF4805 0.23 +0.1 175,655 - GFF4805 0.23 -0.6 175,858 + GFF4805 0.45 -0.2 175,858 + GFF4805 0.45 -2.0 175,858 + GFF4805 0.45 -2.2 175,858 + GFF4805 0.45 +0.3 175,858 + GFF4805 0.45 -1.0 175,858 + GFF4805 0.45 -0.6 175,858 + GFF4805 0.45 -0.6 175,859 - GFF4805 0.45 -0.2 175,859 - GFF4805 0.45 -0.1 175,988 - GFF4805 0.58 -0.8 175,990 + GFF4805 0.58 -0.4 175,991 - GFF4805 0.58 -0.9 176,056 + GFF4805 0.65 -0.6 176,056 + GFF4805 0.65 +0.1 176,056 + GFF4805 0.65 -0.5 176,056 + GFF4805 0.65 -0.8 176,056 + GFF4805 0.65 -0.0 176,057 - GFF4805 0.65 -0.1 176,057 - GFF4805 0.65 +0.1 176,057 - GFF4805 0.65 -0.5 176,058 - GFF4805 0.65 +0.4 176,389 + -0.8 176,389 + +2.0 176,389 + -0.4 176,390 - -0.8 176,390 - +0.1 176,390 - -1.2 176,390 - -0.0 176,390 - -0.8 176,569 + GFF4806 0.45 +0.7 176,569 + GFF4806 0.45 +0.1 176,570 - GFF4806 0.45 -0.9 176,570 - GFF4806 0.45 -0.4 176,665 + GFF4806 0.69 -0.6 176,746 + GFF4806 0.89 +0.0 176,746 + GFF4806 0.89 -0.8 176,747 - GFF4806 0.89 +0.1 176,747 - GFF4806 0.89 -1.3 176,747 - GFF4806 0.89 -1.8 176,800 - -1.4 176,818 + +0.6 176,819 - -0.4 176,819 - -1.5 176,819 - -0.2 176,819 - +1.2 176,935 + GFF4807 0.17 -0.3 176,980 + GFF4807 0.24 -0.1 176,980 + GFF4807 0.24 -0.8 176,980 + GFF4807 0.24 +0.2 176,981 - GFF4807 0.24 +0.9 176,981 - GFF4807 0.24 -0.1 176,981 - GFF4807 0.24 -1.3 176,981 - GFF4807 0.24 +0.4 177,016 + GFF4807 0.29 -0.8 177,157 + GFF4807 0.49 -0.4 177,157 + GFF4807 0.49 -0.4 177,157 + GFF4807 0.49 +0.1 177,157 + GFF4807 0.49 +0.6 177,157 + GFF4807 0.49 -0.2 177,158 - GFF4807 0.50 -0.4 177,158 - GFF4807 0.50 -1.1 177,175 + GFF4807 0.52 +0.7 177,175 + GFF4807 0.52 -0.8 177,194 - GFF4807 0.55 -0.0 177,309 + GFF4807 0.72 -1.1 177,309 + GFF4807 0.72 +0.3 177,310 - GFF4807 0.72 +0.2
Or see this region's nucleotide sequence