Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF267 and GFF268 are separated by 63 nucleotides GFF268 and GFF269 are separated by 39 nucleotides GFF269 and GFF270 are separated by 18 nucleotides
GFF267 - no description, at 302,110 to 302,352
GFF267
GFF268 - Carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23), at 302,416 to 303,279
GFF268
GFF269 - 2-methylaconitate isomerase, at 303,319 to 304,509
GFF269
GFF270 - 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (EC 4.2.1.117), at 304,528 to 307,146
GFF270
Position (kb) 303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 302.405 kb on + strand at 302.427 kb on - strand at 302.488 kb on + strand at 302.489 kb on - strand at 302.489 kb on - strand at 302.489 kb on - strand at 302.489 kb on - strand at 302.588 kb on + strand, within GFF268 at 302.588 kb on + strand, within GFF268 at 302.589 kb on - strand, within GFF268 at 302.589 kb on - strand, within GFF268 at 302.604 kb on - strand, within GFF268 at 303.161 kb on + strand, within GFF268 at 303.161 kb on + strand, within GFF268 at 303.161 kb on + strand, within GFF268 at 303.161 kb on + strand, within GFF268 at 303.161 kb on + strand, within GFF268 at 303.161 kb on + strand, within GFF268 at 303.161 kb on + strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.162 kb on - strand, within GFF268 at 303.294 kb on + strand at 303.294 kb on + strand at 303.294 kb on + strand at 303.294 kb on + strand at 303.295 kb on - strand at 303.295 kb on - strand at 303.295 kb on - strand at 303.295 kb on - strand at 303.295 kb on - strand at 303.312 kb on + strand at 303.313 kb on - strand at 303.313 kb on - strand at 303.648 kb on - strand, within GFF269 at 303.819 kb on - strand, within GFF269 at 304.253 kb on + strand, within GFF269 at 304.253 kb on + strand, within GFF269 at 304.253 kb on + strand, within GFF269 at 304.253 kb on + strand, within GFF269 at 304.254 kb on - strand, within GFF269 at 304.254 kb on - strand, within GFF269 at 304.254 kb on - strand, within GFF269 at 304.254 kb on - strand, within GFF269 at 304.254 kb on - strand, within GFF269 at 304.254 kb on - strand, within GFF269 at 304.254 kb on - strand, within GFF269 at 304.254 kb on - strand, within GFF269 at 304.349 kb on + strand, within GFF269 at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.472 kb on + strand at 304.473 kb on - strand at 304.473 kb on - strand at 304.473 kb on - strand at 304.473 kb on - strand at 304.473 kb on - strand at 304.473 kb on - strand at 304.473 kb on - strand at 304.616 kb on + strand at 304.616 kb on + strand at 304.616 kb on + strand at 304.616 kb on - strand at 304.617 kb on - strand at 304.617 kb on - strand at 304.617 kb on - strand at 304.739 kb on + strand at 304.739 kb on + strand at 304.739 kb on + strand at 304.740 kb on - strand at 304.740 kb on - strand at 304.740 kb on - strand at 304.740 kb on - strand at 304.922 kb on + strand, within GFF270 at 304.923 kb on - strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.967 kb on + strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 304.968 kb on - strand, within GFF270 at 305.135 kb on + strand, within GFF270 at 305.136 kb on - strand, within GFF270 at 305.171 kb on + strand, within GFF270 at 305.171 kb on + strand, within GFF270 at 305.171 kb on + strand, within GFF270 at 305.171 kb on + strand, within GFF270 at 305.171 kb on + strand, within GFF270 at 305.171 kb on + strand, within GFF270 at 305.171 kb on + strand, within GFF270 at 305.172 kb on - strand, within GFF270 at 305.172 kb on - strand, within GFF270 at 305.172 kb on - strand, within GFF270 at 305.172 kb on - strand, within GFF270 at 305.172 kb on - strand, within GFF270 at 305.178 kb on - strand, within GFF270 at 305.178 kb on - strand, within GFF270 at 305.178 kb on - strand, within GFF270 at 305.178 kb on - strand, within GFF270 at 305.327 kb on + strand, within GFF270 at 305.327 kb on + strand, within GFF270 at 305.327 kb on + strand, within GFF270 at 305.327 kb on + strand, within GFF270 at 305.327 kb on + strand, within GFF270 at 305.327 kb on + strand, within GFF270 at 305.327 kb on + strand, within GFF270 at 305.328 kb on - strand, within GFF270 at 305.328 kb on - strand, within GFF270 at 305.328 kb on - strand, within GFF270 at 305.328 kb on - strand, within GFF270 at 305.328 kb on - strand, within GFF270 at 305.328 kb on - strand, within GFF270 at 305.351 kb on + strand, within GFF270 at 305.351 kb on + strand, within GFF270 at 305.405 kb on + strand, within GFF270 at 305.405 kb on + strand, within GFF270 at 305.405 kb on + strand, within GFF270 at 305.405 kb on + strand, within GFF270 at 305.406 kb on - strand, within GFF270 at 305.406 kb on - strand, within GFF270 at 305.406 kb on - strand, within GFF270 at 305.406 kb on - strand, within GFF270 at 305.406 kb on - strand, within GFF270
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 302,405 + -0.4 302,427 - +0.8 302,488 + +0.3 302,489 - -1.2 302,489 - -0.5 302,489 - -0.7 302,489 - -1.1 302,588 + GFF268 0.20 -1.9 302,588 + GFF268 0.20 -0.0 302,589 - GFF268 0.20 -1.2 302,589 - GFF268 0.20 -1.0 302,604 - GFF268 0.22 -0.2 303,161 + GFF268 0.86 +0.2 303,161 + GFF268 0.86 -1.8 303,161 + GFF268 0.86 +1.7 303,161 + GFF268 0.86 -1.5 303,161 + GFF268 0.86 -0.5 303,161 + GFF268 0.86 +0.3 303,161 + GFF268 0.86 -1.0 303,162 - GFF268 0.86 -1.4 303,162 - GFF268 0.86 -1.1 303,162 - GFF268 0.86 -0.2 303,162 - GFF268 0.86 -0.7 303,162 - GFF268 0.86 -1.2 303,162 - GFF268 0.86 -1.0 303,162 - GFF268 0.86 -0.8 303,162 - GFF268 0.86 -0.3 303,162 - GFF268 0.86 -0.1 303,162 - GFF268 0.86 -1.6 303,294 + -0.3 303,294 + -0.5 303,294 + -0.3 303,294 + -1.6 303,295 - -1.2 303,295 - +0.3 303,295 - -1.1 303,295 - -0.7 303,295 - +0.2 303,312 + +0.5 303,313 - +0.9 303,313 - +0.2 303,648 - GFF269 0.28 -1.3 303,819 - GFF269 0.42 -0.3 304,253 + GFF269 0.78 -0.3 304,253 + GFF269 0.78 -0.7 304,253 + GFF269 0.78 -2.7 304,253 + GFF269 0.78 -0.7 304,254 - GFF269 0.79 -0.1 304,254 - GFF269 0.79 -1.1 304,254 - GFF269 0.79 -0.4 304,254 - GFF269 0.79 +0.3 304,254 - GFF269 0.79 -0.8 304,254 - GFF269 0.79 -2.2 304,254 - GFF269 0.79 +1.3 304,254 - GFF269 0.79 -0.0 304,349 + GFF269 0.86 -0.6 304,472 + -0.5 304,472 + -0.2 304,472 + -2.3 304,472 + -1.5 304,472 + -1.7 304,472 + -1.3 304,472 + -1.0 304,472 + -1.3 304,472 + +0.1 304,472 + -1.5 304,472 + -0.3 304,472 + -1.4 304,473 - -1.3 304,473 - -1.1 304,473 - -1.0 304,473 - -1.0 304,473 - -1.7 304,473 - -1.9 304,473 - -0.3 304,616 + -2.9 304,616 + -2.3 304,616 + -2.2 304,616 - -1.3 304,617 - +0.1 304,617 - -2.4 304,617 - -0.3 304,739 + -1.1 304,739 + -2.0 304,739 + -1.5 304,740 - -0.9 304,740 - -0.7 304,740 - -1.0 304,740 - -2.9 304,922 + GFF270 0.15 -2.3 304,923 - GFF270 0.15 +0.3 304,967 + GFF270 0.17 -0.2 304,967 + GFF270 0.17 -0.9 304,967 + GFF270 0.17 -2.0 304,967 + GFF270 0.17 -0.6 304,967 + GFF270 0.17 -2.5 304,967 + GFF270 0.17 -3.2 304,967 + GFF270 0.17 -0.5 304,967 + GFF270 0.17 -3.4 304,967 + GFF270 0.17 -1.6 304,967 + GFF270 0.17 -1.7 304,967 + GFF270 0.17 -1.0 304,967 + GFF270 0.17 -0.7 304,967 + GFF270 0.17 +0.9 304,967 + GFF270 0.17 -2.4 304,967 + GFF270 0.17 -0.5 304,967 + GFF270 0.17 -1.1 304,967 + GFF270 0.17 +1.7 304,968 - GFF270 0.17 -1.7 304,968 - GFF270 0.17 -0.7 304,968 - GFF270 0.17 +0.4 304,968 - GFF270 0.17 +0.1 304,968 - GFF270 0.17 -0.4 304,968 - GFF270 0.17 -0.3 304,968 - GFF270 0.17 +1.3 304,968 - GFF270 0.17 -1.9 304,968 - GFF270 0.17 -2.0 304,968 - GFF270 0.17 -2.2 304,968 - GFF270 0.17 -3.0 304,968 - GFF270 0.17 -1.9 304,968 - GFF270 0.17 -2.7 304,968 - GFF270 0.17 -1.5 304,968 - GFF270 0.17 -1.3 305,135 + GFF270 0.23 -1.6 305,136 - GFF270 0.23 -1.3 305,171 + GFF270 0.25 +0.7 305,171 + GFF270 0.25 -1.0 305,171 + GFF270 0.25 -1.2 305,171 + GFF270 0.25 -2.2 305,171 + GFF270 0.25 -1.3 305,171 + GFF270 0.25 -1.9 305,171 + GFF270 0.25 -1.5 305,172 - GFF270 0.25 -2.1 305,172 - GFF270 0.25 -0.9 305,172 - GFF270 0.25 -1.0 305,172 - GFF270 0.25 -2.2 305,172 - GFF270 0.25 -1.1 305,178 - GFF270 0.25 -2.1 305,178 - GFF270 0.25 -0.0 305,178 - GFF270 0.25 -1.5 305,178 - GFF270 0.25 -1.7 305,327 + GFF270 0.31 -2.0 305,327 + GFF270 0.31 -0.9 305,327 + GFF270 0.31 -2.5 305,327 + GFF270 0.31 +0.2 305,327 + GFF270 0.31 -2.2 305,327 + GFF270 0.31 -1.8 305,327 + GFF270 0.31 -2.1 305,328 - GFF270 0.31 -1.9 305,328 - GFF270 0.31 -0.2 305,328 - GFF270 0.31 +0.9 305,328 - GFF270 0.31 -0.3 305,328 - GFF270 0.31 -0.5 305,328 - GFF270 0.31 -0.9 305,351 + GFF270 0.31 -1.6 305,351 + GFF270 0.31 -0.9 305,405 + GFF270 0.33 -2.0 305,405 + GFF270 0.33 -1.3 305,405 + GFF270 0.33 -1.3 305,405 + GFF270 0.33 -1.3 305,406 - GFF270 0.34 +0.2 305,406 - GFF270 0.34 +1.5 305,406 - GFF270 0.34 -1.3 305,406 - GFF270 0.34 -0.6 305,406 - GFF270 0.34 -1.8
Or see this region's nucleotide sequence