Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6745 and GFF6746 overlap by 8 nucleotides GFF6746 and GFF6747 overlap by 4 nucleotides
GFF6745 - hypothetical protein, at 58,675 to 59,538
GFF6745
GFF6746 - dolichyl-phosphate-mannose-protein mannosyltransferase family protein, at 59,531 to 61,147
GFF6746
GFF6747 - Glycosyl transferase, family 2, at 61,144 to 62,229
GFF6747
Position (kb) 59
60
61
62 Strain fitness (log2 ratio) -2
-1
0
1
2 at 58.538 kb on + strand at 58.556 kb on + strand at 58.557 kb on - strand at 58.676 kb on + strand at 58.676 kb on + strand at 58.676 kb on + strand at 58.684 kb on + strand at 58.684 kb on + strand at 58.685 kb on - strand at 58.685 kb on - strand at 58.899 kb on - strand, within GFF6745 at 58.925 kb on + strand, within GFF6745 at 58.926 kb on - strand, within GFF6745 at 58.926 kb on - strand, within GFF6745 at 58.926 kb on - strand, within GFF6745 at 59.057 kb on + strand, within GFF6745 at 59.159 kb on + strand, within GFF6745 at 59.160 kb on - strand, within GFF6745 at 59.220 kb on - strand, within GFF6745 at 59.220 kb on - strand, within GFF6745 at 59.249 kb on + strand, within GFF6745 at 59.250 kb on - strand, within GFF6745 at 59.250 kb on - strand, within GFF6745 at 59.771 kb on + strand, within GFF6746 at 59.771 kb on + strand, within GFF6746 at 59.771 kb on + strand, within GFF6746 at 59.771 kb on + strand, within GFF6746 at 59.772 kb on - strand, within GFF6746 at 59.832 kb on + strand, within GFF6746 at 59.832 kb on + strand, within GFF6746 at 59.833 kb on - strand, within GFF6746 at 59.833 kb on - strand, within GFF6746 at 59.919 kb on + strand, within GFF6746 at 59.935 kb on - strand, within GFF6746 at 59.937 kb on + strand, within GFF6746 at 59.938 kb on - strand, within GFF6746 at 59.938 kb on - strand, within GFF6746 at 59.938 kb on - strand, within GFF6746 at 59.938 kb on - strand, within GFF6746 at 59.938 kb on - strand, within GFF6746 at 59.940 kb on + strand, within GFF6746 at 59.940 kb on + strand, within GFF6746 at 59.940 kb on + strand, within GFF6746 at 59.940 kb on + strand, within GFF6746 at 59.940 kb on + strand, within GFF6746 at 59.941 kb on - strand, within GFF6746 at 59.941 kb on - strand, within GFF6746 at 59.941 kb on - strand, within GFF6746 at 59.941 kb on - strand, within GFF6746 at 59.941 kb on - strand, within GFF6746 at 59.941 kb on - strand, within GFF6746 at 60.024 kb on + strand, within GFF6746 at 60.342 kb on - strand, within GFF6746 at 60.342 kb on - strand, within GFF6746 at 60.342 kb on - strand, within GFF6746 at 60.345 kb on + strand, within GFF6746 at 60.345 kb on + strand, within GFF6746 at 60.346 kb on - strand, within GFF6746 at 60.346 kb on - strand, within GFF6746 at 60.417 kb on + strand, within GFF6746 at 60.417 kb on + strand, within GFF6746 at 60.418 kb on - strand, within GFF6746 at 60.418 kb on - strand, within GFF6746 at 60.418 kb on - strand, within GFF6746 at 60.436 kb on - strand, within GFF6746 at 60.621 kb on + strand, within GFF6746 at 60.622 kb on - strand, within GFF6746 at 60.930 kb on + strand, within GFF6746 at 60.930 kb on + strand, within GFF6746 at 60.931 kb on - strand, within GFF6746 at 61.199 kb on + strand at 61.199 kb on + strand at 61.199 kb on + strand at 61.226 kb on + strand at 61.241 kb on + strand at 61.241 kb on + strand at 61.241 kb on + strand at 61.241 kb on + strand at 61.242 kb on - strand at 61.358 kb on + strand, within GFF6747 at 61.358 kb on + strand, within GFF6747 at 61.359 kb on - strand, within GFF6747 at 61.553 kb on + strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.682 kb on + strand, within GFF6747 at 61.682 kb on + strand, within GFF6747 at 61.683 kb on - strand, within GFF6747 at 61.683 kb on - strand, within GFF6747 at 61.745 kb on + strand, within GFF6747 at 61.745 kb on + strand, within GFF6747 at 61.746 kb on - strand, within GFF6747 at 61.746 kb on - strand, within GFF6747 at 61.746 kb on - strand, within GFF6747 at 62.063 kb on + strand, within GFF6747 at 62.064 kb on - strand, within GFF6747
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 58,538 + +1.3 58,556 + -1.6 58,557 - -0.3 58,676 + -0.4 58,676 + +0.1 58,676 + +0.5 58,684 + -0.9 58,684 + +0.3 58,685 - +0.3 58,685 - -1.2 58,899 - GFF6745 0.26 -0.8 58,925 + GFF6745 0.29 -0.9 58,926 - GFF6745 0.29 -1.8 58,926 - GFF6745 0.29 -0.2 58,926 - GFF6745 0.29 -0.9 59,057 + GFF6745 0.44 +0.5 59,159 + GFF6745 0.56 -0.3 59,160 - GFF6745 0.56 -0.6 59,220 - GFF6745 0.63 +1.0 59,220 - GFF6745 0.63 -0.4 59,249 + GFF6745 0.66 +0.4 59,250 - GFF6745 0.67 +0.3 59,250 - GFF6745 0.67 -1.1 59,771 + GFF6746 0.15 +0.6 59,771 + GFF6746 0.15 +0.6 59,771 + GFF6746 0.15 +2.2 59,771 + GFF6746 0.15 -0.9 59,772 - GFF6746 0.15 +0.7 59,832 + GFF6746 0.19 +0.2 59,832 + GFF6746 0.19 -1.1 59,833 - GFF6746 0.19 +0.6 59,833 - GFF6746 0.19 +0.6 59,919 + GFF6746 0.24 +0.2 59,935 - GFF6746 0.25 -0.2 59,937 + GFF6746 0.25 +0.7 59,938 - GFF6746 0.25 +0.2 59,938 - GFF6746 0.25 -0.4 59,938 - GFF6746 0.25 -0.1 59,938 - GFF6746 0.25 +0.4 59,938 - GFF6746 0.25 -0.6 59,940 + GFF6746 0.25 -0.5 59,940 + GFF6746 0.25 +1.1 59,940 + GFF6746 0.25 -0.1 59,940 + GFF6746 0.25 +0.5 59,940 + GFF6746 0.25 -0.7 59,941 - GFF6746 0.25 +0.3 59,941 - GFF6746 0.25 -0.4 59,941 - GFF6746 0.25 -0.4 59,941 - GFF6746 0.25 +0.3 59,941 - GFF6746 0.25 +1.1 59,941 - GFF6746 0.25 +0.9 60,024 + GFF6746 0.30 -0.3 60,342 - GFF6746 0.50 +2.6 60,342 - GFF6746 0.50 +0.3 60,342 - GFF6746 0.50 +0.2 60,345 + GFF6746 0.50 +0.6 60,345 + GFF6746 0.50 +0.4 60,346 - GFF6746 0.50 +0.1 60,346 - GFF6746 0.50 -0.5 60,417 + GFF6746 0.55 -0.1 60,417 + GFF6746 0.55 -1.1 60,418 - GFF6746 0.55 -1.8 60,418 - GFF6746 0.55 -0.3 60,418 - GFF6746 0.55 -0.1 60,436 - GFF6746 0.56 +0.2 60,621 + GFF6746 0.67 -0.5 60,622 - GFF6746 0.67 -0.7 60,930 + GFF6746 0.87 +0.6 60,930 + GFF6746 0.87 -0.1 60,931 - GFF6746 0.87 -0.4 61,199 + -1.5 61,199 + +0.1 61,199 + +0.4 61,226 + -0.2 61,241 + +0.1 61,241 + +0.3 61,241 + -0.6 61,241 + -0.7 61,242 - -0.4 61,358 + GFF6747 0.20 +0.6 61,358 + GFF6747 0.20 -0.1 61,359 - GFF6747 0.20 +1.4 61,553 + GFF6747 0.38 -0.4 61,554 - GFF6747 0.38 -0.2 61,554 - GFF6747 0.38 +1.2 61,554 - GFF6747 0.38 -0.3 61,554 - GFF6747 0.38 +0.6 61,554 - GFF6747 0.38 +0.8 61,682 + GFF6747 0.50 -0.2 61,682 + GFF6747 0.50 -0.4 61,683 - GFF6747 0.50 +0.9 61,683 - GFF6747 0.50 +1.6 61,745 + GFF6747 0.55 -0.3 61,745 + GFF6747 0.55 -0.1 61,746 - GFF6747 0.55 +0.2 61,746 - GFF6747 0.55 -0.4 61,746 - GFF6747 0.55 -0.6 62,063 + GFF6747 0.85 -0.4 62,064 - GFF6747 0.85 +0.6
Or see this region's nucleotide sequence