Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF940 and GFF941 are separated by 112 nucleotides GFF941 and GFF942 overlap by 4 nucleotides GFF942 and GFF943 are separated by 19 nucleotides
GFF940 - Permease of the drug/metabolite transporter (DMT) superfamily, at 253,484 to 254,392
GFF940
GFF941 - Transporter, LysE family, at 254,505 to 255,143
GFF941
GFF942 - Iron-sulfur cluster-binding protein, at 255,140 to 255,775
GFF942
GFF943 - Intracellular PHB depolymerase, at 255,795 to 257,237
GFF943
Position (kb)
255
256 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 254.171 kb on + strand, within GFF940 at 254.171 kb on + strand, within GFF940 at 254.171 kb on + strand, within GFF940 at 254.171 kb on + strand, within GFF940 at 254.171 kb on + strand, within GFF940 at 254.171 kb on + strand, within GFF940 at 254.172 kb on - strand, within GFF940 at 254.172 kb on - strand, within GFF940 at 254.172 kb on - strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.210 kb on + strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.211 kb on - strand, within GFF940 at 254.255 kb on + strand, within GFF940 at 254.255 kb on + strand, within GFF940 at 254.255 kb on + strand, within GFF940 at 254.255 kb on + strand, within GFF940 at 254.255 kb on + strand, within GFF940 at 254.256 kb on - strand, within GFF940 at 254.256 kb on - strand, within GFF940 at 254.256 kb on - strand, within GFF940 at 254.256 kb on - strand, within GFF940 at 254.256 kb on - strand, within GFF940 at 254.261 kb on + strand, within GFF940 at 254.334 kb on - strand at 254.377 kb on - strand at 254.506 kb on + strand at 254.528 kb on + strand at 254.529 kb on - strand at 254.529 kb on - strand at 254.635 kb on + strand, within GFF941 at 254.635 kb on + strand, within GFF941 at 254.635 kb on + strand, within GFF941 at 254.635 kb on + strand, within GFF941 at 254.636 kb on - strand, within GFF941 at 254.636 kb on - strand, within GFF941 at 254.689 kb on + strand, within GFF941 at 254.689 kb on + strand, within GFF941 at 254.689 kb on + strand, within GFF941 at 254.690 kb on - strand, within GFF941 at 254.690 kb on - strand, within GFF941 at 254.690 kb on - strand, within GFF941 at 254.690 kb on - strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.874 kb on + strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.875 kb on - strand, within GFF941 at 254.899 kb on + strand, within GFF941 at 254.899 kb on + strand, within GFF941 at 254.899 kb on + strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.900 kb on - strand, within GFF941 at 254.919 kb on + strand, within GFF941 at 254.920 kb on - strand, within GFF941 at 254.920 kb on - strand, within GFF941 at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.118 kb on + strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.119 kb on - strand at 255.309 kb on + strand, within GFF942 at 255.310 kb on - strand, within GFF942 at 255.310 kb on - strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.693 kb on + strand, within GFF942 at 255.694 kb on - strand, within GFF942 at 255.694 kb on - strand, within GFF942 at 255.694 kb on - strand, within GFF942 at 256.015 kb on + strand, within GFF943 at 256.054 kb on + strand, within GFF943 at 256.054 kb on + strand, within GFF943 at 256.078 kb on + strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.079 kb on - strand, within GFF943 at 256.278 kb on + strand, within GFF943 at 256.278 kb on + strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.279 kb on - strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.300 kb on + strand, within GFF943 at 256.301 kb on - strand, within GFF943 at 256.301 kb on - strand, within GFF943 at 256.301 kb on - strand, within GFF943 at 256.301 kb on - strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.303 kb on + strand, within GFF943 at 256.304 kb on - strand, within GFF943 at 256.304 kb on - strand, within GFF943 at 256.304 kb on - strand, within GFF943 at 256.304 kb on - strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.333 kb on + strand, within GFF943 at 256.334 kb on - strand, within GFF943 at 256.342 kb on + strand, within GFF943 at 256.342 kb on + strand, within GFF943 at 256.342 kb on + strand, within GFF943 at 256.343 kb on - strand, within GFF943 at 256.343 kb on - strand, within GFF943 at 256.343 kb on - strand, within GFF943 at 256.399 kb on + strand, within GFF943 at 256.399 kb on + strand, within GFF943 at 256.400 kb on - strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.405 kb on + strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.406 kb on - strand, within GFF943 at 256.472 kb on - strand, within GFF943 at 256.513 kb on + strand, within GFF943 at 256.513 kb on + strand, within GFF943 at 256.514 kb on - strand, within GFF943 at 256.514 kb on - strand, within GFF943 at 256.514 kb on - strand, within GFF943 at 256.714 kb on + strand, within GFF943 at 256.715 kb on - strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.750 kb on + strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.751 kb on - strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.759 kb on + strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943 at 256.760 kb on - strand, within GFF943
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 254,171 + GFF940 0.76 -0.2 254,171 + GFF940 0.76 -0.2 254,171 + GFF940 0.76 -0.2 254,171 + GFF940 0.76 -0.7 254,171 + GFF940 0.76 +0.4 254,171 + GFF940 0.76 -0.6 254,172 - GFF940 0.76 -0.1 254,172 - GFF940 0.76 +0.0 254,172 - GFF940 0.76 -0.2 254,210 + GFF940 0.80 -0.5 254,210 + GFF940 0.80 +0.5 254,210 + GFF940 0.80 -1.2 254,210 + GFF940 0.80 -0.8 254,210 + GFF940 0.80 +1.4 254,210 + GFF940 0.80 -0.3 254,210 + GFF940 0.80 +1.2 254,210 + GFF940 0.80 +0.2 254,210 + GFF940 0.80 -0.2 254,210 + GFF940 0.80 -0.1 254,211 - GFF940 0.80 -0.3 254,211 - GFF940 0.80 -0.4 254,211 - GFF940 0.80 +0.9 254,211 - GFF940 0.80 -0.5 254,211 - GFF940 0.80 -0.6 254,211 - GFF940 0.80 +0.0 254,211 - GFF940 0.80 -0.4 254,211 - GFF940 0.80 -0.6 254,211 - GFF940 0.80 -0.2 254,211 - GFF940 0.80 +0.1 254,211 - GFF940 0.80 -0.4 254,211 - GFF940 0.80 -0.6 254,255 + GFF940 0.85 -0.2 254,255 + GFF940 0.85 -0.5 254,255 + GFF940 0.85 -1.3 254,255 + GFF940 0.85 +0.6 254,255 + GFF940 0.85 -0.5 254,256 - GFF940 0.85 +0.2 254,256 - GFF940 0.85 +0.4 254,256 - GFF940 0.85 -0.7 254,256 - GFF940 0.85 -0.1 254,256 - GFF940 0.85 +0.5 254,261 + GFF940 0.85 +0.2 254,334 - +0.3 254,377 - -0.2 254,506 + -0.3 254,528 + +0.8 254,529 - -0.2 254,529 - -0.2 254,635 + GFF941 0.20 -0.9 254,635 + GFF941 0.20 -0.6 254,635 + GFF941 0.20 +2.4 254,635 + GFF941 0.20 -0.9 254,636 - GFF941 0.21 -1.0 254,636 - GFF941 0.21 -0.5 254,689 + GFF941 0.29 +0.0 254,689 + GFF941 0.29 -0.0 254,689 + GFF941 0.29 -0.3 254,690 - GFF941 0.29 +0.2 254,690 - GFF941 0.29 -0.2 254,690 - GFF941 0.29 -0.5 254,690 - GFF941 0.29 -0.1 254,874 + GFF941 0.58 +0.2 254,874 + GFF941 0.58 -0.6 254,874 + GFF941 0.58 -0.7 254,874 + GFF941 0.58 -1.2 254,874 + GFF941 0.58 -0.6 254,875 - GFF941 0.58 -0.2 254,875 - GFF941 0.58 -0.4 254,875 - GFF941 0.58 -0.4 254,875 - GFF941 0.58 -0.3 254,875 - GFF941 0.58 +0.0 254,899 + GFF941 0.62 -0.6 254,899 + GFF941 0.62 -0.4 254,899 + GFF941 0.62 +0.3 254,900 - GFF941 0.62 -0.3 254,900 - GFF941 0.62 +1.4 254,900 - GFF941 0.62 +0.3 254,900 - GFF941 0.62 +0.4 254,900 - GFF941 0.62 -0.5 254,900 - GFF941 0.62 +1.3 254,900 - GFF941 0.62 +0.4 254,919 + GFF941 0.65 -0.2 254,920 - GFF941 0.65 -0.1 254,920 - GFF941 0.65 +0.8 255,118 + +0.4 255,118 + -1.2 255,118 + +0.1 255,118 + +0.0 255,118 + -0.0 255,118 + -0.7 255,118 + -0.0 255,118 + +0.4 255,118 + -0.3 255,118 + -1.2 255,118 + +0.0 255,118 + +0.1 255,118 + -0.5 255,118 + +0.1 255,118 + -0.3 255,118 + +1.0 255,118 + +1.4 255,118 + -0.7 255,118 + -0.1 255,118 + -0.6 255,118 + +0.7 255,118 + +0.2 255,118 + +0.2 255,118 + -0.7 255,118 + +0.7 255,119 - +0.5 255,119 - +0.4 255,119 - -0.6 255,119 - -1.2 255,119 - -1.0 255,119 - -0.6 255,119 - +0.2 255,119 - -0.7 255,119 - -0.4 255,119 - +0.5 255,119 - -0.1 255,119 - +0.3 255,119 - +0.5 255,119 - -0.3 255,119 - -0.5 255,119 - -0.3 255,119 - -0.2 255,119 - -0.7 255,119 - -0.1 255,119 - -0.7 255,119 - +0.5 255,119 - -0.6 255,119 - +0.3 255,119 - -0.2 255,309 + GFF942 0.27 -0.2 255,310 - GFF942 0.27 +1.4 255,310 - GFF942 0.27 +0.4 255,693 + GFF942 0.87 -0.1 255,693 + GFF942 0.87 -0.1 255,693 + GFF942 0.87 -0.4 255,693 + GFF942 0.87 -0.4 255,693 + GFF942 0.87 -1.4 255,693 + GFF942 0.87 +0.3 255,693 + GFF942 0.87 -0.2 255,694 - GFF942 0.87 +0.8 255,694 - GFF942 0.87 +1.2 255,694 - GFF942 0.87 +1.4 256,015 + GFF943 0.15 -1.4 256,054 + GFF943 0.18 -1.2 256,054 + GFF943 0.18 -0.8 256,078 + GFF943 0.20 +0.0 256,079 - GFF943 0.20 -1.0 256,079 - GFF943 0.20 -0.7 256,079 - GFF943 0.20 -1.7 256,079 - GFF943 0.20 +0.1 256,079 - GFF943 0.20 -0.4 256,278 + GFF943 0.33 -0.9 256,278 + GFF943 0.33 -0.1 256,279 - GFF943 0.34 -0.4 256,279 - GFF943 0.34 -0.5 256,279 - GFF943 0.34 +0.1 256,279 - GFF943 0.34 -0.8 256,279 - GFF943 0.34 -0.8 256,300 + GFF943 0.35 -0.5 256,300 + GFF943 0.35 -0.5 256,300 + GFF943 0.35 -1.6 256,300 + GFF943 0.35 -1.7 256,300 + GFF943 0.35 +0.8 256,301 - GFF943 0.35 +0.1 256,301 - GFF943 0.35 +0.1 256,301 - GFF943 0.35 -0.4 256,301 - GFF943 0.35 -0.3 256,303 + GFF943 0.35 +0.8 256,303 + GFF943 0.35 -0.1 256,303 + GFF943 0.35 +0.6 256,303 + GFF943 0.35 -0.7 256,303 + GFF943 0.35 +0.4 256,303 + GFF943 0.35 -0.5 256,304 - GFF943 0.35 -1.2 256,304 - GFF943 0.35 -0.3 256,304 - GFF943 0.35 -0.6 256,304 - GFF943 0.35 -0.5 256,333 + GFF943 0.37 -0.9 256,333 + GFF943 0.37 -0.4 256,333 + GFF943 0.37 -0.4 256,333 + GFF943 0.37 -1.0 256,333 + GFF943 0.37 -0.6 256,333 + GFF943 0.37 -1.1 256,333 + GFF943 0.37 -0.8 256,333 + GFF943 0.37 +0.5 256,334 - GFF943 0.37 -0.4 256,342 + GFF943 0.38 -1.2 256,342 + GFF943 0.38 -0.0 256,342 + GFF943 0.38 +1.1 256,343 - GFF943 0.38 -0.6 256,343 - GFF943 0.38 -0.9 256,343 - GFF943 0.38 -0.8 256,399 + GFF943 0.42 +0.1 256,399 + GFF943 0.42 +0.4 256,400 - GFF943 0.42 -1.5 256,405 + GFF943 0.42 -1.0 256,405 + GFF943 0.42 +0.2 256,405 + GFF943 0.42 -1.5 256,405 + GFF943 0.42 -0.4 256,405 + GFF943 0.42 +0.8 256,405 + GFF943 0.42 -1.4 256,405 + GFF943 0.42 -1.6 256,406 - GFF943 0.42 +0.2 256,406 - GFF943 0.42 -0.9 256,406 - GFF943 0.42 -0.6 256,406 - GFF943 0.42 -0.4 256,406 - GFF943 0.42 +2.2 256,406 - GFF943 0.42 +0.0 256,472 - GFF943 0.47 -0.4 256,513 + GFF943 0.50 -1.4 256,513 + GFF943 0.50 -0.5 256,514 - GFF943 0.50 +0.3 256,514 - GFF943 0.50 -2.8 256,514 - GFF943 0.50 -0.6 256,714 + GFF943 0.64 +0.7 256,715 - GFF943 0.64 -0.7 256,750 + GFF943 0.66 -0.5 256,750 + GFF943 0.66 -0.8 256,750 + GFF943 0.66 +0.1 256,750 + GFF943 0.66 -0.4 256,750 + GFF943 0.66 -0.7 256,750 + GFF943 0.66 -1.3 256,750 + GFF943 0.66 -1.0 256,751 - GFF943 0.66 +0.1 256,751 - GFF943 0.66 -0.7 256,751 - GFF943 0.66 -0.5 256,751 - GFF943 0.66 -0.6 256,751 - GFF943 0.66 +0.0 256,751 - GFF943 0.66 +0.0 256,751 - GFF943 0.66 +0.2 256,759 + GFF943 0.67 -1.6 256,759 + GFF943 0.67 +0.3 256,759 + GFF943 0.67 -1.4 256,759 + GFF943 0.67 -1.1 256,759 + GFF943 0.67 -0.6 256,760 - GFF943 0.67 +0.1 256,760 - GFF943 0.67 -0.7 256,760 - GFF943 0.67 +1.4 256,760 - GFF943 0.67 -0.2 256,760 - GFF943 0.67 +0.0 256,760 - GFF943 0.67 +0.0 256,760 - GFF943 0.67 -0.6 256,760 - GFF943 0.67 -1.0
Or see this region's nucleotide sequence