Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF109 and GFF110 are separated by 10 nucleotides GFF110 and GFF111 overlap by 4 nucleotides
GFF109 - Protein lysine acetyltransferase Pat (EC 2.3.1.-), at 136,527 to 139,229
GFF109
GFF110 - pyridoxal-phosphate dependent enzyme family protein, at 139,240 to 140,199
GFF110
GFF111 - hypothetical protein, at 140,196 to 141,221
GFF111
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.283 kb on - strand, within GFF109 at 138.283 kb on - strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.879 kb on + strand, within GFF109 at 138.978 kb on + strand at 138.978 kb on + strand at 138.979 kb on - strand at 138.979 kb on - strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.227 kb on + strand at 139.442 kb on + strand, within GFF110 at 139.442 kb on + strand, within GFF110 at 139.443 kb on - strand, within GFF110 at 139.443 kb on - strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.554 kb on - strand, within GFF110 at 139.745 kb on + strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.929 kb on - strand, within GFF110 at 140.121 kb on + strand at 140.132 kb on + strand at 140.132 kb on + strand at 140.278 kb on + strand at 140.278 kb on + strand at 140.278 kb on + strand at 140.279 kb on - strand at 140.279 kb on - strand at 140.404 kb on + strand, within GFF111 at 140.404 kb on + strand, within GFF111 at 140.794 kb on + strand, within GFF111 at 140.794 kb on + strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 141.028 kb on + strand, within GFF111
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 138,280 + GFF109 0.65 +0.6 138,280 + GFF109 0.65 -0.8 138,280 + GFF109 0.65 +0.7 138,280 + GFF109 0.65 -0.2 138,280 + GFF109 0.65 +0.7 138,281 - GFF109 0.65 -0.3 138,281 - GFF109 0.65 -0.5 138,281 - GFF109 0.65 +0.5 138,282 + GFF109 0.65 +0.5 138,282 + GFF109 0.65 +0.2 138,282 + GFF109 0.65 +0.6 138,282 + GFF109 0.65 -0.5 138,282 + GFF109 0.65 +0.2 138,283 - GFF109 0.65 -2.0 138,283 - GFF109 0.65 -1.0 138,732 + GFF109 0.82 -1.2 138,732 + GFF109 0.82 +1.3 138,732 + GFF109 0.82 -1.0 138,732 + GFF109 0.82 -1.2 138,732 + GFF109 0.82 +0.5 138,733 - GFF109 0.82 +0.5 138,733 - GFF109 0.82 -0.0 138,733 - GFF109 0.82 -0.4 138,879 + GFF109 0.87 +2.1 138,978 + +0.6 138,978 + +1.1 138,979 - +0.5 138,979 - -0.5 139,218 + -0.2 139,218 + +0.7 139,218 + -0.0 139,218 + +0.2 139,218 + -0.9 139,219 - +0.3 139,219 - +0.3 139,219 - +0.4 139,219 - -1.3 139,219 - -0.2 139,227 + -0.3 139,442 + GFF110 0.21 -0.3 139,442 + GFF110 0.21 -0.1 139,443 - GFF110 0.21 -0.1 139,443 - GFF110 0.21 +0.4 139,553 + GFF110 0.33 -0.5 139,553 + GFF110 0.33 -0.0 139,553 + GFF110 0.33 -0.5 139,553 + GFF110 0.33 +1.6 139,554 - GFF110 0.33 +0.0 139,745 + GFF110 0.53 +0.2 139,746 - GFF110 0.53 -0.0 139,746 - GFF110 0.53 +0.2 139,746 - GFF110 0.53 -0.3 139,746 - GFF110 0.53 +0.3 139,928 + GFF110 0.72 +0.1 139,928 + GFF110 0.72 -0.0 139,928 + GFF110 0.72 +1.3 139,929 - GFF110 0.72 -0.8 140,121 + +0.9 140,132 + -1.5 140,132 + -0.7 140,278 + +0.7 140,278 + +0.6 140,278 + -1.0 140,279 - -1.0 140,279 - -0.7 140,404 + GFF111 0.20 -0.0 140,404 + GFF111 0.20 +0.3 140,794 + GFF111 0.58 +0.5 140,794 + GFF111 0.58 -0.6 140,795 - GFF111 0.58 -1.0 140,795 - GFF111 0.58 -0.1 140,795 - GFF111 0.58 +0.2 140,795 - GFF111 0.58 +1.0 141,028 + GFF111 0.81 -0.2
Or see this region's nucleotide sequence