Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3299 and GFF3300 are separated by 46 nucleotides GFF3300 and GFF3301 overlap by 4 nucleotides GFF3301 and GFF3302 are separated by 129 nucleotides GFF3302 and GFF3303 overlap by 4 nucleotides
GFF3299 - Pirin, at 123,322 to 124,218
GFF3299
GFF3300 - hypothetical protein, at 124,265 to 124,822
GFF3300
GFF3301 - Lignostilbene-alpha,beta-dioxygenase and related enzymes, at 124,819 to 126,246
GFF3301
GFF3302 - no description, at 126,376 to 127,017
GFF3302
GFF3303 - Acetyltransferase, GNAT family, at 127,014 to 127,778
GFF3303
Position (kb)
124
125
126
127 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.968 kb on + strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 123.969 kb on - strand, within GFF3299 at 124.020 kb on - strand, within GFF3299 at 124.020 kb on - strand, within GFF3299 at 124.020 kb on - strand, within GFF3299 at 124.061 kb on + strand, within GFF3299 at 124.061 kb on + strand, within GFF3299 at 124.061 kb on + strand, within GFF3299 at 124.061 kb on + strand, within GFF3299 at 124.062 kb on - strand, within GFF3299 at 124.062 kb on - strand, within GFF3299 at 124.236 kb on - strand at 124.305 kb on + strand at 124.306 kb on - strand at 124.306 kb on - strand at 124.386 kb on + strand, within GFF3300 at 124.386 kb on + strand, within GFF3300 at 124.386 kb on + strand, within GFF3300 at 124.386 kb on + strand, within GFF3300 at 124.386 kb on + strand, within GFF3300 at 124.386 kb on + strand, within GFF3300 at 124.386 kb on + strand, within GFF3300 at 124.387 kb on - strand, within GFF3300 at 124.387 kb on - strand, within GFF3300 at 124.387 kb on - strand, within GFF3300 at 124.387 kb on - strand, within GFF3300 at 124.387 kb on - strand, within GFF3300 at 124.387 kb on - strand, within GFF3300 at 124.387 kb on - strand, within GFF3300 at 124.387 kb on - strand, within GFF3300 at 124.512 kb on + strand, within GFF3300 at 124.513 kb on - strand, within GFF3300 at 124.629 kb on + strand, within GFF3300 at 124.629 kb on + strand, within GFF3300 at 124.629 kb on + strand, within GFF3300 at 124.629 kb on + strand, within GFF3300 at 124.630 kb on - strand, within GFF3300 at 124.630 kb on - strand, within GFF3300 at 124.630 kb on - strand, within GFF3300 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.069 kb on + strand, within GFF3301 at 125.070 kb on - strand, within GFF3301 at 125.070 kb on - strand, within GFF3301 at 125.070 kb on - strand, within GFF3301 at 125.070 kb on - strand, within GFF3301 at 125.070 kb on - strand, within GFF3301 at 125.070 kb on - strand, within GFF3301 at 125.117 kb on + strand, within GFF3301 at 125.117 kb on + strand, within GFF3301 at 125.118 kb on - strand, within GFF3301 at 125.118 kb on - strand, within GFF3301 at 125.118 kb on - strand, within GFF3301 at 125.118 kb on - strand, within GFF3301 at 125.162 kb on + strand, within GFF3301 at 125.162 kb on + strand, within GFF3301 at 125.162 kb on + strand, within GFF3301 at 125.162 kb on + strand, within GFF3301 at 125.162 kb on + strand, within GFF3301 at 125.162 kb on + strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.163 kb on - strand, within GFF3301 at 125.177 kb on + strand, within GFF3301 at 125.178 kb on - strand, within GFF3301 at 125.356 kb on - strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.423 kb on + strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.424 kb on - strand, within GFF3301 at 125.498 kb on + strand, within GFF3301 at 125.499 kb on - strand, within GFF3301 at 125.499 kb on - strand, within GFF3301 at 125.621 kb on + strand, within GFF3301 at 125.622 kb on - strand, within GFF3301 at 125.622 kb on - strand, within GFF3301 at 126.023 kb on + strand, within GFF3301 at 126.207 kb on + strand at 126.207 kb on + strand at 126.207 kb on + strand at 126.286 kb on - strand at 126.355 kb on - strand at 126.406 kb on + strand at 126.407 kb on - strand at 126.407 kb on - strand at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.670 kb on + strand, within GFF3302 at 126.671 kb on - strand, within GFF3302 at 126.671 kb on - strand, within GFF3302 at 126.671 kb on - strand, within GFF3302 at 126.671 kb on - strand, within GFF3302 at 126.671 kb on - strand, within GFF3302 at 126.671 kb on - strand, within GFF3302 at 126.729 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.880 kb on + strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 126.881 kb on - strand, within GFF3302 at 127.015 kb on + strand at 127.016 kb on - strand at 127.037 kb on - strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.069 kb on + strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.070 kb on - strand at 127.070 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 123,968 + GFF3299 0.72 -0.6 123,968 + GFF3299 0.72 -0.1 123,968 + GFF3299 0.72 -0.4 123,968 + GFF3299 0.72 -1.5 123,968 + GFF3299 0.72 -0.6 123,968 + GFF3299 0.72 -2.4 123,968 + GFF3299 0.72 +0.2 123,968 + GFF3299 0.72 -0.8 123,968 + GFF3299 0.72 +1.6 123,968 + GFF3299 0.72 -0.4 123,968 + GFF3299 0.72 -1.2 123,968 + GFF3299 0.72 -0.4 123,968 + GFF3299 0.72 -1.1 123,968 + GFF3299 0.72 -1.2 123,968 + GFF3299 0.72 -0.1 123,968 + GFF3299 0.72 -0.5 123,969 - GFF3299 0.72 -0.0 123,969 - GFF3299 0.72 -1.8 123,969 - GFF3299 0.72 -0.5 123,969 - GFF3299 0.72 -0.7 123,969 - GFF3299 0.72 +1.0 123,969 - GFF3299 0.72 -0.6 123,969 - GFF3299 0.72 +0.3 123,969 - GFF3299 0.72 -0.9 123,969 - GFF3299 0.72 -0.6 123,969 - GFF3299 0.72 -1.9 123,969 - GFF3299 0.72 +0.2 123,969 - GFF3299 0.72 -1.0 123,969 - GFF3299 0.72 -0.0 123,969 - GFF3299 0.72 -0.1 123,969 - GFF3299 0.72 -0.1 124,020 - GFF3299 0.78 -0.3 124,020 - GFF3299 0.78 -0.3 124,020 - GFF3299 0.78 -0.5 124,061 + GFF3299 0.82 -0.8 124,061 + GFF3299 0.82 +0.3 124,061 + GFF3299 0.82 +0.3 124,061 + GFF3299 0.82 -0.8 124,062 - GFF3299 0.82 +0.1 124,062 - GFF3299 0.82 -0.5 124,236 - -0.4 124,305 + -0.1 124,306 - -0.3 124,306 - -0.4 124,386 + GFF3300 0.22 +0.3 124,386 + GFF3300 0.22 -0.8 124,386 + GFF3300 0.22 -0.6 124,386 + GFF3300 0.22 -0.2 124,386 + GFF3300 0.22 +0.5 124,386 + GFF3300 0.22 +0.3 124,386 + GFF3300 0.22 +0.1 124,387 - GFF3300 0.22 +0.5 124,387 - GFF3300 0.22 +0.5 124,387 - GFF3300 0.22 -1.9 124,387 - GFF3300 0.22 +0.5 124,387 - GFF3300 0.22 -1.4 124,387 - GFF3300 0.22 -2.8 124,387 - GFF3300 0.22 +0.6 124,387 - GFF3300 0.22 -1.5 124,512 + GFF3300 0.44 -0.5 124,513 - GFF3300 0.44 -0.6 124,629 + GFF3300 0.65 -0.1 124,629 + GFF3300 0.65 +0.1 124,629 + GFF3300 0.65 -0.5 124,629 + GFF3300 0.65 -0.4 124,630 - GFF3300 0.65 -1.4 124,630 - GFF3300 0.65 -0.4 124,630 - GFF3300 0.65 -1.7 125,069 + GFF3301 0.18 +0.0 125,069 + GFF3301 0.18 +0.2 125,069 + GFF3301 0.18 -0.5 125,069 + GFF3301 0.18 -0.0 125,069 + GFF3301 0.18 -0.5 125,069 + GFF3301 0.18 +1.4 125,069 + GFF3301 0.18 -0.8 125,069 + GFF3301 0.18 +0.7 125,069 + GFF3301 0.18 -0.4 125,069 + GFF3301 0.18 +1.6 125,069 + GFF3301 0.18 -0.4 125,069 + GFF3301 0.18 -0.8 125,070 - GFF3301 0.18 +1.1 125,070 - GFF3301 0.18 -1.0 125,070 - GFF3301 0.18 +0.8 125,070 - GFF3301 0.18 +1.0 125,070 - GFF3301 0.18 -0.3 125,070 - GFF3301 0.18 -0.4 125,117 + GFF3301 0.21 +0.4 125,117 + GFF3301 0.21 -0.0 125,118 - GFF3301 0.21 +0.2 125,118 - GFF3301 0.21 +0.1 125,118 - GFF3301 0.21 +0.6 125,118 - GFF3301 0.21 -0.1 125,162 + GFF3301 0.24 -0.4 125,162 + GFF3301 0.24 +1.4 125,162 + GFF3301 0.24 +1.0 125,162 + GFF3301 0.24 -0.3 125,162 + GFF3301 0.24 -0.7 125,162 + GFF3301 0.24 +0.4 125,163 - GFF3301 0.24 -0.4 125,163 - GFF3301 0.24 +0.6 125,163 - GFF3301 0.24 -0.0 125,163 - GFF3301 0.24 -0.1 125,163 - GFF3301 0.24 +0.7 125,163 - GFF3301 0.24 -0.6 125,163 - GFF3301 0.24 -1.4 125,163 - GFF3301 0.24 +0.2 125,163 - GFF3301 0.24 +0.4 125,163 - GFF3301 0.24 +0.2 125,177 + GFF3301 0.25 -0.0 125,178 - GFF3301 0.25 -0.7 125,356 - GFF3301 0.38 -0.2 125,423 + GFF3301 0.42 -2.1 125,423 + GFF3301 0.42 -0.4 125,423 + GFF3301 0.42 +1.6 125,423 + GFF3301 0.42 -1.5 125,423 + GFF3301 0.42 -0.2 125,423 + GFF3301 0.42 -0.8 125,423 + GFF3301 0.42 -0.9 125,423 + GFF3301 0.42 +0.5 125,423 + GFF3301 0.42 +0.1 125,423 + GFF3301 0.42 +0.6 125,423 + GFF3301 0.42 +0.3 125,423 + GFF3301 0.42 +0.8 125,423 + GFF3301 0.42 -0.5 125,424 - GFF3301 0.42 -0.6 125,424 - GFF3301 0.42 -1.7 125,424 - GFF3301 0.42 -0.0 125,424 - GFF3301 0.42 -1.0 125,424 - GFF3301 0.42 -0.3 125,424 - GFF3301 0.42 +0.0 125,424 - GFF3301 0.42 +0.2 125,424 - GFF3301 0.42 -0.8 125,424 - GFF3301 0.42 +0.6 125,424 - GFF3301 0.42 -0.5 125,424 - GFF3301 0.42 -1.1 125,424 - GFF3301 0.42 -1.0 125,424 - GFF3301 0.42 -0.0 125,424 - GFF3301 0.42 +0.2 125,498 + GFF3301 0.48 -0.1 125,499 - GFF3301 0.48 +0.8 125,499 - GFF3301 0.48 +0.2 125,621 + GFF3301 0.56 -0.5 125,622 - GFF3301 0.56 +1.4 125,622 - GFF3301 0.56 +0.3 126,023 + GFF3301 0.84 -0.8 126,207 + +0.2 126,207 + -1.8 126,207 + -0.1 126,286 - +0.2 126,355 - +0.1 126,406 + +0.1 126,407 - -0.1 126,407 - -1.1 126,670 + GFF3302 0.46 +2.4 126,670 + GFF3302 0.46 -0.8 126,670 + GFF3302 0.46 +2.0 126,670 + GFF3302 0.46 -0.4 126,670 + GFF3302 0.46 +1.2 126,670 + GFF3302 0.46 -0.7 126,670 + GFF3302 0.46 +0.4 126,670 + GFF3302 0.46 -0.8 126,670 + GFF3302 0.46 -0.9 126,670 + GFF3302 0.46 -1.1 126,670 + GFF3302 0.46 -0.6 126,671 - GFF3302 0.46 -1.7 126,671 - GFF3302 0.46 -0.3 126,671 - GFF3302 0.46 +0.6 126,671 - GFF3302 0.46 +0.7 126,671 - GFF3302 0.46 -0.2 126,671 - GFF3302 0.46 -0.5 126,729 + GFF3302 0.55 -0.5 126,880 + GFF3302 0.79 +0.4 126,880 + GFF3302 0.79 -0.6 126,880 + GFF3302 0.79 +0.1 126,880 + GFF3302 0.79 -0.8 126,880 + GFF3302 0.79 +0.4 126,880 + GFF3302 0.79 -0.1 126,880 + GFF3302 0.79 -0.0 126,881 - GFF3302 0.79 -1.1 126,881 - GFF3302 0.79 -0.9 126,881 - GFF3302 0.79 -0.3 126,881 - GFF3302 0.79 -0.9 126,881 - GFF3302 0.79 +2.4 126,881 - GFF3302 0.79 -1.5 126,881 - GFF3302 0.79 +0.1 126,881 - GFF3302 0.79 -0.3 126,881 - GFF3302 0.79 -0.4 126,881 - GFF3302 0.79 -0.4 126,881 - GFF3302 0.79 -1.4 126,881 - GFF3302 0.79 -0.3 126,881 - GFF3302 0.79 -0.4 127,015 + -0.7 127,016 - -1.6 127,037 - -1.5 127,069 + -0.0 127,069 + -1.7 127,069 + -0.7 127,069 + +0.3 127,069 + +1.0 127,069 + +0.6 127,070 - +0.5 127,070 - -0.8 127,070 - -0.3 127,070 - -0.3 127,070 - -0.2 127,070 - +1.2
Or see this region's nucleotide sequence