Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2912 and GFF2913 overlap by 4 nucleotides GFF2913 and GFF2914 overlap by 17 nucleotides GFF2914 and GFF2915 are separated by 56 nucleotides GFF2915 and GFF2916 are separated by 14 nucleotides
GFF2912 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 339,993 to 340,742
GFF2912
GFF2913 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 340,739 to 341,395
GFF2913
GFF2914 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 341,379 to 342,107
GFF2914
GFF2915 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 342,164 to 342,958
GFF2915
GFF2916 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 342,973 to 343,794
GFF2916
Position (kb) 341
342
343 Strain fitness (log2 ratio) -2
-1
0
1
2 at 340.434 kb on + strand, within GFF2912 at 340.434 kb on + strand, within GFF2912 at 340.435 kb on - strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.564 kb on - strand, within GFF2912 at 340.564 kb on - strand, within GFF2912 at 340.584 kb on + strand, within GFF2912 at 340.585 kb on - strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.775 kb on + strand at 340.775 kb on + strand at 340.775 kb on + strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.907 kb on + strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.958 kb on + strand, within GFF2913 at 340.959 kb on - strand, within GFF2913 at 341.027 kb on + strand, within GFF2913 at 341.027 kb on + strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.321 kb on + strand, within GFF2913 at 341.322 kb on - strand, within GFF2913 at 341.410 kb on - strand at 341.411 kb on + strand at 341.411 kb on + strand at 341.411 kb on + strand at 341.412 kb on - strand at 341.412 kb on - strand at 341.412 kb on - strand at 341.643 kb on + strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.010 kb on - strand, within GFF2914 at 342.105 kb on + strand at 342.106 kb on - strand at 342.141 kb on - strand at 342.669 kb on - strand, within GFF2915 at 342.908 kb on + strand at 342.908 kb on + strand at 342.909 kb on - strand at 342.917 kb on + strand at 342.917 kb on + strand at 342.918 kb on - strand at 342.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 340,434 + GFF2912 0.59 -0.4 340,434 + GFF2912 0.59 +0.0 340,435 - GFF2912 0.59 +0.6 340,563 + GFF2912 0.76 -2.6 340,563 + GFF2912 0.76 +1.3 340,563 + GFF2912 0.76 +0.2 340,563 + GFF2912 0.76 -1.3 340,563 + GFF2912 0.76 -0.8 340,563 + GFF2912 0.76 -0.6 340,564 - GFF2912 0.76 -0.7 340,564 - GFF2912 0.76 +0.0 340,584 + GFF2912 0.79 -0.3 340,585 - GFF2912 0.79 -0.0 340,635 + GFF2912 0.86 -1.3 340,635 + GFF2912 0.86 -0.5 340,635 + GFF2912 0.86 -0.4 340,635 + GFF2912 0.86 -0.2 340,635 + GFF2912 0.86 +0.3 340,775 + -0.1 340,775 + -0.1 340,775 + +0.0 340,776 - +2.0 340,776 - -0.4 340,776 - +0.0 340,776 - +0.3 340,776 - +1.6 340,907 + GFF2913 0.26 +1.1 340,908 - GFF2913 0.26 -2.2 340,908 - GFF2913 0.26 -2.0 340,908 - GFF2913 0.26 +0.0 340,908 - GFF2913 0.26 +0.0 340,958 + GFF2913 0.33 -0.4 340,959 - GFF2913 0.33 +1.6 341,027 + GFF2913 0.44 -1.0 341,027 + GFF2913 0.44 -0.1 341,028 - GFF2913 0.44 +0.5 341,028 - GFF2913 0.44 +0.5 341,028 - GFF2913 0.44 -1.1 341,321 + GFF2913 0.89 -0.5 341,322 - GFF2913 0.89 +0.3 341,410 - +0.6 341,411 + +2.2 341,411 + +0.1 341,411 + -0.4 341,412 - -0.7 341,412 - +0.1 341,412 - -0.2 341,643 + GFF2914 0.36 -1.3 341,644 - GFF2914 0.36 +0.6 341,644 - GFF2914 0.36 -0.3 341,644 - GFF2914 0.36 -1.6 341,644 - GFF2914 0.36 +1.9 341,644 - GFF2914 0.36 -0.4 341,644 - GFF2914 0.36 +0.3 342,009 + GFF2914 0.86 +1.3 342,009 + GFF2914 0.86 +0.5 342,009 + GFF2914 0.86 +0.6 342,009 + GFF2914 0.86 +0.6 342,009 + GFF2914 0.86 -1.6 342,010 - GFF2914 0.87 +1.9 342,105 + -0.3 342,106 - -0.5 342,141 - -0.9 342,669 - GFF2915 0.64 +1.0 342,908 + -1.5 342,908 + -0.0 342,909 - -0.3 342,917 + -0.2 342,917 + -1.7 342,918 - +0.6 342,918 - -0.7
Or see this region's nucleotide sequence