Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3787 and GFF3788 are separated by 65 nucleotides GFF3788 and GFF3789 are separated by 121 nucleotides GFF3789 and GFF3790 are separated by 1 nucleotides
GFF3787 - Biotin carboxylase (EC 6.3.4.14), at 281,837 to 285,130
GFF3787
GFF3788 - putative secreted protein, at 285,196 to 286,521
GFF3788
GFF3789 - hypothetical protein, at 286,643 to 287,017
GFF3789
GFF3790 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 287,019 to 287,885
GFF3790
Position (kb)
285
286
287 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 284.333 kb on + strand, within GFF3787 at 284.333 kb on + strand, within GFF3787 at 284.333 kb on + strand, within GFF3787 at 284.334 kb on - strand, within GFF3787 at 284.334 kb on - strand, within GFF3787 at 284.334 kb on - strand, within GFF3787 at 284.342 kb on + strand, within GFF3787 at 284.342 kb on + strand, within GFF3787 at 284.342 kb on + strand, within GFF3787 at 284.426 kb on + strand, within GFF3787 at 284.426 kb on + strand, within GFF3787 at 284.427 kb on - strand, within GFF3787 at 284.427 kb on - strand, within GFF3787 at 285.114 kb on - strand at 285.148 kb on + strand at 285.149 kb on - strand at 285.175 kb on - strand at 285.175 kb on - strand at 285.175 kb on - strand at 285.175 kb on - strand at 285.712 kb on + strand, within GFF3788 at 285.712 kb on + strand, within GFF3788 at 285.712 kb on + strand, within GFF3788 at 285.712 kb on + strand, within GFF3788 at 285.712 kb on + strand, within GFF3788 at 285.713 kb on - strand, within GFF3788 at 285.713 kb on - strand, within GFF3788 at 285.745 kb on + strand, within GFF3788 at 285.746 kb on - strand, within GFF3788 at 285.746 kb on - strand, within GFF3788 at 285.746 kb on - strand, within GFF3788 at 285.746 kb on - strand, within GFF3788 at 285.746 kb on - strand, within GFF3788 at 285.940 kb on + strand, within GFF3788 at 286.435 kb on + strand at 286.436 kb on - strand at 286.436 kb on - strand at 286.436 kb on - strand at 286.556 kb on + strand at 286.556 kb on + strand at 286.556 kb on + strand at 286.556 kb on + strand at 286.556 kb on + strand at 286.556 kb on + strand at 286.557 kb on - strand at 286.557 kb on - strand at 286.557 kb on - strand at 286.557 kb on - strand at 286.560 kb on + strand at 286.560 kb on + strand at 286.560 kb on + strand at 286.561 kb on - strand at 286.561 kb on - strand at 286.561 kb on - strand at 286.561 kb on - strand at 286.823 kb on + strand, within GFF3789 at 286.823 kb on + strand, within GFF3789 at 286.823 kb on + strand, within GFF3789 at 286.824 kb on - strand, within GFF3789 at 286.824 kb on - strand, within GFF3789 at 286.824 kb on - strand, within GFF3789 at 286.862 kb on + strand, within GFF3789 at 286.887 kb on - strand, within GFF3789 at 286.887 kb on - strand, within GFF3789 at 286.892 kb on + strand, within GFF3789 at 286.934 kb on + strand, within GFF3789 at 286.934 kb on + strand, within GFF3789 at 286.934 kb on + strand, within GFF3789 at 286.934 kb on + strand, within GFF3789 at 286.934 kb on + strand, within GFF3789 at 286.934 kb on + strand, within GFF3789 at 286.935 kb on - strand, within GFF3789 at 286.935 kb on - strand, within GFF3789 at 286.935 kb on - strand, within GFF3789 at 286.935 kb on - strand, within GFF3789 at 286.982 kb on + strand at 286.983 kb on - strand at 287.006 kb on + strand at 287.020 kb on + strand at 287.021 kb on - strand at 287.021 kb on - strand at 287.021 kb on - strand at 287.142 kb on - strand, within GFF3790 at 287.209 kb on + strand, within GFF3790 at 287.209 kb on + strand, within GFF3790 at 287.210 kb on - strand, within GFF3790 at 287.257 kb on + strand, within GFF3790 at 287.359 kb on + strand, within GFF3790 at 287.360 kb on - strand, within GFF3790 at 287.360 kb on - strand, within GFF3790 at 287.474 kb on - strand, within GFF3790 at 287.474 kb on - strand, within GFF3790 at 287.492 kb on - strand, within GFF3790 at 287.492 kb on - strand, within GFF3790 at 287.492 kb on - strand, within GFF3790
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 284,333 + GFF3787 0.76 +0.5 284,333 + GFF3787 0.76 -1.1 284,333 + GFF3787 0.76 -0.2 284,334 - GFF3787 0.76 -0.7 284,334 - GFF3787 0.76 -0.9 284,334 - GFF3787 0.76 +0.3 284,342 + GFF3787 0.76 +2.0 284,342 + GFF3787 0.76 -0.0 284,342 + GFF3787 0.76 -1.6 284,426 + GFF3787 0.79 +0.3 284,426 + GFF3787 0.79 -0.4 284,427 - GFF3787 0.79 -1.2 284,427 - GFF3787 0.79 -0.1 285,114 - -0.7 285,148 + +0.2 285,149 - -0.6 285,175 - +0.2 285,175 - -0.6 285,175 - +0.7 285,175 - -0.8 285,712 + GFF3788 0.39 -0.2 285,712 + GFF3788 0.39 -0.1 285,712 + GFF3788 0.39 -1.1 285,712 + GFF3788 0.39 -0.2 285,712 + GFF3788 0.39 -0.3 285,713 - GFF3788 0.39 +0.7 285,713 - GFF3788 0.39 -0.2 285,745 + GFF3788 0.41 -1.2 285,746 - GFF3788 0.41 -0.3 285,746 - GFF3788 0.41 -1.0 285,746 - GFF3788 0.41 -0.6 285,746 - GFF3788 0.41 -1.1 285,746 - GFF3788 0.41 -0.0 285,940 + GFF3788 0.56 -0.7 286,435 + +0.1 286,436 - +0.3 286,436 - +0.5 286,436 - -0.3 286,556 + -0.3 286,556 + -0.1 286,556 + -0.9 286,556 + -0.1 286,556 + -1.2 286,556 + +1.1 286,557 - -0.4 286,557 - +0.7 286,557 - -1.4 286,557 - -0.6 286,560 + +1.9 286,560 + +1.4 286,560 + -0.9 286,561 - -1.1 286,561 - -0.9 286,561 - +1.7 286,561 - -0.9 286,823 + GFF3789 0.48 -0.7 286,823 + GFF3789 0.48 +0.7 286,823 + GFF3789 0.48 +1.9 286,824 - GFF3789 0.48 +0.6 286,824 - GFF3789 0.48 -0.4 286,824 - GFF3789 0.48 -0.7 286,862 + GFF3789 0.58 -1.1 286,887 - GFF3789 0.65 -0.4 286,887 - GFF3789 0.65 -1.1 286,892 + GFF3789 0.66 +1.5 286,934 + GFF3789 0.78 +0.4 286,934 + GFF3789 0.78 +0.9 286,934 + GFF3789 0.78 -0.7 286,934 + GFF3789 0.78 +0.1 286,934 + GFF3789 0.78 -0.5 286,934 + GFF3789 0.78 +0.0 286,935 - GFF3789 0.78 +0.2 286,935 - GFF3789 0.78 -1.1 286,935 - GFF3789 0.78 -0.3 286,935 - GFF3789 0.78 -1.4 286,982 + -1.1 286,983 - -1.3 287,006 + +0.3 287,020 + -0.0 287,021 - +0.4 287,021 - -0.6 287,021 - -0.3 287,142 - GFF3790 0.14 -0.3 287,209 + GFF3790 0.22 +0.5 287,209 + GFF3790 0.22 -0.9 287,210 - GFF3790 0.22 -1.1 287,257 + GFF3790 0.27 -0.1 287,359 + GFF3790 0.39 +1.7 287,360 - GFF3790 0.39 -2.1 287,360 - GFF3790 0.39 -0.6 287,474 - GFF3790 0.52 +0.3 287,474 - GFF3790 0.52 +0.3 287,492 - GFF3790 0.55 +1.1 287,492 - GFF3790 0.55 -0.4 287,492 - GFF3790 0.55 -1.9
Or see this region's nucleotide sequence