Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF225 and GFF226 are separated by 16 nucleotides GFF226 and GFF227 overlap by 1 nucleotides
GFF225 - Permeases of the major facilitator superfamily, at 251,639 to 252,835
GFF225
GFF226 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 252,852 to 254,492
GFF226
GFF227 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 254,492 to 255,532
GFF227
Position (kb)
252
253
254
255 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 251.980 kb on - strand, within GFF225 at 251.980 kb on - strand, within GFF225 at 252.079 kb on - strand, within GFF225 at 252.140 kb on - strand, within GFF225 at 252.140 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.148 kb on - strand, within GFF225 at 252.169 kb on - strand, within GFF225 at 252.411 kb on + strand, within GFF225 at 252.411 kb on + strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.412 kb on - strand, within GFF225 at 252.651 kb on + strand, within GFF225 at 252.651 kb on + strand, within GFF225 at 252.651 kb on + strand, within GFF225 at 252.652 kb on - strand, within GFF225 at 252.652 kb on - strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.660 kb on + strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.661 kb on - strand, within GFF225 at 252.853 kb on + strand at 252.853 kb on + strand at 252.887 kb on + strand at 252.887 kb on + strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.888 kb on - strand at 252.890 kb on - strand at 252.890 kb on - strand at 252.890 kb on - strand at 252.890 kb on - strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.015 kb on + strand at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.016 kb on - strand, within GFF226 at 253.296 kb on + strand, within GFF226 at 253.296 kb on + strand, within GFF226 at 253.297 kb on - strand, within GFF226 at 253.481 kb on - strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.613 kb on - strand, within GFF226 at 253.613 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226 at 253.855 kb on + strand, within GFF226 at 253.855 kb on + strand, within GFF226 at 253.855 kb on + strand, within GFF226 at 253.855 kb on + strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 254.146 kb on + strand, within GFF226 at 254.146 kb on + strand, within GFF226 at 254.147 kb on - strand, within GFF226 at 254.273 kb on - strand, within GFF226 at 254.273 kb on - strand, within GFF226 at 254.273 kb on - strand, within GFF226 at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.611 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.859 kb on + strand, within GFF227 at 254.859 kb on + strand, within GFF227 at 254.860 kb on - strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.976 kb on - strand, within GFF227 at 254.976 kb on - strand, within GFF227 at 254.976 kb on - strand, within GFF227 at 254.976 kb on - strand, within GFF227 at 255.021 kb on + strand, within GFF227 at 255.021 kb on + strand, within GFF227 at 255.022 kb on - strand, within GFF227 at 255.108 kb on + strand, within GFF227 at 255.108 kb on + strand, within GFF227 at 255.109 kb on - strand, within GFF227 at 255.109 kb on - strand, within GFF227 at 255.109 kb on - strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.202 kb on - strand, within GFF227 at 255.202 kb on - strand, within GFF227 at 255.202 kb on - strand, within GFF227 at 255.202 kb on - strand, within GFF227 at 255.223 kb on - strand, within GFF227 at 255.237 kb on + strand, within GFF227 at 255.238 kb on - strand, within GFF227 at 255.238 kb on - strand, within GFF227 at 255.238 kb on - strand, within GFF227 at 255.279 kb on + strand, within GFF227 at 255.279 kb on + strand, within GFF227 at 255.279 kb on + strand, within GFF227 at 255.279 kb on + strand, within GFF227 at 255.280 kb on - strand, within GFF227 at 255.280 kb on - strand, within GFF227 at 255.280 kb on - strand, within GFF227 at 255.291 kb on + strand, within GFF227 at 255.336 kb on + strand, within GFF227 at 255.336 kb on + strand, within GFF227 at 255.336 kb on + strand, within GFF227 at 255.337 kb on - strand, within GFF227 at 255.337 kb on - strand, within GFF227 at 255.337 kb on - strand, within GFF227 at 255.337 kb on - strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.362 kb on - strand, within GFF227 at 255.362 kb on - strand, within GFF227 at 255.362 kb on - strand, within GFF227 at 255.372 kb on + strand, within GFF227 at 255.373 kb on - strand, within GFF227 at 255.373 kb on - strand, within GFF227
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 251,980 - GFF225 0.28 +1.0 251,980 - GFF225 0.28 -1.5 252,079 - GFF225 0.37 +0.2 252,140 - GFF225 0.42 -0.6 252,140 - GFF225 0.42 +1.3 252,148 - GFF225 0.43 -0.5 252,148 - GFF225 0.43 -0.4 252,148 - GFF225 0.43 -0.3 252,148 - GFF225 0.43 -0.2 252,148 - GFF225 0.43 -0.1 252,169 - GFF225 0.44 -0.5 252,411 + GFF225 0.64 -0.5 252,411 + GFF225 0.64 -0.9 252,412 - GFF225 0.65 -0.7 252,412 - GFF225 0.65 -0.3 252,412 - GFF225 0.65 -2.0 252,412 - GFF225 0.65 +0.7 252,412 - GFF225 0.65 -1.6 252,412 - GFF225 0.65 +0.2 252,651 + GFF225 0.85 -0.7 252,651 + GFF225 0.85 +1.2 252,651 + GFF225 0.85 -0.4 252,652 - GFF225 0.85 -0.0 252,652 - GFF225 0.85 -2.6 252,660 + GFF225 0.85 -0.2 252,660 + GFF225 0.85 +0.8 252,660 + GFF225 0.85 -0.4 252,660 + GFF225 0.85 +0.6 252,660 + GFF225 0.85 -1.6 252,661 - GFF225 0.85 -0.2 252,661 - GFF225 0.85 +0.1 252,661 - GFF225 0.85 -0.5 252,661 - GFF225 0.85 +0.2 252,661 - GFF225 0.85 -0.2 252,661 - GFF225 0.85 -0.7 252,661 - GFF225 0.85 -0.4 252,853 + -0.8 252,853 + -1.7 252,887 + -0.3 252,887 + -0.3 252,888 - +0.7 252,888 - -0.0 252,888 - -0.5 252,888 - -1.4 252,888 - +1.1 252,888 - +0.2 252,888 - +0.3 252,888 - -0.1 252,890 - +0.4 252,890 - -0.1 252,890 - -1.2 252,890 - -0.2 253,015 + +0.1 253,015 + -1.5 253,015 + -0.8 253,015 + -1.2 253,015 + -2.6 253,015 + -0.7 253,016 - GFF226 0.10 -0.0 253,016 - GFF226 0.10 -1.3 253,016 - GFF226 0.10 -0.7 253,016 - GFF226 0.10 -1.5 253,016 - GFF226 0.10 -0.9 253,016 - GFF226 0.10 -0.4 253,016 - GFF226 0.10 -0.7 253,016 - GFF226 0.10 +0.5 253,296 + GFF226 0.27 -0.7 253,296 + GFF226 0.27 -0.8 253,297 - GFF226 0.27 -0.5 253,481 - GFF226 0.38 -1.2 253,612 + GFF226 0.46 -1.3 253,612 + GFF226 0.46 -2.6 253,612 + GFF226 0.46 -1.1 253,612 + GFF226 0.46 -0.8 253,612 + GFF226 0.46 -1.3 253,612 + GFF226 0.46 +0.7 253,613 - GFF226 0.46 -1.2 253,613 - GFF226 0.46 -2.3 253,718 - GFF226 0.53 -0.9 253,718 - GFF226 0.53 -1.8 253,718 - GFF226 0.53 -0.1 253,855 + GFF226 0.61 -1.6 253,855 + GFF226 0.61 -0.5 253,855 + GFF226 0.61 -1.0 253,855 + GFF226 0.61 -1.1 253,856 - GFF226 0.61 -0.6 253,856 - GFF226 0.61 -1.4 253,856 - GFF226 0.61 +0.4 253,856 - GFF226 0.61 -0.6 253,856 - GFF226 0.61 +1.3 253,856 - GFF226 0.61 -1.4 253,856 - GFF226 0.61 -1.7 253,856 - GFF226 0.61 +0.1 254,146 + GFF226 0.79 -0.7 254,146 + GFF226 0.79 -0.8 254,147 - GFF226 0.79 -2.2 254,273 - GFF226 0.87 +1.0 254,273 - GFF226 0.87 +0.7 254,273 - GFF226 0.87 +0.1 254,474 + +0.3 254,474 + +0.2 254,474 + -0.1 254,474 + -0.5 254,474 + -0.3 254,474 + +0.2 254,474 + -2.5 254,474 + +1.7 254,475 - -0.4 254,475 - -0.1 254,475 - +0.9 254,475 - -0.3 254,475 - -0.2 254,475 - -0.4 254,475 - -1.0 254,611 + GFF227 0.11 -0.3 254,820 + GFF227 0.32 +0.0 254,820 + GFF227 0.32 -1.5 254,820 + GFF227 0.32 -1.0 254,820 + GFF227 0.32 +0.6 254,820 + GFF227 0.32 -0.5 254,820 + GFF227 0.32 -0.6 254,820 + GFF227 0.32 -0.7 254,820 + GFF227 0.32 -0.7 254,820 + GFF227 0.32 +1.3 254,821 - GFF227 0.32 -0.9 254,821 - GFF227 0.32 -0.6 254,821 - GFF227 0.32 -1.0 254,821 - GFF227 0.32 -1.0 254,821 - GFF227 0.32 -1.1 254,821 - GFF227 0.32 -1.5 254,859 + GFF227 0.35 +0.3 254,859 + GFF227 0.35 -0.6 254,860 - GFF227 0.35 -0.6 254,952 + GFF227 0.44 -1.5 254,952 + GFF227 0.44 -2.2 254,952 + GFF227 0.44 -1.7 254,952 + GFF227 0.44 +0.6 254,952 + GFF227 0.44 +0.9 254,952 + GFF227 0.44 -0.0 254,953 - GFF227 0.44 -1.4 254,953 - GFF227 0.44 -1.0 254,953 - GFF227 0.44 -1.0 254,953 - GFF227 0.44 -0.5 254,953 - GFF227 0.44 -1.2 254,953 - GFF227 0.44 -1.1 254,953 - GFF227 0.44 +0.1 254,975 + GFF227 0.46 -0.1 254,975 + GFF227 0.46 -1.2 254,975 + GFF227 0.46 -1.1 254,975 + GFF227 0.46 -0.7 254,975 + GFF227 0.46 -1.4 254,975 + GFF227 0.46 -0.3 254,975 + GFF227 0.46 +0.1 254,975 + GFF227 0.46 -0.5 254,976 - GFF227 0.46 -0.3 254,976 - GFF227 0.46 -0.9 254,976 - GFF227 0.46 -1.5 254,976 - GFF227 0.46 -0.5 255,021 + GFF227 0.51 -0.3 255,021 + GFF227 0.51 -1.3 255,022 - GFF227 0.51 -0.5 255,108 + GFF227 0.59 -0.3 255,108 + GFF227 0.59 -1.5 255,109 - GFF227 0.59 -0.9 255,109 - GFF227 0.59 -1.7 255,109 - GFF227 0.59 -0.3 255,201 + GFF227 0.68 -0.7 255,201 + GFF227 0.68 +0.3 255,201 + GFF227 0.68 -0.7 255,201 + GFF227 0.68 +1.0 255,201 + GFF227 0.68 -0.5 255,201 + GFF227 0.68 -1.4 255,201 + GFF227 0.68 -0.7 255,201 + GFF227 0.68 -0.0 255,202 - GFF227 0.68 +0.6 255,202 - GFF227 0.68 -0.7 255,202 - GFF227 0.68 -1.0 255,202 - GFF227 0.68 +0.0 255,223 - GFF227 0.70 -1.5 255,237 + GFF227 0.72 +0.1 255,238 - GFF227 0.72 -2.5 255,238 - GFF227 0.72 +1.3 255,238 - GFF227 0.72 -0.4 255,279 + GFF227 0.76 +0.3 255,279 + GFF227 0.76 +0.3 255,279 + GFF227 0.76 +1.9 255,279 + GFF227 0.76 -0.6 255,280 - GFF227 0.76 -0.4 255,280 - GFF227 0.76 -1.4 255,280 - GFF227 0.76 -0.3 255,291 + GFF227 0.77 -0.9 255,336 + GFF227 0.81 -0.1 255,336 + GFF227 0.81 -1.5 255,336 + GFF227 0.81 -0.7 255,337 - GFF227 0.81 -1.0 255,337 - GFF227 0.81 -1.1 255,337 - GFF227 0.81 +0.2 255,337 - GFF227 0.81 -0.4 255,357 + GFF227 0.83 -0.4 255,357 + GFF227 0.83 -1.5 255,357 + GFF227 0.83 -0.4 255,357 + GFF227 0.83 -0.7 255,357 + GFF227 0.83 -0.8 255,358 - GFF227 0.83 -0.1 255,358 - GFF227 0.83 -0.8 255,358 - GFF227 0.83 +0.3 255,358 - GFF227 0.83 -1.0 255,358 - GFF227 0.83 -1.3 255,358 - GFF227 0.83 -0.9 255,358 - GFF227 0.83 -1.9 255,358 - GFF227 0.83 -1.5 255,361 + GFF227 0.83 +0.3 255,361 + GFF227 0.83 -0.2 255,361 + GFF227 0.83 +0.4 255,361 + GFF227 0.83 -1.2 255,361 + GFF227 0.83 +0.6 255,362 - GFF227 0.84 -1.7 255,362 - GFF227 0.84 -1.6 255,362 - GFF227 0.84 -1.6 255,372 + GFF227 0.85 -0.7 255,373 - GFF227 0.85 -0.3 255,373 - GFF227 0.85 -0.8
Or see this region's nucleotide sequence