Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4009 and GFF4010 are separated by 214 nucleotides GFF4010 and GFF4011 overlap by 4 nucleotides
GFF4009 - D-amino acid dehydrogenase (EC 1.4.99.6), at 216,917 to 218,128
GFF4009
GFF4010 - no description, at 218,343 to 219,683
GFF4010
GFF4011 - no description, at 219,680 to 220,813
GFF4011
Position (kb) 218
219
220 Strain fitness (log2 ratio) -2
-1
0
1
2 at 217.560 kb on + strand, within GFF4009 at 217.561 kb on - strand, within GFF4009 at 217.710 kb on + strand, within GFF4009 at 217.710 kb on + strand, within GFF4009 at 217.710 kb on + strand, within GFF4009 at 217.710 kb on + strand, within GFF4009 at 217.710 kb on + strand, within GFF4009 at 217.710 kb on + strand, within GFF4009 at 217.710 kb on + strand, within GFF4009 at 217.710 kb on + strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.711 kb on - strand, within GFF4009 at 217.980 kb on + strand, within GFF4009 at 217.980 kb on + strand, within GFF4009 at 217.980 kb on + strand, within GFF4009 at 217.980 kb on + strand, within GFF4009 at 217.980 kb on + strand, within GFF4009 at 217.980 kb on + strand, within GFF4009 at 217.980 kb on + strand, within GFF4009 at 217.980 kb on + strand, within GFF4009 at 217.981 kb on - strand, within GFF4009 at 217.981 kb on - strand, within GFF4009 at 217.981 kb on - strand, within GFF4009 at 217.986 kb on + strand, within GFF4009 at 217.986 kb on + strand, within GFF4009 at 217.986 kb on + strand, within GFF4009 at 217.986 kb on + strand, within GFF4009 at 217.986 kb on + strand, within GFF4009 at 217.986 kb on + strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 217.987 kb on - strand, within GFF4009 at 218.186 kb on + strand at 218.187 kb on - strand at 218.187 kb on - strand at 218.187 kb on - strand at 218.212 kb on + strand at 218.213 kb on - strand at 218.213 kb on - strand at 218.235 kb on + strand at 218.236 kb on - strand at 218.244 kb on + strand at 218.244 kb on + strand at 218.244 kb on + strand at 218.244 kb on + strand at 218.244 kb on + strand at 218.244 kb on + strand at 218.244 kb on + strand at 218.244 kb on + strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.245 kb on - strand at 218.263 kb on + strand at 218.263 kb on + strand at 218.263 kb on + strand at 218.263 kb on + strand at 218.264 kb on - strand at 218.264 kb on - strand at 218.264 kb on - strand at 218.264 kb on - strand at 218.270 kb on + strand at 218.270 kb on + strand at 218.344 kb on + strand at 218.344 kb on + strand at 218.344 kb on + strand at 218.345 kb on - strand at 218.345 kb on - strand at 218.345 kb on - strand at 218.345 kb on - strand at 218.345 kb on - strand at 218.466 kb on - strand at 218.466 kb on - strand at 218.682 kb on + strand, within GFF4010 at 218.682 kb on + strand, within GFF4010 at 218.683 kb on - strand, within GFF4010 at 218.683 kb on - strand, within GFF4010 at 218.735 kb on + strand, within GFF4010 at 218.736 kb on - strand, within GFF4010 at 218.736 kb on - strand, within GFF4010 at 218.958 kb on + strand, within GFF4010 at 218.959 kb on - strand, within GFF4010 at 218.959 kb on - strand, within GFF4010 at 218.959 kb on - strand, within GFF4010 at 219.176 kb on + strand, within GFF4010 at 219.176 kb on + strand, within GFF4010 at 219.176 kb on + strand, within GFF4010 at 219.176 kb on + strand, within GFF4010 at 219.177 kb on - strand, within GFF4010 at 219.177 kb on - strand, within GFF4010 at 219.177 kb on - strand, within GFF4010 at 219.177 kb on - strand, within GFF4010 at 219.213 kb on + strand, within GFF4010 at 219.213 kb on + strand, within GFF4010 at 219.213 kb on + strand, within GFF4010 at 219.214 kb on - strand, within GFF4010 at 219.214 kb on - strand, within GFF4010 at 219.214 kb on - strand, within GFF4010 at 219.214 kb on - strand, within GFF4010 at 219.214 kb on - strand, within GFF4010 at 219.222 kb on + strand, within GFF4010 at 219.222 kb on + strand, within GFF4010 at 219.222 kb on + strand, within GFF4010 at 219.223 kb on - strand, within GFF4010 at 219.223 kb on - strand, within GFF4010 at 219.223 kb on - strand, within GFF4010 at 219.223 kb on - strand, within GFF4010 at 219.223 kb on - strand, within GFF4010 at 219.234 kb on - strand, within GFF4010 at 219.292 kb on + strand, within GFF4010 at 219.293 kb on - strand, within GFF4010 at 219.429 kb on + strand, within GFF4010 at 219.429 kb on + strand, within GFF4010 at 219.438 kb on - strand, within GFF4010 at 219.438 kb on - strand, within GFF4010 at 219.450 kb on - strand, within GFF4010 at 219.456 kb on + strand, within GFF4010 at 219.457 kb on - strand, within GFF4010 at 219.482 kb on + strand, within GFF4010 at 219.482 kb on + strand, within GFF4010 at 219.483 kb on - strand, within GFF4010 at 219.483 kb on - strand, within GFF4010 at 219.483 kb on - strand, within GFF4010 at 219.483 kb on - strand, within GFF4010 at 219.483 kb on - strand, within GFF4010 at 219.484 kb on - strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.532 kb on + strand, within GFF4010 at 219.533 kb on - strand, within GFF4010 at 219.533 kb on - strand, within GFF4010 at 219.533 kb on - strand, within GFF4010 at 219.533 kb on - strand, within GFF4010 at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.579 kb on + strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.580 kb on - strand at 219.664 kb on + strand at 219.665 kb on - strand at 219.665 kb on - strand at 219.671 kb on + strand at 219.671 kb on + strand at 219.671 kb on + strand at 219.671 kb on + strand at 219.671 kb on + strand at 219.672 kb on - strand at 219.729 kb on - strand at 219.729 kb on - strand at 219.729 kb on - strand at 219.821 kb on + strand, within GFF4011 at 219.822 kb on - strand, within GFF4011 at 219.822 kb on - strand, within GFF4011 at 219.822 kb on - strand, within GFF4011 at 219.841 kb on + strand, within GFF4011 at 219.842 kb on - strand, within GFF4011 at 219.975 kb on + strand, within GFF4011 at 219.976 kb on - strand, within GFF4011 at 219.976 kb on - strand, within GFF4011 at 220.018 kb on + strand, within GFF4011 at 220.119 kb on + strand, within GFF4011 at 220.220 kb on + strand, within GFF4011 at 220.231 kb on - strand, within GFF4011 at 220.241 kb on + strand, within GFF4011 at 220.258 kb on + strand, within GFF4011 at 220.259 kb on - strand, within GFF4011 at 220.288 kb on + strand, within GFF4011 at 220.289 kb on - strand, within GFF4011 at 220.289 kb on - strand, within GFF4011 at 220.331 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.631 kb on + strand, within GFF4011 at 220.632 kb on - strand, within GFF4011 at 220.632 kb on - strand, within GFF4011 at 220.632 kb on - strand, within GFF4011 at 220.632 kb on - strand, within GFF4011 at 220.632 kb on - strand, within GFF4011 at 220.632 kb on - strand, within GFF4011 at 220.632 kb on - strand, within GFF4011 at 220.632 kb on - strand, within GFF4011 at 220.662 kb on + strand, within GFF4011 at 220.662 kb on + strand, within GFF4011 at 220.662 kb on + strand, within GFF4011
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 217,560 + GFF4009 0.53 +0.5 217,561 - GFF4009 0.53 -0.2 217,710 + GFF4009 0.65 +1.6 217,710 + GFF4009 0.65 -0.1 217,710 + GFF4009 0.65 -0.9 217,710 + GFF4009 0.65 -0.7 217,710 + GFF4009 0.65 -1.4 217,710 + GFF4009 0.65 +0.1 217,710 + GFF4009 0.65 -0.2 217,710 + GFF4009 0.65 -0.1 217,711 - GFF4009 0.66 -1.4 217,711 - GFF4009 0.66 +0.5 217,711 - GFF4009 0.66 +0.9 217,711 - GFF4009 0.66 -1.1 217,711 - GFF4009 0.66 +2.0 217,711 - GFF4009 0.66 -0.9 217,711 - GFF4009 0.66 -1.7 217,711 - GFF4009 0.66 +0.5 217,711 - GFF4009 0.66 -0.2 217,980 + GFF4009 0.88 -1.8 217,980 + GFF4009 0.88 -0.8 217,980 + GFF4009 0.88 -0.7 217,980 + GFF4009 0.88 -0.4 217,980 + GFF4009 0.88 -1.7 217,980 + GFF4009 0.88 -0.7 217,980 + GFF4009 0.88 -0.4 217,980 + GFF4009 0.88 -0.2 217,981 - GFF4009 0.88 -0.3 217,981 - GFF4009 0.88 +2.6 217,981 - GFF4009 0.88 +0.6 217,986 + GFF4009 0.88 -0.4 217,986 + GFF4009 0.88 +0.8 217,986 + GFF4009 0.88 +0.6 217,986 + GFF4009 0.88 -0.4 217,986 + GFF4009 0.88 -0.3 217,986 + GFF4009 0.88 -0.7 217,987 - GFF4009 0.88 -0.7 217,987 - GFF4009 0.88 -1.0 217,987 - GFF4009 0.88 -0.7 217,987 - GFF4009 0.88 -0.4 217,987 - GFF4009 0.88 -1.0 217,987 - GFF4009 0.88 -1.7 217,987 - GFF4009 0.88 +0.5 217,987 - GFF4009 0.88 -0.9 217,987 - GFF4009 0.88 +0.4 217,987 - GFF4009 0.88 +0.1 218,186 + -0.2 218,187 - -0.0 218,187 - -0.8 218,187 - +0.5 218,212 + -1.8 218,213 - +0.4 218,213 - -0.2 218,235 + -0.2 218,236 - -0.7 218,244 + -0.5 218,244 + -1.2 218,244 + -0.7 218,244 + -0.8 218,244 + +1.5 218,244 + +0.6 218,244 + -0.4 218,244 + +0.5 218,245 - +0.8 218,245 - -0.5 218,245 - -0.1 218,245 - +0.9 218,245 - -0.4 218,245 - +0.9 218,245 - -1.1 218,245 - -1.8 218,245 - -1.9 218,245 - +0.5 218,263 + -0.5 218,263 + +0.4 218,263 + +0.1 218,263 + +1.0 218,264 - -1.0 218,264 - +0.3 218,264 - +0.2 218,264 - -0.6 218,270 + +0.5 218,270 + -0.1 218,344 + -2.4 218,344 + -0.0 218,344 + -0.1 218,345 - -0.2 218,345 - -1.0 218,345 - -0.7 218,345 - -0.8 218,345 - -0.5 218,466 - +1.4 218,466 - -0.9 218,682 + GFF4010 0.25 -0.4 218,682 + GFF4010 0.25 -0.4 218,683 - GFF4010 0.25 +0.2 218,683 - GFF4010 0.25 +0.1 218,735 + GFF4010 0.29 -1.7 218,736 - GFF4010 0.29 +0.3 218,736 - GFF4010 0.29 +0.3 218,958 + GFF4010 0.46 +0.5 218,959 - GFF4010 0.46 +0.1 218,959 - GFF4010 0.46 -0.4 218,959 - GFF4010 0.46 -1.7 219,176 + GFF4010 0.62 -0.9 219,176 + GFF4010 0.62 +0.9 219,176 + GFF4010 0.62 +0.2 219,176 + GFF4010 0.62 +0.6 219,177 - GFF4010 0.62 -0.9 219,177 - GFF4010 0.62 -0.2 219,177 - GFF4010 0.62 +0.2 219,177 - GFF4010 0.62 +0.2 219,213 + GFF4010 0.65 +1.4 219,213 + GFF4010 0.65 +1.2 219,213 + GFF4010 0.65 +0.5 219,214 - GFF4010 0.65 +0.0 219,214 - GFF4010 0.65 -0.8 219,214 - GFF4010 0.65 -0.5 219,214 - GFF4010 0.65 +1.2 219,214 - GFF4010 0.65 -1.8 219,222 + GFF4010 0.66 +1.2 219,222 + GFF4010 0.66 +0.4 219,222 + GFF4010 0.66 -0.6 219,223 - GFF4010 0.66 -0.7 219,223 - GFF4010 0.66 +1.2 219,223 - GFF4010 0.66 -0.4 219,223 - GFF4010 0.66 +0.4 219,223 - GFF4010 0.66 -0.2 219,234 - GFF4010 0.66 +0.3 219,292 + GFF4010 0.71 -1.6 219,293 - GFF4010 0.71 -0.3 219,429 + GFF4010 0.81 +0.2 219,429 + GFF4010 0.81 +0.8 219,438 - GFF4010 0.82 +1.0 219,438 - GFF4010 0.82 -0.1 219,450 - GFF4010 0.83 -1.0 219,456 + GFF4010 0.83 +1.5 219,457 - GFF4010 0.83 -0.8 219,482 + GFF4010 0.85 -0.3 219,482 + GFF4010 0.85 +0.1 219,483 - GFF4010 0.85 -0.6 219,483 - GFF4010 0.85 -0.0 219,483 - GFF4010 0.85 -0.0 219,483 - GFF4010 0.85 -0.7 219,483 - GFF4010 0.85 -0.7 219,484 - GFF4010 0.85 -0.3 219,532 + GFF4010 0.89 +0.1 219,532 + GFF4010 0.89 -0.7 219,532 + GFF4010 0.89 +0.8 219,532 + GFF4010 0.89 +0.8 219,532 + GFF4010 0.89 +0.6 219,532 + GFF4010 0.89 +2.6 219,532 + GFF4010 0.89 +0.6 219,532 + GFF4010 0.89 +1.0 219,532 + GFF4010 0.89 +0.4 219,532 + GFF4010 0.89 -1.0 219,533 - GFF4010 0.89 -0.3 219,533 - GFF4010 0.89 -0.1 219,533 - GFF4010 0.89 -1.1 219,533 - GFF4010 0.89 -0.8 219,579 + -0.2 219,579 + -1.2 219,579 + -0.7 219,579 + -0.1 219,579 + -0.9 219,579 + -0.4 219,579 + -0.1 219,579 + +0.1 219,579 + -0.1 219,579 + -0.7 219,579 + -1.4 219,579 + -0.5 219,579 + +1.9 219,579 + +0.3 219,579 + -1.9 219,579 + -1.4 219,579 + +1.2 219,579 + +0.6 219,579 + +0.5 219,579 + -1.9 219,579 + -0.4 219,579 + +0.1 219,579 + -0.6 219,579 + +0.6 219,579 + +1.4 219,579 + +0.6 219,579 + +0.8 219,579 + -0.1 219,579 + +0.1 219,579 + +0.1 219,580 - -0.3 219,580 - +0.4 219,580 - +0.5 219,580 - +0.6 219,580 - +0.2 219,580 - -0.7 219,580 - -0.8 219,580 - +0.4 219,580 - +0.8 219,580 - +0.1 219,580 - -1.5 219,580 - +0.2 219,580 - -0.4 219,580 - +0.1 219,580 - +0.6 219,580 - -0.1 219,580 - -1.8 219,580 - -0.4 219,580 - +0.6 219,580 - +0.2 219,580 - +0.4 219,580 - -1.5 219,664 + +0.7 219,665 - -0.0 219,665 - -0.2 219,671 + -0.6 219,671 + +1.1 219,671 + +0.0 219,671 + -0.8 219,671 + -0.7 219,672 - +0.4 219,729 - -0.7 219,729 - -1.3 219,729 - -0.1 219,821 + GFF4011 0.12 +1.3 219,822 - GFF4011 0.13 -0.4 219,822 - GFF4011 0.13 +0.2 219,822 - GFF4011 0.13 +0.5 219,841 + GFF4011 0.14 +1.9 219,842 - GFF4011 0.14 -0.4 219,975 + GFF4011 0.26 -0.5 219,976 - GFF4011 0.26 +0.2 219,976 - GFF4011 0.26 +1.4 220,018 + GFF4011 0.30 +0.0 220,119 + GFF4011 0.39 +1.1 220,220 + GFF4011 0.48 +0.5 220,231 - GFF4011 0.49 +0.4 220,241 + GFF4011 0.49 +0.2 220,258 + GFF4011 0.51 -0.6 220,259 - GFF4011 0.51 -0.4 220,288 + GFF4011 0.54 -0.6 220,289 - GFF4011 0.54 +0.4 220,289 - GFF4011 0.54 +0.2 220,331 + GFF4011 0.57 -0.1 220,631 + GFF4011 0.84 -1.3 220,631 + GFF4011 0.84 -0.6 220,631 + GFF4011 0.84 -0.8 220,631 + GFF4011 0.84 +0.9 220,631 + GFF4011 0.84 +0.3 220,631 + GFF4011 0.84 +0.9 220,631 + GFF4011 0.84 -0.8 220,631 + GFF4011 0.84 +1.2 220,631 + GFF4011 0.84 -0.0 220,631 + GFF4011 0.84 +0.0 220,632 - GFF4011 0.84 +0.1 220,632 - GFF4011 0.84 -0.4 220,632 - GFF4011 0.84 +0.1 220,632 - GFF4011 0.84 +0.6 220,632 - GFF4011 0.84 -0.2 220,632 - GFF4011 0.84 +0.6 220,632 - GFF4011 0.84 -0.1 220,632 - GFF4011 0.84 +0.1 220,662 + GFF4011 0.87 +0.5 220,662 + GFF4011 0.87 +0.4 220,662 + GFF4011 0.87 -0.3
Or see this region's nucleotide sequence