Strain Fitness in Variovorax sp. SCN45 around GFF4544

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4542 and GFF4543 overlap by 4 nucleotidesGFF4543 and GFF4544 overlap by 4 nucleotidesGFF4544 and GFF4545 are separated by 161 nucleotides GFF4542 - hypothetical protein, at 180,870 to 181,364 GFF4542 GFF4543 - no description, at 181,361 to 181,795 GFF4543 GFF4544 - RNA polymerase sigma factor RpoE, at 181,792 to 182,292 GFF4544 GFF4545 - Transcriptional regulator, AraC family, at 182,454 to 183,251 GFF4545 Position (kb) 181 182 183Strain fitness (log2 ratio) -2 -1 0 1 2 3at 180.871 kb on + strandat 180.871 kb on + strandat 180.872 kb on - strandat 180.872 kb on - strandat 181.384 kb on - strandat 181.758 kb on + strandat 181.758 kb on + strandat 181.759 kb on - strandat 181.759 kb on - strandat 181.759 kb on - strandat 181.803 kb on - strandat 181.810 kb on + strandat 181.810 kb on + strandat 181.810 kb on + strandat 181.810 kb on + strandat 181.810 kb on + strandat 181.810 kb on + strandat 181.811 kb on - strandat 181.811 kb on - strandat 181.811 kb on - strandat 181.811 kb on - strandat 181.925 kb on + strand, within GFF4544at 181.925 kb on + strand, within GFF4544at 181.925 kb on + strand, within GFF4544at 181.925 kb on + strand, within GFF4544at 181.925 kb on + strand, within GFF4544at 181.925 kb on + strand, within GFF4544at 181.926 kb on - strand, within GFF4544at 181.926 kb on - strand, within GFF4544at 181.926 kb on - strand, within GFF4544at 181.926 kb on - strand, within GFF4544at 181.928 kb on + strand, within GFF4544at 181.928 kb on + strand, within GFF4544at 181.928 kb on + strand, within GFF4544at 181.928 kb on + strand, within GFF4544at 181.928 kb on + strand, within GFF4544at 181.928 kb on + strand, within GFF4544at 181.928 kb on + strand, within GFF4544at 181.929 kb on - strand, within GFF4544at 181.929 kb on - strand, within GFF4544at 181.929 kb on - strand, within GFF4544at 181.929 kb on - strand, within GFF4544at 181.929 kb on - strand, within GFF4544at 181.929 kb on - strand, within GFF4544at 182.102 kb on + strand, within GFF4544at 182.103 kb on - strand, within GFF4544at 182.103 kb on - strand, within GFF4544at 182.103 kb on - strand, within GFF4544at 182.327 kb on - strandat 182.408 kb on + strandat 182.408 kb on + strandat 182.408 kb on + strandat 182.485 kb on + strandat 182.548 kb on + strand, within GFF4545at 182.548 kb on + strand, within GFF4545at 182.549 kb on - strand, within GFF4545at 182.549 kb on - strand, within GFF4545at 182.797 kb on + strand, within GFF4545at 182.797 kb on + strand, within GFF4545at 182.797 kb on + strand, within GFF4545at 182.798 kb on - strand, within GFF4545at 182.983 kb on + strand, within GFF4545at 182.983 kb on + strand, within GFF4545at 182.983 kb on + strand, within GFF4545at 182.983 kb on + strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.984 kb on - strand, within GFF4545at 182.995 kb on + strand, within GFF4545at 182.996 kb on - strand, within GFF4545at 182.996 kb on - strand, within GFF4545at 183.055 kb on + strand, within GFF4545at 183.055 kb on + strand, within GFF4545at 183.056 kb on - strand, within GFF4545at 183.056 kb on - strand, within GFF4545at 183.118 kb on + strand, within GFF4545at 183.118 kb on + strand, within GFF4545at 183.118 kb on + strand, within GFF4545at 183.118 kb on + strand, within GFF4545at 183.118 kb on + strand, within GFF4545at 183.118 kb on + strand, within GFF4545at 183.119 kb on - strand, within GFF4545at 183.119 kb on - strand, within GFF4545at 183.119 kb on - strand, within GFF4545at 183.119 kb on - strand, within GFF4545at 183.190 kb on + strandat 183.190 kb on + strandat 183.190 kb on + strandat 183.190 kb on + strandat 183.191 kb on - strandat 183.191 kb on - strandat 183.191 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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180,871 + -0.6
180,871 + +1.5
180,872 - +0.2
180,872 - -0.1
181,384 - -0.2
181,758 + -0.8
181,758 + -2.0
181,759 - -0.1
181,759 - -1.3
181,759 - -0.3
181,803 - -0.2
181,810 + +0.2
181,810 + +1.8
181,810 + -0.4
181,810 + -0.3
181,810 + -0.1
181,810 + -1.5
181,811 - +0.3
181,811 - +0.8
181,811 - -1.5
181,811 - +0.4
181,925 + GFF4544 0.27 +0.7
181,925 + GFF4544 0.27 +0.2
181,925 + GFF4544 0.27 -0.9
181,925 + GFF4544 0.27 +0.1
181,925 + GFF4544 0.27 +0.4
181,925 + GFF4544 0.27 +0.3
181,926 - GFF4544 0.27 -0.1
181,926 - GFF4544 0.27 -0.4
181,926 - GFF4544 0.27 -0.6
181,926 - GFF4544 0.27 +0.6
181,928 + GFF4544 0.27 +0.8
181,928 + GFF4544 0.27 -0.6
181,928 + GFF4544 0.27 +1.3
181,928 + GFF4544 0.27 -0.3
181,928 + GFF4544 0.27 +0.6
181,928 + GFF4544 0.27 -0.1
181,928 + GFF4544 0.27 -0.3
181,929 - GFF4544 0.27 +1.1
181,929 - GFF4544 0.27 -0.7
181,929 - GFF4544 0.27 +1.1
181,929 - GFF4544 0.27 +0.4
181,929 - GFF4544 0.27 -1.0
181,929 - GFF4544 0.27 -0.8
182,102 + GFF4544 0.62 -0.3
182,103 - GFF4544 0.62 +0.1
182,103 - GFF4544 0.62 +1.0
182,103 - GFF4544 0.62 -1.6
182,327 - -0.4
182,408 + -1.2
182,408 + -0.3
182,408 + -1.6
182,485 + -0.3
182,548 + GFF4545 0.12 -0.1
182,548 + GFF4545 0.12 +0.0
182,549 - GFF4545 0.12 -1.7
182,549 - GFF4545 0.12 -0.1
182,797 + GFF4545 0.43 -0.1
182,797 + GFF4545 0.43 -0.3
182,797 + GFF4545 0.43 -0.2
182,798 - GFF4545 0.43 -0.4
182,983 + GFF4545 0.66 +0.2
182,983 + GFF4545 0.66 +0.3
182,983 + GFF4545 0.66 +0.5
182,983 + GFF4545 0.66 -0.3
182,984 - GFF4545 0.66 -0.2
182,984 - GFF4545 0.66 +0.6
182,984 - GFF4545 0.66 +0.6
182,984 - GFF4545 0.66 +1.8
182,984 - GFF4545 0.66 -1.1
182,984 - GFF4545 0.66 -0.0
182,984 - GFF4545 0.66 +0.8
182,984 - GFF4545 0.66 -1.1
182,984 - GFF4545 0.66 -0.6
182,995 + GFF4545 0.68 -0.6
182,996 - GFF4545 0.68 -0.2
182,996 - GFF4545 0.68 +0.6
183,055 + GFF4545 0.75 +1.0
183,055 + GFF4545 0.75 +1.2
183,056 - GFF4545 0.75 -0.3
183,056 - GFF4545 0.75 +0.4
183,118 + GFF4545 0.83 +0.4
183,118 + GFF4545 0.83 -0.8
183,118 + GFF4545 0.83 -0.8
183,118 + GFF4545 0.83 +1.5
183,118 + GFF4545 0.83 -0.5
183,118 + GFF4545 0.83 +1.8
183,119 - GFF4545 0.83 +3.0
183,119 - GFF4545 0.83 -0.3
183,119 - GFF4545 0.83 +0.1
183,119 - GFF4545 0.83 +0.2
183,190 + -0.6
183,190 + +1.4
183,190 + -0.6
183,190 + -2.0
183,191 - +0.2
183,191 - -1.9
183,191 - -0.3

Or see this region's nucleotide sequence