Strain Fitness in Variovorax sp. SCN45 around GFF290

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF289 and GFF290 are separated by 19 nucleotidesGFF290 and GFF291 overlap by 1 nucleotidesGFF291 and GFF292 are separated by 25 nucleotides GFF289 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1, at 324,200 to 325,045 GFF289 GFF290 - Cell division coordinator CpoB, at 325,065 to 325,814 GFF290 GFF291 - Tol-Pal system peptidoglycan-associated lipoprotein PAL, at 325,814 to 326,332 GFF291 GFF292 - Tol-Pal system beta propeller repeat protein TolB, at 326,358 to 327,584 GFF292 Position (kb) 325 326Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 324.079 kb on - strandat 324.079 kb on - strandat 324.079 kb on - strandat 324.079 kb on - strandat 324.196 kb on - strandat 324.204 kb on - strandat 324.204 kb on - strandat 324.204 kb on - strandat 324.578 kb on + strand, within GFF289at 324.578 kb on + strand, within GFF289at 324.578 kb on + strand, within GFF289at 324.578 kb on + strand, within GFF289at 324.579 kb on - strand, within GFF289at 324.579 kb on - strand, within GFF289at 324.579 kb on - strand, within GFF289at 324.579 kb on - strand, within GFF289at 324.579 kb on - strand, within GFF289at 324.933 kb on + strand, within GFF289at 324.933 kb on + strand, within GFF289at 324.933 kb on + strand, within GFF289at 324.933 kb on + strand, within GFF289at 324.933 kb on + strand, within GFF289at 324.933 kb on + strand, within GFF289at 324.934 kb on - strand, within GFF289at 324.934 kb on - strand, within GFF289at 324.934 kb on - strand, within GFF289at 324.966 kb on + strandat 324.966 kb on + strandat 324.966 kb on + strandat 324.966 kb on + strandat 324.966 kb on + strandat 324.966 kb on + strandat 324.967 kb on - strandat 324.967 kb on - strandat 324.967 kb on - strandat 324.967 kb on - strandat 324.967 kb on - strandat 325.120 kb on + strandat 325.121 kb on - strandat 325.273 kb on + strand, within GFF290at 325.273 kb on + strand, within GFF290at 325.273 kb on + strand, within GFF290at 325.273 kb on + strand, within GFF290at 325.273 kb on + strand, within GFF290at 325.273 kb on + strand, within GFF290at 325.273 kb on + strand, within GFF290at 325.273 kb on + strand, within GFF290at 325.273 kb on + strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.274 kb on - strand, within GFF290at 325.288 kb on + strand, within GFF290at 325.289 kb on - strand, within GFF290at 325.327 kb on + strand, within GFF290at 325.327 kb on + strand, within GFF290at 325.327 kb on + strand, within GFF290at 325.327 kb on + strand, within GFF290at 325.328 kb on - strand, within GFF290at 325.328 kb on - strand, within GFF290at 325.342 kb on + strand, within GFF290at 325.824 kb on + strandat 325.824 kb on + strandat 325.825 kb on - strandat 325.825 kb on - strandat 325.825 kb on - strandat 325.825 kb on - strandat 325.992 kb on + strand, within GFF291at 325.992 kb on + strand, within GFF291at 325.992 kb on + strand, within GFF291at 325.992 kb on + strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 325.993 kb on - strand, within GFF291at 326.109 kb on + strand, within GFF291at 326.109 kb on + strand, within GFF291at 326.109 kb on + strand, within GFF291at 326.109 kb on + strand, within GFF291at 326.109 kb on + strand, within GFF291at 326.109 kb on + strand, within GFF291at 326.109 kb on + strand, within GFF291at 326.109 kb on + strand, within GFF291at 326.110 kb on - strand, within GFF291at 326.110 kb on - strand, within GFF291at 326.110 kb on - strand, within GFF291at 326.110 kb on - strand, within GFF291at 326.110 kb on - strand, within GFF291at 326.110 kb on - strand, within GFF291at 326.110 kb on - strand, within GFF291at 326.110 kb on - strand, within GFF291at 326.118 kb on + strand, within GFF291at 326.127 kb on + strand, within GFF291at 326.127 kb on + strand, within GFF291at 326.128 kb on - strand, within GFF291at 326.128 kb on - strand, within GFF291at 326.128 kb on - strand, within GFF291at 326.128 kb on - strand, within GFF291at 326.128 kb on - strand, within GFF291at 326.128 kb on - strand, within GFF291at 326.128 kb on - strand, within GFF291at 326.313 kb on + strandat 326.321 kb on - strandat 326.349 kb on + strandat 326.349 kb on + strandat 326.349 kb on + strandat 326.350 kb on - strandat 326.355 kb on + strandat 326.355 kb on + strandat 326.355 kb on + strandat 326.355 kb on + strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.356 kb on - strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.394 kb on + strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.395 kb on - strandat 326.485 kb on + strand, within GFF292at 326.486 kb on - strand, within GFF292at 326.546 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.614 kb on + strand, within GFF292at 326.615 kb on - strand, within GFF292at 326.615 kb on - strand, within GFF292at 326.615 kb on - strand, within GFF292at 326.615 kb on - strand, within GFF292at 326.615 kb on - strand, within GFF292at 326.615 kb on - strand, within GFF292at 326.615 kb on - strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.659 kb on + strand, within GFF292at 326.660 kb on - strand, within GFF292at 326.660 kb on - strand, within GFF292at 326.660 kb on - strand, within GFF292at 326.660 kb on - strand, within GFF292at 326.660 kb on - strand, within GFF292at 326.660 kb on - strand, within GFF292at 326.660 kb on - strand, within GFF292at 326.713 kb on + strand, within GFF292at 326.713 kb on + strand, within GFF292at 326.713 kb on + strand, within GFF292at 326.713 kb on + strand, within GFF292at 326.713 kb on + strand, within GFF292at 326.713 kb on + strand, within GFF292at 326.713 kb on + strand, within GFF292at 326.713 kb on + strand, within GFF292at 326.714 kb on - strand, within GFF292at 326.714 kb on - strand, within GFF292at 326.714 kb on - strand, within GFF292at 326.714 kb on - strand, within GFF292at 326.714 kb on - strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.749 kb on + strand, within GFF292at 326.750 kb on - strand, within GFF292at 326.750 kb on - strand, within GFF292at 326.750 kb on - strand, within GFF292at 326.750 kb on - strand, within GFF292at 326.750 kb on - strand, within GFF292at 326.750 kb on - strand, within GFF292at 326.750 kb on - strand, within GFF292at 326.750 kb on - strand, within GFF292

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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324,079 - +0.3
324,079 - +0.1
324,079 - -0.5
324,079 - -0.5
324,196 - -0.7
324,204 - +0.4
324,204 - +0.3
324,204 - -1.1
324,578 + GFF289 0.45 -0.9
324,578 + GFF289 0.45 -0.2
324,578 + GFF289 0.45 -0.1
324,578 + GFF289 0.45 +0.0
324,579 - GFF289 0.45 -0.4
324,579 - GFF289 0.45 +0.3
324,579 - GFF289 0.45 -0.3
324,579 - GFF289 0.45 +0.3
324,579 - GFF289 0.45 -1.4
324,933 + GFF289 0.87 -0.7
324,933 + GFF289 0.87 +0.2
324,933 + GFF289 0.87 -0.1
324,933 + GFF289 0.87 -1.1
324,933 + GFF289 0.87 +0.8
324,933 + GFF289 0.87 -0.5
324,934 - GFF289 0.87 +0.3
324,934 - GFF289 0.87 -1.1
324,934 - GFF289 0.87 -0.6
324,966 + -0.5
324,966 + -0.2
324,966 + -0.2
324,966 + +1.9
324,966 + -1.9
324,966 + -0.4
324,967 - -0.5
324,967 - -0.2
324,967 - -1.6
324,967 - -0.1
324,967 - -0.6
325,120 + +0.3
325,121 - -1.2
325,273 + GFF290 0.28 -1.1
325,273 + GFF290 0.28 -0.6
325,273 + GFF290 0.28 +0.0
325,273 + GFF290 0.28 +1.3
325,273 + GFF290 0.28 -2.2
325,273 + GFF290 0.28 +0.3
325,273 + GFF290 0.28 +2.3
325,273 + GFF290 0.28 -1.3
325,273 + GFF290 0.28 -2.0
325,274 - GFF290 0.28 -1.4
325,274 - GFF290 0.28 -0.3
325,274 - GFF290 0.28 -0.3
325,274 - GFF290 0.28 -1.3
325,274 - GFF290 0.28 -0.5
325,274 - GFF290 0.28 -2.4
325,274 - GFF290 0.28 -1.9
325,274 - GFF290 0.28 -1.0
325,274 - GFF290 0.28 -0.4
325,274 - GFF290 0.28 -0.3
325,274 - GFF290 0.28 -0.3
325,288 + GFF290 0.30 -0.4
325,289 - GFF290 0.30 -0.7
325,327 + GFF290 0.35 -1.7
325,327 + GFF290 0.35 -2.4
325,327 + GFF290 0.35 -1.3
325,327 + GFF290 0.35 -0.7
325,328 - GFF290 0.35 -1.5
325,328 - GFF290 0.35 -0.9
325,342 + GFF290 0.37 -0.7
325,824 + -0.8
325,824 + -0.5
325,825 - -1.0
325,825 - -0.7
325,825 - -0.9
325,825 - -1.3
325,992 + GFF291 0.34 -3.1
325,992 + GFF291 0.34 -1.9
325,992 + GFF291 0.34 -1.2
325,992 + GFF291 0.34 -2.0
325,993 - GFF291 0.34 -1.5
325,993 - GFF291 0.34 -1.7
325,993 - GFF291 0.34 -0.3
325,993 - GFF291 0.34 -1.5
325,993 - GFF291 0.34 -1.0
325,993 - GFF291 0.34 -1.0
325,993 - GFF291 0.34 -1.5
325,993 - GFF291 0.34 -0.7
325,993 - GFF291 0.34 +1.3
325,993 - GFF291 0.34 -0.3
325,993 - GFF291 0.34 -3.5
325,993 - GFF291 0.34 -1.9
325,993 - GFF291 0.34 -2.7
325,993 - GFF291 0.34 -1.5
326,109 + GFF291 0.57 -2.0
326,109 + GFF291 0.57 +0.5
326,109 + GFF291 0.57 -0.7
326,109 + GFF291 0.57 -0.3
326,109 + GFF291 0.57 +0.4
326,109 + GFF291 0.57 -1.5
326,109 + GFF291 0.57 -2.0
326,109 + GFF291 0.57 -1.0
326,110 - GFF291 0.57 -2.0
326,110 - GFF291 0.57 -3.2
326,110 - GFF291 0.57 -2.4
326,110 - GFF291 0.57 -0.5
326,110 - GFF291 0.57 -0.1
326,110 - GFF291 0.57 -0.9
326,110 - GFF291 0.57 -2.7
326,110 - GFF291 0.57 -1.5
326,118 + GFF291 0.59 -1.5
326,127 + GFF291 0.60 -2.3
326,127 + GFF291 0.60 -1.8
326,128 - GFF291 0.61 -1.9
326,128 - GFF291 0.61 -2.7
326,128 - GFF291 0.61 -0.1
326,128 - GFF291 0.61 -0.2
326,128 - GFF291 0.61 -4.2
326,128 - GFF291 0.61 +0.6
326,128 - GFF291 0.61 +0.3
326,313 + -0.5
326,321 - -0.3
326,349 + -0.5
326,349 + -0.7
326,349 + -0.2
326,350 - -0.1
326,355 + +0.5
326,355 + -0.2
326,355 + -1.2
326,355 + -0.3
326,356 - +0.7
326,356 - -1.5
326,356 - -0.3
326,356 - +1.3
326,356 - +0.2
326,356 - -0.5
326,356 - +0.1
326,356 - -1.2
326,356 - -0.6
326,356 - -1.1
326,356 - -0.1
326,356 - +0.3
326,394 + -0.5
326,394 + +1.9
326,394 + -0.7
326,394 + +0.3
326,394 + -1.9
326,394 + -1.2
326,394 + -2.3
326,394 + -1.4
326,394 + -0.2
326,394 + -1.5
326,394 + -2.9
326,395 - -1.0
326,395 - -1.3
326,395 - -0.7
326,395 - -1.4
326,395 - +0.1
326,395 - -1.4
326,395 - -0.9
326,395 - -1.0
326,395 - -0.7
326,395 - -0.0
326,485 + GFF292 0.10 -2.6
326,486 - GFF292 0.10 +0.3
326,546 + GFF292 0.15 -1.9
326,614 + GFF292 0.21 -1.8
326,614 + GFF292 0.21 -1.4
326,614 + GFF292 0.21 -0.2
326,614 + GFF292 0.21 -1.0
326,614 + GFF292 0.21 -0.2
326,614 + GFF292 0.21 -2.5
326,614 + GFF292 0.21 -1.6
326,614 + GFF292 0.21 -1.6
326,614 + GFF292 0.21 -2.2
326,614 + GFF292 0.21 -0.3
326,615 - GFF292 0.21 -1.5
326,615 - GFF292 0.21 -2.7
326,615 - GFF292 0.21 -2.6
326,615 - GFF292 0.21 +0.7
326,615 - GFF292 0.21 -3.6
326,615 - GFF292 0.21 -2.6
326,615 - GFF292 0.21 -2.2
326,659 + GFF292 0.25 -1.7
326,659 + GFF292 0.25 -1.6
326,659 + GFF292 0.25 -2.5
326,659 + GFF292 0.25 -3.2
326,659 + GFF292 0.25 -0.1
326,659 + GFF292 0.25 -0.8
326,659 + GFF292 0.25 -0.1
326,659 + GFF292 0.25 -1.7
326,659 + GFF292 0.25 -1.2
326,659 + GFF292 0.25 -0.3
326,659 + GFF292 0.25 -0.0
326,660 - GFF292 0.25 +0.3
326,660 - GFF292 0.25 -2.0
326,660 - GFF292 0.25 -0.8
326,660 - GFF292 0.25 -1.9
326,660 - GFF292 0.25 +0.3
326,660 - GFF292 0.25 -1.5
326,660 - GFF292 0.25 -1.7
326,713 + GFF292 0.29 -0.5
326,713 + GFF292 0.29 -1.5
326,713 + GFF292 0.29 -2.0
326,713 + GFF292 0.29 +0.7
326,713 + GFF292 0.29 -2.9
326,713 + GFF292 0.29 -1.7
326,713 + GFF292 0.29 -1.5
326,713 + GFF292 0.29 -1.9
326,714 - GFF292 0.29 -0.7
326,714 - GFF292 0.29 -0.9
326,714 - GFF292 0.29 -1.0
326,714 - GFF292 0.29 -1.6
326,714 - GFF292 0.29 -1.1
326,749 + GFF292 0.32 -0.1
326,749 + GFF292 0.32 -0.4
326,749 + GFF292 0.32 +1.9
326,749 + GFF292 0.32 -1.1
326,749 + GFF292 0.32 +1.3
326,749 + GFF292 0.32 -1.9
326,749 + GFF292 0.32 -1.3
326,749 + GFF292 0.32 -2.2
326,749 + GFF292 0.32 -2.5
326,749 + GFF292 0.32 -1.0
326,749 + GFF292 0.32 -1.3
326,749 + GFF292 0.32 -2.5
326,750 - GFF292 0.32 -1.9
326,750 - GFF292 0.32 -2.0
326,750 - GFF292 0.32 -1.5
326,750 - GFF292 0.32 -3.0
326,750 - GFF292 0.32 -1.8
326,750 - GFF292 0.32 -0.9
326,750 - GFF292 0.32 -0.5
326,750 - GFF292 0.32 -0.2

Or see this region's nucleotide sequence