Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF359 and GFF360 overlap by 4 nucleotides GFF360 and GFF361 are separated by 131 nucleotides
GFF359 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 389,167 to 390,174
GFF359
GFF360 - Putative transporter, at 390,171 to 391,328
GFF360
GFF361 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 391,460 to 392,980
GFF361
Position (kb) 390
391
392 Strain fitness (log2 ratio) -2
-1
0
1
2 at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.191 kb on + strand at 389.191 kb on + strand at 389.191 kb on + strand at 389.192 kb on - strand at 389.192 kb on - strand at 389.192 kb on - strand at 389.239 kb on + strand at 389.239 kb on + strand at 389.239 kb on + strand at 389.240 kb on - strand at 389.240 kb on - strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.650 kb on + strand, within GFF359 at 389.681 kb on - strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.073 kb on + strand, within GFF359 at 390.073 kb on + strand, within GFF359 at 390.074 kb on - strand at 390.074 kb on - strand at 390.074 kb on - strand at 390.074 kb on - strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.335 kb on - strand, within GFF360 at 390.335 kb on - strand, within GFF360 at 390.417 kb on + strand, within GFF360 at 390.417 kb on + strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.846 kb on + strand, within GFF360 at 390.847 kb on - strand, within GFF360 at 390.847 kb on - strand, within GFF360 at 390.847 kb on - strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.182 kb on + strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.183 kb on - strand, within GFF360 at 391.345 kb on - strand at 391.431 kb on - strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.472 kb on + strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.473 kb on - strand at 391.491 kb on + strand at 391.491 kb on + strand at 391.492 kb on - strand at 391.492 kb on - strand at 391.492 kb on - strand at 391.664 kb on + strand, within GFF361 at 391.664 kb on + strand, within GFF361 at 391.664 kb on + strand, within GFF361 at 391.664 kb on + strand, within GFF361 at 391.665 kb on - strand, within GFF361 at 391.665 kb on - strand, within GFF361 at 391.993 kb on + strand, within GFF361 at 391.993 kb on + strand, within GFF361 at 392.052 kb on - strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.207 kb on + strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.208 kb on - strand, within GFF361 at 392.216 kb on + strand, within GFF361 at 392.216 kb on + strand, within GFF361 at 392.216 kb on + strand, within GFF361 at 392.217 kb on - strand, within GFF361 at 392.217 kb on - strand, within GFF361 at 392.306 kb on + strand, within GFF361 at 392.306 kb on + strand, within GFF361 at 392.306 kb on + strand, within GFF361 at 392.307 kb on - strand, within GFF361 at 392.307 kb on - strand, within GFF361 at 392.312 kb on + strand, within GFF361 at 392.313 kb on - strand, within GFF361
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 389,172 + -0.5 389,172 + +0.1 389,172 + -1.7 389,172 + +0.0 389,172 + -0.3 389,172 + -0.8 389,173 - +1.3 389,173 - +0.2 389,173 - -1.8 389,173 - -0.3 389,173 - +0.5 389,173 - -0.9 389,173 - -0.9 389,173 - -0.4 389,173 - -0.8 389,191 + +0.2 389,191 + +0.6 389,191 + +0.2 389,192 - +0.5 389,192 - -1.3 389,192 - -0.3 389,239 + -0.6 389,239 + -0.8 389,239 + -0.5 389,240 - -0.4 389,240 - -0.3 389,254 + -0.3 389,254 + -0.0 389,254 + -0.6 389,254 + +1.9 389,254 + +0.7 389,254 + +0.3 389,254 + +0.6 389,254 + -0.4 389,255 - +1.1 389,255 - +1.0 389,255 - +0.7 389,255 - -0.3 389,255 - -0.1 389,255 - -0.2 389,255 - +0.8 389,255 - -0.7 389,256 + -0.1 389,256 + +1.1 389,256 + -0.1 389,256 + +0.1 389,256 + -0.6 389,256 + +0.4 389,256 + -1.3 389,256 + -0.7 389,257 - +0.9 389,257 - -0.2 389,257 - +0.5 389,257 - +0.6 389,257 - +0.9 389,257 - -0.4 389,257 - -0.7 389,257 - +0.7 389,257 - -0.6 389,257 - -0.4 389,533 + GFF359 0.36 -0.0 389,533 + GFF359 0.36 -0.7 389,533 + GFF359 0.36 -0.5 389,533 + GFF359 0.36 -0.5 389,533 + GFF359 0.36 +0.4 389,533 + GFF359 0.36 -0.6 389,533 + GFF359 0.36 -0.5 389,533 + GFF359 0.36 -0.8 389,533 + GFF359 0.36 -0.5 389,533 + GFF359 0.36 -0.1 389,533 + GFF359 0.36 -0.7 389,533 + GFF359 0.36 +2.1 389,534 - GFF359 0.36 -1.5 389,534 - GFF359 0.36 -0.8 389,534 - GFF359 0.36 -1.5 389,534 - GFF359 0.36 -2.2 389,534 - GFF359 0.36 -0.9 389,534 - GFF359 0.36 -0.2 389,534 - GFF359 0.36 -0.8 389,534 - GFF359 0.36 -0.9 389,534 - GFF359 0.36 -0.4 389,534 - GFF359 0.36 -0.5 389,534 - GFF359 0.36 +0.3 389,650 + GFF359 0.48 -1.7 389,681 - GFF359 0.51 +0.4 390,055 + GFF359 0.88 -0.7 390,055 + GFF359 0.88 -0.2 390,055 + GFF359 0.88 -0.1 390,056 - GFF359 0.88 -0.6 390,056 - GFF359 0.88 -2.0 390,056 - GFF359 0.88 +0.8 390,056 - GFF359 0.88 -0.0 390,073 + GFF359 0.90 -1.0 390,073 + GFF359 0.90 +0.1 390,074 - +0.3 390,074 - -0.1 390,074 - -0.4 390,074 - -0.6 390,082 + -0.8 390,082 + +0.7 390,082 + -1.2 390,082 + -0.3 390,083 - -1.0 390,083 - -1.7 390,083 - -0.5 390,083 - -0.5 390,083 - -1.0 390,083 - -0.8 390,083 - -0.2 390,115 + -0.5 390,115 + +0.1 390,115 + -1.5 390,115 + -0.2 390,115 + -0.1 390,115 + -0.4 390,115 + -1.0 390,115 + -1.3 390,115 + +0.5 390,115 + +0.9 390,115 + +0.2 390,115 + -1.1 390,116 - -0.8 390,116 - -0.1 390,116 - -0.5 390,116 - -0.3 390,116 - -0.6 390,116 - +0.2 390,116 - -0.2 390,116 - -0.0 390,116 - -0.7 390,116 - -1.1 390,116 - +0.1 390,116 - -1.8 390,116 - -1.2 390,335 - GFF360 0.14 +1.3 390,335 - GFF360 0.14 +1.1 390,417 + GFF360 0.21 -0.6 390,417 + GFF360 0.21 +1.0 390,418 - GFF360 0.21 +0.3 390,418 - GFF360 0.21 -0.6 390,418 - GFF360 0.21 +0.7 390,646 + GFF360 0.41 -0.5 390,646 + GFF360 0.41 -0.2 390,646 + GFF360 0.41 +0.2 390,646 + GFF360 0.41 -1.0 390,646 + GFF360 0.41 +0.5 390,646 + GFF360 0.41 -0.7 390,647 - GFF360 0.41 +0.2 390,647 - GFF360 0.41 +0.3 390,647 - GFF360 0.41 -0.5 390,647 - GFF360 0.41 -0.1 390,647 - GFF360 0.41 +1.6 390,647 - GFF360 0.41 -0.3 390,647 - GFF360 0.41 +2.0 390,647 - GFF360 0.41 +0.2 390,647 - GFF360 0.41 +0.5 390,647 - GFF360 0.41 +0.7 390,846 + GFF360 0.58 -1.5 390,847 - GFF360 0.58 +0.2 390,847 - GFF360 0.58 -0.3 390,847 - GFF360 0.58 +0.4 391,182 + GFF360 0.87 -0.0 391,182 + GFF360 0.87 +0.4 391,182 + GFF360 0.87 -0.4 391,182 + GFF360 0.87 -1.8 391,182 + GFF360 0.87 -0.7 391,182 + GFF360 0.87 +1.7 391,182 + GFF360 0.87 +0.8 391,182 + GFF360 0.87 -0.1 391,182 + GFF360 0.87 -0.4 391,183 - GFF360 0.87 -1.5 391,183 - GFF360 0.87 -1.0 391,183 - GFF360 0.87 -0.7 391,183 - GFF360 0.87 -0.0 391,183 - GFF360 0.87 -0.2 391,183 - GFF360 0.87 -0.5 391,183 - GFF360 0.87 -1.5 391,183 - GFF360 0.87 +0.7 391,183 - GFF360 0.87 +2.3 391,345 - -2.2 391,431 - -0.6 391,472 + +1.1 391,472 + -0.5 391,472 + -1.6 391,472 + +0.6 391,472 + -0.5 391,472 + +0.3 391,472 + -0.6 391,472 + -0.7 391,473 - -0.9 391,473 - -0.1 391,473 - -1.3 391,473 - -0.9 391,473 - -1.3 391,473 - -0.7 391,491 + +0.4 391,491 + -0.1 391,492 - -0.5 391,492 - +0.1 391,492 - +0.2 391,664 + GFF361 0.13 +0.7 391,664 + GFF361 0.13 +1.3 391,664 + GFF361 0.13 +0.8 391,664 + GFF361 0.13 -1.1 391,665 - GFF361 0.13 +0.7 391,665 - GFF361 0.13 -0.5 391,993 + GFF361 0.35 -0.6 391,993 + GFF361 0.35 -2.0 392,052 - GFF361 0.39 +0.1 392,207 + GFF361 0.49 -1.2 392,207 + GFF361 0.49 +1.1 392,207 + GFF361 0.49 -0.5 392,207 + GFF361 0.49 -0.9 392,207 + GFF361 0.49 +0.3 392,207 + GFF361 0.49 -1.5 392,207 + GFF361 0.49 +0.2 392,208 - GFF361 0.49 -0.7 392,208 - GFF361 0.49 +0.9 392,208 - GFF361 0.49 -0.3 392,208 - GFF361 0.49 -1.5 392,208 - GFF361 0.49 -0.3 392,208 - GFF361 0.49 -1.0 392,208 - GFF361 0.49 -0.8 392,208 - GFF361 0.49 -1.1 392,208 - GFF361 0.49 -0.0 392,208 - GFF361 0.49 -0.0 392,208 - GFF361 0.49 +0.5 392,216 + GFF361 0.50 -1.0 392,216 + GFF361 0.50 -1.7 392,216 + GFF361 0.50 +1.3 392,217 - GFF361 0.50 +0.5 392,217 - GFF361 0.50 +0.7 392,306 + GFF361 0.56 -1.0 392,306 + GFF361 0.56 -0.2 392,306 + GFF361 0.56 +0.7 392,307 - GFF361 0.56 +0.3 392,307 - GFF361 0.56 -0.8 392,312 + GFF361 0.56 +0.3 392,313 - GFF361 0.56 -0.2
Or see this region's nucleotide sequence