Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7216 and GFF7217 are separated by 38 nucleotides GFF7217 and GFF7218 are separated by 40 nucleotides GFF7218 and GFF7219 are separated by 555 nucleotides
GFF7216 - hypothetical protein, at 35,085 to 35,549
GFF7216
GFF7217 - hypothetical protein, at 35,588 to 36,508
GFF7217
GFF7218 - no description, at 36,549 to 37,253
GFF7218
GFF7219 - no description, at 37,809 to 37,898
GFF7219
Position (kb) 36
37
38 Strain fitness (log2 ratio) -2
-1
0
1 at 35.844 kb on - strand, within GFF7217 at 36.000 kb on + strand, within GFF7217 at 36.057 kb on - strand, within GFF7217 at 36.284 kb on + strand, within GFF7217 at 36.285 kb on - strand, within GFF7217 at 36.562 kb on + strand at 36.562 kb on + strand at 36.562 kb on + strand at 36.563 kb on - strand at 36.563 kb on - strand at 36.714 kb on + strand, within GFF7218 at 36.714 kb on + strand, within GFF7218 at 36.714 kb on + strand, within GFF7218 at 36.715 kb on - strand, within GFF7218 at 36.715 kb on - strand, within GFF7218 at 36.760 kb on + strand, within GFF7218 at 36.962 kb on - strand, within GFF7218 at 36.991 kb on + strand, within GFF7218 at 36.991 kb on + strand, within GFF7218 at 37.038 kb on - strand, within GFF7218 at 37.054 kb on + strand, within GFF7218 at 37.054 kb on + strand, within GFF7218 at 37.054 kb on + strand, within GFF7218 at 37.406 kb on + strand at 37.414 kb on - strand at 37.578 kb on + strand at 37.636 kb on - strand at 37.757 kb on + strand at 37.814 kb on + strand at 37.815 kb on - strand at 37.815 kb on - strand at 37.815 kb on - strand at 37.815 kb on - strand at 37.825 kb on - strand, within GFF7219
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 35,844 - GFF7217 0.28 -0.5 36,000 + GFF7217 0.45 -1.0 36,057 - GFF7217 0.51 -0.9 36,284 + GFF7217 0.76 +0.8 36,285 - GFF7217 0.76 +0.2 36,562 + -0.9 36,562 + -0.9 36,562 + +0.6 36,563 - +1.0 36,563 - +0.3 36,714 + GFF7218 0.23 -0.3 36,714 + GFF7218 0.23 -0.3 36,714 + GFF7218 0.23 +0.0 36,715 - GFF7218 0.24 -1.1 36,715 - GFF7218 0.24 -0.2 36,760 + GFF7218 0.30 +0.9 36,962 - GFF7218 0.59 +0.3 36,991 + GFF7218 0.63 +0.1 36,991 + GFF7218 0.63 -0.1 37,038 - GFF7218 0.69 +0.3 37,054 + GFF7218 0.72 +0.4 37,054 + GFF7218 0.72 -0.1 37,054 + GFF7218 0.72 +0.8 37,406 + -1.1 37,414 - -1.1 37,578 + -0.3 37,636 - -1.1 37,757 + +0.2 37,814 + -0.0 37,815 - -0.4 37,815 - -1.8 37,815 - -0.1 37,815 - -0.1 37,825 - GFF7219 0.18 +1.0
Or see this region's nucleotide sequence