Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2031 and GFF2032 are separated by 186 nucleotides GFF2032 and GFF2033 are separated by 7 nucleotides
GFF2031 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 73,765 to 74,877
GFF2031
GFF2032 - Excinuclease ABC subunit A, dimeric form, at 75,064 to 80,907
GFF2032
GFF2033 - no description, at 80,915 to 81,871
GFF2033
Position (kb)
75
76
77
78
79
80
81 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 74.270 kb on + strand, within GFF2031 at 74.270 kb on + strand, within GFF2031 at 74.270 kb on + strand, within GFF2031 at 74.270 kb on + strand, within GFF2031 at 74.271 kb on - strand, within GFF2031 at 74.381 kb on + strand, within GFF2031 at 74.381 kb on + strand, within GFF2031 at 74.381 kb on + strand, within GFF2031 at 74.381 kb on + strand, within GFF2031 at 74.382 kb on - strand, within GFF2031 at 74.382 kb on - strand, within GFF2031 at 74.382 kb on - strand, within GFF2031 at 74.382 kb on - strand, within GFF2031 at 74.382 kb on - strand, within GFF2031 at 74.382 kb on - strand, within GFF2031 at 74.382 kb on - strand, within GFF2031 at 74.382 kb on - strand, within GFF2031 at 74.450 kb on + strand, within GFF2031 at 74.537 kb on + strand, within GFF2031 at 74.537 kb on + strand, within GFF2031 at 74.538 kb on - strand, within GFF2031 at 74.538 kb on - strand, within GFF2031 at 74.538 kb on - strand, within GFF2031 at 74.538 kb on - strand, within GFF2031 at 74.935 kb on + strand at 74.935 kb on + strand at 74.936 kb on - strand at 75.004 kb on - strand at 75.004 kb on - strand at 75.004 kb on - strand at 75.034 kb on - strand at 75.205 kb on + strand at 75.205 kb on + strand at 75.205 kb on + strand at 75.205 kb on + strand at 75.226 kb on + strand at 75.227 kb on - strand at 75.247 kb on + strand at 75.247 kb on + strand at 75.475 kb on + strand at 75.476 kb on - strand at 75.476 kb on - strand at 75.476 kb on - strand at 76.129 kb on + strand, within GFF2032 at 76.129 kb on + strand, within GFF2032 at 76.129 kb on + strand, within GFF2032 at 76.129 kb on + strand, within GFF2032 at 76.130 kb on - strand, within GFF2032 at 76.130 kb on - strand, within GFF2032 at 76.201 kb on + strand, within GFF2032 at 76.201 kb on + strand, within GFF2032 at 76.201 kb on + strand, within GFF2032 at 76.201 kb on + strand, within GFF2032 at 76.234 kb on + strand, within GFF2032 at 76.234 kb on + strand, within GFF2032 at 76.235 kb on - strand, within GFF2032 at 76.243 kb on + strand, within GFF2032 at 76.243 kb on + strand, within GFF2032 at 76.243 kb on + strand, within GFF2032 at 76.243 kb on + strand, within GFF2032 at 76.244 kb on - strand, within GFF2032 at 76.645 kb on + strand, within GFF2032 at 76.646 kb on - strand, within GFF2032 at 76.646 kb on - strand, within GFF2032 at 76.658 kb on + strand, within GFF2032 at 76.658 kb on + strand, within GFF2032 at 76.658 kb on + strand, within GFF2032 at 76.658 kb on + strand, within GFF2032 at 76.658 kb on + strand, within GFF2032 at 76.659 kb on - strand, within GFF2032 at 76.659 kb on - strand, within GFF2032 at 77.008 kb on + strand, within GFF2032 at 77.008 kb on + strand, within GFF2032 at 77.008 kb on + strand, within GFF2032 at 77.009 kb on - strand, within GFF2032 at 77.009 kb on - strand, within GFF2032 at 77.186 kb on + strand, within GFF2032 at 77.186 kb on + strand, within GFF2032 at 77.186 kb on + strand, within GFF2032 at 77.187 kb on - strand, within GFF2032 at 77.187 kb on - strand, within GFF2032 at 77.344 kb on + strand, within GFF2032 at 77.344 kb on + strand, within GFF2032 at 77.345 kb on - strand, within GFF2032 at 77.512 kb on + strand, within GFF2032 at 77.512 kb on + strand, within GFF2032 at 77.512 kb on + strand, within GFF2032 at 77.513 kb on - strand, within GFF2032 at 77.513 kb on - strand, within GFF2032 at 77.513 kb on - strand, within GFF2032 at 77.513 kb on - strand, within GFF2032 at 77.513 kb on - strand, within GFF2032 at 77.593 kb on + strand, within GFF2032 at 77.593 kb on + strand, within GFF2032 at 77.593 kb on + strand, within GFF2032 at 77.593 kb on + strand, within GFF2032 at 77.593 kb on + strand, within GFF2032 at 77.593 kb on + strand, within GFF2032 at 77.594 kb on - strand, within GFF2032 at 77.594 kb on - strand, within GFF2032 at 77.594 kb on - strand, within GFF2032 at 77.594 kb on - strand, within GFF2032 at 77.594 kb on - strand, within GFF2032 at 77.704 kb on + strand, within GFF2032 at 77.704 kb on + strand, within GFF2032 at 77.705 kb on - strand, within GFF2032 at 77.705 kb on - strand, within GFF2032 at 77.705 kb on - strand, within GFF2032 at 77.705 kb on - strand, within GFF2032 at 77.705 kb on - strand, within GFF2032 at 77.705 kb on - strand, within GFF2032 at 78.094 kb on + strand, within GFF2032 at 78.095 kb on - strand, within GFF2032 at 78.095 kb on - strand, within GFF2032 at 78.121 kb on + strand, within GFF2032 at 78.121 kb on + strand, within GFF2032 at 78.122 kb on - strand, within GFF2032 at 78.151 kb on + strand, within GFF2032 at 78.152 kb on - strand, within GFF2032 at 78.152 kb on - strand, within GFF2032 at 78.152 kb on - strand, within GFF2032 at 78.152 kb on - strand, within GFF2032 at 78.337 kb on + strand, within GFF2032 at 78.338 kb on - strand, within GFF2032 at 78.358 kb on + strand, within GFF2032 at 78.358 kb on + strand, within GFF2032 at 78.359 kb on - strand, within GFF2032 at 78.409 kb on + strand, within GFF2032 at 78.409 kb on + strand, within GFF2032 at 78.409 kb on + strand, within GFF2032 at 78.409 kb on + strand, within GFF2032 at 78.409 kb on + strand, within GFF2032 at 78.514 kb on + strand, within GFF2032 at 78.514 kb on + strand, within GFF2032 at 78.514 kb on + strand, within GFF2032 at 78.515 kb on - strand, within GFF2032 at 78.515 kb on - strand, within GFF2032 at 78.515 kb on - strand, within GFF2032 at 78.515 kb on - strand, within GFF2032 at 78.515 kb on - strand, within GFF2032 at 78.664 kb on + strand, within GFF2032 at 78.664 kb on + strand, within GFF2032 at 78.664 kb on + strand, within GFF2032 at 78.664 kb on + strand, within GFF2032 at 78.665 kb on - strand, within GFF2032 at 78.665 kb on - strand, within GFF2032 at 78.665 kb on - strand, within GFF2032 at 78.665 kb on - strand, within GFF2032 at 78.665 kb on - strand, within GFF2032 at 78.998 kb on - strand, within GFF2032 at 78.998 kb on - strand, within GFF2032 at 79.027 kb on + strand, within GFF2032 at 79.027 kb on + strand, within GFF2032 at 79.028 kb on - strand, within GFF2032 at 79.028 kb on - strand, within GFF2032 at 79.426 kb on + strand, within GFF2032 at 79.427 kb on - strand, within GFF2032 at 79.427 kb on - strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.525 kb on + strand, within GFF2032 at 79.526 kb on - strand, within GFF2032 at 79.526 kb on - strand, within GFF2032 at 79.526 kb on - strand, within GFF2032 at 79.526 kb on - strand, within GFF2032 at 79.526 kb on - strand, within GFF2032 at 79.526 kb on - strand, within GFF2032 at 79.769 kb on - strand, within GFF2032 at 79.769 kb on - strand, within GFF2032 at 80.089 kb on + strand, within GFF2032 at 80.090 kb on - strand, within GFF2032 at 80.090 kb on - strand, within GFF2032 at 80.108 kb on + strand, within GFF2032 at 80.164 kb on + strand, within GFF2032 at 80.164 kb on + strand, within GFF2032 at 80.164 kb on + strand, within GFF2032 at 80.164 kb on + strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.512 kb on + strand at 80.513 kb on - strand at 80.513 kb on - strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.642 kb on - strand at 80.642 kb on - strand at 80.642 kb on - strand at 81.119 kb on + strand, within GFF2033 at 81.120 kb on - strand, within GFF2033 at 81.323 kb on + strand, within GFF2033 at 81.323 kb on + strand, within GFF2033 at 81.323 kb on + strand, within GFF2033 at 81.323 kb on + strand, within GFF2033 at 81.324 kb on - strand, within GFF2033 at 81.324 kb on - strand, within GFF2033 at 81.324 kb on - strand, within GFF2033 at 81.837 kb on + strand at 81.837 kb on + strand at 81.837 kb on + strand at 81.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 74,270 + GFF2031 0.45 +0.4 74,270 + GFF2031 0.45 -0.0 74,270 + GFF2031 0.45 -0.5 74,270 + GFF2031 0.45 -0.8 74,271 - GFF2031 0.45 +0.7 74,381 + GFF2031 0.55 -0.4 74,381 + GFF2031 0.55 -0.2 74,381 + GFF2031 0.55 -1.8 74,381 + GFF2031 0.55 +0.6 74,382 - GFF2031 0.55 -0.4 74,382 - GFF2031 0.55 +0.4 74,382 - GFF2031 0.55 -0.9 74,382 - GFF2031 0.55 -0.1 74,382 - GFF2031 0.55 -0.4 74,382 - GFF2031 0.55 +0.5 74,382 - GFF2031 0.55 +1.6 74,382 - GFF2031 0.55 -0.5 74,450 + GFF2031 0.62 +0.0 74,537 + GFF2031 0.69 +0.6 74,537 + GFF2031 0.69 -2.2 74,538 - GFF2031 0.69 +0.6 74,538 - GFF2031 0.69 +0.4 74,538 - GFF2031 0.69 -0.7 74,538 - GFF2031 0.69 +2.1 74,935 + +0.2 74,935 + -1.0 74,936 - -0.8 75,004 - -0.0 75,004 - -0.5 75,004 - -1.2 75,034 - +0.7 75,205 + +0.5 75,205 + -1.5 75,205 + +0.8 75,205 + +0.1 75,226 + +1.2 75,227 - -0.8 75,247 + -1.6 75,247 + -1.1 75,475 + -0.1 75,476 - -0.2 75,476 - +1.0 75,476 - -0.1 76,129 + GFF2032 0.18 -0.4 76,129 + GFF2032 0.18 +0.8 76,129 + GFF2032 0.18 -1.4 76,129 + GFF2032 0.18 -0.6 76,130 - GFF2032 0.18 +0.9 76,130 - GFF2032 0.18 -0.8 76,201 + GFF2032 0.19 -1.3 76,201 + GFF2032 0.19 -1.4 76,201 + GFF2032 0.19 -0.2 76,201 + GFF2032 0.19 +0.2 76,234 + GFF2032 0.20 -0.6 76,234 + GFF2032 0.20 -0.3 76,235 - GFF2032 0.20 -1.6 76,243 + GFF2032 0.20 -0.7 76,243 + GFF2032 0.20 +0.8 76,243 + GFF2032 0.20 +0.6 76,243 + GFF2032 0.20 +1.4 76,244 - GFF2032 0.20 +1.0 76,645 + GFF2032 0.27 -2.0 76,646 - GFF2032 0.27 -0.2 76,646 - GFF2032 0.27 -0.4 76,658 + GFF2032 0.27 +0.1 76,658 + GFF2032 0.27 -1.5 76,658 + GFF2032 0.27 -1.5 76,658 + GFF2032 0.27 -0.4 76,658 + GFF2032 0.27 -0.6 76,659 - GFF2032 0.27 -0.6 76,659 - GFF2032 0.27 -0.2 77,008 + GFF2032 0.33 -1.5 77,008 + GFF2032 0.33 -0.6 77,008 + GFF2032 0.33 +0.8 77,009 - GFF2032 0.33 -0.7 77,009 - GFF2032 0.33 -2.1 77,186 + GFF2032 0.36 -0.4 77,186 + GFF2032 0.36 -1.0 77,186 + GFF2032 0.36 -0.6 77,187 - GFF2032 0.36 -0.3 77,187 - GFF2032 0.36 +0.0 77,344 + GFF2032 0.39 -1.4 77,344 + GFF2032 0.39 +0.5 77,345 - GFF2032 0.39 +2.1 77,512 + GFF2032 0.42 -0.5 77,512 + GFF2032 0.42 -0.1 77,512 + GFF2032 0.42 -0.1 77,513 - GFF2032 0.42 -0.9 77,513 - GFF2032 0.42 -0.8 77,513 - GFF2032 0.42 -0.3 77,513 - GFF2032 0.42 -0.2 77,513 - GFF2032 0.42 -0.7 77,593 + GFF2032 0.43 -0.6 77,593 + GFF2032 0.43 +0.3 77,593 + GFF2032 0.43 -1.2 77,593 + GFF2032 0.43 +0.2 77,593 + GFF2032 0.43 +1.2 77,593 + GFF2032 0.43 -0.3 77,594 - GFF2032 0.43 +0.3 77,594 - GFF2032 0.43 -0.3 77,594 - GFF2032 0.43 -0.2 77,594 - GFF2032 0.43 +0.5 77,594 - GFF2032 0.43 -0.3 77,704 + GFF2032 0.45 -1.2 77,704 + GFF2032 0.45 -0.3 77,705 - GFF2032 0.45 -0.2 77,705 - GFF2032 0.45 -0.2 77,705 - GFF2032 0.45 -0.5 77,705 - GFF2032 0.45 +0.3 77,705 - GFF2032 0.45 +1.0 77,705 - GFF2032 0.45 +0.2 78,094 + GFF2032 0.52 -1.0 78,095 - GFF2032 0.52 -0.9 78,095 - GFF2032 0.52 -0.2 78,121 + GFF2032 0.52 -1.0 78,121 + GFF2032 0.52 -0.2 78,122 - GFF2032 0.52 -1.1 78,151 + GFF2032 0.53 +0.0 78,152 - GFF2032 0.53 +1.8 78,152 - GFF2032 0.53 -0.2 78,152 - GFF2032 0.53 -0.1 78,152 - GFF2032 0.53 +1.3 78,337 + GFF2032 0.56 +0.1 78,338 - GFF2032 0.56 +0.3 78,358 + GFF2032 0.56 +0.0 78,358 + GFF2032 0.56 -0.0 78,359 - GFF2032 0.56 +0.6 78,409 + GFF2032 0.57 -0.1 78,409 + GFF2032 0.57 -0.5 78,409 + GFF2032 0.57 -1.1 78,409 + GFF2032 0.57 -0.5 78,409 + GFF2032 0.57 -0.0 78,514 + GFF2032 0.59 -0.2 78,514 + GFF2032 0.59 -0.5 78,514 + GFF2032 0.59 -0.3 78,515 - GFF2032 0.59 -0.4 78,515 - GFF2032 0.59 +0.1 78,515 - GFF2032 0.59 -0.5 78,515 - GFF2032 0.59 -0.4 78,515 - GFF2032 0.59 -0.1 78,664 + GFF2032 0.62 -1.0 78,664 + GFF2032 0.62 +0.8 78,664 + GFF2032 0.62 -1.1 78,664 + GFF2032 0.62 -1.0 78,665 - GFF2032 0.62 +0.1 78,665 - GFF2032 0.62 +1.2 78,665 - GFF2032 0.62 -0.1 78,665 - GFF2032 0.62 +0.8 78,665 - GFF2032 0.62 -0.5 78,998 - GFF2032 0.67 +0.4 78,998 - GFF2032 0.67 +0.3 79,027 + GFF2032 0.68 -1.0 79,027 + GFF2032 0.68 +0.2 79,028 - GFF2032 0.68 -1.1 79,028 - GFF2032 0.68 +0.1 79,426 + GFF2032 0.75 +0.1 79,427 - GFF2032 0.75 -0.6 79,427 - GFF2032 0.75 -1.0 79,525 + GFF2032 0.76 +0.6 79,525 + GFF2032 0.76 -1.5 79,525 + GFF2032 0.76 -0.1 79,525 + GFF2032 0.76 +0.0 79,525 + GFF2032 0.76 -0.6 79,525 + GFF2032 0.76 -1.7 79,525 + GFF2032 0.76 +0.2 79,525 + GFF2032 0.76 +1.0 79,525 + GFF2032 0.76 +1.3 79,525 + GFF2032 0.76 -0.5 79,526 - GFF2032 0.76 -1.8 79,526 - GFF2032 0.76 -0.5 79,526 - GFF2032 0.76 +0.9 79,526 - GFF2032 0.76 -0.5 79,526 - GFF2032 0.76 +0.2 79,526 - GFF2032 0.76 -0.8 79,769 - GFF2032 0.81 -1.6 79,769 - GFF2032 0.81 -0.5 80,089 + GFF2032 0.86 -0.5 80,090 - GFF2032 0.86 +1.2 80,090 - GFF2032 0.86 -1.2 80,108 + GFF2032 0.86 -0.2 80,164 + GFF2032 0.87 -0.3 80,164 + GFF2032 0.87 +0.8 80,164 + GFF2032 0.87 -0.3 80,164 + GFF2032 0.87 -0.8 80,165 - GFF2032 0.87 -0.4 80,165 - GFF2032 0.87 +1.1 80,165 - GFF2032 0.87 -1.0 80,165 - GFF2032 0.87 -0.5 80,165 - GFF2032 0.87 -0.3 80,165 - GFF2032 0.87 -0.4 80,165 - GFF2032 0.87 -0.7 80,165 - GFF2032 0.87 +0.8 80,165 - GFF2032 0.87 -1.9 80,165 - GFF2032 0.87 -0.9 80,512 + -1.7 80,513 - +0.3 80,513 - -0.1 80,641 + -0.1 80,641 + -0.9 80,641 + +0.2 80,641 + -0.6 80,641 + -0.6 80,641 + -0.8 80,641 + -0.0 80,641 + -0.8 80,641 + -0.4 80,642 - -1.0 80,642 - -0.4 80,642 - +0.7 81,119 + GFF2033 0.21 -1.2 81,120 - GFF2033 0.21 +0.2 81,323 + GFF2033 0.43 -0.8 81,323 + GFF2033 0.43 +0.4 81,323 + GFF2033 0.43 +0.2 81,323 + GFF2033 0.43 +0.0 81,324 - GFF2033 0.43 +0.2 81,324 - GFF2033 0.43 -0.2 81,324 - GFF2033 0.43 -0.5 81,837 + -0.5 81,837 + -2.5 81,837 + +0.0 81,837 + -0.0
Or see this region's nucleotide sequence