Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7116 and GFF7117 overlap by 4 nucleotides GFF7117 and GFF7118 overlap by 4 nucleotides GFF7118 and GFF7119 are separated by 6 nucleotides GFF7119 and GFF7120 are separated by 1 nucleotides
GFF7116 - 2,3-dihydroxybiphenyl 1,2-dioxygenase (EC 1.13.11.39), at 34,498 to 35,412
GFF7116
GFF7117 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 35,409 to 36,239
GFF7117
GFF7118 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 36,236 to 37,177
GFF7118
GFF7119 - Ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA, at 37,184 to 37,660
GFF7119
GFF7120 - Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB, at 37,662 to 38,906
GFF7120
Position (kb) 36
37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 35.315 kb on + strand, within GFF7116 at 35.315 kb on + strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.357 kb on + strand at 35.357 kb on + strand at 35.358 kb on - strand at 35.582 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.797 kb on + strand, within GFF7117 at 35.971 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.993 kb on - strand, within GFF7117 at 35.993 kb on - strand, within GFF7117 at 36.004 kb on + strand, within GFF7117 at 36.094 kb on + strand, within GFF7117 at 36.094 kb on + strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.109 kb on + strand, within GFF7117 at 36.175 kb on + strand at 36.175 kb on + strand at 36.175 kb on + strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.187 kb on - strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.921 kb on - strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 37.032 kb on + strand, within GFF7118 at 37.185 kb on + strand at 37.185 kb on + strand at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.291 kb on - strand, within GFF7119 at 37.291 kb on - strand, within GFF7119 at 37.291 kb on - strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.545 kb on + strand, within GFF7119 at 37.546 kb on - strand, within GFF7119 at 37.546 kb on - strand, within GFF7119 at 37.567 kb on - strand, within GFF7119 at 37.567 kb on - strand, within GFF7119 at 37.611 kb on + strand, within GFF7119 at 37.611 kb on + strand, within GFF7119 at 37.611 kb on + strand, within GFF7119 at 37.638 kb on + strand at 37.638 kb on + strand at 37.639 kb on - strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.684 kb on + strand at 37.685 kb on - strand at 37.685 kb on - strand at 37.840 kb on + strand, within GFF7120 at 37.840 kb on + strand, within GFF7120 at 37.840 kb on + strand, within GFF7120 at 37.840 kb on + strand, within GFF7120 at 37.841 kb on - strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.919 kb on - strand, within GFF7120 at 37.919 kb on - strand, within GFF7120 at 37.919 kb on - strand, within GFF7120 at 37.919 kb on - strand, within GFF7120 at 38.092 kb on - strand, within GFF7120 at 38.119 kb on + strand, within GFF7120 at 38.119 kb on + strand, within GFF7120 at 38.119 kb on + strand, within GFF7120 at 38.119 kb on + strand, within GFF7120 at 38.120 kb on - strand, within GFF7120 at 38.120 kb on - strand, within GFF7120 at 38.120 kb on - strand, within GFF7120
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 35,315 + GFF7116 0.89 +0.2 35,315 + GFF7116 0.89 -0.9 35,316 - GFF7116 0.89 +0.1 35,316 - GFF7116 0.89 -1.7 35,316 - GFF7116 0.89 +0.5 35,316 - GFF7116 0.89 -0.5 35,357 + -0.7 35,357 + +0.7 35,358 - -2.2 35,582 + GFF7117 0.21 -1.1 35,583 + GFF7117 0.21 +0.8 35,583 + GFF7117 0.21 -1.6 35,583 + GFF7117 0.21 -0.3 35,583 + GFF7117 0.21 -0.3 35,583 + GFF7117 0.21 -0.3 35,583 + GFF7117 0.21 +0.2 35,584 - GFF7117 0.21 -0.3 35,584 - GFF7117 0.21 -0.7 35,584 - GFF7117 0.21 -0.4 35,584 - GFF7117 0.21 +0.2 35,584 - GFF7117 0.21 -0.1 35,584 - GFF7117 0.21 -0.2 35,584 - GFF7117 0.21 -0.3 35,584 - GFF7117 0.21 +0.5 35,584 - GFF7117 0.21 +0.8 35,584 - GFF7117 0.21 -0.7 35,584 - GFF7117 0.21 +1.8 35,771 + GFF7117 0.44 -0.3 35,771 + GFF7117 0.44 +0.3 35,771 + GFF7117 0.44 +0.2 35,771 + GFF7117 0.44 -0.5 35,771 + GFF7117 0.44 -0.2 35,771 + GFF7117 0.44 -0.9 35,771 + GFF7117 0.44 +2.1 35,772 - GFF7117 0.44 -0.6 35,772 - GFF7117 0.44 -0.0 35,772 - GFF7117 0.44 +0.9 35,772 - GFF7117 0.44 +0.2 35,797 + GFF7117 0.47 -0.2 35,971 + GFF7117 0.68 -0.5 35,992 + GFF7117 0.70 -0.7 35,992 + GFF7117 0.70 -0.5 35,992 + GFF7117 0.70 -0.0 35,992 + GFF7117 0.70 -0.4 35,993 - GFF7117 0.70 +0.1 35,993 - GFF7117 0.70 -0.2 36,004 + GFF7117 0.72 +0.6 36,094 + GFF7117 0.82 -0.4 36,094 + GFF7117 0.82 -0.4 36,095 - GFF7117 0.83 +1.3 36,095 - GFF7117 0.83 -0.5 36,095 - GFF7117 0.83 +0.1 36,095 - GFF7117 0.83 +1.5 36,109 + GFF7117 0.84 -0.4 36,175 + -1.5 36,175 + +1.3 36,175 + -1.3 36,176 - -1.2 36,176 - -1.6 36,176 - +0.3 36,176 - -0.3 36,186 + -1.2 36,186 + -0.8 36,186 + -0.6 36,186 + -0.0 36,187 - +0.6 36,230 + -1.7 36,230 + +0.5 36,230 + -2.0 36,230 + -0.3 36,230 + +0.2 36,230 + -0.0 36,231 - -1.5 36,231 - -0.3 36,231 - -0.3 36,447 + GFF7118 0.22 -0.4 36,447 + GFF7118 0.22 +1.5 36,447 + GFF7118 0.22 +1.1 36,448 - GFF7118 0.23 -1.4 36,448 - GFF7118 0.23 -2.3 36,448 - GFF7118 0.23 -0.4 36,921 - GFF7118 0.73 +0.8 36,924 + GFF7118 0.73 -0.4 36,924 + GFF7118 0.73 -0.5 36,924 + GFF7118 0.73 -0.0 36,925 - GFF7118 0.73 +0.2 36,925 - GFF7118 0.73 +0.5 36,925 - GFF7118 0.73 -1.1 36,925 - GFF7118 0.73 -1.5 36,925 - GFF7118 0.73 -0.6 36,925 - GFF7118 0.73 -0.2 36,925 - GFF7118 0.73 -1.0 36,925 - GFF7118 0.73 -0.5 36,925 - GFF7118 0.73 +0.1 36,972 + GFF7118 0.78 +0.2 36,972 + GFF7118 0.78 -0.9 36,972 + GFF7118 0.78 -0.7 36,972 + GFF7118 0.78 +0.1 36,973 - GFF7118 0.78 -0.5 36,973 - GFF7118 0.78 -0.4 37,032 + GFF7118 0.85 +0.1 37,185 + +0.9 37,185 + -0.7 37,290 + GFF7119 0.22 +0.7 37,290 + GFF7119 0.22 -0.2 37,290 + GFF7119 0.22 +0.2 37,290 + GFF7119 0.22 +1.1 37,290 + GFF7119 0.22 +0.4 37,290 + GFF7119 0.22 -1.7 37,291 - GFF7119 0.22 -0.5 37,291 - GFF7119 0.22 +1.4 37,291 - GFF7119 0.22 +0.4 37,431 + GFF7119 0.52 -0.9 37,431 + GFF7119 0.52 -0.5 37,431 + GFF7119 0.52 -0.4 37,431 + GFF7119 0.52 -0.5 37,431 + GFF7119 0.52 -0.1 37,431 + GFF7119 0.52 -0.7 37,431 + GFF7119 0.52 -1.0 37,431 + GFF7119 0.52 +0.3 37,432 - GFF7119 0.52 -1.5 37,432 - GFF7119 0.52 -0.8 37,432 - GFF7119 0.52 -1.9 37,432 - GFF7119 0.52 -0.6 37,432 - GFF7119 0.52 -1.4 37,432 - GFF7119 0.52 -0.0 37,432 - GFF7119 0.52 +0.1 37,432 - GFF7119 0.52 +0.0 37,491 + GFF7119 0.64 +0.1 37,491 + GFF7119 0.64 +0.4 37,491 + GFF7119 0.64 +0.4 37,491 + GFF7119 0.64 +0.1 37,491 + GFF7119 0.64 +0.5 37,491 + GFF7119 0.64 -1.5 37,491 + GFF7119 0.64 +0.6 37,491 + GFF7119 0.64 +0.2 37,491 + GFF7119 0.64 -0.8 37,491 + GFF7119 0.64 -0.5 37,491 + GFF7119 0.64 -0.4 37,491 + GFF7119 0.64 -0.6 37,491 + GFF7119 0.64 +1.0 37,491 + GFF7119 0.64 +0.6 37,491 + GFF7119 0.64 +0.3 37,491 + GFF7119 0.64 -1.4 37,491 + GFF7119 0.64 -0.1 37,491 + GFF7119 0.64 -0.5 37,492 - GFF7119 0.65 +1.8 37,492 - GFF7119 0.65 +0.6 37,492 - GFF7119 0.65 -0.4 37,492 - GFF7119 0.65 -0.1 37,492 - GFF7119 0.65 -0.4 37,492 - GFF7119 0.65 -1.1 37,492 - GFF7119 0.65 -0.6 37,492 - GFF7119 0.65 -1.1 37,492 - GFF7119 0.65 -0.5 37,492 - GFF7119 0.65 +0.6 37,492 - GFF7119 0.65 -1.7 37,492 - GFF7119 0.65 -1.3 37,492 - GFF7119 0.65 +1.8 37,492 - GFF7119 0.65 -0.9 37,492 - GFF7119 0.65 -0.0 37,492 - GFF7119 0.65 -0.2 37,492 - GFF7119 0.65 -0.6 37,492 - GFF7119 0.65 -0.4 37,492 - GFF7119 0.65 -1.2 37,492 - GFF7119 0.65 -0.1 37,492 - GFF7119 0.65 -0.3 37,545 + GFF7119 0.76 +0.5 37,546 - GFF7119 0.76 -0.2 37,546 - GFF7119 0.76 -0.0 37,567 - GFF7119 0.80 -0.4 37,567 - GFF7119 0.80 +0.4 37,611 + GFF7119 0.90 -0.7 37,611 + GFF7119 0.90 +0.2 37,611 + GFF7119 0.90 -0.6 37,638 + -0.0 37,638 + -1.2 37,639 - +0.0 37,675 + +1.4 37,675 + -1.5 37,675 + -1.2 37,675 + -2.0 37,675 + -1.0 37,675 + +0.1 37,675 + +1.2 37,675 + -0.3 37,675 + +0.0 37,675 + -1.1 37,676 - -1.0 37,676 - -0.4 37,676 - +0.1 37,676 - -0.8 37,676 - +0.8 37,676 - +0.8 37,676 - +1.8 37,676 - +0.2 37,676 - +0.3 37,676 - -0.1 37,676 - -1.0 37,684 + +0.2 37,685 - -1.4 37,685 - -0.6 37,840 + GFF7120 0.14 -0.7 37,840 + GFF7120 0.14 -0.1 37,840 + GFF7120 0.14 -0.0 37,840 + GFF7120 0.14 -1.0 37,841 - GFF7120 0.14 -0.6 37,918 + GFF7120 0.21 -0.4 37,918 + GFF7120 0.21 +1.2 37,918 + GFF7120 0.21 -1.3 37,918 + GFF7120 0.21 +0.1 37,918 + GFF7120 0.21 -0.1 37,918 + GFF7120 0.21 -0.6 37,919 - GFF7120 0.21 -0.3 37,919 - GFF7120 0.21 +0.9 37,919 - GFF7120 0.21 -0.6 37,919 - GFF7120 0.21 -0.8 38,092 - GFF7120 0.35 -0.3 38,119 + GFF7120 0.37 -0.2 38,119 + GFF7120 0.37 -0.4 38,119 + GFF7120 0.37 -2.9 38,119 + GFF7120 0.37 +0.6 38,120 - GFF7120 0.37 -1.0 38,120 - GFF7120 0.37 -0.2 38,120 - GFF7120 0.37 -0.8
Or see this region's nucleotide sequence