Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2330 and GFF2331 are separated by 39 nucleotides GFF2331 and GFF2332 are separated by 111 nucleotides GFF2332 and GFF2333 are separated by 32 nucleotides GFF2333 and GFF2334 are separated by 30 nucleotides
GFF2330 - FOG: TPR repeat, at 380,934 to 382,289
GFF2330
GFF2331 - Tautomerase / Tautomerase, at 382,329 to 382,727
GFF2331
GFF2332 - Transcriptional regulator, LysR family, at 382,839 to 383,699
GFF2332
GFF2333 - hypothetical protein, at 383,732 to 384,142
GFF2333
GFF2334 - Beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily, at 384,173 to 385,363
GFF2334
Position (kb) 382
383
384 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 382.004 kb on - strand, within GFF2330 at 382.117 kb on + strand, within GFF2330 at 382.118 kb on - strand, within GFF2330 at 382.381 kb on + strand, within GFF2331 at 382.382 kb on - strand, within GFF2331 at 382.382 kb on - strand, within GFF2331 at 382.414 kb on + strand, within GFF2331 at 382.414 kb on + strand, within GFF2331 at 382.414 kb on + strand, within GFF2331 at 382.415 kb on - strand, within GFF2331 at 382.415 kb on - strand, within GFF2331 at 382.415 kb on - strand, within GFF2331 at 382.415 kb on - strand, within GFF2331 at 382.475 kb on - strand, within GFF2331 at 382.594 kb on + strand, within GFF2331 at 382.594 kb on + strand, within GFF2331 at 382.594 kb on + strand, within GFF2331 at 382.595 kb on - strand, within GFF2331 at 382.741 kb on - strand at 382.802 kb on + strand at 383.140 kb on - strand, within GFF2332 at 383.466 kb on + strand, within GFF2332 at 383.533 kb on + strand, within GFF2332 at 383.533 kb on + strand, within GFF2332 at 383.534 kb on - strand, within GFF2332 at 383.534 kb on - strand, within GFF2332 at 383.760 kb on - strand at 383.760 kb on - strand at 383.768 kb on + strand at 383.768 kb on + strand at 383.768 kb on + strand at 383.769 kb on - strand at 383.769 kb on - strand at 383.864 kb on + strand, within GFF2333 at 383.888 kb on + strand, within GFF2333 at 383.889 kb on - strand, within GFF2333 at 383.889 kb on - strand, within GFF2333 at 383.889 kb on - strand, within GFF2333 at 384.044 kb on + strand, within GFF2333 at 384.291 kb on + strand at 384.291 kb on + strand at 384.292 kb on - strand, within GFF2334 at 384.292 kb on - strand, within GFF2334 at 384.292 kb on - strand, within GFF2334 at 384.292 kb on - strand, within GFF2334 at 384.292 kb on - strand, within GFF2334 at 384.366 kb on + strand, within GFF2334 at 384.367 kb on - strand, within GFF2334 at 384.651 kb on + strand, within GFF2334 at 384.651 kb on + strand, within GFF2334 at 384.652 kb on - strand, within GFF2334 at 384.673 kb on - strand, within GFF2334 at 384.673 kb on - strand, within GFF2334
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 382,004 - GFF2330 0.79 -2.2 382,117 + GFF2330 0.87 +0.6 382,118 - GFF2330 0.87 -0.5 382,381 + GFF2331 0.13 +0.5 382,382 - GFF2331 0.13 -0.5 382,382 - GFF2331 0.13 +0.1 382,414 + GFF2331 0.21 -0.1 382,414 + GFF2331 0.21 +3.1 382,414 + GFF2331 0.21 +0.4 382,415 - GFF2331 0.22 -0.8 382,415 - GFF2331 0.22 +1.2 382,415 - GFF2331 0.22 -0.3 382,415 - GFF2331 0.22 -0.4 382,475 - GFF2331 0.37 -0.8 382,594 + GFF2331 0.66 +0.5 382,594 + GFF2331 0.66 -0.7 382,594 + GFF2331 0.66 -1.9 382,595 - GFF2331 0.67 -1.0 382,741 - +0.1 382,802 + +0.1 383,140 - GFF2332 0.35 +0.1 383,466 + GFF2332 0.73 -0.8 383,533 + GFF2332 0.81 -1.1 383,533 + GFF2332 0.81 -1.2 383,534 - GFF2332 0.81 -1.2 383,534 - GFF2332 0.81 -0.3 383,760 - -1.1 383,760 - -0.9 383,768 + -0.7 383,768 + -0.1 383,768 + -0.0 383,769 - +0.2 383,769 - +0.2 383,864 + GFF2333 0.32 -1.6 383,888 + GFF2333 0.38 -0.6 383,889 - GFF2333 0.38 -0.6 383,889 - GFF2333 0.38 +0.9 383,889 - GFF2333 0.38 +0.2 384,044 + GFF2333 0.76 -0.2 384,291 + +0.5 384,291 + -1.2 384,292 - GFF2334 0.10 -0.6 384,292 - GFF2334 0.10 -0.0 384,292 - GFF2334 0.10 -1.0 384,292 - GFF2334 0.10 -0.8 384,292 - GFF2334 0.10 -0.2 384,366 + GFF2334 0.16 -0.7 384,367 - GFF2334 0.16 +0.8 384,651 + GFF2334 0.40 +0.1 384,651 + GFF2334 0.40 -1.4 384,652 - GFF2334 0.40 -1.2 384,673 - GFF2334 0.42 +0.2 384,673 - GFF2334 0.42 -0.2
Or see this region's nucleotide sequence