Strain Fitness in Variovorax sp. SCN45 around GFF7133

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7132 and GFF7133 are separated by 5 nucleotidesGFF7133 and GFF7134 are separated by 85 nucleotides GFF7132 - Xylose isomerase-like TIM-barrel protein KPN_00539, at 3 to 761 GFF7132 GFF7133 - Catalase-like heme-binding protein, at 767 to 1,852 GFF7133 GFF7134 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 1,938 to 3,395 GFF7134 Position (kb) 0 1 2Strain fitness (log2 ratio) -2 -1 0 1 2at 0.047 kb on + strandat 0.047 kb on + strandat 0.047 kb on + strandat 0.047 kb on + strandat 0.055 kb on + strandat 0.141 kb on + strand, within GFF7132at 0.141 kb on + strand, within GFF7132at 0.142 kb on - strand, within GFF7132at 0.142 kb on - strand, within GFF7132at 0.142 kb on - strand, within GFF7132at 0.142 kb on - strand, within GFF7132at 0.147 kb on + strand, within GFF7132at 0.147 kb on + strand, within GFF7132at 0.147 kb on + strand, within GFF7132at 0.147 kb on + strand, within GFF7132at 0.147 kb on + strand, within GFF7132at 0.147 kb on + strand, within GFF7132at 0.147 kb on + strand, within GFF7132at 0.147 kb on + strand, within GFF7132at 0.148 kb on - strand, within GFF7132at 0.148 kb on - strand, within GFF7132at 0.148 kb on - strand, within GFF7132at 0.259 kb on - strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.300 kb on + strand, within GFF7132at 0.301 kb on - strand, within GFF7132at 0.301 kb on - strand, within GFF7132at 0.301 kb on - strand, within GFF7132at 0.301 kb on - strand, within GFF7132at 0.301 kb on - strand, within GFF7132at 0.301 kb on - strand, within GFF7132at 0.301 kb on - strand, within GFF7132at 0.301 kb on - strand, within GFF7132at 0.351 kb on + strand, within GFF7132at 0.351 kb on + strand, within GFF7132at 0.352 kb on - strand, within GFF7132at 0.352 kb on - strand, within GFF7132at 0.352 kb on - strand, within GFF7132at 0.387 kb on + strand, within GFF7132at 0.387 kb on + strand, within GFF7132at 0.388 kb on - strand, within GFF7132at 0.388 kb on - strand, within GFF7132at 0.388 kb on - strand, within GFF7132at 0.388 kb on - strand, within GFF7132at 0.388 kb on - strand, within GFF7132at 0.396 kb on + strand, within GFF7132at 0.396 kb on + strand, within GFF7132at 0.396 kb on + strand, within GFF7132at 0.396 kb on + strand, within GFF7132at 0.396 kb on + strand, within GFF7132at 0.396 kb on + strand, within GFF7132at 0.396 kb on + strand, within GFF7132at 0.397 kb on - strand, within GFF7132at 0.397 kb on - strand, within GFF7132at 0.397 kb on - strand, within GFF7132at 0.397 kb on - strand, within GFF7132at 0.397 kb on - strand, within GFF7132at 0.397 kb on - strand, within GFF7132at 0.408 kb on + strand, within GFF7132at 0.408 kb on + strand, within GFF7132at 0.408 kb on + strand, within GFF7132at 0.408 kb on + strand, within GFF7132at 0.409 kb on - strand, within GFF7132at 0.409 kb on - strand, within GFF7132at 0.409 kb on - strand, within GFF7132at 0.409 kb on - strand, within GFF7132at 0.477 kb on + strand, within GFF7132at 0.477 kb on + strand, within GFF7132at 0.478 kb on - strand, within GFF7132at 0.478 kb on - strand, within GFF7132at 0.478 kb on - strand, within GFF7132at 0.478 kb on - strand, within GFF7132at 0.576 kb on + strand, within GFF7132at 0.576 kb on + strand, within GFF7132at 0.576 kb on + strand, within GFF7132at 0.577 kb on - strand, within GFF7132at 0.577 kb on - strand, within GFF7132at 0.577 kb on - strand, within GFF7132at 0.804 kb on + strandat 0.805 kb on - strandat 0.822 kb on + strandat 0.822 kb on + strandat 0.822 kb on + strandat 0.822 kb on + strandat 0.822 kb on + strandat 0.822 kb on + strandat 0.822 kb on + strandat 0.822 kb on + strandat 0.822 kb on + strandat 0.823 kb on - strandat 0.823 kb on - strandat 0.823 kb on - strandat 0.823 kb on - strandat 1.197 kb on + strand, within GFF7133at 1.197 kb on + strand, within GFF7133at 1.198 kb on - strand, within GFF7133at 1.198 kb on - strand, within GFF7133at 1.198 kb on - strand, within GFF7133at 1.198 kb on - strand, within GFF7133at 1.198 kb on - strand, within GFF7133at 1.198 kb on - strand, within GFF7133at 1.198 kb on - strand, within GFF7133at 1.198 kb on - strand, within GFF7133at 1.206 kb on + strand, within GFF7133at 1.888 kb on + strandat 1.888 kb on + strandat 1.888 kb on + strandat 1.889 kb on - strandat 1.889 kb on - strandat 1.889 kb on - strandat 1.919 kb on + strandat 1.919 kb on + strandat 1.919 kb on + strandat 1.919 kb on + strandat 1.919 kb on + strandat 1.919 kb on + strandat 1.919 kb on + strandat 1.919 kb on + strandat 1.920 kb on - strandat 1.920 kb on - strandat 1.920 kb on - strandat 1.920 kb on - strandat 1.920 kb on - strandat 2.230 kb on + strand, within GFF7134at 2.230 kb on + strand, within GFF7134at 2.230 kb on + strand, within GFF7134at 2.231 kb on - strand, within GFF7134at 2.231 kb on - strand, within GFF7134at 2.231 kb on - strand, within GFF7134at 2.231 kb on - strand, within GFF7134at 2.231 kb on - strand, within GFF7134at 2.343 kb on + strand, within GFF7134at 2.343 kb on + strand, within GFF7134at 2.343 kb on + strand, within GFF7134at 2.343 kb on + strand, within GFF7134at 2.344 kb on - strand, within GFF7134at 2.344 kb on - strand, within GFF7134at 2.344 kb on - strand, within GFF7134at 2.428 kb on + strand, within GFF7134at 2.428 kb on + strand, within GFF7134at 2.428 kb on + strand, within GFF7134at 2.428 kb on + strand, within GFF7134at 2.429 kb on - strand, within GFF7134at 2.429 kb on - strand, within GFF7134at 2.429 kb on - strand, within GFF7134at 2.429 kb on - strand, within GFF7134at 2.429 kb on - strand, within GFF7134at 2.429 kb on - strand, within GFF7134at 2.429 kb on - strand, within GFF7134at 2.503 kb on + strand, within GFF7134at 2.503 kb on + strand, within GFF7134at 2.503 kb on + strand, within GFF7134at 2.503 kb on + strand, within GFF7134at 2.504 kb on - strand, within GFF7134at 2.504 kb on - strand, within GFF7134at 2.504 kb on - strand, within GFF7134at 2.504 kb on - strand, within GFF7134at 2.504 kb on - strand, within GFF7134at 2.504 kb on - strand, within GFF7134at 2.504 kb on - strand, within GFF7134at 2.504 kb on - strand, within GFF7134at 2.725 kb on + strand, within GFF7134at 2.725 kb on + strand, within GFF7134at 2.725 kb on + strand, within GFF7134at 2.725 kb on + strand, within GFF7134at 2.725 kb on + strand, within GFF7134at 2.725 kb on + strand, within GFF7134at 2.726 kb on - strand, within GFF7134at 2.726 kb on - strand, within GFF7134at 2.726 kb on - strand, within GFF7134at 2.726 kb on - strand, within GFF7134

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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47 + -0.4
47 + +0.4
47 + -0.0
47 + -0.4
55 + +0.1
141 + GFF7132 0.18 -0.3
141 + GFF7132 0.18 +0.2
142 - GFF7132 0.18 +0.5
142 - GFF7132 0.18 +0.8
142 - GFF7132 0.18 +1.4
142 - GFF7132 0.18 +1.1
147 + GFF7132 0.19 +1.3
147 + GFF7132 0.19 -0.5
147 + GFF7132 0.19 +0.7
147 + GFF7132 0.19 -0.6
147 + GFF7132 0.19 -0.2
147 + GFF7132 0.19 -2.1
147 + GFF7132 0.19 +0.3
147 + GFF7132 0.19 -0.4
148 - GFF7132 0.19 +0.4
148 - GFF7132 0.19 +0.2
148 - GFF7132 0.19 -0.8
259 - GFF7132 0.34 -0.7
300 + GFF7132 0.39 -0.7
300 + GFF7132 0.39 +1.3
300 + GFF7132 0.39 +0.0
300 + GFF7132 0.39 +0.4
300 + GFF7132 0.39 +0.3
300 + GFF7132 0.39 +0.5
300 + GFF7132 0.39 -0.8
300 + GFF7132 0.39 +0.0
300 + GFF7132 0.39 -1.1
300 + GFF7132 0.39 +0.3
300 + GFF7132 0.39 +0.1
301 - GFF7132 0.39 -1.9
301 - GFF7132 0.39 -0.4
301 - GFF7132 0.39 +1.6
301 - GFF7132 0.39 -0.9
301 - GFF7132 0.39 -1.7
301 - GFF7132 0.39 -0.1
301 - GFF7132 0.39 -0.8
301 - GFF7132 0.39 -0.7
351 + GFF7132 0.46 -0.2
351 + GFF7132 0.46 -0.9
352 - GFF7132 0.46 +0.3
352 - GFF7132 0.46 -0.6
352 - GFF7132 0.46 -0.3
387 + GFF7132 0.51 -0.0
387 + GFF7132 0.51 +1.0
388 - GFF7132 0.51 +0.1
388 - GFF7132 0.51 -0.4
388 - GFF7132 0.51 +1.3
388 - GFF7132 0.51 -0.2
388 - GFF7132 0.51 +0.3
396 + GFF7132 0.52 -0.4
396 + GFF7132 0.52 +1.4
396 + GFF7132 0.52 +0.1
396 + GFF7132 0.52 -0.0
396 + GFF7132 0.52 -0.2
396 + GFF7132 0.52 -0.0
396 + GFF7132 0.52 -1.3
397 - GFF7132 0.52 -1.7
397 - GFF7132 0.52 -0.7
397 - GFF7132 0.52 +0.7
397 - GFF7132 0.52 +0.0
397 - GFF7132 0.52 +0.1
397 - GFF7132 0.52 +0.3
408 + GFF7132 0.53 +1.9
408 + GFF7132 0.53 -0.6
408 + GFF7132 0.53 -0.1
408 + GFF7132 0.53 +0.5
409 - GFF7132 0.53 +0.2
409 - GFF7132 0.53 +0.1
409 - GFF7132 0.53 -1.2
409 - GFF7132 0.53 +1.4
477 + GFF7132 0.62 +0.4
477 + GFF7132 0.62 -2.4
478 - GFF7132 0.63 -0.5
478 - GFF7132 0.63 -1.0
478 - GFF7132 0.63 -0.6
478 - GFF7132 0.63 -0.6
576 + GFF7132 0.75 +0.7
576 + GFF7132 0.75 +0.2
576 + GFF7132 0.75 -0.9
577 - GFF7132 0.76 +0.4
577 - GFF7132 0.76 +0.3
577 - GFF7132 0.76 +0.4
804 + +0.1
805 - -0.6
822 + -0.3
822 + +0.9
822 + +1.4
822 + -0.5
822 + +0.1
822 + -0.8
822 + +0.1
822 + +0.1
822 + -0.8
823 - -0.1
823 - -0.2
823 - -1.0
823 - -0.1
1,197 + GFF7133 0.40 -0.3
1,197 + GFF7133 0.40 -0.4
1,198 - GFF7133 0.40 +0.7
1,198 - GFF7133 0.40 -0.4
1,198 - GFF7133 0.40 -1.0
1,198 - GFF7133 0.40 -0.7
1,198 - GFF7133 0.40 +0.1
1,198 - GFF7133 0.40 +1.9
1,198 - GFF7133 0.40 -1.6
1,198 - GFF7133 0.40 +0.5
1,206 + GFF7133 0.40 -0.3
1,888 + +0.1
1,888 + -0.7
1,888 + +0.1
1,889 - +0.0
1,889 - -0.1
1,889 - +0.6
1,919 + +0.5
1,919 + -1.1
1,919 + -1.6
1,919 + +0.4
1,919 + -0.2
1,919 + -0.8
1,919 + +0.2
1,919 + -1.2
1,920 - +0.0
1,920 - +0.0
1,920 - -0.1
1,920 - -0.5
1,920 - +0.9
2,230 + GFF7134 0.20 +1.4
2,230 + GFF7134 0.20 -1.5
2,230 + GFF7134 0.20 -0.4
2,231 - GFF7134 0.20 -0.1
2,231 - GFF7134 0.20 -0.6
2,231 - GFF7134 0.20 +0.6
2,231 - GFF7134 0.20 -0.1
2,231 - GFF7134 0.20 -0.5
2,343 + GFF7134 0.28 -0.1
2,343 + GFF7134 0.28 -0.0
2,343 + GFF7134 0.28 -1.4
2,343 + GFF7134 0.28 -0.1
2,344 - GFF7134 0.28 -1.2
2,344 - GFF7134 0.28 -0.6
2,344 - GFF7134 0.28 -0.6
2,428 + GFF7134 0.34 -0.3
2,428 + GFF7134 0.34 +1.0
2,428 + GFF7134 0.34 -1.1
2,428 + GFF7134 0.34 -0.9
2,429 - GFF7134 0.34 -0.7
2,429 - GFF7134 0.34 +0.2
2,429 - GFF7134 0.34 +0.1
2,429 - GFF7134 0.34 +0.0
2,429 - GFF7134 0.34 +0.3
2,429 - GFF7134 0.34 -0.6
2,429 - GFF7134 0.34 -0.2
2,503 + GFF7134 0.39 -1.2
2,503 + GFF7134 0.39 -0.1
2,503 + GFF7134 0.39 +0.5
2,503 + GFF7134 0.39 -1.0
2,504 - GFF7134 0.39 -0.3
2,504 - GFF7134 0.39 -0.1
2,504 - GFF7134 0.39 -0.7
2,504 - GFF7134 0.39 -0.2
2,504 - GFF7134 0.39 -0.5
2,504 - GFF7134 0.39 -0.4
2,504 - GFF7134 0.39 +0.2
2,504 - GFF7134 0.39 -1.1
2,725 + GFF7134 0.54 -0.3
2,725 + GFF7134 0.54 -0.5
2,725 + GFF7134 0.54 +0.8
2,725 + GFF7134 0.54 -0.4
2,725 + GFF7134 0.54 -0.8
2,725 + GFF7134 0.54 +0.1
2,726 - GFF7134 0.54 -0.5
2,726 - GFF7134 0.54 +0.0
2,726 - GFF7134 0.54 +0.0
2,726 - GFF7134 0.54 +0.0

Or see this region's nucleotide sequence