Strain Fitness in Variovorax sp. SCN45 around GFF1509

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1507 and GFF1508 overlap by 4 nucleotidesGFF1508 and GFF1509 overlap by 4 nucleotidesGFF1509 and GFF1510 overlap by 4 nucleotides GFF1507 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 99,377 to 101,443 GFF1507 GFF1508 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 101,440 to 102,339 GFF1508 GFF1509 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 102,336 to 103,310 GFF1509 GFF1510 - Oxidoreductase, at 103,307 to 104,797 GFF1510 Position (kb) 102 103 104Strain fitness (log2 ratio) -2 -1 0 1 2at 101.546 kb on + strand, within GFF1508at 101.547 kb on - strand, within GFF1508at 101.570 kb on + strand, within GFF1508at 101.771 kb on + strand, within GFF1508at 101.771 kb on + strand, within GFF1508at 101.771 kb on + strand, within GFF1508at 101.771 kb on + strand, within GFF1508at 101.820 kb on - strand, within GFF1508at 101.820 kb on - strand, within GFF1508at 101.822 kb on + strand, within GFF1508at 101.822 kb on + strand, within GFF1508at 101.822 kb on + strand, within GFF1508at 101.823 kb on - strand, within GFF1508at 101.879 kb on + strand, within GFF1508at 101.879 kb on + strand, within GFF1508at 101.879 kb on + strand, within GFF1508at 101.879 kb on + strand, within GFF1508at 101.880 kb on - strand, within GFF1508at 101.880 kb on - strand, within GFF1508at 101.880 kb on - strand, within GFF1508at 102.074 kb on + strand, within GFF1508at 102.110 kb on + strand, within GFF1508at 102.111 kb on - strand, within GFF1508at 102.111 kb on - strand, within GFF1508at 102.116 kb on + strand, within GFF1508at 102.116 kb on + strand, within GFF1508at 102.116 kb on + strand, within GFF1508at 102.117 kb on - strand, within GFF1508at 102.117 kb on - strand, within GFF1508at 102.185 kb on + strand, within GFF1508at 102.186 kb on - strand, within GFF1508at 102.321 kb on - strandat 102.321 kb on - strandat 102.379 kb on + strandat 102.380 kb on - strandat 102.380 kb on - strandat 102.380 kb on - strandat 102.436 kb on + strand, within GFF1509at 102.437 kb on - strand, within GFF1509at 102.437 kb on - strand, within GFF1509at 102.437 kb on - strand, within GFF1509at 102.655 kb on + strand, within GFF1509at 102.656 kb on - strand, within GFF1509at 102.656 kb on - strand, within GFF1509at 102.656 kb on - strand, within GFF1509at 103.120 kb on + strand, within GFF1509at 103.351 kb on - strandat 103.404 kb on + strandat 103.404 kb on + strandat 103.405 kb on - strandat 103.542 kb on + strand, within GFF1510at 103.542 kb on + strand, within GFF1510at 103.543 kb on - strand, within GFF1510at 103.543 kb on - strand, within GFF1510at 103.923 kb on + strand, within GFF1510at 103.923 kb on + strand, within GFF1510at 103.923 kb on + strand, within GFF1510at 103.923 kb on + strand, within GFF1510at 103.923 kb on + strand, within GFF1510at 103.923 kb on + strand, within GFF1510at 103.923 kb on + strand, within GFF1510at 103.924 kb on - strand, within GFF1510at 103.924 kb on - strand, within GFF1510at 103.924 kb on - strand, within GFF1510at 103.924 kb on - strand, within GFF1510at 104.268 kb on + strand, within GFF1510at 104.268 kb on + strand, within GFF1510at 104.268 kb on + strand, within GFF1510at 104.268 kb on + strand, within GFF1510at 104.269 kb on - strand, within GFF1510

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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101,546 + GFF1508 0.12 -0.4
101,547 - GFF1508 0.12 +1.5
101,570 + GFF1508 0.14 +0.2
101,771 + GFF1508 0.37 -1.4
101,771 + GFF1508 0.37 -1.0
101,771 + GFF1508 0.37 +0.6
101,771 + GFF1508 0.37 +0.4
101,820 - GFF1508 0.42 -1.2
101,820 - GFF1508 0.42 +0.2
101,822 + GFF1508 0.42 -0.0
101,822 + GFF1508 0.42 -0.3
101,822 + GFF1508 0.42 +0.7
101,823 - GFF1508 0.43 +0.7
101,879 + GFF1508 0.49 -0.6
101,879 + GFF1508 0.49 +1.1
101,879 + GFF1508 0.49 -0.8
101,879 + GFF1508 0.49 +0.1
101,880 - GFF1508 0.49 +0.0
101,880 - GFF1508 0.49 -1.4
101,880 - GFF1508 0.49 -1.3
102,074 + GFF1508 0.70 +2.4
102,110 + GFF1508 0.74 -0.3
102,111 - GFF1508 0.75 +0.2
102,111 - GFF1508 0.75 -0.8
102,116 + GFF1508 0.75 -1.0
102,116 + GFF1508 0.75 +0.2
102,116 + GFF1508 0.75 +0.0
102,117 - GFF1508 0.75 +0.2
102,117 - GFF1508 0.75 -0.8
102,185 + GFF1508 0.83 -0.3
102,186 - GFF1508 0.83 -0.5
102,321 - -0.1
102,321 - -0.3
102,379 + +1.1
102,380 - +1.4
102,380 - +0.8
102,380 - +1.0
102,436 + GFF1509 0.10 -0.4
102,437 - GFF1509 0.10 -0.2
102,437 - GFF1509 0.10 -0.5
102,437 - GFF1509 0.10 -0.1
102,655 + GFF1509 0.33 -0.3
102,656 - GFF1509 0.33 -1.8
102,656 - GFF1509 0.33 -0.8
102,656 - GFF1509 0.33 -0.7
103,120 + GFF1509 0.80 +0.1
103,351 - +0.6
103,404 + -0.2
103,404 + -0.7
103,405 - -0.8
103,542 + GFF1510 0.16 -0.1
103,542 + GFF1510 0.16 -0.8
103,543 - GFF1510 0.16 +0.2
103,543 - GFF1510 0.16 +1.9
103,923 + GFF1510 0.41 -0.7
103,923 + GFF1510 0.41 +0.6
103,923 + GFF1510 0.41 +0.1
103,923 + GFF1510 0.41 -1.0
103,923 + GFF1510 0.41 -1.4
103,923 + GFF1510 0.41 -0.2
103,923 + GFF1510 0.41 -1.1
103,924 - GFF1510 0.41 -0.2
103,924 - GFF1510 0.41 -0.0
103,924 - GFF1510 0.41 -0.4
103,924 - GFF1510 0.41 +0.2
104,268 + GFF1510 0.64 +0.4
104,268 + GFF1510 0.64 -0.1
104,268 + GFF1510 0.64 -0.3
104,268 + GFF1510 0.64 -0.4
104,269 - GFF1510 0.65 -1.1

Or see this region's nucleotide sequence