Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1630 and GFF1631 overlap by 19 nucleotides GFF1631 and GFF1632 are separated by 136 nucleotides GFF1632 and GFF1633 are separated by 8 nucleotides
GFF1630 - Esterase/lipase, at 230,544 to 232,628
GFF1630
GFF1631 - L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) protein, at 232,610 to 233,926
GFF1631
GFF1632 - RNA polymerase ECF-type sigma factor, at 234,063 to 234,695
GFF1632
GFF1633 - Probable transmembrane sensor, at 234,704 to 235,771
GFF1633
Position (kb) 232
233
234 Strain fitness (log2 ratio) -2
-1
0
1
2 at 231.927 kb on + strand, within GFF1630 at 231.927 kb on + strand, within GFF1630 at 231.927 kb on + strand, within GFF1630 at 231.987 kb on + strand, within GFF1630 at 232.138 kb on - strand, within GFF1630 at 232.210 kb on - strand, within GFF1630 at 232.356 kb on + strand, within GFF1630 at 232.356 kb on + strand, within GFF1630 at 232.357 kb on - strand, within GFF1630 at 232.922 kb on + strand, within GFF1631 at 232.923 kb on - strand, within GFF1631 at 232.923 kb on - strand, within GFF1631 at 233.165 kb on + strand, within GFF1631 at 233.166 kb on - strand, within GFF1631 at 233.477 kb on + strand, within GFF1631 at 233.613 kb on - strand, within GFF1631 at 233.639 kb on + strand, within GFF1631 at 233.639 kb on + strand, within GFF1631 at 234.088 kb on + strand at 234.088 kb on + strand at 234.097 kb on - strand at 234.507 kb on + strand, within GFF1632 at 234.508 kb on - strand, within GFF1632 at 234.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 231,927 + GFF1630 0.66 -0.2 231,927 + GFF1630 0.66 -0.4 231,927 + GFF1630 0.66 -0.3 231,987 + GFF1630 0.69 -0.5 232,138 - GFF1630 0.76 -0.2 232,210 - GFF1630 0.80 -1.6 232,356 + GFF1630 0.87 -0.5 232,356 + GFF1630 0.87 -1.7 232,357 - GFF1630 0.87 -0.8 232,922 + GFF1631 0.24 -0.0 232,923 - GFF1631 0.24 -2.2 232,923 - GFF1631 0.24 -0.7 233,165 + GFF1631 0.42 -0.8 233,166 - GFF1631 0.42 -2.0 233,477 + GFF1631 0.66 -0.2 233,613 - GFF1631 0.76 -0.3 233,639 + GFF1631 0.78 +1.8 233,639 + GFF1631 0.78 +0.2 234,088 + -2.3 234,088 + -0.6 234,097 - -0.4 234,507 + GFF1632 0.70 +0.8 234,508 - GFF1632 0.70 -1.1 234,668 + +0.0
Or see this region's nucleotide sequence