Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1243 and GFF1244 are separated by 113 nucleotides GFF1244 and GFF1245 are separated by 22 nucleotides GFF1245 and GFF1246 are separated by 7 nucleotides GFF1246 and GFF1247 are separated by 137 nucleotides
GFF1243 - transglutaminase, N-terminal domain protein, at 562,939 to 563,847
GFF1243
GFF1244 - Uncharacterized protein YqjZ, at 563,961 to 564,293
GFF1244
GFF1245 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 564,316 to 566,157
GFF1245
GFF1246 - FIG001341: Probable Fe(2+)-trafficking protein YggX, at 566,165 to 566,437
GFF1246
GFF1247 - Sulfate and thiosulfate binding protein CysP, at 566,575 to 567,615
GFF1247
Position (kb) 564
565
566
567 Strain fitness (log2 ratio) -2
-1
0
1
2 at 563.354 kb on + strand, within GFF1243 at 563.354 kb on + strand, within GFF1243 at 563.354 kb on + strand, within GFF1243 at 563.354 kb on + strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.492 kb on + strand, within GFF1243 at 563.492 kb on + strand, within GFF1243 at 563.492 kb on + strand, within GFF1243 at 563.493 kb on - strand, within GFF1243 at 563.493 kb on - strand, within GFF1243 at 563.493 kb on - strand, within GFF1243 at 563.795 kb on + strand at 563.796 kb on - strand at 563.802 kb on - strand at 563.825 kb on + strand at 563.825 kb on + strand at 563.825 kb on + strand at 563.825 kb on + strand at 563.964 kb on + strand at 563.964 kb on + strand at 563.964 kb on + strand at 563.964 kb on + strand at 563.964 kb on + strand at 563.965 kb on - strand at 563.965 kb on - strand at 563.965 kb on - strand at 563.965 kb on - strand at 563.965 kb on - strand at 563.965 kb on - strand at 563.965 kb on - strand at 563.965 kb on - strand at 563.965 kb on - strand at 563.965 kb on - strand at 564.060 kb on + strand, within GFF1244 at 564.114 kb on + strand, within GFF1244 at 564.114 kb on + strand, within GFF1244 at 564.114 kb on + strand, within GFF1244 at 564.114 kb on + strand, within GFF1244 at 564.115 kb on - strand, within GFF1244 at 564.115 kb on - strand, within GFF1244 at 564.115 kb on - strand, within GFF1244 at 564.115 kb on - strand, within GFF1244 at 564.115 kb on - strand, within GFF1244 at 564.117 kb on + strand, within GFF1244 at 564.117 kb on + strand, within GFF1244 at 564.117 kb on + strand, within GFF1244 at 564.117 kb on + strand, within GFF1244 at 564.117 kb on + strand, within GFF1244 at 564.118 kb on - strand, within GFF1244 at 564.118 kb on - strand, within GFF1244 at 564.118 kb on - strand, within GFF1244 at 564.118 kb on - strand, within GFF1244 at 564.118 kb on - strand, within GFF1244 at 564.118 kb on - strand, within GFF1244 at 564.118 kb on - strand, within GFF1244 at 564.207 kb on + strand, within GFF1244 at 564.207 kb on + strand, within GFF1244 at 564.207 kb on + strand, within GFF1244 at 564.207 kb on + strand, within GFF1244 at 564.208 kb on - strand, within GFF1244 at 564.208 kb on - strand, within GFF1244 at 564.208 kb on - strand, within GFF1244 at 564.240 kb on + strand, within GFF1244 at 564.240 kb on + strand, within GFF1244 at 564.240 kb on + strand, within GFF1244 at 564.241 kb on - strand, within GFF1244 at 564.241 kb on - strand, within GFF1244 at 564.241 kb on - strand, within GFF1244 at 564.241 kb on - strand, within GFF1244 at 564.241 kb on - strand, within GFF1244 at 564.241 kb on - strand, within GFF1244 at 564.241 kb on - strand, within GFF1244 at 564.892 kb on + strand, within GFF1245 at 564.892 kb on + strand, within GFF1245 at 564.893 kb on - strand, within GFF1245 at 564.893 kb on - strand, within GFF1245 at 564.893 kb on - strand, within GFF1245 at 564.893 kb on - strand, within GFF1245 at 564.893 kb on - strand, within GFF1245 at 564.923 kb on - strand, within GFF1245 at 565.517 kb on + strand, within GFF1245 at 565.517 kb on + strand, within GFF1245 at 565.517 kb on + strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.518 kb on - strand, within GFF1245 at 565.538 kb on + strand, within GFF1245 at 565.539 kb on - strand, within GFF1245 at 565.539 kb on - strand, within GFF1245 at 565.844 kb on + strand, within GFF1245 at 565.845 kb on - strand, within GFF1245 at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.090 kb on + strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.091 kb on - strand at 566.093 kb on - strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.184 kb on + strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.244 kb on + strand, within GFF1246 at 566.244 kb on + strand, within GFF1246 at 566.244 kb on + strand, within GFF1246 at 566.244 kb on + strand, within GFF1246 at 566.245 kb on - strand, within GFF1246 at 566.245 kb on - strand, within GFF1246 at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.470 kb on + strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.471 kb on - strand at 566.483 kb on + strand at 566.483 kb on + strand at 566.487 kb on + strand at 566.487 kb on + strand at 566.488 kb on - strand at 566.516 kb on + strand at 566.518 kb on - strand at 566.518 kb on - strand at 566.519 kb on + strand at 566.519 kb on + strand at 566.520 kb on - strand at 566.520 kb on - strand at 566.520 kb on - strand at 566.526 kb on + strand at 566.527 kb on - strand at 566.535 kb on + strand at 566.535 kb on + strand at 566.535 kb on + strand at 566.552 kb on - strand at 566.552 kb on - strand at 566.561 kb on + strand at 566.562 kb on - strand at 566.595 kb on + strand at 566.595 kb on + strand at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.701 kb on + strand, within GFF1247 at 566.702 kb on - strand, within GFF1247 at 566.702 kb on - strand, within GFF1247 at 566.702 kb on - strand, within GFF1247 at 566.702 kb on - strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.722 kb on + strand, within GFF1247 at 566.723 kb on - strand, within GFF1247 at 566.723 kb on - strand, within GFF1247 at 566.723 kb on - strand, within GFF1247 at 566.723 kb on - strand, within GFF1247 at 566.731 kb on + strand, within GFF1247 at 566.731 kb on + strand, within GFF1247 at 566.731 kb on + strand, within GFF1247 at 566.732 kb on - strand, within GFF1247 at 566.732 kb on - strand, within GFF1247 at 566.732 kb on - strand, within GFF1247 at 566.732 kb on - strand, within GFF1247 at 566.752 kb on + strand, within GFF1247 at 566.752 kb on + strand, within GFF1247 at 566.753 kb on - strand, within GFF1247 at 566.753 kb on - strand, within GFF1247 at 566.753 kb on - strand, within GFF1247 at 566.753 kb on - strand, within GFF1247 at 566.953 kb on + strand, within GFF1247 at 566.953 kb on + strand, within GFF1247 at 566.953 kb on + strand, within GFF1247 at 566.953 kb on + strand, within GFF1247 at 566.954 kb on - strand, within GFF1247 at 566.954 kb on - strand, within GFF1247 at 566.954 kb on - strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.085 kb on + strand, within GFF1247 at 567.086 kb on - strand, within GFF1247 at 567.086 kb on - strand, within GFF1247 at 567.118 kb on + strand, within GFF1247 at 567.118 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.145 kb on + strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247 at 567.146 kb on - strand, within GFF1247
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 563,354 + GFF1243 0.46 +1.0 563,354 + GFF1243 0.46 +1.4 563,354 + GFF1243 0.46 -0.2 563,354 + GFF1243 0.46 +0.4 563,355 - GFF1243 0.46 -0.3 563,355 - GFF1243 0.46 -0.7 563,355 - GFF1243 0.46 -0.5 563,355 - GFF1243 0.46 -0.7 563,355 - GFF1243 0.46 -0.6 563,492 + GFF1243 0.61 -0.9 563,492 + GFF1243 0.61 +0.5 563,492 + GFF1243 0.61 -0.4 563,493 - GFF1243 0.61 -1.7 563,493 - GFF1243 0.61 +1.4 563,493 - GFF1243 0.61 +0.1 563,795 + -0.2 563,796 - +1.6 563,802 - -0.0 563,825 + -0.6 563,825 + -0.2 563,825 + +0.1 563,825 + +0.2 563,964 + -0.4 563,964 + +0.0 563,964 + -1.1 563,964 + -1.1 563,964 + +0.0 563,965 - -0.5 563,965 - -0.2 563,965 - +0.8 563,965 - -0.5 563,965 - +1.1 563,965 - -0.4 563,965 - -0.3 563,965 - -0.3 563,965 - -0.2 563,965 - -1.1 564,060 + GFF1244 0.30 +1.2 564,114 + GFF1244 0.46 -0.5 564,114 + GFF1244 0.46 +0.0 564,114 + GFF1244 0.46 -0.9 564,114 + GFF1244 0.46 +0.5 564,115 - GFF1244 0.46 -0.2 564,115 - GFF1244 0.46 +0.9 564,115 - GFF1244 0.46 -0.7 564,115 - GFF1244 0.46 -0.6 564,115 - GFF1244 0.46 +0.7 564,117 + GFF1244 0.47 +1.9 564,117 + GFF1244 0.47 -1.2 564,117 + GFF1244 0.47 -0.1 564,117 + GFF1244 0.47 -0.5 564,117 + GFF1244 0.47 -0.5 564,118 - GFF1244 0.47 -1.2 564,118 - GFF1244 0.47 +0.3 564,118 - GFF1244 0.47 +0.4 564,118 - GFF1244 0.47 -0.6 564,118 - GFF1244 0.47 +0.1 564,118 - GFF1244 0.47 -0.3 564,118 - GFF1244 0.47 +0.8 564,207 + GFF1244 0.74 +0.1 564,207 + GFF1244 0.74 -0.6 564,207 + GFF1244 0.74 -0.9 564,207 + GFF1244 0.74 +0.5 564,208 - GFF1244 0.74 -0.0 564,208 - GFF1244 0.74 -2.1 564,208 - GFF1244 0.74 -0.6 564,240 + GFF1244 0.84 -0.9 564,240 + GFF1244 0.84 -0.1 564,240 + GFF1244 0.84 -0.6 564,241 - GFF1244 0.84 -0.3 564,241 - GFF1244 0.84 -0.2 564,241 - GFF1244 0.84 -1.0 564,241 - GFF1244 0.84 -0.6 564,241 - GFF1244 0.84 +0.6 564,241 - GFF1244 0.84 -0.4 564,241 - GFF1244 0.84 -1.1 564,892 + GFF1245 0.31 +0.8 564,892 + GFF1245 0.31 -1.3 564,893 - GFF1245 0.31 +0.5 564,893 - GFF1245 0.31 -0.5 564,893 - GFF1245 0.31 +0.3 564,893 - GFF1245 0.31 +0.5 564,893 - GFF1245 0.31 -2.3 564,923 - GFF1245 0.33 -0.2 565,517 + GFF1245 0.65 +0.5 565,517 + GFF1245 0.65 -1.6 565,517 + GFF1245 0.65 +0.1 565,518 - GFF1245 0.65 -0.9 565,518 - GFF1245 0.65 +0.4 565,518 - GFF1245 0.65 +0.2 565,518 - GFF1245 0.65 -0.3 565,518 - GFF1245 0.65 +1.0 565,518 - GFF1245 0.65 +0.9 565,518 - GFF1245 0.65 -0.8 565,518 - GFF1245 0.65 -1.6 565,518 - GFF1245 0.65 -0.7 565,518 - GFF1245 0.65 +0.5 565,518 - GFF1245 0.65 +1.4 565,538 + GFF1245 0.66 -1.8 565,539 - GFF1245 0.66 -0.7 565,539 - GFF1245 0.66 +0.4 565,844 + GFF1245 0.83 -0.1 565,845 - GFF1245 0.83 -0.5 566,090 + -0.7 566,090 + +0.9 566,090 + -0.4 566,090 + -0.8 566,090 + +0.3 566,090 + +0.0 566,090 + -0.0 566,090 + -2.6 566,090 + -1.8 566,090 + -0.2 566,090 + -0.7 566,090 + -0.4 566,090 + -0.9 566,090 + -0.6 566,090 + -0.4 566,090 + -0.2 566,090 + +0.4 566,090 + -0.1 566,090 + -0.9 566,090 + -0.5 566,091 - +0.6 566,091 - -0.2 566,091 - -1.0 566,091 - -0.1 566,091 - -0.6 566,091 - +0.2 566,091 - -0.8 566,091 - -0.2 566,091 - -0.4 566,091 - +0.4 566,091 - -0.5 566,091 - -1.2 566,091 - -0.2 566,091 - -1.4 566,091 - +0.1 566,091 - +0.3 566,091 - -0.1 566,091 - -0.1 566,091 - -0.2 566,093 - -0.0 566,184 + +0.1 566,184 + +0.5 566,184 + +1.1 566,184 + +1.4 566,184 + -1.3 566,184 + -1.9 566,184 + -0.7 566,184 + -0.9 566,184 + -0.4 566,185 - -0.1 566,185 - -2.1 566,185 - +0.7 566,185 - -0.0 566,185 - -0.1 566,185 - -0.6 566,185 - -0.8 566,185 - -0.5 566,185 - +0.0 566,185 - -0.3 566,185 - -1.8 566,244 + GFF1246 0.29 -0.1 566,244 + GFF1246 0.29 -1.6 566,244 + GFF1246 0.29 -0.9 566,244 + GFF1246 0.29 +0.0 566,245 - GFF1246 0.29 +0.5 566,245 - GFF1246 0.29 -0.8 566,470 + +0.4 566,470 + -0.9 566,470 + -0.8 566,470 + +0.1 566,470 + -0.9 566,470 + -0.6 566,470 + -1.4 566,470 + +0.0 566,470 + +1.0 566,470 + +0.2 566,470 + +0.4 566,471 - +1.0 566,471 - +0.9 566,471 - +0.1 566,471 - -1.6 566,471 - -1.8 566,471 - +0.8 566,483 + -0.2 566,483 + -0.1 566,487 + -0.5 566,487 + -0.5 566,488 - -0.6 566,516 + +0.2 566,518 - -0.5 566,518 - -1.4 566,519 + +0.7 566,519 + +0.8 566,520 - +0.1 566,520 - -1.6 566,520 - +0.7 566,526 + +0.2 566,527 - -0.2 566,535 + +0.5 566,535 + -0.1 566,535 + +0.6 566,552 - -0.7 566,552 - -0.8 566,561 + +1.2 566,562 - -1.8 566,595 + +0.2 566,595 + -0.6 566,701 + GFF1247 0.12 -1.5 566,701 + GFF1247 0.12 +0.6 566,701 + GFF1247 0.12 -0.7 566,701 + GFF1247 0.12 -0.6 566,701 + GFF1247 0.12 +0.6 566,701 + GFF1247 0.12 -0.5 566,701 + GFF1247 0.12 -0.3 566,702 - GFF1247 0.12 -1.1 566,702 - GFF1247 0.12 +0.2 566,702 - GFF1247 0.12 -0.6 566,702 - GFF1247 0.12 -0.1 566,722 + GFF1247 0.14 +0.2 566,722 + GFF1247 0.14 -0.7 566,722 + GFF1247 0.14 -0.2 566,722 + GFF1247 0.14 +0.1 566,722 + GFF1247 0.14 +0.7 566,722 + GFF1247 0.14 -1.4 566,722 + GFF1247 0.14 +0.4 566,722 + GFF1247 0.14 +0.1 566,722 + GFF1247 0.14 +0.0 566,722 + GFF1247 0.14 -0.1 566,723 - GFF1247 0.14 +2.0 566,723 - GFF1247 0.14 +0.6 566,723 - GFF1247 0.14 -0.8 566,723 - GFF1247 0.14 +0.0 566,731 + GFF1247 0.15 -0.6 566,731 + GFF1247 0.15 -0.6 566,731 + GFF1247 0.15 -0.7 566,732 - GFF1247 0.15 -0.1 566,732 - GFF1247 0.15 -0.3 566,732 - GFF1247 0.15 +1.0 566,732 - GFF1247 0.15 +0.1 566,752 + GFF1247 0.17 -0.0 566,752 + GFF1247 0.17 -0.3 566,753 - GFF1247 0.17 -1.2 566,753 - GFF1247 0.17 +1.4 566,753 - GFF1247 0.17 +0.4 566,753 - GFF1247 0.17 +0.9 566,953 + GFF1247 0.36 +0.1 566,953 + GFF1247 0.36 -0.5 566,953 + GFF1247 0.36 -0.5 566,953 + GFF1247 0.36 -0.8 566,954 - GFF1247 0.36 -0.6 566,954 - GFF1247 0.36 -0.7 566,954 - GFF1247 0.36 -0.0 567,085 + GFF1247 0.49 -0.8 567,085 + GFF1247 0.49 +0.3 567,085 + GFF1247 0.49 +0.8 567,085 + GFF1247 0.49 -0.1 567,085 + GFF1247 0.49 -0.4 567,085 + GFF1247 0.49 -0.2 567,086 - GFF1247 0.49 -0.4 567,086 - GFF1247 0.49 +1.4 567,118 + GFF1247 0.52 +0.9 567,118 + GFF1247 0.52 -0.0 567,145 + GFF1247 0.55 -1.0 567,145 + GFF1247 0.55 -1.3 567,145 + GFF1247 0.55 +2.4 567,145 + GFF1247 0.55 -0.6 567,145 + GFF1247 0.55 +0.6 567,145 + GFF1247 0.55 +0.4 567,146 - GFF1247 0.55 +0.1 567,146 - GFF1247 0.55 -2.1 567,146 - GFF1247 0.55 -1.8 567,146 - GFF1247 0.55 +0.2 567,146 - GFF1247 0.55 -1.9 567,146 - GFF1247 0.55 +0.2
Or see this region's nucleotide sequence