Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3476 and GFF3477 are separated by 95 nucleotides GFF3477 and GFF3478 overlap by 4 nucleotides
GFF3476 - Cardiolipin synthase (EC 2.7.8.-) phosphatidylethanolamine-utilizing, bacterial type ClsC, at 297,163 to 298,746
GFF3476
GFF3477 - Phosphoprotein phosphatase, at 298,842 to 300,410
GFF3477
GFF3478 - Cytochrome c peroxidase (EC 1.11.1.5), at 300,407 to 301,759
GFF3478
Position (kb)
298
299
300
301 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 298.045 kb on + strand, within GFF3476 at 298.046 kb on - strand, within GFF3476 at 298.112 kb on - strand, within GFF3476 at 298.112 kb on - strand, within GFF3476 at 298.186 kb on + strand, within GFF3476 at 298.186 kb on + strand, within GFF3476 at 298.187 kb on - strand, within GFF3476 at 298.307 kb on - strand, within GFF3476 at 298.351 kb on + strand, within GFF3476 at 298.549 kb on + strand, within GFF3476 at 298.550 kb on - strand, within GFF3476 at 298.550 kb on - strand, within GFF3476 at 298.636 kb on + strand at 298.636 kb on + strand at 298.637 kb on - strand at 298.637 kb on - strand at 298.637 kb on - strand at 298.744 kb on + strand at 298.770 kb on + strand at 298.770 kb on + strand at 298.770 kb on + strand at 298.771 kb on - strand at 298.771 kb on - strand at 298.773 kb on + strand at 298.773 kb on + strand at 298.773 kb on + strand at 298.774 kb on - strand at 298.774 kb on - strand at 298.795 kb on + strand at 298.819 kb on + strand at 299.118 kb on + strand, within GFF3477 at 299.118 kb on + strand, within GFF3477 at 299.119 kb on - strand, within GFF3477 at 299.119 kb on - strand, within GFF3477 at 299.524 kb on - strand, within GFF3477 at 299.529 kb on + strand, within GFF3477 at 299.731 kb on - strand, within GFF3477 at 299.731 kb on - strand, within GFF3477 at 299.731 kb on - strand, within GFF3477 at 299.781 kb on + strand, within GFF3477 at 299.781 kb on + strand, within GFF3477 at 299.782 kb on - strand, within GFF3477 at 299.782 kb on - strand, within GFF3477 at 299.782 kb on - strand, within GFF3477 at 299.782 kb on - strand, within GFF3477 at 300.060 kb on + strand, within GFF3477 at 300.060 kb on + strand, within GFF3477 at 300.060 kb on + strand, within GFF3477 at 300.061 kb on - strand, within GFF3477 at 300.061 kb on - strand, within GFF3477 at 300.064 kb on - strand, within GFF3477 at 300.321 kb on + strand at 300.321 kb on + strand at 300.321 kb on + strand at 300.321 kb on + strand at 300.322 kb on - strand at 300.322 kb on - strand at 300.322 kb on - strand at 300.833 kb on + strand, within GFF3478 at 300.833 kb on + strand, within GFF3478 at 300.833 kb on + strand, within GFF3478 at 300.833 kb on + strand, within GFF3478 at 300.833 kb on + strand, within GFF3478 at 300.834 kb on - strand, within GFF3478 at 300.834 kb on - strand, within GFF3478 at 300.842 kb on + strand, within GFF3478 at 300.843 kb on - strand, within GFF3478 at 300.863 kb on + strand, within GFF3478 at 300.864 kb on - strand, within GFF3478 at 300.864 kb on - strand, within GFF3478 at 300.864 kb on - strand, within GFF3478 at 301.115 kb on + strand, within GFF3478 at 301.115 kb on + strand, within GFF3478 at 301.115 kb on + strand, within GFF3478 at 301.116 kb on - strand, within GFF3478 at 301.116 kb on - strand, within GFF3478 at 301.116 kb on - strand, within GFF3478 at 301.163 kb on + strand, within GFF3478 at 301.164 kb on - strand, within GFF3478 at 301.164 kb on - strand, within GFF3478 at 301.311 kb on - strand, within GFF3478
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 298,045 + GFF3476 0.56 +0.5 298,046 - GFF3476 0.56 -0.6 298,112 - GFF3476 0.60 -0.7 298,112 - GFF3476 0.60 -2.2 298,186 + GFF3476 0.65 +0.4 298,186 + GFF3476 0.65 +0.3 298,187 - GFF3476 0.65 -1.0 298,307 - GFF3476 0.72 +1.0 298,351 + GFF3476 0.75 -1.7 298,549 + GFF3476 0.88 -0.9 298,550 - GFF3476 0.88 -0.4 298,550 - GFF3476 0.88 +0.5 298,636 + -0.7 298,636 + -0.4 298,637 - -0.0 298,637 - +0.4 298,637 - -0.3 298,744 + +0.1 298,770 + +0.4 298,770 + +0.2 298,770 + +0.4 298,771 - +0.0 298,771 - +0.2 298,773 + -0.3 298,773 + -0.5 298,773 + +0.1 298,774 - +0.2 298,774 - -0.9 298,795 + +1.5 298,819 + -0.8 299,118 + GFF3477 0.18 -0.9 299,118 + GFF3477 0.18 +0.2 299,119 - GFF3477 0.18 -0.1 299,119 - GFF3477 0.18 -0.7 299,524 - GFF3477 0.43 -0.5 299,529 + GFF3477 0.44 -1.7 299,731 - GFF3477 0.57 +1.4 299,731 - GFF3477 0.57 +1.3 299,731 - GFF3477 0.57 +0.4 299,781 + GFF3477 0.60 -0.1 299,781 + GFF3477 0.60 -0.0 299,782 - GFF3477 0.60 -0.4 299,782 - GFF3477 0.60 -0.4 299,782 - GFF3477 0.60 -0.2 299,782 - GFF3477 0.60 -1.0 300,060 + GFF3477 0.78 +0.2 300,060 + GFF3477 0.78 -1.3 300,060 + GFF3477 0.78 +0.4 300,061 - GFF3477 0.78 -2.1 300,061 - GFF3477 0.78 -1.1 300,064 - GFF3477 0.78 -0.8 300,321 + -0.3 300,321 + -0.9 300,321 + +0.1 300,321 + -0.8 300,322 - -0.8 300,322 - +0.1 300,322 - +0.9 300,833 + GFF3478 0.31 -0.5 300,833 + GFF3478 0.31 -0.0 300,833 + GFF3478 0.31 -1.3 300,833 + GFF3478 0.31 -0.1 300,833 + GFF3478 0.31 +0.2 300,834 - GFF3478 0.32 -3.2 300,834 - GFF3478 0.32 -0.3 300,842 + GFF3478 0.32 -0.3 300,843 - GFF3478 0.32 -0.5 300,863 + GFF3478 0.34 +0.7 300,864 - GFF3478 0.34 +1.3 300,864 - GFF3478 0.34 -0.5 300,864 - GFF3478 0.34 -0.6 301,115 + GFF3478 0.52 -1.3 301,115 + GFF3478 0.52 -0.3 301,115 + GFF3478 0.52 +0.1 301,116 - GFF3478 0.52 -0.1 301,116 - GFF3478 0.52 +0.2 301,116 - GFF3478 0.52 -0.1 301,163 + GFF3478 0.56 -0.4 301,164 - GFF3478 0.56 -0.8 301,164 - GFF3478 0.56 +0.2 301,311 - GFF3478 0.67 -0.2
Or see this region's nucleotide sequence