Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2248 and GFF2249 are separated by 47 nucleotides GFF2249 and GFF2250 are separated by 512 nucleotides GFF2250 and GFF2251 are separated by 55 nucleotides GFF2251 and GFF2252 are separated by 16 nucleotides
GFF2248 - Transcriptional regulatory protein RtcR, at 304,186 to 305,808
GFF2248
GFF2249 - no description, at 305,856 to 305,990
GFF2249
GFF2250 - Putative stomatin/prohibitin-family membrane protease subunit aq_911, at 306,503 to 307,654
GFF2250
GFF2251 - no description, at 307,710 to 308,204
GFF2251
GFF2252 - RNA-2',3'-PO4:RNA-5'-OH ligase, at 308,221 to 309,456
GFF2252
Position (kb) 306
307
308 Strain fitness (log2 ratio) -2
-1
0
1 at 305.516 kb on + strand, within GFF2248 at 305.534 kb on + strand, within GFF2248 at 305.534 kb on + strand, within GFF2248 at 305.534 kb on + strand, within GFF2248 at 305.546 kb on + strand, within GFF2248 at 305.669 kb on + strand at 305.670 kb on - strand at 305.670 kb on - strand at 305.779 kb on + strand at 305.779 kb on + strand at 305.788 kb on + strand at 305.788 kb on + strand at 305.850 kb on - strand at 305.850 kb on - strand at 305.857 kb on + strand at 305.857 kb on + strand at 305.857 kb on + strand at 305.857 kb on + strand at 305.857 kb on + strand at 305.858 kb on - strand at 305.858 kb on - strand at 305.858 kb on - strand at 305.858 kb on - strand at 305.920 kb on + strand, within GFF2249 at 305.921 kb on - strand, within GFF2249 at 306.052 kb on + strand at 306.053 kb on - strand at 306.053 kb on - strand at 306.579 kb on + strand at 306.579 kb on + strand at 306.579 kb on + strand at 306.579 kb on + strand at 306.580 kb on - strand at 306.630 kb on + strand, within GFF2250 at 306.630 kb on + strand, within GFF2250 at 306.680 kb on + strand, within GFF2250 at 306.680 kb on + strand, within GFF2250 at 306.680 kb on + strand, within GFF2250 at 306.680 kb on + strand, within GFF2250 at 306.723 kb on + strand, within GFF2250 at 306.812 kb on + strand, within GFF2250 at 306.813 kb on - strand, within GFF2250 at 307.220 kb on + strand, within GFF2250 at 307.749 kb on + strand at 307.824 kb on + strand, within GFF2251 at 307.962 kb on + strand, within GFF2251 at 308.051 kb on + strand, within GFF2251 at 308.160 kb on + strand at 308.160 kb on + strand at 308.184 kb on + strand at 308.184 kb on + strand at 308.185 kb on - strand at 308.185 kb on - strand at 308.185 kb on - strand at 308.245 kb on + strand at 308.245 kb on + strand at 308.245 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 305,516 + GFF2248 0.82 -1.2 305,534 + GFF2248 0.83 +0.1 305,534 + GFF2248 0.83 -0.4 305,534 + GFF2248 0.83 -0.2 305,546 + GFF2248 0.84 -0.3 305,669 + +0.2 305,670 - -0.5 305,670 - -0.6 305,779 + +0.8 305,779 + +1.1 305,788 + +0.1 305,788 + -0.6 305,850 - +1.3 305,850 - -0.7 305,857 + -0.1 305,857 + -0.8 305,857 + +0.3 305,857 + +0.8 305,857 + -0.3 305,858 - -0.5 305,858 - +0.3 305,858 - -0.0 305,858 - -0.5 305,920 + GFF2249 0.47 -0.2 305,921 - GFF2249 0.48 +0.6 306,052 + -0.4 306,053 - -1.2 306,053 - -0.6 306,579 + -1.1 306,579 + -1.2 306,579 + +0.8 306,579 + +0.2 306,580 - -0.0 306,630 + GFF2250 0.11 +1.1 306,630 + GFF2250 0.11 -0.1 306,680 + GFF2250 0.15 +0.7 306,680 + GFF2250 0.15 +1.7 306,680 + GFF2250 0.15 +0.5 306,680 + GFF2250 0.15 +1.4 306,723 + GFF2250 0.19 -1.0 306,812 + GFF2250 0.27 +1.1 306,813 - GFF2250 0.27 +0.8 307,220 + GFF2250 0.62 +0.3 307,749 + -1.4 307,824 + GFF2251 0.23 -2.1 307,962 + GFF2251 0.51 -2.0 308,051 + GFF2251 0.69 -0.0 308,160 + +1.1 308,160 + -0.6 308,184 + -0.1 308,184 + -0.5 308,185 - +0.4 308,185 - -1.9 308,185 - -0.7 308,245 + -0.6 308,245 + -0.4 308,245 + -0.3
Or see this region's nucleotide sequence