Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF781 and GFF782 are separated by 5 nucleotides GFF782 and GFF783 are separated by 101 nucleotides GFF783 and GFF784 are separated by 11 nucleotides GFF784 and GFF785 are separated by 5 nucleotides
GFF781 - hypothetical protein, at 62,685 to 64,532
GFF781
GFF782 - Alpha-ketoglutarate-dependent dioxygenase AlkB (EC 1.14.11.33), at 64,538 to 65,245
GFF782
GFF783 - no description, at 65,347 to 66,069
GFF783
GFF784 - KEGG: RNA polymerase sigma-70 factor, ECF subfamily, at 66,081 to 66,641
GFF784
GFF785 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 66,647 to 67,636
GFF785
Position (kb) 65
66
67 Strain fitness (log2 ratio) -2
-1
0
1
2 at 64.465 kb on + strand at 64.465 kb on + strand at 64.466 kb on - strand at 64.537 kb on - strand at 64.682 kb on + strand, within GFF782 at 64.683 kb on - strand, within GFF782 at 64.764 kb on + strand, within GFF782 at 64.765 kb on - strand, within GFF782 at 64.815 kb on + strand, within GFF782 at 64.816 kb on - strand, within GFF782 at 65.268 kb on + strand at 65.268 kb on + strand at 65.268 kb on + strand at 65.289 kb on + strand at 65.289 kb on + strand at 65.292 kb on + strand at 65.292 kb on + strand at 65.293 kb on - strand at 65.293 kb on - strand at 65.656 kb on + strand, within GFF783 at 66.132 kb on + strand at 66.132 kb on + strand at 66.132 kb on + strand at 66.132 kb on + strand at 66.133 kb on - strand at 66.261 kb on + strand, within GFF784 at 66.261 kb on + strand, within GFF784 at 66.631 kb on + strand at 66.631 kb on + strand at 66.631 kb on + strand at 66.632 kb on - strand at 66.634 kb on + strand at 66.789 kb on + strand, within GFF785 at 66.790 kb on - strand, within GFF785 at 66.917 kb on + strand, within GFF785 at 67.017 kb on - strand, within GFF785
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 64,465 + +0.6 64,465 + -1.0 64,466 - +0.2 64,537 - +0.2 64,682 + GFF782 0.20 -0.2 64,683 - GFF782 0.20 -1.1 64,764 + GFF782 0.32 +0.3 64,765 - GFF782 0.32 -0.1 64,815 + GFF782 0.39 +2.0 64,816 - GFF782 0.39 +0.2 65,268 + -0.8 65,268 + +0.2 65,268 + -0.7 65,289 + +0.9 65,289 + -0.1 65,292 + -0.1 65,292 + +1.4 65,293 - -0.8 65,293 - -0.6 65,656 + GFF783 0.43 -0.1 66,132 + -1.5 66,132 + -0.1 66,132 + -1.7 66,132 + -0.5 66,133 - -0.1 66,261 + GFF784 0.32 +0.6 66,261 + GFF784 0.32 -1.1 66,631 + +1.3 66,631 + -0.5 66,631 + +2.4 66,632 - -0.8 66,634 + -0.7 66,789 + GFF785 0.14 -0.2 66,790 - GFF785 0.14 -1.5 66,917 + GFF785 0.27 +0.1 67,017 - GFF785 0.37 -1.8
Or see this region's nucleotide sequence