Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7064 and GFF7065 are separated by 68 nucleotides GFF7065 and GFF7066 overlap by 4 nucleotides GFF7066 and GFF7067 are separated by 132 nucleotides GFF7067 and GFF7068 are separated by 117 nucleotides GFF7068 and GFF7069 are separated by 10 nucleotides
GFF7064 - Glutamate--cysteine ligase (EC 6.3.2.2), at 22,571 to 24,076
GFF7064
GFF7065 - no description, at 24,145 to 24,279
GFF7065
GFF7066 - Two-component transcriptional response regulator receiver domain, at 24,276 to 24,656
GFF7066
GFF7067 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 24,789 to 25,718
GFF7067
GFF7068 - hypothetical protein, at 25,836 to 26,258
GFF7068
GFF7069 - Transcriptional regulator, at 26,269 to 26,736
GFF7069
Position (kb)
24
25
26 Strain fitness (log2 ratio)
-2
-1
0
1 at 23.843 kb on + strand, within GFF7064 at 24.005 kb on + strand at 24.005 kb on + strand at 24.005 kb on + strand at 24.005 kb on + strand at 24.006 kb on - strand at 24.006 kb on - strand at 24.017 kb on + strand at 24.017 kb on + strand at 24.017 kb on + strand at 24.017 kb on + strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.074 kb on + strand at 24.075 kb on - strand at 24.151 kb on + strand at 24.151 kb on + strand at 24.152 kb on - strand at 24.152 kb on - strand at 24.241 kb on - strand, within GFF7065 at 24.291 kb on + strand at 24.291 kb on + strand at 24.292 kb on - strand at 24.292 kb on - strand at 24.534 kb on + strand, within GFF7066 at 24.534 kb on + strand, within GFF7066 at 24.534 kb on + strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.624 kb on + strand at 24.624 kb on + strand at 24.625 kb on - strand at 24.770 kb on - strand at 25.233 kb on + strand, within GFF7067 at 25.272 kb on + strand, within GFF7067 at 25.296 kb on + strand, within GFF7067 at 25.296 kb on + strand, within GFF7067 at 25.296 kb on + strand, within GFF7067 at 25.297 kb on - strand, within GFF7067 at 25.297 kb on - strand, within GFF7067 at 25.297 kb on - strand, within GFF7067 at 25.297 kb on - strand, within GFF7067 at 25.428 kb on + strand, within GFF7067 at 25.771 kb on - strand at 25.974 kb on + strand, within GFF7068 at 25.974 kb on + strand, within GFF7068 at 25.974 kb on + strand, within GFF7068 at 25.974 kb on + strand, within GFF7068 at 25.974 kb on + strand, within GFF7068 at 25.974 kb on + strand, within GFF7068 at 25.975 kb on - strand, within GFF7068 at 25.975 kb on - strand, within GFF7068 at 25.975 kb on - strand, within GFF7068 at 25.975 kb on - strand, within GFF7068 at 26.142 kb on + strand, within GFF7068 at 26.148 kb on + strand, within GFF7068 at 26.148 kb on + strand, within GFF7068 at 26.148 kb on + strand, within GFF7068 at 26.148 kb on + strand, within GFF7068 at 26.148 kb on + strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.149 kb on - strand, within GFF7068 at 26.193 kb on + strand, within GFF7068 at 26.193 kb on + strand, within GFF7068 at 26.332 kb on + strand, within GFF7069 at 26.332 kb on + strand, within GFF7069 at 26.389 kb on + strand, within GFF7069 at 26.389 kb on + strand, within GFF7069 at 26.390 kb on - strand, within GFF7069 at 26.471 kb on - strand, within GFF7069 at 26.608 kb on + strand, within GFF7069 at 26.609 kb on - strand, within GFF7069
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 23,843 + GFF7064 0.84 -0.4 24,005 + -0.4 24,005 + +0.5 24,005 + -0.0 24,005 + -0.4 24,006 - -0.2 24,006 - +0.5 24,017 + -1.0 24,017 + +0.5 24,017 + -1.9 24,017 + -0.3 24,018 - -0.6 24,018 - -0.5 24,018 - +0.7 24,018 - -1.8 24,018 - -0.0 24,018 - +0.1 24,018 - +0.1 24,018 - -0.1 24,018 - -0.2 24,018 - +0.1 24,018 - +0.5 24,018 - +0.6 24,074 + -0.7 24,075 - +0.0 24,151 + -2.0 24,151 + +1.5 24,152 - -0.3 24,152 - +0.4 24,241 - GFF7065 0.71 -0.8 24,291 + +0.2 24,291 + -0.3 24,292 - -0.4 24,292 - -0.3 24,534 + GFF7066 0.68 -0.2 24,534 + GFF7066 0.68 +0.1 24,534 + GFF7066 0.68 -0.3 24,535 - GFF7066 0.68 -0.8 24,535 - GFF7066 0.68 +0.0 24,535 - GFF7066 0.68 -0.4 24,535 - GFF7066 0.68 -0.4 24,535 - GFF7066 0.68 -1.0 24,535 - GFF7066 0.68 -0.4 24,535 - GFF7066 0.68 +0.6 24,624 + -0.8 24,624 + -1.1 24,625 - -0.9 24,770 - -0.1 25,233 + GFF7067 0.48 -0.6 25,272 + GFF7067 0.52 +0.1 25,296 + GFF7067 0.55 +0.5 25,296 + GFF7067 0.55 -0.4 25,296 + GFF7067 0.55 -0.3 25,297 - GFF7067 0.55 -0.1 25,297 - GFF7067 0.55 +0.7 25,297 - GFF7067 0.55 +0.1 25,297 - GFF7067 0.55 +0.1 25,428 + GFF7067 0.69 +1.5 25,771 - +0.1 25,974 + GFF7068 0.33 -0.2 25,974 + GFF7068 0.33 +0.1 25,974 + GFF7068 0.33 +0.9 25,974 + GFF7068 0.33 -0.1 25,974 + GFF7068 0.33 -0.0 25,974 + GFF7068 0.33 -0.2 25,975 - GFF7068 0.33 -0.1 25,975 - GFF7068 0.33 -0.3 25,975 - GFF7068 0.33 -0.0 25,975 - GFF7068 0.33 -1.5 26,142 + GFF7068 0.72 -1.5 26,148 + GFF7068 0.74 -0.5 26,148 + GFF7068 0.74 +1.5 26,148 + GFF7068 0.74 -0.9 26,148 + GFF7068 0.74 +0.3 26,148 + GFF7068 0.74 +0.7 26,149 - GFF7068 0.74 +0.2 26,149 - GFF7068 0.74 +0.2 26,149 - GFF7068 0.74 +1.1 26,149 - GFF7068 0.74 +0.3 26,149 - GFF7068 0.74 -0.7 26,149 - GFF7068 0.74 +0.7 26,149 - GFF7068 0.74 -0.4 26,149 - GFF7068 0.74 -1.1 26,149 - GFF7068 0.74 -0.2 26,193 + GFF7068 0.84 +0.3 26,193 + GFF7068 0.84 -1.4 26,332 + GFF7069 0.13 +0.5 26,332 + GFF7069 0.13 +0.8 26,389 + GFF7069 0.26 +1.0 26,389 + GFF7069 0.26 -0.4 26,390 - GFF7069 0.26 -1.3 26,471 - GFF7069 0.43 +0.3 26,608 + GFF7069 0.72 -1.3 26,609 - GFF7069 0.73 +1.5
Or see this region's nucleotide sequence