Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6585 and GFF6586 overlap by 4 nucleotides GFF6586 and GFF6587 are separated by 30 nucleotides GFF6587 and GFF6588 are separated by 85 nucleotides
GFF6585 - D-glycerate 2-kinase (EC 2.7.1.165), at 26,854 to 28,179
GFF6585
GFF6586 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 28,176 to 29,084
GFF6586
GFF6587 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 29,115 to 29,894
GFF6587
GFF6588 - Glyoxylate carboligase (EC 4.1.1.47), at 29,980 to 31,770
GFF6588
Position (kb) 28
29
30 Strain fitness (log2 ratio) -1
0
1
2 at 27.210 kb on + strand, within GFF6585 at 27.519 kb on - strand, within GFF6585 at 27.849 kb on + strand, within GFF6585 at 27.951 kb on - strand, within GFF6585 at 27.967 kb on + strand, within GFF6585 at 27.968 kb on - strand, within GFF6585 at 27.968 kb on - strand, within GFF6585 at 27.996 kb on - strand, within GFF6585 at 28.069 kb on + strand at 28.641 kb on + strand, within GFF6586 at 28.642 kb on - strand, within GFF6586 at 28.723 kb on + strand, within GFF6586 at 28.723 kb on + strand, within GFF6586 at 28.979 kb on - strand, within GFF6586 at 28.979 kb on - strand, within GFF6586 at 29.086 kb on + strand at 29.086 kb on + strand at 29.086 kb on + strand at 29.086 kb on + strand at 29.087 kb on - strand at 29.087 kb on - strand at 29.087 kb on - strand at 29.087 kb on - strand at 29.087 kb on - strand at 29.116 kb on + strand at 29.117 kb on - strand at 29.173 kb on + strand at 29.174 kb on - strand at 29.309 kb on - strand, within GFF6587 at 29.309 kb on - strand, within GFF6587 at 29.309 kb on - strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.357 kb on - strand, within GFF6587 at 29.357 kb on - strand, within GFF6587 at 29.357 kb on - strand, within GFF6587 at 29.365 kb on + strand, within GFF6587 at 29.365 kb on + strand, within GFF6587 at 29.366 kb on - strand, within GFF6587 at 29.434 kb on + strand, within GFF6587 at 29.434 kb on + strand, within GFF6587 at 29.434 kb on + strand, within GFF6587 at 29.435 kb on - strand, within GFF6587 at 29.435 kb on - strand, within GFF6587 at 29.676 kb on + strand, within GFF6587 at 29.676 kb on + strand, within GFF6587 at 29.677 kb on - strand, within GFF6587 at 29.901 kb on + strand at 29.901 kb on + strand at 29.902 kb on - strand at 29.907 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 27,210 + GFF6585 0.27 +0.1 27,519 - GFF6585 0.50 +0.2 27,849 + GFF6585 0.75 -0.9 27,951 - GFF6585 0.83 +0.1 27,967 + GFF6585 0.84 -0.9 27,968 - GFF6585 0.84 +1.0 27,968 - GFF6585 0.84 -1.2 27,996 - GFF6585 0.86 +0.5 28,069 + +0.4 28,641 + GFF6586 0.51 -0.9 28,642 - GFF6586 0.51 -0.0 28,723 + GFF6586 0.60 +0.4 28,723 + GFF6586 0.60 +0.2 28,979 - GFF6586 0.88 +0.0 28,979 - GFF6586 0.88 -0.8 29,086 + +0.4 29,086 + -1.1 29,086 + -0.3 29,086 + +0.0 29,087 - +0.2 29,087 - -0.3 29,087 - -0.4 29,087 - -0.9 29,087 - +0.3 29,116 + +0.4 29,117 - +1.0 29,173 + +0.2 29,174 - -0.8 29,309 - GFF6587 0.25 -0.2 29,309 - GFF6587 0.25 +0.6 29,309 - GFF6587 0.25 +0.8 29,356 + GFF6587 0.31 -1.2 29,356 + GFF6587 0.31 -0.2 29,356 + GFF6587 0.31 -0.6 29,356 + GFF6587 0.31 +1.0 29,356 + GFF6587 0.31 -1.1 29,356 + GFF6587 0.31 +0.0 29,356 + GFF6587 0.31 -0.3 29,357 - GFF6587 0.31 +2.3 29,357 - GFF6587 0.31 +0.8 29,357 - GFF6587 0.31 -0.4 29,365 + GFF6587 0.32 -0.2 29,365 + GFF6587 0.32 -1.1 29,366 - GFF6587 0.32 -1.1 29,434 + GFF6587 0.41 +0.0 29,434 + GFF6587 0.41 +2.1 29,434 + GFF6587 0.41 +0.2 29,435 - GFF6587 0.41 +1.7 29,435 - GFF6587 0.41 -0.4 29,676 + GFF6587 0.72 +0.1 29,676 + GFF6587 0.72 -0.2 29,677 - GFF6587 0.72 +1.4 29,901 + +1.0 29,901 + -0.3 29,902 - -1.4 29,907 + -1.1
Or see this region's nucleotide sequence