Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF290 and GFF291 overlap by 1 nucleotides GFF291 and GFF292 are separated by 25 nucleotides GFF292 and GFF293 are separated by 198 nucleotides GFF293 and GFF294 are separated by 27 nucleotides
GFF290 - Cell division coordinator CpoB, at 325,065 to 325,814
GFF290
GFF291 - Tol-Pal system peptidoglycan-associated lipoprotein PAL, at 325,814 to 326,332
GFF291
GFF292 - Tol-Pal system beta propeller repeat protein TolB, at 326,358 to 327,584
GFF292
GFF293 - no description, at 327,783 to 327,926
GFF293
GFF294 - Lipid A export permease/ATP-binding protein MsbA, at 327,954 to 329,621
GFF294
Position (kb) 326
327
328 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 325.824 kb on + strand at 325.824 kb on + strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.109 kb on + strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.110 kb on - strand, within GFF291 at 326.118 kb on + strand, within GFF291 at 326.127 kb on + strand, within GFF291 at 326.127 kb on + strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.128 kb on - strand, within GFF291 at 326.313 kb on + strand at 326.321 kb on - strand at 326.349 kb on + strand at 326.349 kb on + strand at 326.349 kb on + strand at 326.350 kb on - strand at 326.355 kb on + strand at 326.355 kb on + strand at 326.355 kb on + strand at 326.355 kb on + strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.356 kb on - strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.394 kb on + strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.395 kb on - strand at 326.485 kb on + strand, within GFF292 at 326.486 kb on - strand, within GFF292 at 326.546 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.614 kb on + strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.615 kb on - strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.659 kb on + strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.660 kb on - strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.713 kb on + strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.714 kb on - strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.749 kb on + strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.750 kb on - strand, within GFF292 at 326.977 kb on + strand, within GFF292 at 326.977 kb on + strand, within GFF292 at 326.978 kb on - strand, within GFF292 at 326.978 kb on - strand, within GFF292 at 326.978 kb on - strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.010 kb on + strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.011 kb on - strand, within GFF292 at 327.118 kb on + strand, within GFF292 at 327.142 kb on + strand, within GFF292 at 327.142 kb on + strand, within GFF292 at 327.142 kb on + strand, within GFF292 at 327.143 kb on - strand, within GFF292 at 327.190 kb on + strand, within GFF292 at 327.190 kb on + strand, within GFF292 at 327.190 kb on + strand, within GFF292 at 327.190 kb on + strand, within GFF292 at 327.190 kb on + strand, within GFF292 at 327.191 kb on - strand, within GFF292 at 327.191 kb on - strand, within GFF292 at 327.191 kb on - strand, within GFF292 at 327.191 kb on - strand, within GFF292 at 327.191 kb on - strand, within GFF292 at 327.196 kb on + strand, within GFF292 at 327.196 kb on + strand, within GFF292 at 327.196 kb on + strand, within GFF292 at 327.196 kb on + strand, within GFF292 at 327.196 kb on + strand, within GFF292 at 327.196 kb on + strand, within GFF292 at 327.197 kb on - strand, within GFF292 at 327.197 kb on - strand, within GFF292 at 327.197 kb on - strand, within GFF292 at 327.197 kb on - strand, within GFF292 at 327.247 kb on + strand, within GFF292 at 327.247 kb on + strand, within GFF292 at 327.247 kb on + strand, within GFF292 at 327.247 kb on + strand, within GFF292 at 327.247 kb on + strand, within GFF292 at 327.248 kb on - strand, within GFF292 at 327.248 kb on - strand, within GFF292 at 327.248 kb on - strand, within GFF292 at 327.248 kb on - strand, within GFF292 at 327.248 kb on - strand, within GFF292 at 327.248 kb on - strand, within GFF292 at 327.335 kb on + strand, within GFF292 at 327.423 kb on + strand, within GFF292 at 327.423 kb on + strand, within GFF292 at 327.423 kb on + strand, within GFF292 at 327.423 kb on + strand, within GFF292 at 327.627 kb on + strand at 327.627 kb on + strand at 327.627 kb on + strand at 327.627 kb on + strand at 327.627 kb on + strand at 327.628 kb on - strand at 327.628 kb on - strand at 327.628 kb on - strand at 327.628 kb on - strand at 327.731 kb on + strand at 327.731 kb on + strand at 327.731 kb on + strand at 327.731 kb on + strand at 327.731 kb on + strand at 327.731 kb on + strand at 327.731 kb on + strand at 327.731 kb on + strand at 327.732 kb on - strand at 327.732 kb on - strand at 327.732 kb on - strand at 327.732 kb on - strand at 327.767 kb on - strand at 327.784 kb on + strand at 327.785 kb on - strand at 327.785 kb on - strand at 327.785 kb on - strand at 327.823 kb on + strand, within GFF293 at 327.823 kb on + strand, within GFF293 at 327.824 kb on - strand, within GFF293 at 327.824 kb on - strand, within GFF293
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 325,824 + -0.8 325,824 + -0.5 325,825 - -1.0 325,825 - -0.7 325,825 - -0.9 325,825 - -1.3 325,992 + GFF291 0.34 -3.1 325,992 + GFF291 0.34 -1.9 325,992 + GFF291 0.34 -1.2 325,992 + GFF291 0.34 -2.0 325,993 - GFF291 0.34 -1.5 325,993 - GFF291 0.34 -1.7 325,993 - GFF291 0.34 -0.3 325,993 - GFF291 0.34 -1.5 325,993 - GFF291 0.34 -1.0 325,993 - GFF291 0.34 -1.0 325,993 - GFF291 0.34 -1.5 325,993 - GFF291 0.34 -0.7 325,993 - GFF291 0.34 +1.3 325,993 - GFF291 0.34 -0.3 325,993 - GFF291 0.34 -3.5 325,993 - GFF291 0.34 -1.9 325,993 - GFF291 0.34 -2.7 325,993 - GFF291 0.34 -1.5 326,109 + GFF291 0.57 -2.0 326,109 + GFF291 0.57 +0.5 326,109 + GFF291 0.57 -0.7 326,109 + GFF291 0.57 -0.3 326,109 + GFF291 0.57 +0.4 326,109 + GFF291 0.57 -1.5 326,109 + GFF291 0.57 -2.0 326,109 + GFF291 0.57 -1.0 326,110 - GFF291 0.57 -2.0 326,110 - GFF291 0.57 -3.2 326,110 - GFF291 0.57 -2.4 326,110 - GFF291 0.57 -0.5 326,110 - GFF291 0.57 -0.1 326,110 - GFF291 0.57 -0.9 326,110 - GFF291 0.57 -2.7 326,110 - GFF291 0.57 -1.5 326,118 + GFF291 0.59 -1.5 326,127 + GFF291 0.60 -2.3 326,127 + GFF291 0.60 -1.8 326,128 - GFF291 0.61 -1.9 326,128 - GFF291 0.61 -2.7 326,128 - GFF291 0.61 -0.1 326,128 - GFF291 0.61 -0.2 326,128 - GFF291 0.61 -4.2 326,128 - GFF291 0.61 +0.6 326,128 - GFF291 0.61 +0.3 326,313 + -0.5 326,321 - -0.3 326,349 + -0.5 326,349 + -0.7 326,349 + -0.2 326,350 - -0.1 326,355 + +0.5 326,355 + -0.2 326,355 + -1.2 326,355 + -0.3 326,356 - +0.7 326,356 - -1.5 326,356 - -0.3 326,356 - +1.3 326,356 - +0.2 326,356 - -0.5 326,356 - +0.1 326,356 - -1.2 326,356 - -0.6 326,356 - -1.1 326,356 - -0.1 326,356 - +0.3 326,394 + -0.5 326,394 + +1.9 326,394 + -0.7 326,394 + +0.3 326,394 + -1.9 326,394 + -1.2 326,394 + -2.3 326,394 + -1.4 326,394 + -0.2 326,394 + -1.5 326,394 + -2.9 326,395 - -1.0 326,395 - -1.3 326,395 - -0.7 326,395 - -1.4 326,395 - +0.1 326,395 - -1.4 326,395 - -0.9 326,395 - -1.0 326,395 - -0.7 326,395 - -0.0 326,485 + GFF292 0.10 -2.6 326,486 - GFF292 0.10 +0.3 326,546 + GFF292 0.15 -1.9 326,614 + GFF292 0.21 -1.8 326,614 + GFF292 0.21 -1.4 326,614 + GFF292 0.21 -0.2 326,614 + GFF292 0.21 -1.0 326,614 + GFF292 0.21 -0.2 326,614 + GFF292 0.21 -2.5 326,614 + GFF292 0.21 -1.6 326,614 + GFF292 0.21 -1.6 326,614 + GFF292 0.21 -2.2 326,614 + GFF292 0.21 -0.3 326,615 - GFF292 0.21 -1.5 326,615 - GFF292 0.21 -2.7 326,615 - GFF292 0.21 -2.6 326,615 - GFF292 0.21 +0.7 326,615 - GFF292 0.21 -3.6 326,615 - GFF292 0.21 -2.6 326,615 - GFF292 0.21 -2.2 326,659 + GFF292 0.25 -1.7 326,659 + GFF292 0.25 -1.6 326,659 + GFF292 0.25 -2.5 326,659 + GFF292 0.25 -3.2 326,659 + GFF292 0.25 -0.1 326,659 + GFF292 0.25 -0.8 326,659 + GFF292 0.25 -0.1 326,659 + GFF292 0.25 -1.7 326,659 + GFF292 0.25 -1.2 326,659 + GFF292 0.25 -0.3 326,659 + GFF292 0.25 -0.0 326,660 - GFF292 0.25 +0.3 326,660 - GFF292 0.25 -2.0 326,660 - GFF292 0.25 -0.8 326,660 - GFF292 0.25 -1.9 326,660 - GFF292 0.25 +0.3 326,660 - GFF292 0.25 -1.5 326,660 - GFF292 0.25 -1.7 326,713 + GFF292 0.29 -0.5 326,713 + GFF292 0.29 -1.5 326,713 + GFF292 0.29 -2.0 326,713 + GFF292 0.29 +0.7 326,713 + GFF292 0.29 -2.9 326,713 + GFF292 0.29 -1.7 326,713 + GFF292 0.29 -1.5 326,713 + GFF292 0.29 -1.9 326,714 - GFF292 0.29 -0.7 326,714 - GFF292 0.29 -0.9 326,714 - GFF292 0.29 -1.0 326,714 - GFF292 0.29 -1.6 326,714 - GFF292 0.29 -1.1 326,749 + GFF292 0.32 -0.1 326,749 + GFF292 0.32 -0.4 326,749 + GFF292 0.32 +1.9 326,749 + GFF292 0.32 -1.1 326,749 + GFF292 0.32 +1.3 326,749 + GFF292 0.32 -1.9 326,749 + GFF292 0.32 -1.3 326,749 + GFF292 0.32 -2.2 326,749 + GFF292 0.32 -2.5 326,749 + GFF292 0.32 -1.0 326,749 + GFF292 0.32 -1.3 326,749 + GFF292 0.32 -2.5 326,750 - GFF292 0.32 -1.9 326,750 - GFF292 0.32 -2.0 326,750 - GFF292 0.32 -1.5 326,750 - GFF292 0.32 -3.0 326,750 - GFF292 0.32 -1.8 326,750 - GFF292 0.32 -0.9 326,750 - GFF292 0.32 -0.5 326,750 - GFF292 0.32 -0.2 326,977 + GFF292 0.50 -1.6 326,977 + GFF292 0.50 -0.7 326,978 - GFF292 0.51 -1.0 326,978 - GFF292 0.51 -2.3 326,978 - GFF292 0.51 -1.9 327,010 + GFF292 0.53 -2.2 327,010 + GFF292 0.53 -1.8 327,010 + GFF292 0.53 +2.3 327,010 + GFF292 0.53 -1.2 327,010 + GFF292 0.53 -1.0 327,010 + GFF292 0.53 -2.2 327,010 + GFF292 0.53 -2.1 327,010 + GFF292 0.53 -0.9 327,010 + GFF292 0.53 +0.3 327,010 + GFF292 0.53 -0.8 327,010 + GFF292 0.53 -1.0 327,010 + GFF292 0.53 -1.6 327,010 + GFF292 0.53 +0.3 327,010 + GFF292 0.53 -0.7 327,011 - GFF292 0.53 -1.0 327,011 - GFF292 0.53 -0.7 327,011 - GFF292 0.53 -0.5 327,011 - GFF292 0.53 -2.0 327,011 - GFF292 0.53 -2.9 327,011 - GFF292 0.53 -2.7 327,011 - GFF292 0.53 -1.4 327,011 - GFF292 0.53 -1.9 327,011 - GFF292 0.53 +1.9 327,011 - GFF292 0.53 -0.5 327,011 - GFF292 0.53 -2.6 327,011 - GFF292 0.53 -2.0 327,011 - GFF292 0.53 -1.3 327,011 - GFF292 0.53 -1.7 327,011 - GFF292 0.53 -2.0 327,011 - GFF292 0.53 -0.9 327,011 - GFF292 0.53 -1.3 327,118 + GFF292 0.62 -1.6 327,142 + GFF292 0.64 -0.7 327,142 + GFF292 0.64 -0.3 327,142 + GFF292 0.64 -1.9 327,143 - GFF292 0.64 -2.0 327,190 + GFF292 0.68 +0.7 327,190 + GFF292 0.68 -0.3 327,190 + GFF292 0.68 -1.7 327,190 + GFF292 0.68 -1.9 327,190 + GFF292 0.68 -1.5 327,191 - GFF292 0.68 -0.4 327,191 - GFF292 0.68 -0.8 327,191 - GFF292 0.68 -1.4 327,191 - GFF292 0.68 +0.1 327,191 - GFF292 0.68 -2.3 327,196 + GFF292 0.68 -1.7 327,196 + GFF292 0.68 -2.3 327,196 + GFF292 0.68 -1.9 327,196 + GFF292 0.68 -1.5 327,196 + GFF292 0.68 -1.4 327,196 + GFF292 0.68 -0.8 327,197 - GFF292 0.68 +0.9 327,197 - GFF292 0.68 +2.1 327,197 - GFF292 0.68 -1.9 327,197 - GFF292 0.68 -0.3 327,247 + GFF292 0.72 -0.9 327,247 + GFF292 0.72 +0.4 327,247 + GFF292 0.72 -1.0 327,247 + GFF292 0.72 -1.9 327,247 + GFF292 0.72 -0.5 327,248 - GFF292 0.73 -0.7 327,248 - GFF292 0.73 -0.7 327,248 - GFF292 0.73 +0.2 327,248 - GFF292 0.73 +1.3 327,248 - GFF292 0.73 -1.6 327,248 - GFF292 0.73 +0.4 327,335 + GFF292 0.80 -1.9 327,423 + GFF292 0.87 -1.5 327,423 + GFF292 0.87 -1.3 327,423 + GFF292 0.87 -1.2 327,423 + GFF292 0.87 -0.3 327,627 + -0.2 327,627 + +1.7 327,627 + -0.6 327,627 + +0.3 327,627 + +0.4 327,628 - -2.2 327,628 - -0.6 327,628 - -1.2 327,628 - +0.5 327,731 + -0.1 327,731 + -0.8 327,731 + +1.6 327,731 + +0.4 327,731 + -0.2 327,731 + -0.2 327,731 + -0.2 327,731 + -0.3 327,732 - +1.7 327,732 - -0.2 327,732 - -0.6 327,732 - +0.0 327,767 - +0.1 327,784 + -0.4 327,785 - -0.5 327,785 - -0.8 327,785 - -1.6 327,823 + GFF293 0.28 +0.3 327,823 + GFF293 0.28 +0.5 327,824 - GFF293 0.28 +0.0 327,824 - GFF293 0.28 +0.3
Or see this region's nucleotide sequence