Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1923 and GFF1924 are separated by 31 nucleotides GFF1924 and GFF1925 are separated by 60 nucleotides GFF1925 and GFF1926 overlap by 4 nucleotides
GFF1923 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 603,388 to 604,416
GFF1923
GFF1924 - Aliphatic sulfonate monooxygenase family, FMNH2- or F420-dependent, at 604,448 to 605,569
GFF1924
GFF1925 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 605,630 to 606,454
GFF1925
GFF1926 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 606,451 to 607,458
GFF1926
Position (kb) 604
605
606 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 603.549 kb on + strand, within GFF1923 at 603.553 kb on + strand, within GFF1923 at 603.553 kb on + strand, within GFF1923 at 603.553 kb on + strand, within GFF1923 at 603.553 kb on + strand, within GFF1923 at 603.553 kb on + strand, within GFF1923 at 603.553 kb on + strand, within GFF1923 at 603.554 kb on - strand, within GFF1923 at 603.554 kb on - strand, within GFF1923 at 603.554 kb on - strand, within GFF1923 at 603.554 kb on - strand, within GFF1923 at 603.554 kb on - strand, within GFF1923 at 603.608 kb on - strand, within GFF1923 at 603.841 kb on + strand, within GFF1923 at 603.842 kb on - strand, within GFF1923 at 603.842 kb on - strand, within GFF1923 at 603.865 kb on + strand, within GFF1923 at 603.866 kb on - strand, within GFF1923 at 603.868 kb on + strand, within GFF1923 at 603.868 kb on + strand, within GFF1923 at 603.869 kb on - strand, within GFF1923 at 603.869 kb on - strand, within GFF1923 at 603.869 kb on - strand, within GFF1923 at 603.988 kb on + strand, within GFF1923 at 603.989 kb on - strand, within GFF1923 at 603.989 kb on - strand, within GFF1923 at 603.989 kb on - strand, within GFF1923 at 603.989 kb on - strand, within GFF1923 at 603.989 kb on - strand, within GFF1923 at 603.995 kb on - strand, within GFF1923 at 604.078 kb on + strand, within GFF1923 at 604.078 kb on + strand, within GFF1923 at 604.079 kb on - strand, within GFF1923 at 604.079 kb on - strand, within GFF1923 at 604.186 kb on + strand, within GFF1923 at 604.186 kb on + strand, within GFF1923 at 604.186 kb on + strand, within GFF1923 at 604.186 kb on + strand, within GFF1923 at 604.186 kb on + strand, within GFF1923 at 604.186 kb on + strand, within GFF1923 at 604.187 kb on - strand, within GFF1923 at 604.187 kb on - strand, within GFF1923 at 604.554 kb on - strand at 604.781 kb on + strand, within GFF1924 at 604.782 kb on - strand, within GFF1924 at 604.782 kb on - strand, within GFF1924 at 604.847 kb on + strand, within GFF1924 at 604.865 kb on + strand, within GFF1924 at 604.866 kb on - strand, within GFF1924 at 604.866 kb on - strand, within GFF1924 at 604.887 kb on + strand, within GFF1924 at 604.887 kb on + strand, within GFF1924 at 605.000 kb on + strand, within GFF1924 at 605.000 kb on + strand, within GFF1924 at 605.001 kb on - strand, within GFF1924 at 605.045 kb on + strand, within GFF1924 at 605.046 kb on - strand, within GFF1924 at 605.046 kb on - strand, within GFF1924 at 605.252 kb on + strand, within GFF1924 at 605.429 kb on + strand, within GFF1924 at 605.429 kb on + strand, within GFF1924 at 605.432 kb on + strand, within GFF1924 at 605.433 kb on - strand, within GFF1924 at 605.434 kb on - strand, within GFF1924 at 605.496 kb on - strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.620 kb on - strand at 605.620 kb on - strand at 605.620 kb on - strand at 605.620 kb on - strand at 605.702 kb on + strand at 605.702 kb on + strand at 605.703 kb on - strand at 606.044 kb on + strand, within GFF1925 at 606.045 kb on - strand, within GFF1925 at 606.269 kb on + strand, within GFF1925 at 606.269 kb on + strand, within GFF1925 at 606.270 kb on - strand, within GFF1925
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 603,549 + GFF1923 0.16 -0.9 603,553 + GFF1923 0.16 +0.2 603,553 + GFF1923 0.16 -3.0 603,553 + GFF1923 0.16 -1.3 603,553 + GFF1923 0.16 -0.0 603,553 + GFF1923 0.16 -0.0 603,553 + GFF1923 0.16 +0.2 603,554 - GFF1923 0.16 -0.6 603,554 - GFF1923 0.16 -0.3 603,554 - GFF1923 0.16 +0.4 603,554 - GFF1923 0.16 -0.7 603,554 - GFF1923 0.16 -0.2 603,608 - GFF1923 0.21 +0.5 603,841 + GFF1923 0.44 -0.8 603,842 - GFF1923 0.44 -0.1 603,842 - GFF1923 0.44 -0.7 603,865 + GFF1923 0.46 -0.7 603,866 - GFF1923 0.46 -0.6 603,868 + GFF1923 0.47 +0.3 603,868 + GFF1923 0.47 +0.2 603,869 - GFF1923 0.47 -0.1 603,869 - GFF1923 0.47 -1.2 603,869 - GFF1923 0.47 +0.2 603,988 + GFF1923 0.58 -0.7 603,989 - GFF1923 0.58 -0.1 603,989 - GFF1923 0.58 -1.7 603,989 - GFF1923 0.58 -1.1 603,989 - GFF1923 0.58 -0.4 603,989 - GFF1923 0.58 -0.3 603,995 - GFF1923 0.59 -1.2 604,078 + GFF1923 0.67 +0.3 604,078 + GFF1923 0.67 -0.9 604,079 - GFF1923 0.67 -0.6 604,079 - GFF1923 0.67 -0.6 604,186 + GFF1923 0.78 +1.2 604,186 + GFF1923 0.78 -0.9 604,186 + GFF1923 0.78 +0.0 604,186 + GFF1923 0.78 +0.2 604,186 + GFF1923 0.78 -0.3 604,186 + GFF1923 0.78 +0.6 604,187 - GFF1923 0.78 -1.1 604,187 - GFF1923 0.78 -2.0 604,554 - -0.8 604,781 + GFF1924 0.30 +0.7 604,782 - GFF1924 0.30 -0.6 604,782 - GFF1924 0.30 -1.4 604,847 + GFF1924 0.36 -0.7 604,865 + GFF1924 0.37 -0.1 604,866 - GFF1924 0.37 +0.1 604,866 - GFF1924 0.37 +1.7 604,887 + GFF1924 0.39 -0.5 604,887 + GFF1924 0.39 -0.7 605,000 + GFF1924 0.49 -1.3 605,000 + GFF1924 0.49 +0.3 605,001 - GFF1924 0.49 -0.3 605,045 + GFF1924 0.53 +0.3 605,046 - GFF1924 0.53 -1.5 605,046 - GFF1924 0.53 -0.3 605,252 + GFF1924 0.72 +0.1 605,429 + GFF1924 0.87 -1.2 605,429 + GFF1924 0.87 -0.8 605,432 + GFF1924 0.88 -1.2 605,433 - GFF1924 0.88 -0.7 605,434 - GFF1924 0.88 -0.2 605,496 - -0.3 605,619 + -0.3 605,619 + +0.2 605,619 + +1.4 605,619 + -0.3 605,619 + -1.1 605,619 + -0.3 605,619 + -0.9 605,620 - -0.1 605,620 - +0.1 605,620 - +0.6 605,620 - +0.2 605,702 + -0.8 605,702 + +0.2 605,703 - -1.4 606,044 + GFF1925 0.50 +0.2 606,045 - GFF1925 0.50 -0.4 606,269 + GFF1925 0.77 +0.3 606,269 + GFF1925 0.77 -0.1 606,270 - GFF1925 0.78 -0.7
Or see this region's nucleotide sequence