Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF193 and GFF194 are separated by 35 nucleotides GFF194 and GFF195 are separated by 0 nucleotides GFF195 and GFF196 are separated by 105 nucleotides GFF196 and GFF197 are separated by 26 nucleotides
GFF193 - Two-component sensor PilS, at 222,064 to 223,569
GFF193
GFF194 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 223,605 to 224,699
GFF194
GFF195 - RidA/YER057c/UK114 superfamily, group 1, at 224,700 to 225,191
GFF195
GFF196 - Probable ferredoxin subunit of a ring-hydroxylating dioxygenase oxidoreductase protein (EC 1.-.-.-), at 225,297 to 225,617
GFF196
GFF197 - BUG/TctC family periplasmic protein, at 225,644 to 226,612
GFF197
Position (kb) 223
224
225 Strain fitness (log2 ratio) -1
0
1
2 at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.138 kb on + strand, within GFF193 at 223.139 kb on - strand, within GFF193 at 223.160 kb on + strand, within GFF193 at 223.403 kb on + strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.642 kb on + strand at 223.642 kb on + strand at 223.642 kb on + strand at 223.643 kb on - strand at 223.643 kb on - strand at 223.774 kb on + strand, within GFF194 at 223.775 kb on - strand, within GFF194 at 223.775 kb on - strand, within GFF194 at 223.830 kb on + strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.867 kb on - strand, within GFF194 at 223.954 kb on - strand, within GFF194 at 223.977 kb on + strand, within GFF194 at 223.977 kb on + strand, within GFF194 at 224.494 kb on - strand, within GFF194 at 224.514 kb on + strand, within GFF194 at 224.515 kb on - strand, within GFF194 at 224.515 kb on - strand, within GFF194 at 224.767 kb on + strand, within GFF195 at 224.767 kb on + strand, within GFF195 at 224.767 kb on + strand, within GFF195 at 224.768 kb on - strand, within GFF195 at 225.067 kb on + strand, within GFF195 at 225.067 kb on + strand, within GFF195 at 225.067 kb on + strand, within GFF195 at 225.067 kb on + strand, within GFF195 at 225.067 kb on + strand, within GFF195 at 225.068 kb on - strand, within GFF195 at 225.068 kb on - strand, within GFF195 at 225.068 kb on - strand, within GFF195 at 225.068 kb on - strand, within GFF195 at 225.100 kb on + strand, within GFF195 at 225.100 kb on + strand, within GFF195 at 225.100 kb on + strand, within GFF195 at 225.100 kb on + strand, within GFF195 at 225.101 kb on - strand, within GFF195 at 225.101 kb on - strand, within GFF195 at 225.101 kb on - strand, within GFF195 at 225.101 kb on - strand, within GFF195 at 225.191 kb on + strand at 225.191 kb on + strand at 225.191 kb on + strand at 225.191 kb on + strand at 225.192 kb on - strand at 225.192 kb on - strand at 225.192 kb on - strand at 225.192 kb on - strand at 225.192 kb on - strand at 225.247 kb on + strand at 225.247 kb on + strand at 225.248 kb on - strand at 225.248 kb on - strand at 225.346 kb on + strand, within GFF196 at 225.479 kb on + strand, within GFF196 at 225.479 kb on + strand, within GFF196 at 225.479 kb on + strand, within GFF196 at 225.480 kb on - strand, within GFF196 at 225.520 kb on + strand, within GFF196
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 223,127 + GFF193 0.71 -0.7 223,127 + GFF193 0.71 +0.5 223,127 + GFF193 0.71 -0.7 223,127 + GFF193 0.71 +0.5 223,128 - GFF193 0.71 -0.3 223,128 - GFF193 0.71 +0.1 223,128 - GFF193 0.71 -0.6 223,128 - GFF193 0.71 +0.8 223,138 + GFF193 0.71 -1.7 223,139 - GFF193 0.71 -1.1 223,160 + GFF193 0.73 -0.2 223,403 + GFF193 0.89 -0.7 223,404 - GFF193 0.89 -0.1 223,404 - GFF193 0.89 -0.6 223,404 - GFF193 0.89 +0.6 223,642 + -0.4 223,642 + +0.0 223,642 + -0.2 223,643 - +0.5 223,643 - -0.3 223,774 + GFF194 0.15 -0.4 223,775 - GFF194 0.16 -0.5 223,775 - GFF194 0.16 -0.5 223,830 + GFF194 0.21 -0.6 223,831 - GFF194 0.21 +1.3 223,831 - GFF194 0.21 -0.3 223,831 - GFF194 0.21 +0.7 223,867 - GFF194 0.24 -0.0 223,954 - GFF194 0.32 +0.1 223,977 + GFF194 0.34 -0.3 223,977 + GFF194 0.34 -0.7 224,494 - GFF194 0.81 -1.7 224,514 + GFF194 0.83 +0.1 224,515 - GFF194 0.83 -1.4 224,515 - GFF194 0.83 +0.7 224,767 + GFF195 0.14 +1.0 224,767 + GFF195 0.14 +0.9 224,767 + GFF195 0.14 +0.3 224,768 - GFF195 0.14 +0.2 225,067 + GFF195 0.75 +0.3 225,067 + GFF195 0.75 -0.3 225,067 + GFF195 0.75 -1.7 225,067 + GFF195 0.75 -0.7 225,067 + GFF195 0.75 +0.4 225,068 - GFF195 0.75 +0.4 225,068 - GFF195 0.75 -0.7 225,068 - GFF195 0.75 -0.0 225,068 - GFF195 0.75 -0.7 225,100 + GFF195 0.81 +0.6 225,100 + GFF195 0.81 -0.1 225,100 + GFF195 0.81 +0.1 225,100 + GFF195 0.81 +1.9 225,101 - GFF195 0.82 +1.9 225,101 - GFF195 0.82 -0.3 225,101 - GFF195 0.82 +0.4 225,101 - GFF195 0.82 +0.3 225,191 + -0.1 225,191 + +0.3 225,191 + +0.1 225,191 + -0.4 225,192 - +0.8 225,192 - -0.5 225,192 - -0.0 225,192 - +0.3 225,192 - -0.2 225,247 + -0.8 225,247 + -0.3 225,248 - +0.4 225,248 - +0.3 225,346 + GFF196 0.15 -0.0 225,479 + GFF196 0.57 -0.8 225,479 + GFF196 0.57 -0.7 225,479 + GFF196 0.57 +0.3 225,480 - GFF196 0.57 -0.1 225,520 + GFF196 0.69 -0.5
Or see this region's nucleotide sequence