Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1868 and GFF1869 are separated by 59 nucleotides GFF1869 and GFF1870 overlap by 4 nucleotides GFF1870 and GFF1871 are separated by 13 nucleotides
GFF1868 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 542,873 to 543,715
GFF1868
GFF1869 - no description, at 543,775 to 544,248
GFF1869
GFF1870 - Coenzyme A transferase, at 544,245 to 545,846
GFF1870
GFF1871 - Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.8.1), at 545,860 to 547,002
GFF1871
Position (kb) 544
545
546 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 543.253 kb on - strand, within GFF1868 at 543.253 kb on - strand, within GFF1868 at 543.333 kb on + strand, within GFF1868 at 543.333 kb on + strand, within GFF1868 at 543.334 kb on - strand, within GFF1868 at 543.334 kb on - strand, within GFF1868 at 543.357 kb on + strand, within GFF1868 at 544.010 kb on + strand, within GFF1869 at 544.011 kb on - strand, within GFF1869 at 544.223 kb on + strand at 544.223 kb on + strand at 544.786 kb on + strand, within GFF1870 at 544.787 kb on - strand, within GFF1870 at 545.293 kb on + strand, within GFF1870 at 545.452 kb on + strand, within GFF1870 at 545.452 kb on + strand, within GFF1870 at 545.453 kb on - strand, within GFF1870 at 545.453 kb on - strand, within GFF1870 at 545.924 kb on + strand at 545.924 kb on + strand at 545.925 kb on - strand at 545.925 kb on - strand at 545.925 kb on - strand at 545.925 kb on - strand at 545.981 kb on + strand, within GFF1871 at 546.218 kb on + strand, within GFF1871 at 546.218 kb on + strand, within GFF1871 at 546.505 kb on + strand, within GFF1871 at 546.506 kb on - strand, within GFF1871 at 546.620 kb on + strand, within GFF1871 at 546.620 kb on + strand, within GFF1871
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 543,253 - GFF1868 0.45 +1.2 543,253 - GFF1868 0.45 +0.0 543,333 + GFF1868 0.55 -0.2 543,333 + GFF1868 0.55 -0.3 543,334 - GFF1868 0.55 -0.6 543,334 - GFF1868 0.55 -0.8 543,357 + GFF1868 0.57 +0.7 544,010 + GFF1869 0.50 -1.3 544,011 - GFF1869 0.50 +0.3 544,223 + -0.1 544,223 + +0.6 544,786 + GFF1870 0.34 -2.2 544,787 - GFF1870 0.34 -0.4 545,293 + GFF1870 0.65 -0.0 545,452 + GFF1870 0.75 +0.0 545,452 + GFF1870 0.75 -0.2 545,453 - GFF1870 0.75 -0.6 545,453 - GFF1870 0.75 -1.5 545,924 + -2.0 545,924 + -0.7 545,925 - +1.3 545,925 - +0.8 545,925 - -0.8 545,925 - +1.3 545,981 + GFF1871 0.11 -2.8 546,218 + GFF1871 0.31 -0.8 546,218 + GFF1871 0.31 -0.8 546,505 + GFF1871 0.56 -0.7 546,506 - GFF1871 0.57 -0.8 546,620 + GFF1871 0.66 -0.0 546,620 + GFF1871 0.66 -0.2
Or see this region's nucleotide sequence