Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1425 and GFF1426 are separated by 10 nucleotides GFF1426 and GFF1427 are separated by 7 nucleotides GFF1427 and GFF1428 overlap by 4 nucleotides GFF1428 and GFF1429 are separated by 126 nucleotides GFF1429 and GFF1430 are separated by 116 nucleotides
GFF1425 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 16,225 to 16,974
GFF1425
GFF1426 - Phenylacetate ABC transporter, ATP-binding protein 2, at 16,985 to 17,704
GFF1426
GFF1427 - Mandelate racemase (EC 5.1.2.2), at 17,712 to 18,788
GFF1427
GFF1428 - no description, at 18,785 to 18,940
GFF1428
GFF1429 - no description, at 19,067 to 19,438
GFF1429
GFF1430 - hypothetical protein, at 19,555 to 21,840
GFF1430
Position (kb) 17
18
19 Strain fitness (log2 ratio) -2
-1
0
1
2 at 16.988 kb on + strand at 17.351 kb on + strand, within GFF1426 at 17.352 kb on - strand, within GFF1426 at 17.352 kb on - strand, within GFF1426 at 17.352 kb on - strand, within GFF1426 at 17.352 kb on - strand, within GFF1426 at 17.612 kb on + strand, within GFF1426 at 17.612 kb on + strand, within GFF1426 at 17.612 kb on + strand, within GFF1426 at 17.613 kb on - strand, within GFF1426 at 17.690 kb on + strand at 17.690 kb on + strand at 17.822 kb on - strand, within GFF1427 at 17.859 kb on + strand, within GFF1427 at 17.859 kb on + strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.872 kb on - strand, within GFF1427 at 17.967 kb on + strand, within GFF1427 at 17.968 kb on - strand, within GFF1427 at 18.436 kb on + strand, within GFF1427 at 18.600 kb on - strand, within GFF1427 at 18.970 kb on + strand at 18.971 kb on - strand at 18.971 kb on - strand at 18.971 kb on - strand at 19.222 kb on - strand, within GFF1429 at 19.222 kb on - strand, within GFF1429 at 19.305 kb on + strand, within GFF1429 at 19.305 kb on + strand, within GFF1429 at 19.608 kb on + strand at 19.609 kb on - strand at 19.609 kb on - strand at 19.609 kb on - strand at 19.698 kb on - strand at 19.698 kb on - strand at 19.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 16,988 + +0.4 17,351 + GFF1426 0.51 -2.0 17,352 - GFF1426 0.51 -0.0 17,352 - GFF1426 0.51 -1.7 17,352 - GFF1426 0.51 +1.0 17,352 - GFF1426 0.51 -2.3 17,612 + GFF1426 0.87 +0.3 17,612 + GFF1426 0.87 +0.2 17,612 + GFF1426 0.87 +0.7 17,613 - GFF1426 0.87 +0.5 17,690 + +0.3 17,690 + -2.1 17,822 - GFF1427 0.10 -0.0 17,859 + GFF1427 0.14 +0.8 17,859 + GFF1427 0.14 +0.7 17,860 - GFF1427 0.14 +2.3 17,860 - GFF1427 0.14 +0.1 17,860 - GFF1427 0.14 +1.2 17,872 - GFF1427 0.15 -0.3 17,967 + GFF1427 0.24 +0.3 17,968 - GFF1427 0.24 -0.2 18,436 + GFF1427 0.67 -0.5 18,600 - GFF1427 0.82 -0.6 18,970 + +0.2 18,971 - -0.4 18,971 - -2.0 18,971 - +0.2 19,222 - GFF1429 0.42 +0.7 19,222 - GFF1429 0.42 -0.1 19,305 + GFF1429 0.64 +2.0 19,305 + GFF1429 0.64 +1.7 19,608 + +0.2 19,609 - -0.8 19,609 - -1.8 19,609 - -0.7 19,698 - -0.1 19,698 - +0.2 19,698 - -0.0
Or see this region's nucleotide sequence