Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF839 and GFF840 overlap by 4 nucleotides GFF840 and GFF841 are separated by 11 nucleotides GFF841 and GFF842 overlap by 4 nucleotides
GFF839 - Retron-type RNA-directed DNA polymerase (EC 2.7.7.49), at 144,870 to 146,816
GFF839
GFF840 - no description, at 146,813 to 147,082
GFF840
GFF841 - zinc finger, SWIM-type, at 147,094 to 148,800
GFF841
GFF842 - hypothetical protein, at 148,797 to 151,982
GFF842
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-1
0
1 at 146.176 kb on + strand, within GFF839 at 146.435 kb on - strand, within GFF839 at 146.435 kb on - strand, within GFF839 at 146.919 kb on - strand, within GFF840 at 146.981 kb on + strand, within GFF840 at 147.092 kb on + strand at 147.092 kb on + strand at 147.092 kb on + strand at 147.503 kb on + strand, within GFF841 at 147.504 kb on - strand, within GFF841 at 147.504 kb on - strand, within GFF841 at 147.527 kb on + strand, within GFF841 at 148.199 kb on + strand, within GFF841 at 148.199 kb on + strand, within GFF841 at 148.317 kb on - strand, within GFF841 at 148.317 kb on - strand, within GFF841 at 148.415 kb on + strand, within GFF841 at 148.415 kb on + strand, within GFF841 at 148.415 kb on + strand, within GFF841 at 148.416 kb on - strand, within GFF841 at 148.416 kb on - strand, within GFF841 at 148.416 kb on - strand, within GFF841 at 148.416 kb on - strand, within GFF841 at 148.592 kb on + strand, within GFF841 at 148.593 kb on - strand, within GFF841 at 148.788 kb on - strand at 148.927 kb on + strand at 148.927 kb on + strand at 148.927 kb on + strand at 148.927 kb on + strand at 148.928 kb on - strand at 149.092 kb on + strand at 149.093 kb on - strand at 149.203 kb on + strand, within GFF842 at 149.203 kb on + strand, within GFF842 at 149.203 kb on + strand, within GFF842 at 149.203 kb on + strand, within GFF842 at 149.204 kb on - strand, within GFF842
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 146,176 + GFF839 0.67 -0.8 146,435 - GFF839 0.80 -0.5 146,435 - GFF839 0.80 -0.1 146,919 - GFF840 0.39 -0.5 146,981 + GFF840 0.62 -0.5 147,092 + +0.2 147,092 + -0.7 147,092 + -0.4 147,503 + GFF841 0.24 +0.3 147,504 - GFF841 0.24 -0.4 147,504 - GFF841 0.24 -0.1 147,527 + GFF841 0.25 +0.4 148,199 + GFF841 0.65 -0.5 148,199 + GFF841 0.65 -0.2 148,317 - GFF841 0.72 +0.4 148,317 - GFF841 0.72 -0.4 148,415 + GFF841 0.77 +0.8 148,415 + GFF841 0.77 +1.7 148,415 + GFF841 0.77 +0.5 148,416 - GFF841 0.77 -0.1 148,416 - GFF841 0.77 -0.1 148,416 - GFF841 0.77 -1.3 148,416 - GFF841 0.77 -1.1 148,592 + GFF841 0.88 -0.6 148,593 - GFF841 0.88 -0.4 148,788 - +0.6 148,927 + +0.4 148,927 + -0.7 148,927 + -0.8 148,927 + +0.9 148,928 - +0.1 149,092 + +0.4 149,093 - +0.0 149,203 + GFF842 0.13 -0.5 149,203 + GFF842 0.13 +0.2 149,203 + GFF842 0.13 -0.5 149,203 + GFF842 0.13 -0.6 149,204 - GFF842 0.13 +0.4
Or see this region's nucleotide sequence