Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1669 and GFF1670 overlap by 11 nucleotides GFF1670 and GFF1671 overlap by 4 nucleotides GFF1671 and GFF1672 overlap by 4 nucleotides GFF1672 and GFF1673 are separated by 30 nucleotides
GFF1669 - hypothetical protein, at 314,732 to 316,075
GFF1669
GFF1670 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 316,065 to 316,592
GFF1670
GFF1671 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 316,589 to 317,863
GFF1671
GFF1672 - BUG/TctC family periplasmic protein, at 317,860 to 318,825
GFF1672
GFF1673 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 318,856 to 319,566
GFF1673
Position (kb) 316
317
318 Strain fitness (log2 ratio) -1
0
1
2 at 316.316 kb on - strand, within GFF1670 at 316.316 kb on - strand, within GFF1670 at 316.665 kb on + strand at 316.666 kb on - strand at 316.965 kb on + strand, within GFF1671 at 316.992 kb on + strand, within GFF1671 at 317.451 kb on + strand, within GFF1671 at 317.451 kb on + strand, within GFF1671 at 317.452 kb on - strand, within GFF1671 at 317.452 kb on - strand, within GFF1671 at 317.452 kb on - strand, within GFF1671 at 317.565 kb on + strand, within GFF1671 at 317.566 kb on - strand, within GFF1671 at 317.649 kb on + strand, within GFF1671 at 317.650 kb on - strand, within GFF1671 at 317.650 kb on - strand, within GFF1671 at 317.650 kb on - strand, within GFF1671 at 317.879 kb on + strand at 318.279 kb on - strand, within GFF1672 at 318.279 kb on - strand, within GFF1672 at 318.279 kb on - strand, within GFF1672 at 318.315 kb on - strand, within GFF1672
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 316,316 - GFF1670 0.48 -0.5 316,316 - GFF1670 0.48 +0.2 316,665 + -1.1 316,666 - +0.2 316,965 + GFF1671 0.29 -0.5 316,992 + GFF1671 0.32 -0.9 317,451 + GFF1671 0.68 +0.3 317,451 + GFF1671 0.68 -1.7 317,452 - GFF1671 0.68 +0.3 317,452 - GFF1671 0.68 -0.5 317,452 - GFF1671 0.68 -1.1 317,565 + GFF1671 0.77 +1.8 317,566 - GFF1671 0.77 -1.2 317,649 + GFF1671 0.83 +1.8 317,650 - GFF1671 0.83 +0.3 317,650 - GFF1671 0.83 -0.1 317,650 - GFF1671 0.83 -1.5 317,879 + -0.5 318,279 - GFF1672 0.43 +1.0 318,279 - GFF1672 0.43 -1.0 318,279 - GFF1672 0.43 -0.4 318,315 - GFF1672 0.47 +0.3
Or see this region's nucleotide sequence