Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF913 and GFF914 overlap by 4 nucleotides GFF914 and GFF915 are separated by 1 nucleotides GFF915 and GFF916 are separated by 18 nucleotides
GFF913 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 224,050 to 225,714
GFF913
GFF914 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 225,711 to 226,526
GFF914
GFF915 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 226,528 to 227,472
GFF915
GFF916 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 227,491 to 229,110
GFF916
Position (kb) 226
227
228 Strain fitness (log2 ratio) -1
0
1
2
3 at 225.835 kb on + strand, within GFF914 at 225.835 kb on + strand, within GFF914 at 225.835 kb on + strand, within GFF914 at 225.836 kb on - strand, within GFF914 at 226.003 kb on + strand, within GFF914 at 226.003 kb on + strand, within GFF914 at 226.004 kb on - strand, within GFF914 at 226.042 kb on + strand, within GFF914 at 226.102 kb on + strand, within GFF914 at 226.102 kb on + strand, within GFF914 at 226.103 kb on - strand, within GFF914 at 226.435 kb on + strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.505 kb on + strand at 226.529 kb on + strand at 226.529 kb on + strand at 226.562 kb on + strand at 226.563 kb on - strand at 226.595 kb on + strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.838 kb on + strand, within GFF915 at 226.838 kb on + strand, within GFF915 at 226.839 kb on - strand, within GFF915 at 226.839 kb on - strand, within GFF915 at 226.934 kb on + strand, within GFF915 at 226.935 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 226.974 kb on - strand, within GFF915 at 227.279 kb on + strand, within GFF915 at 227.279 kb on + strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.280 kb on - strand, within GFF915 at 227.462 kb on + strand at 227.463 kb on - strand at 227.771 kb on + strand, within GFF916 at 227.822 kb on + strand, within GFF916 at 227.838 kb on - strand, within GFF916 at 227.930 kb on + strand, within GFF916 at 228.053 kb on + strand, within GFF916 at 228.053 kb on + strand, within GFF916 at 228.056 kb on + strand, within GFF916 at 228.314 kb on + strand, within GFF916 at 228.426 kb on - strand, within GFF916 at 228.452 kb on + strand, within GFF916 at 228.452 kb on + strand, within GFF916 at 228.453 kb on - strand, within GFF916
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 225,835 + GFF914 0.15 -1.0 225,835 + GFF914 0.15 -1.1 225,835 + GFF914 0.15 +0.2 225,836 - GFF914 0.15 -0.2 226,003 + GFF914 0.36 -0.9 226,003 + GFF914 0.36 -1.6 226,004 - GFF914 0.36 +0.7 226,042 + GFF914 0.41 -0.4 226,102 + GFF914 0.48 -0.5 226,102 + GFF914 0.48 -0.7 226,103 - GFF914 0.48 +1.0 226,435 + GFF914 0.89 -0.3 226,436 - GFF914 0.89 +0.0 226,436 - GFF914 0.89 -0.4 226,436 - GFF914 0.89 +1.8 226,436 - GFF914 0.89 +1.1 226,436 - GFF914 0.89 -1.1 226,436 - GFF914 0.89 -0.8 226,436 - GFF914 0.89 -0.1 226,436 - GFF914 0.89 -1.3 226,505 + +2.8 226,529 + +0.7 226,529 + +1.3 226,562 + +0.4 226,563 - -0.1 226,595 + -0.8 226,596 - +0.9 226,596 - -0.3 226,596 - -0.3 226,596 - -0.5 226,838 + GFF915 0.33 +0.2 226,838 + GFF915 0.33 -1.2 226,839 - GFF915 0.33 -0.1 226,839 - GFF915 0.33 -0.1 226,934 + GFF915 0.43 -0.2 226,935 - GFF915 0.43 -0.8 226,974 - GFF915 0.47 -1.5 226,974 - GFF915 0.47 -0.4 226,974 - GFF915 0.47 -0.6 227,279 + GFF915 0.79 -0.3 227,279 + GFF915 0.79 -0.1 227,280 - GFF915 0.80 -0.3 227,280 - GFF915 0.80 -1.5 227,280 - GFF915 0.80 -0.2 227,280 - GFF915 0.80 -0.5 227,462 + -0.5 227,463 - +0.4 227,771 + GFF916 0.17 -1.2 227,822 + GFF916 0.20 +0.6 227,838 - GFF916 0.21 +1.6 227,930 + GFF916 0.27 +0.1 228,053 + GFF916 0.35 -0.3 228,053 + GFF916 0.35 -0.1 228,056 + GFF916 0.35 +0.2 228,314 + GFF916 0.51 +0.1 228,426 - GFF916 0.58 +0.3 228,452 + GFF916 0.59 -0.2 228,452 + GFF916 0.59 -0.8 228,453 - GFF916 0.59 +0.8
Or see this region's nucleotide sequence