Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF303 and GFF304 are separated by 117 nucleotides GFF304 and GFF305 are separated by 2 nucleotides GFF305 and GFF306 are separated by 55 nucleotides GFF306 and GFF307 overlap by 34 nucleotides
GFF303 - Probable transcriptional regulatory protein YebC, at 336,543 to 337,268
GFF303
GFF304 - Ubiquinone/menaquinone biosynthesis methyltransferase family protein, at 337,386 to 338,174
GFF304
GFF305 - uncharacterized domain / RidA/YER057c/UK114 superfamily, group 5, at 338,177 to 339,214
GFF305
GFF306 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 339,270 to 340,067
GFF306
GFF307 - FIG143263: Glycosyl transferase, at 340,034 to 340,810
GFF307
Position (kb) 338
339
340 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 337.241 kb on + strand at 337.241 kb on + strand at 337.241 kb on + strand at 337.241 kb on + strand at 337.242 kb on - strand at 337.242 kb on - strand at 337.242 kb on - strand at 337.256 kb on + strand at 337.256 kb on + strand at 337.256 kb on + strand at 337.256 kb on + strand at 337.257 kb on - strand at 337.257 kb on - strand at 337.257 kb on - strand at 337.257 kb on - strand at 337.257 kb on - strand at 337.276 kb on + strand at 337.276 kb on + strand at 337.277 kb on - strand at 337.315 kb on + strand at 337.315 kb on + strand at 337.315 kb on + strand at 337.315 kb on + strand at 337.316 kb on - strand at 337.316 kb on - strand at 337.316 kb on - strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.377 kb on - strand at 337.380 kb on - strand at 337.455 kb on + strand at 337.455 kb on + strand at 337.456 kb on - strand at 337.456 kb on - strand at 337.456 kb on - strand at 337.456 kb on - strand at 337.458 kb on + strand at 337.458 kb on + strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.521 kb on + strand, within GFF304 at 337.521 kb on + strand, within GFF304 at 337.522 kb on - strand, within GFF304 at 337.566 kb on + strand, within GFF304 at 337.812 kb on + strand, within GFF304 at 337.812 kb on + strand, within GFF304 at 337.812 kb on + strand, within GFF304 at 337.812 kb on + strand, within GFF304 at 337.905 kb on - strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 338.019 kb on + strand, within GFF304 at 338.019 kb on + strand, within GFF304 at 338.020 kb on - strand, within GFF304 at 338.020 kb on - strand, within GFF304 at 338.020 kb on - strand, within GFF304 at 338.023 kb on - strand, within GFF304 at 338.133 kb on + strand at 338.133 kb on + strand at 338.134 kb on - strand at 338.134 kb on - strand at 338.135 kb on + strand at 338.135 kb on + strand at 338.135 kb on + strand at 338.136 kb on - strand at 338.156 kb on + strand at 338.157 kb on - strand at 338.232 kb on - strand at 338.291 kb on + strand, within GFF305 at 338.392 kb on + strand, within GFF305 at 338.393 kb on - strand, within GFF305 at 338.393 kb on - strand, within GFF305 at 338.513 kb on + strand, within GFF305 at 338.514 kb on - strand, within GFF305 at 338.514 kb on - strand, within GFF305 at 338.514 kb on - strand, within GFF305 at 338.514 kb on - strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.766 kb on + strand, within GFF305 at 338.768 kb on + strand, within GFF305 at 338.792 kb on + strand, within GFF305 at 338.810 kb on + strand, within GFF305 at 338.810 kb on + strand, within GFF305 at 338.810 kb on + strand, within GFF305 at 338.811 kb on - strand, within GFF305 at 338.811 kb on - strand, within GFF305 at 338.811 kb on - strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.097 kb on - strand, within GFF305 at 339.131 kb on + strand at 339.131 kb on + strand at 339.132 kb on - strand at 339.132 kb on - strand at 339.132 kb on - strand at 339.322 kb on - strand at 339.384 kb on + strand, within GFF306 at 339.384 kb on + strand, within GFF306 at 339.384 kb on + strand, within GFF306 at 339.385 kb on - strand, within GFF306 at 339.444 kb on + strand, within GFF306 at 339.445 kb on - strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.865 kb on - strand, within GFF306 at 339.865 kb on - strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.988 kb on - strand at 339.988 kb on - strand at 339.988 kb on - strand at 340.076 kb on + strand at 340.076 kb on + strand at 340.076 kb on + strand at 340.076 kb on + strand at 340.077 kb on - strand at 340.077 kb on - strand at 340.077 kb on - strand at 340.077 kb on - strand at 340.137 kb on + strand, within GFF307
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 337,241 + -1.2 337,241 + -1.1 337,241 + -0.5 337,241 + -1.4 337,242 - -0.7 337,242 - +0.3 337,242 - -2.3 337,256 + -0.5 337,256 + +0.4 337,256 + +0.2 337,256 + -1.3 337,257 - -0.9 337,257 - -0.8 337,257 - -0.5 337,257 - -1.5 337,257 - -1.3 337,276 + -0.7 337,276 + -0.4 337,277 - -1.0 337,315 + -0.2 337,315 + -1.1 337,315 + -0.3 337,315 + +0.7 337,316 - +0.5 337,316 - -1.5 337,316 - -0.6 337,376 + -0.9 337,376 + +0.0 337,376 + -1.7 337,376 + -0.7 337,376 + -0.6 337,376 + +0.3 337,377 - -0.8 337,380 - -0.5 337,455 + +0.1 337,455 + -0.7 337,456 - -0.2 337,456 - -0.0 337,456 - +0.4 337,456 - -0.6 337,458 + -0.1 337,458 + -0.7 337,459 - -1.3 337,459 - -0.5 337,459 - +0.7 337,459 - -1.3 337,459 - -0.3 337,459 - -0.0 337,459 - +0.4 337,521 + GFF304 0.17 -0.7 337,521 + GFF304 0.17 +0.1 337,522 - GFF304 0.17 -0.8 337,566 + GFF304 0.23 +0.5 337,812 + GFF304 0.54 -0.8 337,812 + GFF304 0.54 +0.5 337,812 + GFF304 0.54 -0.6 337,812 + GFF304 0.54 -0.3 337,905 - GFF304 0.66 -0.3 337,947 + GFF304 0.71 -0.7 337,947 + GFF304 0.71 -0.4 337,947 + GFF304 0.71 +0.0 337,947 + GFF304 0.71 -0.2 337,947 + GFF304 0.71 +0.0 337,947 + GFF304 0.71 -0.4 337,947 + GFF304 0.71 -0.7 337,947 + GFF304 0.71 +1.1 337,947 + GFF304 0.71 -0.1 337,948 - GFF304 0.71 -1.0 337,948 - GFF304 0.71 -0.2 337,948 - GFF304 0.71 +0.4 337,948 - GFF304 0.71 -0.3 337,948 - GFF304 0.71 +0.1 337,948 - GFF304 0.71 -0.4 337,948 - GFF304 0.71 -0.9 338,019 + GFF304 0.80 -0.2 338,019 + GFF304 0.80 +0.4 338,020 - GFF304 0.80 +1.9 338,020 - GFF304 0.80 -0.5 338,020 - GFF304 0.80 -0.7 338,023 - GFF304 0.81 -0.2 338,133 + -0.2 338,133 + +0.8 338,134 - -0.5 338,134 - +0.0 338,135 + -0.4 338,135 + -0.8 338,135 + -0.2 338,136 - +1.3 338,156 + +0.3 338,157 - -0.0 338,232 - -0.3 338,291 + GFF305 0.11 -3.4 338,392 + GFF305 0.21 -1.9 338,393 - GFF305 0.21 -2.5 338,393 - GFF305 0.21 -1.8 338,513 + GFF305 0.32 -2.2 338,514 - GFF305 0.32 -0.7 338,514 - GFF305 0.32 -0.6 338,514 - GFF305 0.32 -1.0 338,514 - GFF305 0.32 -1.9 338,576 + GFF305 0.38 -0.3 338,576 + GFF305 0.38 -0.0 338,576 + GFF305 0.38 -1.9 338,576 + GFF305 0.38 -1.0 338,576 + GFF305 0.38 -0.3 338,577 - GFF305 0.39 +0.5 338,577 - GFF305 0.39 -1.5 338,577 - GFF305 0.39 -1.0 338,577 - GFF305 0.39 -1.3 338,577 - GFF305 0.39 +0.4 338,577 - GFF305 0.39 -1.2 338,766 + GFF305 0.57 -1.9 338,768 + GFF305 0.57 -0.9 338,792 + GFF305 0.59 -1.4 338,810 + GFF305 0.61 -0.7 338,810 + GFF305 0.61 -1.5 338,810 + GFF305 0.61 -2.2 338,811 - GFF305 0.61 -1.0 338,811 - GFF305 0.61 -1.3 338,811 - GFF305 0.61 -1.7 339,041 + GFF305 0.83 -1.6 339,041 + GFF305 0.83 +1.9 339,041 + GFF305 0.83 -0.0 339,041 + GFF305 0.83 -0.9 339,041 + GFF305 0.83 -1.0 339,041 + GFF305 0.83 -3.2 339,041 + GFF305 0.83 -0.6 339,041 + GFF305 0.83 +1.3 339,042 - GFF305 0.83 -1.7 339,042 - GFF305 0.83 -1.3 339,042 - GFF305 0.83 -0.7 339,042 - GFF305 0.83 -1.6 339,042 - GFF305 0.83 -0.0 339,042 - GFF305 0.83 -2.4 339,042 - GFF305 0.83 -1.6 339,042 - GFF305 0.83 -0.7 339,045 + GFF305 0.84 -1.1 339,045 + GFF305 0.84 -1.1 339,045 + GFF305 0.84 -2.0 339,045 + GFF305 0.84 +0.3 339,045 + GFF305 0.84 -1.9 339,045 + GFF305 0.84 -2.0 339,045 + GFF305 0.84 -1.8 339,045 + GFF305 0.84 -0.8 339,046 - GFF305 0.84 -0.7 339,046 - GFF305 0.84 -0.5 339,046 - GFF305 0.84 -1.8 339,046 - GFF305 0.84 +0.3 339,046 - GFF305 0.84 -1.2 339,097 - GFF305 0.89 -1.9 339,131 + -1.5 339,131 + -1.4 339,132 - -0.7 339,132 - +0.3 339,132 - -0.5 339,322 - +0.2 339,384 + GFF306 0.14 +0.5 339,384 + GFF306 0.14 +0.3 339,384 + GFF306 0.14 +0.4 339,385 - GFF306 0.14 -0.1 339,444 + GFF306 0.22 +0.1 339,445 - GFF306 0.22 -0.7 339,666 + GFF306 0.50 -1.4 339,666 + GFF306 0.50 -0.5 339,666 + GFF306 0.50 -0.3 339,666 + GFF306 0.50 -0.5 339,666 + GFF306 0.50 -0.4 339,667 - GFF306 0.50 +0.7 339,667 - GFF306 0.50 -1.6 339,667 - GFF306 0.50 -0.3 339,667 - GFF306 0.50 +0.5 339,667 - GFF306 0.50 -0.1 339,667 - GFF306 0.50 -0.5 339,667 - GFF306 0.50 -0.2 339,667 - GFF306 0.50 -0.1 339,667 - GFF306 0.50 -0.1 339,667 - GFF306 0.50 +0.7 339,865 - GFF306 0.75 -1.0 339,865 - GFF306 0.75 -0.4 339,987 + GFF306 0.90 +1.3 339,987 + GFF306 0.90 +1.9 339,987 + GFF306 0.90 +0.3 339,987 + GFF306 0.90 -0.5 339,987 + GFF306 0.90 -0.7 339,987 + GFF306 0.90 -0.4 339,987 + GFF306 0.90 +1.0 339,988 - +0.5 339,988 - -0.4 339,988 - +0.5 340,076 + -0.4 340,076 + +0.2 340,076 + +1.7 340,076 + -1.7 340,077 - -0.4 340,077 - -0.3 340,077 - +0.1 340,077 - +0.2 340,137 + GFF307 0.13 +0.1
Or see this region's nucleotide sequence