Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7366 and GFF7367 are separated by 425 nucleotides GFF7367 and GFF7368 overlap by 37 nucleotides GFF7368 and GFF7369 are separated by 63 nucleotides
GFF7366 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 13,023 to 14,591
GFF7366
GFF7367 - Enoyl-CoA hydratase (EC 4.2.1.17), at 15,017 to 15,208
GFF7367
GFF7368 - Enoyl-CoA hydratase (EC 4.2.1.17), at 15,172 to 15,474
GFF7368
GFF7369 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 15,538 to 16,722
GFF7369
Position (kb)
15
16 Strain fitness (log2 ratio)
-1
0
1 at 14.093 kb on + strand, within GFF7366 at 14.108 kb on - strand, within GFF7366 at 14.182 kb on + strand, within GFF7366 at 14.335 kb on + strand, within GFF7366 at 14.335 kb on + strand, within GFF7366 at 14.524 kb on + strand at 14.524 kb on + strand at 14.524 kb on + strand at 14.524 kb on + strand at 14.525 kb on - strand at 14.883 kb on - strand at 14.883 kb on - strand at 14.890 kb on - strand at 15.152 kb on + strand, within GFF7367 at 15.152 kb on + strand, within GFF7367 at 15.152 kb on + strand, within GFF7367 at 15.152 kb on + strand, within GFF7367 at 15.153 kb on - strand, within GFF7367 at 15.153 kb on - strand, within GFF7367 at 15.153 kb on - strand, within GFF7367 at 15.153 kb on - strand, within GFF7367 at 15.364 kb on + strand, within GFF7368 at 15.533 kb on + strand at 15.706 kb on + strand, within GFF7369 at 15.706 kb on + strand, within GFF7369 at 15.706 kb on + strand, within GFF7369 at 15.706 kb on + strand, within GFF7369 at 15.706 kb on + strand, within GFF7369 at 15.706 kb on + strand, within GFF7369 at 15.707 kb on - strand, within GFF7369 at 15.884 kb on + strand, within GFF7369 at 15.884 kb on + strand, within GFF7369 at 15.885 kb on - strand, within GFF7369 at 15.885 kb on - strand, within GFF7369 at 15.885 kb on - strand, within GFF7369 at 15.885 kb on - strand, within GFF7369 at 15.885 kb on - strand, within GFF7369 at 15.889 kb on + strand, within GFF7369 at 15.889 kb on + strand, within GFF7369 at 15.889 kb on + strand, within GFF7369 at 15.890 kb on - strand, within GFF7369 at 15.890 kb on - strand, within GFF7369 at 15.890 kb on - strand, within GFF7369 at 15.890 kb on - strand, within GFF7369 at 15.890 kb on - strand, within GFF7369 at 15.890 kb on - strand, within GFF7369 at 15.890 kb on - strand, within GFF7369 at 16.051 kb on - strand, within GFF7369 at 16.051 kb on - strand, within GFF7369 at 16.195 kb on + strand, within GFF7369 at 16.195 kb on + strand, within GFF7369 at 16.195 kb on + strand, within GFF7369
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 14,093 + GFF7366 0.68 +0.8 14,108 - GFF7366 0.69 -0.3 14,182 + GFF7366 0.74 -0.5 14,335 + GFF7366 0.84 +0.4 14,335 + GFF7366 0.84 -1.0 14,524 + -1.2 14,524 + -0.1 14,524 + +1.0 14,524 + +0.5 14,525 - +0.2 14,883 - +0.4 14,883 - -0.1 14,890 - +1.4 15,152 + GFF7367 0.70 -0.5 15,152 + GFF7367 0.70 -0.7 15,152 + GFF7367 0.70 -0.4 15,152 + GFF7367 0.70 +0.0 15,153 - GFF7367 0.71 +0.2 15,153 - GFF7367 0.71 +0.3 15,153 - GFF7367 0.71 -0.4 15,153 - GFF7367 0.71 -0.0 15,364 + GFF7368 0.63 -0.1 15,533 + -0.5 15,706 + GFF7369 0.14 -1.0 15,706 + GFF7369 0.14 +0.1 15,706 + GFF7369 0.14 -0.4 15,706 + GFF7369 0.14 -0.7 15,706 + GFF7369 0.14 +0.0 15,706 + GFF7369 0.14 +1.4 15,707 - GFF7369 0.14 -0.1 15,884 + GFF7369 0.29 -0.6 15,884 + GFF7369 0.29 +0.8 15,885 - GFF7369 0.29 -0.4 15,885 - GFF7369 0.29 -0.2 15,885 - GFF7369 0.29 -1.0 15,885 - GFF7369 0.29 +0.5 15,885 - GFF7369 0.29 -0.4 15,889 + GFF7369 0.30 -1.3 15,889 + GFF7369 0.30 +0.1 15,889 + GFF7369 0.30 -0.2 15,890 - GFF7369 0.30 -0.6 15,890 - GFF7369 0.30 +0.5 15,890 - GFF7369 0.30 -0.5 15,890 - GFF7369 0.30 +0.2 15,890 - GFF7369 0.30 -0.4 15,890 - GFF7369 0.30 -0.8 15,890 - GFF7369 0.30 -0.3 16,051 - GFF7369 0.43 -0.5 16,051 - GFF7369 0.43 -0.9 16,195 + GFF7369 0.55 +1.1 16,195 + GFF7369 0.55 -0.2 16,195 + GFF7369 0.55 -1.1
Or see this region's nucleotide sequence