Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1678 and GFF1679 are separated by 123 nucleotides GFF1679 and GFF1680 are separated by 62 nucleotides GFF1680 and GFF1681 are separated by 39 nucleotides GFF1681 and GFF1682 are separated by 1 nucleotides
GFF1678 - Transcriptional regulator, GntR family, at 323,423 to 324,214
GFF1678
GFF1679 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 324,338 to 325,042
GFF1679
GFF1680 - KEGG: GntR family transcriptional regulator, at 325,105 to 325,986
GFF1680
GFF1681 - Methylisocitrate lyase (EC 4.1.3.30), at 326,026 to 326,898
GFF1681
GFF1682 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 326,900 to 328,300
GFF1682
Position (kb)
325
326 Strain fitness (log2 ratio)
-1
0
1
2 at 324.177 kb on + strand at 324.178 kb on - strand at 324.190 kb on - strand at 324.627 kb on + strand, within GFF1679 at 324.628 kb on - strand, within GFF1679 at 324.628 kb on - strand, within GFF1679 at 324.645 kb on + strand, within GFF1679 at 324.645 kb on + strand, within GFF1679 at 324.645 kb on + strand, within GFF1679 at 324.646 kb on - strand, within GFF1679 at 324.646 kb on - strand, within GFF1679 at 324.814 kb on - strand, within GFF1679 at 325.031 kb on - strand at 325.077 kb on + strand at 325.078 kb on - strand at 325.123 kb on + strand at 325.123 kb on + strand at 325.124 kb on - strand at 325.199 kb on + strand, within GFF1680 at 325.199 kb on + strand, within GFF1680 at 325.199 kb on + strand, within GFF1680 at 325.199 kb on + strand, within GFF1680 at 325.200 kb on - strand, within GFF1680 at 325.200 kb on - strand, within GFF1680 at 325.200 kb on - strand, within GFF1680 at 325.200 kb on - strand, within GFF1680 at 325.334 kb on - strand, within GFF1680 at 325.377 kb on - strand, within GFF1680 at 325.377 kb on - strand, within GFF1680 at 326.092 kb on + strand at 326.144 kb on - strand, within GFF1681 at 326.282 kb on - strand, within GFF1681 at 326.698 kb on + strand, within GFF1681 at 326.699 kb on - strand, within GFF1681
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 324,177 + +0.2 324,178 - -0.4 324,190 - -1.0 324,627 + GFF1679 0.41 +0.6 324,628 - GFF1679 0.41 -1.2 324,628 - GFF1679 0.41 -1.0 324,645 + GFF1679 0.44 +0.8 324,645 + GFF1679 0.44 +0.7 324,645 + GFF1679 0.44 +1.0 324,646 - GFF1679 0.44 -0.8 324,646 - GFF1679 0.44 -0.4 324,814 - GFF1679 0.68 +0.2 325,031 - +1.8 325,077 + +0.1 325,078 - +0.2 325,123 + -0.3 325,123 + -0.4 325,124 - -0.2 325,199 + GFF1680 0.11 -0.2 325,199 + GFF1680 0.11 +0.9 325,199 + GFF1680 0.11 -1.2 325,199 + GFF1680 0.11 -1.1 325,200 - GFF1680 0.11 +0.1 325,200 - GFF1680 0.11 -0.4 325,200 - GFF1680 0.11 +0.5 325,200 - GFF1680 0.11 -0.6 325,334 - GFF1680 0.26 -0.2 325,377 - GFF1680 0.31 +0.1 325,377 - GFF1680 0.31 -0.0 326,092 + -0.1 326,144 - GFF1681 0.14 -0.1 326,282 - GFF1681 0.29 -0.6 326,698 + GFF1681 0.77 -0.5 326,699 - GFF1681 0.77 +2.2
Or see this region's nucleotide sequence