Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2836 and GFF2837 are separated by 5 nucleotides GFF2837 and GFF2838 are separated by 94 nucleotides GFF2838 and GFF2839 are separated by 183 nucleotides GFF2839 and GFF2840 are separated by 193 nucleotides GFF2840 and GFF2841 are separated by 59 nucleotides
GFF2836 - MotA/TolQ/ExbB proton channel family protein, at 262,643 to 263,356
GFF2836
GFF2837 - Biopolymer transport protein ExbD/TolR, at 263,362 to 263,775
GFF2837
GFF2838 - hypothetical protein, at 263,870 to 264,082
GFF2838
GFF2839 - Protein GlcG, at 264,266 to 264,673
GFF2839
GFF2840 - Integral membrane protein, interacts with FtsH, at 264,867 to 265,568
GFF2840
GFF2841 - 23S rRNA (uracil(1939)-C(5))-methyltransferase (EC 2.1.1.190), at 265,628 to 267,064
GFF2841
Position (kb) 264
265 Strain fitness (log2 ratio) -2
-1
0
1 at 263.288 kb on + strand at 263.288 kb on + strand at 263.288 kb on + strand at 263.289 kb on - strand at 263.289 kb on - strand at 263.575 kb on + strand, within GFF2837 at 263.576 kb on - strand, within GFF2837 at 263.578 kb on + strand, within GFF2837 at 263.578 kb on + strand, within GFF2837 at 263.767 kb on - strand at 263.856 kb on + strand at 264.036 kb on - strand, within GFF2838 at 264.036 kb on - strand, within GFF2838 at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.080 kb on + strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.081 kb on - strand at 264.179 kb on + strand at 264.180 kb on - strand at 264.180 kb on - strand at 264.424 kb on + strand, within GFF2839 at 264.425 kb on - strand, within GFF2839 at 264.425 kb on - strand, within GFF2839 at 264.450 kb on + strand, within GFF2839 at 264.450 kb on + strand, within GFF2839 at 264.450 kb on + strand, within GFF2839 at 264.450 kb on + strand, within GFF2839 at 264.450 kb on + strand, within GFF2839 at 264.450 kb on + strand, within GFF2839 at 264.451 kb on - strand, within GFF2839 at 264.451 kb on - strand, within GFF2839 at 264.451 kb on - strand, within GFF2839 at 264.451 kb on - strand, within GFF2839 at 264.583 kb on + strand, within GFF2839 at 264.584 kb on - strand, within GFF2839 at 264.692 kb on + strand at 264.693 kb on - strand at 264.693 kb on - strand at 264.751 kb on + strand at 264.751 kb on + strand at 264.751 kb on + strand at 264.751 kb on + strand at 264.751 kb on + strand at 264.751 kb on + strand at 264.752 kb on - strand at 264.752 kb on - strand at 264.752 kb on - strand at 264.752 kb on - strand at 264.752 kb on - strand at 264.752 kb on - strand at 264.767 kb on + strand at 264.767 kb on + strand at 264.767 kb on + strand at 264.767 kb on + strand at 264.767 kb on + strand at 264.767 kb on + strand at 264.767 kb on + strand at 264.768 kb on - strand at 264.768 kb on - strand at 264.768 kb on - strand at 264.768 kb on - strand at 264.768 kb on - strand at 264.768 kb on - strand at 264.783 kb on + strand at 264.783 kb on + strand at 264.783 kb on + strand at 264.783 kb on + strand at 264.783 kb on + strand at 264.783 kb on + strand at 264.784 kb on - strand at 264.784 kb on - strand at 264.784 kb on - strand at 264.784 kb on - strand at 264.784 kb on - strand at 264.818 kb on + strand at 264.818 kb on + strand at 264.818 kb on + strand at 264.819 kb on - strand at 264.819 kb on - strand at 264.819 kb on - strand at 264.841 kb on + strand at 264.841 kb on + strand at 264.841 kb on + strand at 264.841 kb on + strand at 264.841 kb on + strand at 264.841 kb on + strand at 264.842 kb on - strand at 264.842 kb on - strand at 264.842 kb on - strand at 264.882 kb on - strand at 264.889 kb on + strand at 264.889 kb on + strand at 264.889 kb on + strand at 264.889 kb on + strand at 264.889 kb on + strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.890 kb on - strand at 264.906 kb on + strand at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.957 kb on + strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 264.958 kb on - strand, within GFF2840 at 265.102 kb on - strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.425 kb on + strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.426 kb on - strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.434 kb on + strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.435 kb on - strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.473 kb on + strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.474 kb on - strand, within GFF2840 at 265.497 kb on + strand, within GFF2840 at 265.497 kb on + strand, within GFF2840 at 265.497 kb on + strand, within GFF2840 at 265.497 kb on + strand, within GFF2840 at 265.497 kb on + strand, within GFF2840 at 265.498 kb on - strand at 265.498 kb on - strand at 265.498 kb on - strand at 265.498 kb on - strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.603 kb on + strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand at 265.604 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 263,288 + -1.4 263,288 + -1.2 263,288 + +0.3 263,289 - -1.6 263,289 - +0.6 263,575 + GFF2837 0.51 -1.0 263,576 - GFF2837 0.52 -1.2 263,578 + GFF2837 0.52 +0.3 263,578 + GFF2837 0.52 -0.9 263,767 - -0.4 263,856 + -0.6 264,036 - GFF2838 0.78 -0.6 264,036 - GFF2838 0.78 -0.4 264,080 + +0.0 264,080 + -0.3 264,080 + -1.0 264,080 + -0.7 264,080 + -0.3 264,080 + -1.9 264,080 + -0.1 264,080 + +0.6 264,080 + +1.6 264,080 + -0.4 264,080 + -0.7 264,080 + +0.5 264,081 - -0.5 264,081 - -0.4 264,081 - +0.3 264,081 - -0.4 264,081 - -1.4 264,081 - +0.4 264,081 - -0.4 264,081 - -0.8 264,081 - -0.6 264,081 - -2.4 264,081 - -0.6 264,081 - -1.6 264,179 + -0.8 264,180 - +1.4 264,180 - -0.3 264,424 + GFF2839 0.39 -0.4 264,425 - GFF2839 0.39 +0.3 264,425 - GFF2839 0.39 +1.6 264,450 + GFF2839 0.45 -0.2 264,450 + GFF2839 0.45 +0.0 264,450 + GFF2839 0.45 -0.5 264,450 + GFF2839 0.45 -1.2 264,450 + GFF2839 0.45 +1.2 264,450 + GFF2839 0.45 -0.5 264,451 - GFF2839 0.45 -1.1 264,451 - GFF2839 0.45 +0.3 264,451 - GFF2839 0.45 -0.6 264,451 - GFF2839 0.45 -0.2 264,583 + GFF2839 0.78 +0.0 264,584 - GFF2839 0.78 -0.4 264,692 + -0.3 264,693 - -1.1 264,693 - -0.4 264,751 + -1.3 264,751 + +0.1 264,751 + -0.2 264,751 + -1.4 264,751 + -0.4 264,751 + -0.7 264,752 - -0.3 264,752 - +0.0 264,752 - -0.4 264,752 - +1.0 264,752 - +0.9 264,752 - -0.9 264,767 + -0.7 264,767 + -1.0 264,767 + -0.4 264,767 + +0.0 264,767 + -0.4 264,767 + +0.4 264,767 + -0.8 264,768 - -0.3 264,768 - -0.3 264,768 - -0.9 264,768 - -1.0 264,768 - -0.9 264,768 - +0.0 264,783 + +0.0 264,783 + -1.1 264,783 + -0.1 264,783 + +0.0 264,783 + +0.4 264,783 + +0.7 264,784 - -0.1 264,784 - +0.2 264,784 - -0.6 264,784 - +0.6 264,784 - -0.3 264,818 + -0.3 264,818 + +0.4 264,818 + +0.2 264,819 - -0.8 264,819 - -0.3 264,819 - +1.7 264,841 + -0.5 264,841 + -0.7 264,841 + +1.4 264,841 + +0.0 264,841 + -0.3 264,841 + -0.1 264,842 - -0.2 264,842 - +0.2 264,842 - -0.7 264,882 - -1.1 264,889 + -1.9 264,889 + +0.6 264,889 + -0.0 264,889 + -0.3 264,889 + +0.6 264,890 - +0.2 264,890 - +0.1 264,890 - -0.6 264,890 - -0.7 264,890 - +0.2 264,890 - -1.2 264,890 - -0.3 264,890 - -0.4 264,890 - -0.4 264,890 - -0.2 264,890 - +0.7 264,906 + -0.3 264,957 + GFF2840 0.13 -0.4 264,957 + GFF2840 0.13 -0.8 264,957 + GFF2840 0.13 +0.4 264,957 + GFF2840 0.13 -0.4 264,957 + GFF2840 0.13 +0.8 264,957 + GFF2840 0.13 -1.9 264,957 + GFF2840 0.13 -0.0 264,957 + GFF2840 0.13 +0.6 264,957 + GFF2840 0.13 -1.6 264,957 + GFF2840 0.13 +0.2 264,958 - GFF2840 0.13 +1.2 264,958 - GFF2840 0.13 +0.9 264,958 - GFF2840 0.13 +0.6 264,958 - GFF2840 0.13 -0.5 264,958 - GFF2840 0.13 -0.4 264,958 - GFF2840 0.13 -2.1 264,958 - GFF2840 0.13 -2.0 264,958 - GFF2840 0.13 -1.4 264,958 - GFF2840 0.13 -1.2 264,958 - GFF2840 0.13 -0.3 264,958 - GFF2840 0.13 -0.8 264,958 - GFF2840 0.13 +0.6 264,958 - GFF2840 0.13 -0.7 264,958 - GFF2840 0.13 -0.5 264,958 - GFF2840 0.13 -0.4 264,958 - GFF2840 0.13 -0.5 264,958 - GFF2840 0.13 -1.6 264,958 - GFF2840 0.13 -0.7 265,102 - GFF2840 0.33 +0.4 265,425 + GFF2840 0.79 -1.1 265,425 + GFF2840 0.79 -0.9 265,425 + GFF2840 0.79 -0.6 265,425 + GFF2840 0.79 -0.6 265,425 + GFF2840 0.79 +1.6 265,425 + GFF2840 0.79 +0.3 265,425 + GFF2840 0.79 -2.3 265,425 + GFF2840 0.79 -0.2 265,425 + GFF2840 0.79 -0.5 265,425 + GFF2840 0.79 -1.0 265,425 + GFF2840 0.79 -0.9 265,426 - GFF2840 0.80 +1.2 265,426 - GFF2840 0.80 +1.0 265,426 - GFF2840 0.80 -0.1 265,426 - GFF2840 0.80 -0.9 265,426 - GFF2840 0.80 +0.9 265,426 - GFF2840 0.80 +0.0 265,426 - GFF2840 0.80 -0.7 265,426 - GFF2840 0.80 -0.8 265,426 - GFF2840 0.80 +1.0 265,426 - GFF2840 0.80 -0.1 265,426 - GFF2840 0.80 -0.8 265,426 - GFF2840 0.80 +0.3 265,426 - GFF2840 0.80 -0.8 265,426 - GFF2840 0.80 +0.3 265,426 - GFF2840 0.80 -0.1 265,434 + GFF2840 0.81 -0.3 265,434 + GFF2840 0.81 -0.3 265,434 + GFF2840 0.81 -0.4 265,434 + GFF2840 0.81 -1.0 265,434 + GFF2840 0.81 -0.2 265,434 + GFF2840 0.81 -0.9 265,434 + GFF2840 0.81 -0.2 265,434 + GFF2840 0.81 +1.1 265,434 + GFF2840 0.81 -0.2 265,434 + GFF2840 0.81 -0.4 265,434 + GFF2840 0.81 +0.1 265,434 + GFF2840 0.81 -0.2 265,434 + GFF2840 0.81 -0.6 265,434 + GFF2840 0.81 -0.4 265,434 + GFF2840 0.81 -0.3 265,434 + GFF2840 0.81 -0.0 265,435 - GFF2840 0.81 +0.7 265,435 - GFF2840 0.81 -1.2 265,435 - GFF2840 0.81 -0.5 265,435 - GFF2840 0.81 +1.6 265,435 - GFF2840 0.81 +0.4 265,435 - GFF2840 0.81 -1.1 265,435 - GFF2840 0.81 -1.3 265,435 - GFF2840 0.81 -1.2 265,435 - GFF2840 0.81 -1.7 265,435 - GFF2840 0.81 -0.1 265,435 - GFF2840 0.81 -0.2 265,435 - GFF2840 0.81 +0.6 265,435 - GFF2840 0.81 -2.3 265,473 + GFF2840 0.86 -0.1 265,473 + GFF2840 0.86 -0.5 265,473 + GFF2840 0.86 -0.3 265,473 + GFF2840 0.86 +1.1 265,473 + GFF2840 0.86 -0.8 265,473 + GFF2840 0.86 -0.4 265,473 + GFF2840 0.86 -1.0 265,473 + GFF2840 0.86 -0.0 265,473 + GFF2840 0.86 -1.1 265,473 + GFF2840 0.86 +0.5 265,473 + GFF2840 0.86 -0.8 265,473 + GFF2840 0.86 -0.4 265,473 + GFF2840 0.86 -0.3 265,474 - GFF2840 0.86 -0.5 265,474 - GFF2840 0.86 -0.3 265,474 - GFF2840 0.86 -0.1 265,474 - GFF2840 0.86 -0.7 265,474 - GFF2840 0.86 -1.4 265,474 - GFF2840 0.86 -0.1 265,474 - GFF2840 0.86 +0.3 265,474 - GFF2840 0.86 +1.6 265,474 - GFF2840 0.86 -0.3 265,474 - GFF2840 0.86 +0.7 265,474 - GFF2840 0.86 -0.1 265,474 - GFF2840 0.86 -0.8 265,474 - GFF2840 0.86 -1.0 265,474 - GFF2840 0.86 -0.4 265,474 - GFF2840 0.86 -0.7 265,474 - GFF2840 0.86 -0.4 265,474 - GFF2840 0.86 +0.0 265,497 + GFF2840 0.90 -0.2 265,497 + GFF2840 0.90 +0.3 265,497 + GFF2840 0.90 -2.1 265,497 + GFF2840 0.90 -2.1 265,497 + GFF2840 0.90 -1.1 265,498 - -0.5 265,498 - -1.8 265,498 - -0.6 265,498 - +1.6 265,603 + -1.3 265,603 + -0.7 265,603 + -1.0 265,603 + -0.7 265,603 + -0.1 265,603 + +0.4 265,603 + +0.6 265,603 + -1.1 265,603 + -0.3 265,603 + -0.9 265,603 + +0.1 265,603 + -2.1 265,603 + -0.5 265,604 - +0.5 265,604 - -0.3 265,604 - +0.0 265,604 - -2.3 265,604 - -0.9 265,604 - -1.1 265,604 - +0.1 265,604 - -0.7 265,604 - +1.1 265,604 - -1.4 265,604 - +0.3 265,604 - -2.0 265,604 - -0.9 265,604 - -1.3 265,604 - -0.7 265,604 - +1.6 265,604 - +0.2 265,604 - +0.9
Or see this region's nucleotide sequence