Strain Fitness in Variovorax sp. SCN45 around GFF99

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF98 and GFF99 overlap by 1 nucleotidesGFF99 and GFF100 overlap by 4 nucleotides GFF98 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 123,851 to 124,849 GFF98 GFF99 - KEGG: RNA polymerase sigma-70 factor, ECF subfamily, at 124,849 to 125,409 GFF99 GFF100 - no description, at 125,406 to 126,104 GFF100 Position (kb) 124 125 126Strain fitness (log2 ratio) -1 0 1 2at 124.853 kb on + strandat 124.854 kb on - strandat 124.854 kb on - strandat 125.194 kb on + strand, within GFF99at 125.194 kb on + strand, within GFF99at 125.194 kb on + strand, within GFF99at 125.360 kb on + strandat 125.360 kb on + strandat 125.547 kb on + strand, within GFF100at 125.548 kb on - strand, within GFF100at 125.548 kb on - strand, within GFF100at 125.548 kb on - strand, within GFF100at 125.548 kb on - strand, within GFF100at 125.651 kb on - strand, within GFF100at 125.958 kb on + strand, within GFF100at 125.958 kb on + strand, within GFF100at 125.958 kb on + strand, within GFF100at 125.958 kb on + strand, within GFF100at 125.958 kb on + strand, within GFF100at 125.959 kb on - strand, within GFF100at 125.959 kb on - strand, within GFF100at 125.984 kb on - strand, within GFF100at 126.248 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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124,853 + -0.7
124,854 - +0.1
124,854 - -0.1
125,194 + GFF99 0.61 -1.6
125,194 + GFF99 0.61 +0.1
125,194 + GFF99 0.61 -1.3
125,360 + -1.0
125,360 + -0.0
125,547 + GFF100 0.20 -0.9
125,548 - GFF100 0.20 -0.4
125,548 - GFF100 0.20 +0.1
125,548 - GFF100 0.20 +2.7
125,548 - GFF100 0.20 +0.6
125,651 - GFF100 0.35 +0.1
125,958 + GFF100 0.79 -1.2
125,958 + GFF100 0.79 -0.5
125,958 + GFF100 0.79 +1.3
125,958 + GFF100 0.79 +0.0
125,958 + GFF100 0.79 +0.1
125,959 - GFF100 0.79 -0.6
125,959 - GFF100 0.79 -0.2
125,984 - GFF100 0.83 +0.1
126,248 - -0.8

Or see this region's nucleotide sequence