Strain Fitness in Variovorax sp. SCN45 around GFF7214

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7213 and GFF7214 are separated by 72 nucleotidesGFF7214 and GFF7215 are separated by 170 nucleotides GFF7213 - DNA polymerase II (EC 2.7.7.7), at 30,183 to 32,597 GFF7213 GFF7214 - Peptidase M20D, amidohydrolase, at 32,670 to 33,980 GFF7214 GFF7215 - Transcriptional regulator, LysR family, at 34,151 to 35,035 GFF7215 Position (kb) 32 33 34Strain fitness (log2 ratio) -1 0 1 2at 31.846 kb on - strand, within GFF7213at 31.846 kb on - strand, within GFF7213at 32.048 kb on - strand, within GFF7213at 32.048 kb on - strand, within GFF7213at 32.290 kb on + strand, within GFF7213at 32.866 kb on + strand, within GFF7214at 32.866 kb on + strand, within GFF7214at 32.908 kb on - strand, within GFF7214at 32.951 kb on + strand, within GFF7214at 33.098 kb on - strand, within GFF7214at 33.635 kb on - strand, within GFF7214at 33.695 kb on + strand, within GFF7214at 33.696 kb on - strand, within GFF7214at 33.847 kb on + strand, within GFF7214at 33.848 kb on - strand, within GFF7214at 33.868 kb on + strandat 33.869 kb on - strandat 34.068 kb on + strandat 34.161 kb on + strandat 34.162 kb on - strandat 34.162 kb on - strandat 34.701 kb on + strand, within GFF7215at 34.781 kb on + strand, within GFF7215at 34.781 kb on + strand, within GFF7215at 34.781 kb on + strand, within GFF7215at 34.782 kb on - strand, within GFF7215at 34.782 kb on - strand, within GFF7215at 34.782 kb on - strand, within GFF7215at 34.932 kb on + strand, within GFF7215at 34.933 kb on - strand, within GFF7215at 34.933 kb on - strand, within GFF7215at 34.933 kb on - strand, within GFF7215at 34.933 kb on - strand, within GFF7215

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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31,846 - GFF7213 0.69 +0.3
31,846 - GFF7213 0.69 -0.4
32,048 - GFF7213 0.77 -0.8
32,048 - GFF7213 0.77 -0.3
32,290 + GFF7213 0.87 +0.7
32,866 + GFF7214 0.15 -0.7
32,866 + GFF7214 0.15 +0.8
32,908 - GFF7214 0.18 +1.1
32,951 + GFF7214 0.21 +0.8
33,098 - GFF7214 0.33 +0.1
33,635 - GFF7214 0.74 +0.2
33,695 + GFF7214 0.78 -1.5
33,696 - GFF7214 0.78 +0.3
33,847 + GFF7214 0.90 -1.3
33,848 - GFF7214 0.90 +0.2
33,868 + +0.1
33,869 - +1.9
34,068 + -0.0
34,161 + -0.7
34,162 - +1.1
34,162 - -1.0
34,701 + GFF7215 0.62 -0.4
34,781 + GFF7215 0.71 +0.3
34,781 + GFF7215 0.71 +0.5
34,781 + GFF7215 0.71 -1.5
34,782 - GFF7215 0.71 -1.1
34,782 - GFF7215 0.71 -0.9
34,782 - GFF7215 0.71 -0.2
34,932 + GFF7215 0.88 -0.1
34,933 - GFF7215 0.88 -0.3
34,933 - GFF7215 0.88 +0.1
34,933 - GFF7215 0.88 -1.6
34,933 - GFF7215 0.88 +0.3

Or see this region's nucleotide sequence