Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2140 and GFF2141 are separated by 208 nucleotides GFF2141 and GFF2142 are separated by 172 nucleotides GFF2142 and GFF2143 are separated by 21 nucleotides
GFF2140 - Ribose ABC transporter, substrate-binding protein RbsB (TC 3.A.1.2.1), at 189,387 to 190,340
GFF2140
GFF2141 - GTP-binding and nucleic acid-binding protein YchF, at 190,549 to 191,640
GFF2141
GFF2142 - Glutathione S-transferase (EC 2.5.1.18), at 191,813 to 192,487
GFF2142
GFF2143 - probable membrane protein YPO3302, at 192,509 to 192,973
GFF2143
Position (kb)
190
191
192 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 189.580 kb on + strand, within GFF2140 at 189.713 kb on + strand, within GFF2140 at 189.713 kb on + strand, within GFF2140 at 189.714 kb on - strand, within GFF2140 at 189.791 kb on - strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.153 kb on + strand, within GFF2140 at 190.154 kb on - strand, within GFF2140 at 190.154 kb on - strand, within GFF2140 at 190.322 kb on + strand at 190.402 kb on + strand at 190.403 kb on - strand at 190.405 kb on + strand at 190.406 kb on - strand at 190.447 kb on + strand at 190.448 kb on - strand at 190.590 kb on + strand at 190.591 kb on - strand at 190.591 kb on - strand at 190.604 kb on + strand at 191.200 kb on + strand, within GFF2141 at 191.200 kb on + strand, within GFF2141 at 191.221 kb on + strand, within GFF2141 at 191.221 kb on + strand, within GFF2141 at 191.221 kb on + strand, within GFF2141 at 191.392 kb on + strand, within GFF2141 at 191.392 kb on + strand, within GFF2141 at 191.392 kb on + strand, within GFF2141 at 191.392 kb on + strand, within GFF2141 at 191.392 kb on + strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.393 kb on - strand, within GFF2141 at 191.494 kb on + strand, within GFF2141 at 191.494 kb on + strand, within GFF2141 at 191.494 kb on + strand, within GFF2141 at 191.494 kb on + strand, within GFF2141 at 191.494 kb on + strand, within GFF2141 at 191.495 kb on - strand, within GFF2141 at 191.495 kb on - strand, within GFF2141 at 191.495 kb on - strand, within GFF2141 at 191.495 kb on - strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.527 kb on + strand, within GFF2141 at 191.528 kb on - strand, within GFF2141 at 191.528 kb on - strand, within GFF2141 at 191.528 kb on - strand, within GFF2141 at 191.528 kb on - strand, within GFF2141 at 191.528 kb on - strand, within GFF2141 at 191.528 kb on - strand, within GFF2141 at 191.528 kb on - strand, within GFF2141 at 191.528 kb on - strand, within GFF2141 at 191.593 kb on + strand at 191.593 kb on + strand at 191.593 kb on + strand at 191.593 kb on + strand at 191.593 kb on + strand at 191.593 kb on + strand at 191.593 kb on + strand at 191.593 kb on + strand at 191.593 kb on + strand at 191.594 kb on - strand at 191.594 kb on - strand at 191.594 kb on - strand at 191.594 kb on - strand at 191.594 kb on - strand at 191.594 kb on - strand at 191.594 kb on - strand at 191.594 kb on - strand at 191.638 kb on + strand at 191.638 kb on + strand at 191.638 kb on + strand at 191.638 kb on + strand at 191.638 kb on + strand at 191.639 kb on - strand at 191.639 kb on - strand at 191.641 kb on + strand at 191.641 kb on + strand at 191.775 kb on + strand at 191.775 kb on + strand at 191.776 kb on - strand at 192.410 kb on + strand, within GFF2142 at 192.411 kb on - strand, within GFF2142 at 192.440 kb on + strand at 192.441 kb on - strand at 192.441 kb on - strand at 192.441 kb on - strand at 192.441 kb on - strand at 192.546 kb on + strand at 192.546 kb on + strand at 192.547 kb on - strand at 192.573 kb on + strand, within GFF2143 at 192.573 kb on + strand, within GFF2143 at 192.573 kb on + strand, within GFF2143 at 192.573 kb on + strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143 at 192.574 kb on - strand, within GFF2143
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 189,580 + GFF2140 0.20 +0.3 189,713 + GFF2140 0.34 -0.3 189,713 + GFF2140 0.34 +0.8 189,714 - GFF2140 0.34 -0.5 189,791 - GFF2140 0.42 +0.7 190,153 + GFF2140 0.80 -1.1 190,153 + GFF2140 0.80 -0.3 190,153 + GFF2140 0.80 -0.6 190,154 - GFF2140 0.80 -0.6 190,154 - GFF2140 0.80 -1.5 190,322 + +0.3 190,402 + -0.6 190,403 - -0.4 190,405 + +0.8 190,406 - -0.2 190,447 + -0.8 190,448 - -0.4 190,590 + -0.1 190,591 - -0.4 190,591 - -0.3 190,604 + -0.9 191,200 + GFF2141 0.60 -0.4 191,200 + GFF2141 0.60 -1.4 191,221 + GFF2141 0.62 -0.6 191,221 + GFF2141 0.62 +0.7 191,221 + GFF2141 0.62 -1.0 191,392 + GFF2141 0.77 -0.2 191,392 + GFF2141 0.77 +0.8 191,392 + GFF2141 0.77 +0.3 191,392 + GFF2141 0.77 +0.8 191,392 + GFF2141 0.77 -0.7 191,393 - GFF2141 0.77 +0.8 191,393 - GFF2141 0.77 +1.4 191,393 - GFF2141 0.77 -1.0 191,393 - GFF2141 0.77 +0.1 191,393 - GFF2141 0.77 -0.8 191,393 - GFF2141 0.77 +0.7 191,393 - GFF2141 0.77 -2.1 191,393 - GFF2141 0.77 -0.9 191,393 - GFF2141 0.77 -1.4 191,494 + GFF2141 0.87 +0.7 191,494 + GFF2141 0.87 +0.4 191,494 + GFF2141 0.87 -0.3 191,494 + GFF2141 0.87 -0.6 191,494 + GFF2141 0.87 -0.3 191,495 - GFF2141 0.87 -0.9 191,495 - GFF2141 0.87 -0.0 191,495 - GFF2141 0.87 +0.2 191,495 - GFF2141 0.87 -0.0 191,527 + GFF2141 0.90 -1.6 191,527 + GFF2141 0.90 -0.6 191,527 + GFF2141 0.90 -0.4 191,527 + GFF2141 0.90 +0.1 191,527 + GFF2141 0.90 -0.2 191,527 + GFF2141 0.90 +0.8 191,527 + GFF2141 0.90 -0.5 191,527 + GFF2141 0.90 -0.2 191,527 + GFF2141 0.90 -0.4 191,528 - GFF2141 0.90 +0.8 191,528 - GFF2141 0.90 +0.4 191,528 - GFF2141 0.90 +0.7 191,528 - GFF2141 0.90 -1.2 191,528 - GFF2141 0.90 -0.2 191,528 - GFF2141 0.90 -0.8 191,528 - GFF2141 0.90 -0.2 191,528 - GFF2141 0.90 -0.3 191,593 + +0.2 191,593 + +3.4 191,593 + -0.2 191,593 + +0.2 191,593 + -0.6 191,593 + +0.5 191,593 + +1.0 191,593 + -2.0 191,593 + -0.5 191,594 - -1.2 191,594 - +0.8 191,594 - -0.3 191,594 - -0.7 191,594 - -0.5 191,594 - -1.1 191,594 - -0.3 191,594 - -2.7 191,638 + -0.1 191,638 + -0.6 191,638 + -0.4 191,638 + -0.5 191,638 + -0.6 191,639 - -0.8 191,639 - +0.3 191,641 + +0.5 191,641 + -1.1 191,775 + -1.5 191,775 + -0.9 191,776 - +0.2 192,410 + GFF2142 0.88 -0.2 192,411 - GFF2142 0.89 +1.2 192,440 + -0.1 192,441 - -0.7 192,441 - +0.2 192,441 - -0.6 192,441 - -1.2 192,546 + -0.2 192,546 + -0.1 192,547 - -0.7 192,573 + GFF2143 0.14 +1.6 192,573 + GFF2143 0.14 -0.8 192,573 + GFF2143 0.14 -0.7 192,573 + GFF2143 0.14 +0.8 192,574 - GFF2143 0.14 +0.6 192,574 - GFF2143 0.14 -0.1 192,574 - GFF2143 0.14 +1.5 192,574 - GFF2143 0.14 -0.4 192,574 - GFF2143 0.14 -2.1 192,574 - GFF2143 0.14 -0.5 192,574 - GFF2143 0.14 -1.0 192,574 - GFF2143 0.14 -1.2 192,574 - GFF2143 0.14 +0.0
Or see this region's nucleotide sequence