Strain Fitness in Variovorax sp. SCN45 around GFF898

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF897 and GFF898 are separated by 7 nucleotidesGFF898 and GFF899 are separated by 14 nucleotides GFF897 - putative oxygenase subunit, at 207,317 to 208,558 GFF897 GFF898 - putative oxygenase subunit, at 208,566 to 210,299 GFF898 GFF899 - Dienelactone hydrolase family protein, at 210,314 to 211,546 GFF899 Position (kb) 208 209 210 211Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 207.842 kb on + strand, within GFF897at 207.842 kb on + strand, within GFF897at 207.842 kb on + strand, within GFF897at 207.843 kb on - strand, within GFF897at 207.914 kb on + strand, within GFF897at 207.915 kb on - strand, within GFF897at 208.265 kb on + strand, within GFF897at 208.349 kb on + strand, within GFF897at 208.526 kb on + strandat 208.527 kb on - strandat 208.527 kb on - strandat 208.656 kb on + strandat 209.088 kb on + strand, within GFF898at 209.088 kb on + strand, within GFF898at 209.089 kb on - strand, within GFF898at 209.089 kb on - strand, within GFF898at 209.089 kb on - strand, within GFF898at 209.161 kb on - strand, within GFF898at 209.511 kb on + strand, within GFF898at 209.511 kb on + strand, within GFF898at 209.511 kb on + strand, within GFF898at 209.511 kb on + strand, within GFF898at 210.171 kb on + strandat 210.172 kb on - strandat 210.172 kb on - strandat 210.172 kb on - strandat 210.258 kb on + strandat 210.259 kb on - strandat 210.259 kb on - strandat 210.261 kb on + strandat 210.261 kb on + strandat 210.261 kb on + strandat 210.262 kb on - strandat 210.262 kb on - strandat 210.297 kb on + strandat 210.297 kb on + strandat 210.297 kb on + strandat 210.298 kb on - strandat 210.344 kb on + strandat 210.344 kb on + strandat 210.344 kb on + strandat 210.344 kb on + strandat 210.344 kb on + strandat 210.344 kb on + strandat 210.345 kb on - strandat 210.345 kb on - strandat 210.345 kb on - strandat 210.345 kb on - strandat 210.345 kb on - strandat 210.482 kb on + strand, within GFF899at 210.483 kb on - strand, within GFF899at 210.483 kb on - strand, within GFF899at 210.485 kb on + strand, within GFF899at 210.486 kb on - strand, within GFF899at 210.486 kb on - strand, within GFF899at 210.486 kb on - strand, within GFF899at 210.500 kb on + strand, within GFF899at 210.500 kb on + strand, within GFF899at 210.500 kb on + strand, within GFF899at 210.501 kb on - strand, within GFF899at 210.501 kb on - strand, within GFF899at 210.501 kb on - strand, within GFF899at 210.501 kb on - strand, within GFF899at 210.501 kb on - strand, within GFF899at 210.558 kb on - strand, within GFF899at 210.558 kb on - strand, within GFF899at 210.593 kb on + strand, within GFF899at 210.593 kb on + strand, within GFF899at 210.593 kb on + strand, within GFF899at 210.594 kb on - strand, within GFF899at 210.723 kb on - strand, within GFF899at 210.764 kb on + strand, within GFF899at 210.764 kb on + strand, within GFF899at 210.767 kb on + strand, within GFF899at 210.767 kb on + strand, within GFF899at 210.768 kb on - strand, within GFF899at 210.917 kb on + strand, within GFF899at 210.917 kb on + strand, within GFF899at 210.917 kb on + strand, within GFF899at 210.917 kb on + strand, within GFF899at 210.917 kb on + strand, within GFF899at 210.918 kb on - strand, within GFF899at 210.918 kb on - strand, within GFF899at 210.918 kb on - strand, within GFF899at 210.918 kb on - strand, within GFF899at 210.918 kb on - strand, within GFF899at 210.918 kb on - strand, within GFF899at 210.923 kb on + strand, within GFF899at 211.040 kb on + strand, within GFF899at 211.040 kb on + strand, within GFF899at 211.040 kb on + strand, within GFF899at 211.040 kb on + strand, within GFF899at 211.077 kb on - strand, within GFF899at 211.133 kb on + strand, within GFF899at 211.133 kb on + strand, within GFF899at 211.134 kb on - strand, within GFF899at 211.134 kb on - strand, within GFF899at 211.172 kb on + strand, within GFF899at 211.173 kb on - strand, within GFF899at 211.173 kb on - strand, within GFF899at 211.193 kb on + strand, within GFF899at 211.193 kb on + strand, within GFF899at 211.194 kb on - strand, within GFF899at 211.194 kb on - strand, within GFF899at 211.194 kb on - strand, within GFF899at 211.194 kb on - strand, within GFF899at 211.195 kb on + strand, within GFF899at 211.195 kb on + strand, within GFF899at 211.196 kb on - strand, within GFF899at 211.297 kb on + strand, within GFF899at 211.298 kb on - strand, within GFF899

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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207,842 + GFF897 0.42 +0.2
207,842 + GFF897 0.42 +0.3
207,842 + GFF897 0.42 -1.1
207,843 - GFF897 0.42 +0.8
207,914 + GFF897 0.48 -0.1
207,915 - GFF897 0.48 -2.1
208,265 + GFF897 0.76 +0.1
208,349 + GFF897 0.83 -0.8
208,526 + +0.7
208,527 - +0.8
208,527 - -0.1
208,656 + -0.9
209,088 + GFF898 0.30 +0.9
209,088 + GFF898 0.30 +0.3
209,089 - GFF898 0.30 +0.1
209,089 - GFF898 0.30 -0.9
209,089 - GFF898 0.30 -1.1
209,161 - GFF898 0.34 -1.7
209,511 + GFF898 0.54 -1.4
209,511 + GFF898 0.54 -2.0
209,511 + GFF898 0.54 -0.9
209,511 + GFF898 0.54 -0.2
210,171 + +0.1
210,172 - +1.0
210,172 - -0.7
210,172 - -1.3
210,258 + -0.5
210,259 - +0.4
210,259 - +0.1
210,261 + -0.5
210,261 + -0.6
210,261 + -0.6
210,262 - -1.2
210,262 - +0.4
210,297 + +1.1
210,297 + -0.4
210,297 + +0.6
210,298 - +1.0
210,344 + -0.1
210,344 + +0.7
210,344 + +0.2
210,344 + +0.4
210,344 + -0.4
210,344 + +0.6
210,345 - +0.1
210,345 - -0.2
210,345 - -0.7
210,345 - -0.1
210,345 - -0.3
210,482 + GFF899 0.14 -1.1
210,483 - GFF899 0.14 -0.0
210,483 - GFF899 0.14 +0.0
210,485 + GFF899 0.14 +0.4
210,486 - GFF899 0.14 -0.3
210,486 - GFF899 0.14 +0.1
210,486 - GFF899 0.14 +0.1
210,500 + GFF899 0.15 -0.3
210,500 + GFF899 0.15 -0.9
210,500 + GFF899 0.15 -0.6
210,501 - GFF899 0.15 -0.6
210,501 - GFF899 0.15 +1.3
210,501 - GFF899 0.15 -0.1
210,501 - GFF899 0.15 +0.3
210,501 - GFF899 0.15 -0.0
210,558 - GFF899 0.20 +1.0
210,558 - GFF899 0.20 -0.3
210,593 + GFF899 0.23 -0.8
210,593 + GFF899 0.23 +0.4
210,593 + GFF899 0.23 +2.0
210,594 - GFF899 0.23 -0.5
210,723 - GFF899 0.33 +0.1
210,764 + GFF899 0.36 -1.1
210,764 + GFF899 0.36 +1.4
210,767 + GFF899 0.37 -1.0
210,767 + GFF899 0.37 +0.1
210,768 - GFF899 0.37 -0.4
210,917 + GFF899 0.49 -1.6
210,917 + GFF899 0.49 -2.8
210,917 + GFF899 0.49 -1.3
210,917 + GFF899 0.49 -0.0
210,917 + GFF899 0.49 -0.3
210,918 - GFF899 0.49 +1.4
210,918 - GFF899 0.49 -1.6
210,918 - GFF899 0.49 +0.3
210,918 - GFF899 0.49 +0.4
210,918 - GFF899 0.49 +0.1
210,918 - GFF899 0.49 -0.6
210,923 + GFF899 0.49 -0.4
211,040 + GFF899 0.59 +0.3
211,040 + GFF899 0.59 -1.4
211,040 + GFF899 0.59 -1.3
211,040 + GFF899 0.59 +0.8
211,077 - GFF899 0.62 -1.1
211,133 + GFF899 0.66 -0.8
211,133 + GFF899 0.66 +0.1
211,134 - GFF899 0.67 +1.1
211,134 - GFF899 0.67 -0.6
211,172 + GFF899 0.70 -0.5
211,173 - GFF899 0.70 -0.9
211,173 - GFF899 0.70 -0.4
211,193 + GFF899 0.71 +1.0
211,193 + GFF899 0.71 -0.8
211,194 - GFF899 0.71 -0.7
211,194 - GFF899 0.71 -0.0
211,194 - GFF899 0.71 -0.5
211,194 - GFF899 0.71 -2.3
211,195 + GFF899 0.71 -0.9
211,195 + GFF899 0.71 -0.5
211,196 - GFF899 0.72 -0.5
211,297 + GFF899 0.80 +2.0
211,298 - GFF899 0.80 -0.5

Or see this region's nucleotide sequence