Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6981 and GFF6982 are separated by 7 nucleotides GFF6982 and GFF6983 are separated by 26 nucleotides GFF6983 and GFF6984 are separated by 34 nucleotides GFF6984 and GFF6985 are separated by 177 nucleotides
GFF6981 - hypothetical protein, at 381 to 956
GFF6981
GFF6982 - hypothetical protein, at 964 to 1,443
GFF6982
GFF6983 - FIG140336: TPR domain protein, at 1,470 to 2,747
GFF6983
GFF6984 - RNA polymerase sigma factor for flagellar operon, at 2,782 to 3,468
GFF6984
GFF6985 - Flagellar biosynthesis protein FliP, at 3,646 to 4,419
GFF6985
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1 at 0.621 kb on + strand, within GFF6981 at 0.674 kb on + strand, within GFF6981 at 0.781 kb on - strand, within GFF6981 at 0.909 kb on - strand at 0.930 kb on + strand at 1.040 kb on + strand, within GFF6982 at 1.040 kb on + strand, within GFF6982 at 1.257 kb on - strand, within GFF6982 at 1.257 kb on - strand, within GFF6982 at 1.509 kb on + strand at 1.510 kb on - strand at 1.510 kb on - strand at 1.724 kb on - strand, within GFF6983 at 1.724 kb on - strand, within GFF6983 at 1.724 kb on - strand, within GFF6983 at 2.386 kb on + strand, within GFF6983 at 2.386 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.560 kb on + strand, within GFF6983 at 2.561 kb on - strand, within GFF6983 at 2.739 kb on - strand at 2.762 kb on + strand at 2.921 kb on + strand, within GFF6984 at 2.922 kb on - strand, within GFF6984 at 2.980 kb on + strand, within GFF6984 at 3.022 kb on + strand, within GFF6984 at 3.022 kb on + strand, within GFF6984 at 3.023 kb on - strand, within GFF6984 at 3.023 kb on - strand, within GFF6984 at 3.131 kb on + strand, within GFF6984 at 3.382 kb on + strand, within GFF6984 at 3.410 kb on + strand at 3.557 kb on - strand at 3.557 kb on - strand at 3.557 kb on - strand at 3.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 621 + GFF6981 0.42 +0.5 674 + GFF6981 0.51 +0.5 781 - GFF6981 0.69 +1.0 909 - -0.5 930 + -0.2 1,040 + GFF6982 0.16 +0.1 1,040 + GFF6982 0.16 +0.6 1,257 - GFF6982 0.61 -0.1 1,257 - GFF6982 0.61 -1.6 1,509 + -0.3 1,510 - -0.3 1,510 - +0.2 1,724 - GFF6983 0.20 +0.4 1,724 - GFF6983 0.20 -0.2 1,724 - GFF6983 0.20 +0.5 2,386 + GFF6983 0.72 -2.0 2,386 + GFF6983 0.72 -0.2 2,560 + GFF6983 0.85 +0.1 2,560 + GFF6983 0.85 -1.3 2,560 + GFF6983 0.85 +0.6 2,561 - GFF6983 0.85 +1.3 2,739 - -0.6 2,762 + +0.3 2,921 + GFF6984 0.20 -0.7 2,922 - GFF6984 0.20 +0.6 2,980 + GFF6984 0.29 +0.6 3,022 + GFF6984 0.35 -0.9 3,022 + GFF6984 0.35 -0.8 3,023 - GFF6984 0.35 -1.4 3,023 - GFF6984 0.35 -0.5 3,131 + GFF6984 0.51 -1.3 3,382 + GFF6984 0.87 -0.6 3,410 + -0.1 3,557 - +0.3 3,557 - -0.3 3,557 - -0.3 3,634 - -0.4
Or see this region's nucleotide sequence