Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6673 and GFF6674 are separated by 13 nucleotides GFF6674 and GFF6675 are separated by 16 nucleotides GFF6675 and GFF6676 are separated by 82 nucleotides
GFF6673 - Membrane protein, at 48,695 to 49,264
GFF6673
GFF6674 - Predicted membrane protein, at 49,278 to 49,682
GFF6674
GFF6675 - ortholog of Bordetella pertussis (BX470248) BP3300, at 49,699 to 50,634
GFF6675
GFF6676 - Ammonium transporter, at 50,717 to 51,919
GFF6676
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 48.756 kb on + strand, within GFF6673 at 48.757 kb on - strand, within GFF6673 at 48.757 kb on - strand, within GFF6673 at 48.757 kb on - strand, within GFF6673 at 48.770 kb on + strand, within GFF6673 at 48.771 kb on - strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.965 kb on + strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 48.966 kb on - strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.106 kb on + strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.107 kb on - strand, within GFF6673 at 49.118 kb on + strand, within GFF6673 at 49.118 kb on + strand, within GFF6673 at 49.118 kb on + strand, within GFF6673 at 49.208 kb on + strand at 49.208 kb on + strand at 49.208 kb on + strand at 49.209 kb on - strand at 49.209 kb on - strand at 49.262 kb on + strand at 49.262 kb on + strand at 49.262 kb on + strand at 49.263 kb on - strand at 49.263 kb on - strand at 49.307 kb on + strand at 49.308 kb on - strand at 49.369 kb on + strand, within GFF6674 at 49.369 kb on + strand, within GFF6674 at 49.369 kb on + strand, within GFF6674 at 49.369 kb on + strand, within GFF6674 at 49.370 kb on - strand, within GFF6674 at 49.370 kb on - strand, within GFF6674 at 49.370 kb on - strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.408 kb on + strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.409 kb on - strand, within GFF6674 at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.718 kb on + strand at 49.719 kb on - strand at 49.719 kb on - strand at 49.719 kb on - strand at 49.719 kb on - strand at 49.719 kb on - strand at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.889 kb on + strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.890 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.965 kb on - strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.979 kb on + strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 49.980 kb on - strand, within GFF6675 at 50.081 kb on + strand, within GFF6675 at 50.186 kb on + strand, within GFF6675 at 50.186 kb on + strand, within GFF6675 at 50.390 kb on + strand, within GFF6675 at 50.390 kb on + strand, within GFF6675 at 50.390 kb on + strand, within GFF6675 at 50.391 kb on - strand, within GFF6675 at 50.555 kb on + strand at 50.555 kb on + strand at 50.555 kb on + strand at 50.555 kb on + strand at 50.555 kb on + strand at 50.555 kb on + strand at 50.556 kb on + strand at 50.556 kb on - strand at 50.556 kb on - strand at 50.556 kb on - strand at 50.556 kb on - strand at 50.556 kb on - strand at 50.674 kb on + strand at 50.674 kb on + strand at 50.674 kb on + strand at 50.675 kb on - strand at 50.675 kb on - strand at 50.675 kb on - strand at 50.675 kb on - strand at 50.675 kb on - strand at 50.752 kb on + strand at 50.752 kb on + strand at 50.752 kb on + strand at 50.752 kb on + strand at 50.752 kb on + strand at 50.752 kb on + strand at 50.753 kb on - strand at 50.781 kb on + strand at 50.781 kb on + strand at 50.782 kb on - strand at 50.782 kb on - strand at 50.832 kb on + strand at 50.832 kb on + strand at 50.832 kb on + strand at 50.832 kb on + strand at 50.832 kb on + strand at 50.832 kb on + strand at 50.833 kb on - strand at 50.904 kb on - strand, within GFF6676 at 51.147 kb on + strand, within GFF6676 at 51.147 kb on + strand, within GFF6676 at 51.147 kb on + strand, within GFF6676 at 51.147 kb on + strand, within GFF6676 at 51.148 kb on - strand, within GFF6676 at 51.148 kb on - strand, within GFF6676 at 51.528 kb on + strand, within GFF6676 at 51.529 kb on - strand, within GFF6676 at 51.529 kb on - strand, within GFF6676 at 51.529 kb on - strand, within GFF6676 at 51.529 kb on - strand, within GFF6676 at 51.529 kb on - strand, within GFF6676 at 51.529 kb on - strand, within GFF6676 at 51.529 kb on - strand, within GFF6676 at 51.529 kb on - strand, within GFF6676 at 51.537 kb on + strand, within GFF6676
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 48,756 + GFF6673 0.11 -0.8 48,757 - GFF6673 0.11 -0.2 48,757 - GFF6673 0.11 +0.8 48,757 - GFF6673 0.11 -0.0 48,770 + GFF6673 0.13 -1.6 48,771 - GFF6673 0.13 -0.2 48,965 + GFF6673 0.47 -0.8 48,965 + GFF6673 0.47 +0.4 48,965 + GFF6673 0.47 -0.9 48,965 + GFF6673 0.47 +0.1 48,965 + GFF6673 0.47 -0.9 48,965 + GFF6673 0.47 +0.6 48,965 + GFF6673 0.47 +0.4 48,965 + GFF6673 0.47 +1.1 48,965 + GFF6673 0.47 -0.9 48,965 + GFF6673 0.47 +0.4 48,965 + GFF6673 0.47 -2.0 48,965 + GFF6673 0.47 -1.9 48,965 + GFF6673 0.47 +0.4 48,965 + GFF6673 0.47 -0.7 48,965 + GFF6673 0.47 -0.4 48,966 - GFF6673 0.48 +0.4 48,966 - GFF6673 0.48 -1.4 48,966 - GFF6673 0.48 +0.4 48,966 - GFF6673 0.48 -0.3 48,966 - GFF6673 0.48 -0.4 48,966 - GFF6673 0.48 -1.0 49,106 + GFF6673 0.72 -0.2 49,106 + GFF6673 0.72 -2.3 49,106 + GFF6673 0.72 +0.1 49,106 + GFF6673 0.72 -2.6 49,106 + GFF6673 0.72 -0.4 49,107 - GFF6673 0.72 +0.0 49,107 - GFF6673 0.72 -1.0 49,107 - GFF6673 0.72 +0.8 49,107 - GFF6673 0.72 +0.4 49,107 - GFF6673 0.72 -0.2 49,107 - GFF6673 0.72 -1.7 49,107 - GFF6673 0.72 +0.3 49,118 + GFF6673 0.74 +0.6 49,118 + GFF6673 0.74 +1.4 49,118 + GFF6673 0.74 -0.3 49,208 + +0.3 49,208 + +0.3 49,208 + -0.8 49,209 - +0.7 49,209 - +0.8 49,262 + -0.2 49,262 + -0.9 49,262 + -0.3 49,263 - -0.2 49,263 - -0.3 49,307 + -1.2 49,308 - -0.6 49,369 + GFF6674 0.22 +0.0 49,369 + GFF6674 0.22 -0.2 49,369 + GFF6674 0.22 +0.1 49,369 + GFF6674 0.22 +0.4 49,370 - GFF6674 0.23 -0.6 49,370 - GFF6674 0.23 -0.6 49,370 - GFF6674 0.23 +1.4 49,408 + GFF6674 0.32 +0.2 49,408 + GFF6674 0.32 -0.1 49,408 + GFF6674 0.32 +0.0 49,408 + GFF6674 0.32 +0.2 49,408 + GFF6674 0.32 -0.8 49,408 + GFF6674 0.32 -0.9 49,408 + GFF6674 0.32 -0.2 49,409 - GFF6674 0.32 -0.6 49,409 - GFF6674 0.32 -0.9 49,409 - GFF6674 0.32 +0.4 49,409 - GFF6674 0.32 -0.6 49,409 - GFF6674 0.32 +0.1 49,409 - GFF6674 0.32 +1.1 49,409 - GFF6674 0.32 +1.2 49,409 - GFF6674 0.32 -0.8 49,409 - GFF6674 0.32 -1.1 49,409 - GFF6674 0.32 -1.5 49,718 + +0.8 49,718 + +0.2 49,718 + -0.1 49,718 + +0.1 49,718 + -0.9 49,718 + +0.4 49,718 + +1.0 49,718 + -0.2 49,718 + -0.2 49,718 + +0.8 49,718 + +0.3 49,718 + -1.4 49,719 - -1.4 49,719 - -0.6 49,719 - +0.7 49,719 - -0.8 49,719 - -0.2 49,889 + GFF6675 0.20 -1.0 49,889 + GFF6675 0.20 +0.9 49,889 + GFF6675 0.20 -1.2 49,889 + GFF6675 0.20 -1.5 49,889 + GFF6675 0.20 -0.4 49,889 + GFF6675 0.20 +0.0 49,889 + GFF6675 0.20 -0.3 49,889 + GFF6675 0.20 -0.2 49,889 + GFF6675 0.20 +0.2 49,889 + GFF6675 0.20 +0.4 49,889 + GFF6675 0.20 +0.0 49,889 + GFF6675 0.20 -0.3 49,889 + GFF6675 0.20 -0.2 49,889 + GFF6675 0.20 -0.1 49,889 + GFF6675 0.20 +0.6 49,890 - GFF6675 0.20 -0.0 49,890 - GFF6675 0.20 +0.1 49,890 - GFF6675 0.20 -0.3 49,890 - GFF6675 0.20 -0.0 49,890 - GFF6675 0.20 +2.0 49,890 - GFF6675 0.20 -0.1 49,890 - GFF6675 0.20 +0.8 49,890 - GFF6675 0.20 -0.4 49,890 - GFF6675 0.20 +0.1 49,890 - GFF6675 0.20 +0.0 49,890 - GFF6675 0.20 -0.5 49,890 - GFF6675 0.20 -0.1 49,890 - GFF6675 0.20 +0.5 49,890 - GFF6675 0.20 +0.0 49,890 - GFF6675 0.20 -0.6 49,890 - GFF6675 0.20 +0.3 49,965 - GFF6675 0.28 -0.4 49,965 - GFF6675 0.28 -0.5 49,965 - GFF6675 0.28 +0.5 49,965 - GFF6675 0.28 -1.4 49,965 - GFF6675 0.28 -1.0 49,979 + GFF6675 0.30 +1.6 49,979 + GFF6675 0.30 -2.0 49,979 + GFF6675 0.30 -0.1 49,979 + GFF6675 0.30 -0.7 49,979 + GFF6675 0.30 +1.2 49,980 - GFF6675 0.30 +1.4 49,980 - GFF6675 0.30 -0.5 49,980 - GFF6675 0.30 -0.1 49,980 - GFF6675 0.30 +0.4 49,980 - GFF6675 0.30 -0.1 49,980 - GFF6675 0.30 +0.0 50,081 + GFF6675 0.41 +0.1 50,186 + GFF6675 0.52 -0.9 50,186 + GFF6675 0.52 -0.2 50,390 + GFF6675 0.74 +0.1 50,390 + GFF6675 0.74 -0.3 50,390 + GFF6675 0.74 -0.1 50,391 - GFF6675 0.74 +1.9 50,555 + -0.3 50,555 + +0.3 50,555 + -1.0 50,555 + -1.0 50,555 + +0.4 50,555 + +0.7 50,556 + -1.3 50,556 - -0.0 50,556 - +2.2 50,556 - +0.8 50,556 - -0.2 50,556 - -1.6 50,674 + +0.8 50,674 + -1.6 50,674 + +0.4 50,675 - -0.8 50,675 - +0.1 50,675 - -0.8 50,675 - -0.3 50,675 - -1.6 50,752 + -0.3 50,752 + +0.3 50,752 + -1.5 50,752 + -2.0 50,752 + -0.3 50,752 + -0.0 50,753 - +0.3 50,781 + -0.9 50,781 + -0.2 50,782 - -1.1 50,782 - -0.6 50,832 + -0.7 50,832 + -0.6 50,832 + -0.5 50,832 + -2.0 50,832 + +0.1 50,832 + +0.2 50,833 - -0.4 50,904 - GFF6676 0.16 -0.4 51,147 + GFF6676 0.36 +0.0 51,147 + GFF6676 0.36 +0.3 51,147 + GFF6676 0.36 +0.2 51,147 + GFF6676 0.36 +0.2 51,148 - GFF6676 0.36 -0.2 51,148 - GFF6676 0.36 -0.9 51,528 + GFF6676 0.67 -0.1 51,529 - GFF6676 0.67 +0.0 51,529 - GFF6676 0.67 -2.0 51,529 - GFF6676 0.67 -0.9 51,529 - GFF6676 0.67 -0.0 51,529 - GFF6676 0.67 -1.0 51,529 - GFF6676 0.67 -1.5 51,529 - GFF6676 0.67 -0.6 51,529 - GFF6676 0.67 -0.5 51,537 + GFF6676 0.68 -0.4
Or see this region's nucleotide sequence