Strain Fitness in Variovorax sp. SCN45 around GFF4329

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4328 and GFF4329 are separated by 151 nucleotidesGFF4329 and GFF4330 are separated by 3 nucleotides GFF4328 - hypothetical protein, at 249,979 to 251,094 GFF4328 GFF4329 - Enoyl-CoA hydratase (EC 4.2.1.17), at 251,246 to 252,037 GFF4329 GFF4330 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 252,041 to 252,949 GFF4330 Position (kb) 251 252 253Strain fitness (log2 ratio) -2 -1 0 1 2 3at 250.416 kb on - strand, within GFF4328at 250.508 kb on + strand, within GFF4328at 251.116 kb on + strandat 251.116 kb on + strandat 251.117 kb on - strandat 251.131 kb on + strandat 251.144 kb on + strandat 251.183 kb on - strandat 251.459 kb on + strand, within GFF4329at 251.459 kb on + strand, within GFF4329at 251.460 kb on - strand, within GFF4329at 252.913 kb on + strandat 252.913 kb on + strandat 252.947 kb on + strandat 253.033 kb on + strandat 253.034 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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250,416 - GFF4328 0.39 -1.3
250,508 + GFF4328 0.47 -1.3
251,116 + -1.1
251,116 + -0.1
251,117 - -1.7
251,131 + -1.6
251,144 + +0.6
251,183 - -1.0
251,459 + GFF4329 0.27 +1.5
251,459 + GFF4329 0.27 -0.6
251,460 - GFF4329 0.27 +0.3
252,913 + +0.5
252,913 + -1.2
252,947 + +3.0
253,033 + -2.1
253,034 - -0.0

Or see this region's nucleotide sequence