Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7213 and GFF7214 are separated by 72 nucleotides GFF7214 and GFF7215 are separated by 170 nucleotides
GFF7213 - DNA polymerase II (EC 2.7.7.7), at 30,183 to 32,597
GFF7213
GFF7214 - Peptidase M20D, amidohydrolase, at 32,670 to 33,980
GFF7214
GFF7215 - Transcriptional regulator, LysR family, at 34,151 to 35,035
GFF7215
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-1
0
1
2 at 31.846 kb on - strand, within GFF7213 at 31.846 kb on - strand, within GFF7213 at 32.048 kb on - strand, within GFF7213 at 32.048 kb on - strand, within GFF7213 at 32.290 kb on + strand, within GFF7213 at 32.866 kb on + strand, within GFF7214 at 32.866 kb on + strand, within GFF7214 at 32.908 kb on - strand, within GFF7214 at 32.951 kb on + strand, within GFF7214 at 33.098 kb on - strand, within GFF7214 at 33.635 kb on - strand, within GFF7214 at 33.695 kb on + strand, within GFF7214 at 33.696 kb on - strand, within GFF7214 at 33.847 kb on + strand, within GFF7214 at 33.848 kb on - strand, within GFF7214 at 33.868 kb on + strand at 33.869 kb on - strand at 34.068 kb on + strand at 34.161 kb on + strand at 34.162 kb on - strand at 34.162 kb on - strand at 34.701 kb on + strand, within GFF7215 at 34.781 kb on + strand, within GFF7215 at 34.781 kb on + strand, within GFF7215 at 34.781 kb on + strand, within GFF7215 at 34.782 kb on - strand, within GFF7215 at 34.782 kb on - strand, within GFF7215 at 34.782 kb on - strand, within GFF7215 at 34.932 kb on + strand, within GFF7215 at 34.933 kb on - strand, within GFF7215 at 34.933 kb on - strand, within GFF7215 at 34.933 kb on - strand, within GFF7215 at 34.933 kb on - strand, within GFF7215
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 31,846 - GFF7213 0.69 +0.3 31,846 - GFF7213 0.69 -0.4 32,048 - GFF7213 0.77 -0.8 32,048 - GFF7213 0.77 -0.3 32,290 + GFF7213 0.87 +0.7 32,866 + GFF7214 0.15 -0.7 32,866 + GFF7214 0.15 +0.8 32,908 - GFF7214 0.18 +1.1 32,951 + GFF7214 0.21 +0.8 33,098 - GFF7214 0.33 +0.1 33,635 - GFF7214 0.74 +0.2 33,695 + GFF7214 0.78 -1.5 33,696 - GFF7214 0.78 +0.3 33,847 + GFF7214 0.90 -1.3 33,848 - GFF7214 0.90 +0.2 33,868 + +0.1 33,869 - +1.9 34,068 + -0.0 34,161 + -0.7 34,162 - +1.1 34,162 - -1.0 34,701 + GFF7215 0.62 -0.4 34,781 + GFF7215 0.71 +0.3 34,781 + GFF7215 0.71 +0.5 34,781 + GFF7215 0.71 -1.5 34,782 - GFF7215 0.71 -1.1 34,782 - GFF7215 0.71 -0.9 34,782 - GFF7215 0.71 -0.2 34,932 + GFF7215 0.88 -0.1 34,933 - GFF7215 0.88 -0.3 34,933 - GFF7215 0.88 +0.1 34,933 - GFF7215 0.88 -1.6 34,933 - GFF7215 0.88 +0.3
Or see this region's nucleotide sequence