Strain Fitness in Variovorax sp. SCN45 around GFF337

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF335 and GFF336 are separated by 14 nucleotidesGFF336 and GFF337 overlap by 48 nucleotidesGFF337 and GFF338 are separated by 12 nucleotidesGFF338 and GFF339 are separated by 23 nucleotides GFF335 - Cytosine deaminase (EC 3.5.4.1), at 368,475 to 369,755 GFF335 GFF336 - no description, at 369,770 to 370,615 GFF336 GFF337 - hypothetical protein, at 370,568 to 371,173 GFF337 GFF338 - Chloramphenicol acetyltransferase (EC 2.3.1.28), at 371,186 to 371,932 GFF338 GFF339 - Exodeoxyribonuclease I (EC 3.1.11.1), at 371,956 to 373,395 GFF339 Position (kb) 370 371 372Strain fitness (log2 ratio) -2 -1 0 1 2at 369.870 kb on - strand, within GFF336at 369.870 kb on - strand, within GFF336at 369.902 kb on + strand, within GFF336at 369.902 kb on + strand, within GFF336at 369.902 kb on + strand, within GFF336at 369.902 kb on + strand, within GFF336at 369.902 kb on + strand, within GFF336at 369.903 kb on - strand, within GFF336at 369.903 kb on - strand, within GFF336at 369.903 kb on - strand, within GFF336at 369.903 kb on - strand, within GFF336at 369.965 kb on + strand, within GFF336at 370.053 kb on - strand, within GFF336at 370.325 kb on + strand, within GFF336at 370.325 kb on + strand, within GFF336at 370.325 kb on + strand, within GFF336at 370.325 kb on + strand, within GFF336at 370.325 kb on + strand, within GFF336at 370.325 kb on + strand, within GFF336at 370.326 kb on - strand, within GFF336at 370.326 kb on - strand, within GFF336at 370.326 kb on - strand, within GFF336at 370.326 kb on - strand, within GFF336at 370.326 kb on - strand, within GFF336at 370.562 kb on + strandat 370.562 kb on + strandat 370.562 kb on + strandat 370.562 kb on + strandat 370.562 kb on + strandat 370.562 kb on + strandat 370.562 kb on + strandat 370.562 kb on + strandat 370.563 kb on - strandat 370.563 kb on - strandat 370.563 kb on - strandat 370.563 kb on - strandat 370.563 kb on - strandat 370.563 kb on - strandat 370.722 kb on + strand, within GFF337at 370.722 kb on + strand, within GFF337at 370.723 kb on - strand, within GFF337at 370.723 kb on - strand, within GFF337at 370.723 kb on - strand, within GFF337at 370.959 kb on + strand, within GFF337at 370.959 kb on + strand, within GFF337at 370.959 kb on + strand, within GFF337at 370.960 kb on - strand, within GFF337at 370.960 kb on - strand, within GFF337at 370.960 kb on - strand, within GFF337at 371.076 kb on + strand, within GFF337at 371.077 kb on - strand, within GFF337at 371.077 kb on - strand, within GFF337at 371.101 kb on - strand, within GFF337at 371.450 kb on + strand, within GFF338at 371.451 kb on - strand, within GFF338at 371.451 kb on - strand, within GFF338at 371.451 kb on - strand, within GFF338at 371.451 kb on - strand, within GFF338at 371.567 kb on + strand, within GFF338at 371.567 kb on + strand, within GFF338at 371.567 kb on + strand, within GFF338at 371.567 kb on + strand, within GFF338at 371.567 kb on + strand, within GFF338at 371.567 kb on + strand, within GFF338at 371.568 kb on - strand, within GFF338at 371.568 kb on - strand, within GFF338at 371.568 kb on - strand, within GFF338at 371.568 kb on - strand, within GFF338at 371.568 kb on - strand, within GFF338at 371.568 kb on - strand, within GFF338at 371.568 kb on - strand, within GFF338at 371.717 kb on + strand, within GFF338at 371.727 kb on + strand, within GFF338at 371.772 kb on + strand, within GFF338at 371.772 kb on + strand, within GFF338at 371.772 kb on + strand, within GFF338at 371.772 kb on + strand, within GFF338at 371.772 kb on + strand, within GFF338at 371.772 kb on + strand, within GFF338at 371.772 kb on + strand, within GFF338at 371.773 kb on + strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.773 kb on - strand, within GFF338at 371.778 kb on + strand, within GFF338at 371.778 kb on + strand, within GFF338at 371.779 kb on - strand, within GFF338at 371.799 kb on + strand, within GFF338at 371.799 kb on + strand, within GFF338at 371.800 kb on - strand, within GFF338at 371.991 kb on - strandat 371.991 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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369,870 - GFF336 0.12 -0.7
369,870 - GFF336 0.12 -0.2
369,902 + GFF336 0.16 -0.8
369,902 + GFF336 0.16 +0.1
369,902 + GFF336 0.16 +0.5
369,902 + GFF336 0.16 -0.7
369,902 + GFF336 0.16 +0.7
369,903 - GFF336 0.16 +0.6
369,903 - GFF336 0.16 -0.8
369,903 - GFF336 0.16 -0.1
369,903 - GFF336 0.16 -0.3
369,965 + GFF336 0.23 +0.2
370,053 - GFF336 0.33 +0.1
370,325 + GFF336 0.66 +1.0
370,325 + GFF336 0.66 -0.5
370,325 + GFF336 0.66 +0.5
370,325 + GFF336 0.66 +0.6
370,325 + GFF336 0.66 -0.2
370,325 + GFF336 0.66 -1.0
370,326 - GFF336 0.66 +0.3
370,326 - GFF336 0.66 +1.7
370,326 - GFF336 0.66 -0.7
370,326 - GFF336 0.66 -0.0
370,326 - GFF336 0.66 -0.5
370,562 + -0.1
370,562 + +0.7
370,562 + -0.6
370,562 + -0.6
370,562 + -1.0
370,562 + -0.1
370,562 + +0.1
370,562 + +0.7
370,563 - +0.7
370,563 - -2.5
370,563 - +0.1
370,563 - +0.7
370,563 - -1.2
370,563 - -0.2
370,722 + GFF337 0.25 +0.9
370,722 + GFF337 0.25 +0.1
370,723 - GFF337 0.26 +0.1
370,723 - GFF337 0.26 -0.0
370,723 - GFF337 0.26 +0.3
370,959 + GFF337 0.65 +0.5
370,959 + GFF337 0.65 +1.3
370,959 + GFF337 0.65 -0.7
370,960 - GFF337 0.65 -0.4
370,960 - GFF337 0.65 +0.5
370,960 - GFF337 0.65 -0.2
371,076 + GFF337 0.84 -1.9
371,077 - GFF337 0.84 +0.1
371,077 - GFF337 0.84 +0.3
371,101 - GFF337 0.88 -0.8
371,450 + GFF338 0.35 -0.1
371,451 - GFF338 0.35 +0.1
371,451 - GFF338 0.35 +0.6
371,451 - GFF338 0.35 -0.0
371,451 - GFF338 0.35 +0.8
371,567 + GFF338 0.51 -0.4
371,567 + GFF338 0.51 -0.3
371,567 + GFF338 0.51 -0.4
371,567 + GFF338 0.51 -0.4
371,567 + GFF338 0.51 +0.3
371,567 + GFF338 0.51 -0.9
371,568 - GFF338 0.51 +1.9
371,568 - GFF338 0.51 +0.0
371,568 - GFF338 0.51 -0.9
371,568 - GFF338 0.51 -1.2
371,568 - GFF338 0.51 -0.2
371,568 - GFF338 0.51 -0.1
371,568 - GFF338 0.51 -0.6
371,717 + GFF338 0.71 -1.5
371,727 + GFF338 0.72 -0.5
371,772 + GFF338 0.78 -0.2
371,772 + GFF338 0.78 -0.3
371,772 + GFF338 0.78 -1.2
371,772 + GFF338 0.78 -0.0
371,772 + GFF338 0.78 -0.9
371,772 + GFF338 0.78 -1.0
371,772 + GFF338 0.78 -0.5
371,773 + GFF338 0.79 +1.3
371,773 - GFF338 0.79 -0.5
371,773 - GFF338 0.79 -1.4
371,773 - GFF338 0.79 +0.8
371,773 - GFF338 0.79 -0.6
371,773 - GFF338 0.79 -0.1
371,773 - GFF338 0.79 -0.1
371,773 - GFF338 0.79 -0.3
371,773 - GFF338 0.79 +0.4
371,773 - GFF338 0.79 -1.2
371,778 + GFF338 0.79 -1.5
371,778 + GFF338 0.79 +0.3
371,779 - GFF338 0.79 +1.3
371,799 + GFF338 0.82 +0.8
371,799 + GFF338 0.82 -0.1
371,800 - GFF338 0.82 -0.3
371,991 - -1.6
371,991 - -0.7

Or see this region's nucleotide sequence