Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1628 and GFF1629 are separated by 177 nucleotides GFF1629 and GFF1630 are separated by 65 nucleotides GFF1630 and GFF1631 overlap by 19 nucleotides
GFF1628 - no description, at 229,309 to 229,548
GFF1628
GFF1629 - Ferric reductase (1.6.99.14), at 229,726 to 230,478
GFF1629
GFF1630 - Esterase/lipase, at 230,544 to 232,628
GFF1630
GFF1631 - L-lysine 6-monooxygenase (Lysine N(6)-hydroxylase) protein, at 232,610 to 233,926
GFF1631
Position (kb) 230
231
232
233 Strain fitness (log2 ratio) -2
-1
0
1 at 229.939 kb on + strand, within GFF1629 at 230.062 kb on - strand, within GFF1629 at 230.404 kb on + strand at 230.405 kb on - strand at 230.405 kb on - strand at 230.405 kb on - strand at 230.407 kb on + strand at 230.407 kb on + strand at 230.408 kb on - strand at 230.428 kb on + strand at 230.428 kb on + strand at 230.510 kb on + strand at 230.510 kb on + strand at 230.652 kb on + strand at 230.797 kb on - strand, within GFF1630 at 230.815 kb on - strand, within GFF1630 at 230.815 kb on - strand, within GFF1630 at 231.109 kb on - strand, within GFF1630 at 231.109 kb on - strand, within GFF1630 at 231.207 kb on + strand, within GFF1630 at 231.208 kb on - strand, within GFF1630 at 231.306 kb on + strand, within GFF1630 at 231.336 kb on + strand, within GFF1630 at 231.336 kb on + strand, within GFF1630 at 231.337 kb on - strand, within GFF1630 at 231.927 kb on + strand, within GFF1630 at 231.927 kb on + strand, within GFF1630 at 231.927 kb on + strand, within GFF1630 at 231.987 kb on + strand, within GFF1630 at 232.138 kb on - strand, within GFF1630 at 232.210 kb on - strand, within GFF1630 at 232.356 kb on + strand, within GFF1630 at 232.356 kb on + strand, within GFF1630 at 232.357 kb on - strand, within GFF1630 at 232.922 kb on + strand, within GFF1631 at 232.923 kb on - strand, within GFF1631 at 232.923 kb on - strand, within GFF1631 at 233.165 kb on + strand, within GFF1631 at 233.166 kb on - strand, within GFF1631 at 233.477 kb on + strand, within GFF1631 at 233.613 kb on - strand, within GFF1631
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 229,939 + GFF1629 0.28 -2.0 230,062 - GFF1629 0.45 +0.5 230,404 + -0.5 230,405 - -1.3 230,405 - +0.6 230,405 - -1.3 230,407 + +1.0 230,407 + +1.0 230,408 - -0.1 230,428 + +0.8 230,428 + +0.5 230,510 + -0.5 230,510 + -0.4 230,652 + -1.4 230,797 - GFF1630 0.12 +0.3 230,815 - GFF1630 0.13 -1.0 230,815 - GFF1630 0.13 -1.0 231,109 - GFF1630 0.27 +1.3 231,109 - GFF1630 0.27 -0.6 231,207 + GFF1630 0.32 -1.4 231,208 - GFF1630 0.32 -0.2 231,306 + GFF1630 0.37 -1.0 231,336 + GFF1630 0.38 -0.3 231,336 + GFF1630 0.38 +0.0 231,337 - GFF1630 0.38 -0.5 231,927 + GFF1630 0.66 -0.2 231,927 + GFF1630 0.66 -0.4 231,927 + GFF1630 0.66 -0.3 231,987 + GFF1630 0.69 -0.5 232,138 - GFF1630 0.76 -0.2 232,210 - GFF1630 0.80 -1.6 232,356 + GFF1630 0.87 -0.5 232,356 + GFF1630 0.87 -1.7 232,357 - GFF1630 0.87 -0.8 232,922 + GFF1631 0.24 -0.0 232,923 - GFF1631 0.24 -2.2 232,923 - GFF1631 0.24 -0.7 233,165 + GFF1631 0.42 -0.8 233,166 - GFF1631 0.42 -2.0 233,477 + GFF1631 0.66 -0.2 233,613 - GFF1631 0.76 -0.3
Or see this region's nucleotide sequence